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The map label for this gene is phrB [H]

Identifier: 123965553

GI number: 123965553

Start: 293860

End: 295296

Strand: Reverse

Name: phrB [H]

Synonym: P9515_03181

Alternate gene names: 123965553

Gene position: 295296-293860 (Counterclockwise)

Preceding gene: 123965554

Following gene: 123965550

Centisome position: 17.33

GC content: 29.58

Gene sequence:

>1437_bases
ATGAATAATCCCAGAATACTTTTTTGGCATAGAAAAGATCTTCGAATTTATGACAATAACGCTTTGTCAAAAGCATTTTC
TTTATCAAATGCTATTACTTCAACATATATTTTTGATCAAAATTATTCTCAAGACTTTAATGCGAATTCTAGAGCATGGT
TTCTTGGAAATTCATTGCAAGAATTAAGCAGAAATTGGGAAACCCTTGGTAGTCGAATGATTATAGATGAAGGTAATCCA
TTAACTCTCATACCTAAATTGGCTCAATTAATTGATGCTAAATTTGTTGCTTGGAATAAAGCAATAGAACCTTATGAAAT
TAATCGTGATTTAGAAATTAAAAATACTTTAAAAAAGTTTAAAATTGAGATTATAGAATTATGGGATCACTTATTATTAG
AACCTTCTCAAATTCATACTGGAAGTAACAAACCATATACAGTTTATGGTCCGTTTTACAAAAAATTAAAGTCAAAACTA
CAATTACTCACTTCGAAAAAAAATCTTAATAATATTGGTAAATTAAAGGATTTAGAAATAGGGATTAAAGAAAGTCAAAA
AATTGATAGTTCAAGATTAATATTAGAGAAATTTATAAAAAAAGTAAATTTTGATGGTATTAATTTATGTCCTTGCAAAC
CAGGAGAAATAGCTGCAGAAAAATTATTAGACCAATTTATTAACCAAGATAAAATTGGATCATACGCATCTTCAAGAGAC
TTTCCTGATCAAAACGGAACATCATTCCTAAGTGCATCATTGAGATTTGGGACAATAAGTATAAGAAAAGTCTGGAATTC
AACCTTGTTTACTGACTTAAAAAATAAAAATAACCATAATAAAATATCAATAGAAACTTGGCAAAAAGAACTAGTTTGGA
GAGAATTCTATCAACATTGCTTATTCCATTTTCCAGAATTAGAAAAAGGACCTTACAGAAAAAAATGGGATCATTTTCCT
TGGCAAAATAATCATGAATGGTTTGATCGATGGAGCAATGGAGAAACTGGAGTTCCTATTATCGATGCAGCAATGAGACA
ACTTAATAGTACAGGCTGGATGCATAATAGATGTCGAATGATAGTTGCTTCATTTCTCGTAAAAGATCTTATTTGCAATT
GGCAAATGGGTGAAGAAAAATTTATGAAGGTATTGGTGGATGGAGACTTAGCCGCAAATAATGGAGGTTGGCAATGGAGT
GCAAGTAGTGGCATGGATCCAAAGCCATTAAGAATTTTTAATCCATATACTCAAACTAAAAAATTCGATCCTATTTGCAA
ATATATTAAATTCTGGATTCCAGAATTATCTAAAGTACCTAATAAAGATATCCTAAATGGTGAGATTTCTGATTTGGAAA
AAAATAATTATTATGGAGCTATAGTTAATCACAATACTCAGCAAAGACTTTTTAAAGATCTTTACGCACATATTTGA

Upstream 100 bases:

>100_bases
ATCCTCAGAATTTAGATAATTTAATTTCTTGTGGTGATGAAGTCCTTGATGCAAAGACAGTAACAGCTTGGTTTAGAGCA
AAGCAATTTTTAGGAATTTA

Downstream 100 bases:

>100_bases
AGTTCGTCAATACATTTTTTTAAAATAATATTAAAGTTTTTAGATACATAAACTTGTTCTTCATAAGAAATTTCCGGATA
CATTGGAAGACTAAGTACTT

Product: putative DNA photolyase

Products: NA

Alternate protein names: DNA photolyase; Photoreactivating enzyme [H]

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MNNPRILFWHRKDLRIYDNNALSKAFSLSNAITSTYIFDQNYSQDFNANSRAWFLGNSLQELSRNWETLGSRMIIDEGNP
LTLIPKLAQLIDAKFVAWNKAIEPYEINRDLEIKNTLKKFKIEIIELWDHLLLEPSQIHTGSNKPYTVYGPFYKKLKSKL
QLLTSKKNLNNIGKLKDLEIGIKESQKIDSSRLILEKFIKKVNFDGINLCPCKPGEIAAEKLLDQFINQDKIGSYASSRD
FPDQNGTSFLSASLRFGTISIRKVWNSTLFTDLKNKNNHNKISIETWQKELVWREFYQHCLFHFPELEKGPYRKKWDHFP
WQNNHEWFDRWSNGETGVPIIDAAMRQLNSTGWMHNRCRMIVASFLVKDLICNWQMGEEKFMKVLVDGDLAANNGGWQWS
ASSGMDPKPLRIFNPYTQTKKFDPICKYIKFWIPELSKVPNKDILNGEISDLEKNNYYGAIVNHNTQQRLFKDLYAHI

Sequences:

>Translated_478_residues
MNNPRILFWHRKDLRIYDNNALSKAFSLSNAITSTYIFDQNYSQDFNANSRAWFLGNSLQELSRNWETLGSRMIIDEGNP
LTLIPKLAQLIDAKFVAWNKAIEPYEINRDLEIKNTLKKFKIEIIELWDHLLLEPSQIHTGSNKPYTVYGPFYKKLKSKL
QLLTSKKNLNNIGKLKDLEIGIKESQKIDSSRLILEKFIKKVNFDGINLCPCKPGEIAAEKLLDQFINQDKIGSYASSRD
FPDQNGTSFLSASLRFGTISIRKVWNSTLFTDLKNKNNHNKISIETWQKELVWREFYQHCLFHFPELEKGPYRKKWDHFP
WQNNHEWFDRWSNGETGVPIIDAAMRQLNSTGWMHNRCRMIVASFLVKDLICNWQMGEEKFMKVLVDGDLAANNGGWQWS
ASSGMDPKPLRIFNPYTQTKKFDPICKYIKFWIPELSKVPNKDILNGEISDLEKNNYYGAIVNHNTQQRLFKDLYAHI
>Mature_478_residues
MNNPRILFWHRKDLRIYDNNALSKAFSLSNAITSTYIFDQNYSQDFNANSRAWFLGNSLQELSRNWETLGSRMIIDEGNP
LTLIPKLAQLIDAKFVAWNKAIEPYEINRDLEIKNTLKKFKIEIIELWDHLLLEPSQIHTGSNKPYTVYGPFYKKLKSKL
QLLTSKKNLNNIGKLKDLEIGIKESQKIDSSRLILEKFIKKVNFDGINLCPCKPGEIAAEKLLDQFINQDKIGSYASSRD
FPDQNGTSFLSASLRFGTISIRKVWNSTLFTDLKNKNNHNKISIETWQKELVWREFYQHCLFHFPELEKGPYRKKWDHFP
WQNNHEWFDRWSNGETGVPIIDAAMRQLNSTGWMHNRCRMIVASFLVKDLICNWQMGEEKFMKVLVDGDLAANNGGWQWS
ASSGMDPKPLRIFNPYTQTKKFDPICKYIKFWIPELSKVPNKDILNGEISDLEKNNYYGAIVNHNTQQRLFKDLYAHI

Specific function: Involved in repair of UV radiation-induced DNA damage. Catalyzes the light-dependent monomerization (300-600 nm) of cyclobutyl pyrimidine dimers (in cis-syn configuration), which are formed between adjacent bases on the same DNA strand upon exposure to ul

COG id: COG0415

COG function: function code L; Deoxyribodipyrimidine photolyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DNA photolyase domain [H]

Homologues:

Organism=Homo sapiens, GI188536100, Length=498, Percent_Identity=25.7028112449799, Blast_Score=165, Evalue=8e-41,
Organism=Homo sapiens, GI4758072, Length=496, Percent_Identity=27.6209677419355, Blast_Score=162, Evalue=5e-40,
Organism=Homo sapiens, GI188536103, Length=456, Percent_Identity=25.219298245614, Blast_Score=146, Evalue=4e-35,
Organism=Escherichia coli, GI1786926, Length=450, Percent_Identity=37.3333333333333, Blast_Score=251, Evalue=5e-68,
Organism=Saccharomyces cerevisiae, GI6324962, Length=476, Percent_Identity=29.8319327731092, Blast_Score=189, Evalue=9e-49,
Organism=Drosophila melanogaster, GI17137248, Length=489, Percent_Identity=27.6073619631902, Blast_Score=191, Evalue=9e-49,
Organism=Drosophila melanogaster, GI24585455, Length=489, Percent_Identity=27.6073619631902, Blast_Score=191, Evalue=9e-49,
Organism=Drosophila melanogaster, GI24648152, Length=509, Percent_Identity=25.147347740668, Blast_Score=148, Evalue=8e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002081
- InterPro:   IPR018394
- InterPro:   IPR006050
- InterPro:   IPR019947
- InterPro:   IPR005101
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00875 DNA_photolyase; PF03441 FAD_binding_7 [H]

EC number: =4.1.99.3 [H]

Molecular weight: Translated: 55892; Mature: 55892

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: PS00394 DNA_PHOTOLYASES_1_1 ; PS00691 DNA_PHOTOLYASES_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNPRILFWHRKDLRIYDNNALSKAFSLSNAITSTYIFDQNYSQDFNANSRAWFLGNSLQ
CCCCCEEEEECCCCEEECCCHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCEEEECCHHH
ELSRNWETLGSRMIIDEGNPLTLIPKLAQLIDAKFVAWNKAIEPYEINRDLEIKNTLKKF
HHHHHHHHHCCEEEEECCCCEEHHHHHHHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHH
KIEIIELWDHLLLEPSQIHTGSNKPYTVYGPFYKKLKSKLQLLTSKKNLNNIGKLKDLEI
HHHHHHHHHHHHCCCHHHCCCCCCCEEEECHHHHHHHHHHHHHHHHHCCCCCCCCEEEEE
GIKESQKIDSSRLILEKFIKKVNFDGINLCPCKPGEIAAEKLLDQFINQDKIGSYASSRD
CCCHHHCCCHHHHHHHHHHHHCCCCCCEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCC
FPDQNGTSFLSASLRFGTISIRKVWNSTLFTDLKNKNNHNKISIETWQKELVWREFYQHC
CCCCCCCHHEEEEEEECEEHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHH
LFHFPELEKGPYRKKWDHFPWQNNHEWFDRWSNGETGVPIIDAAMRQLNSTGWMHNRCRM
HHCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHH
IVASFLVKDLICNWQMGEEKFMKVLVDGDLAANNGGWQWSASSGMDPKPLRIFNPYTQTK
HHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEECCCCEEECCCCCCCCCCEEEECCCHHCC
KFDPICKYIKFWIPELSKVPNKDILNGEISDLEKNNYYGAIVNHNTQQRLFKDLYAHI
CCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCEEEEEECCCHHHHHHHHHHHCC
>Mature Secondary Structure
MNNPRILFWHRKDLRIYDNNALSKAFSLSNAITSTYIFDQNYSQDFNANSRAWFLGNSLQ
CCCCCEEEEECCCCEEECCCHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCEEEECCHHH
ELSRNWETLGSRMIIDEGNPLTLIPKLAQLIDAKFVAWNKAIEPYEINRDLEIKNTLKKF
HHHHHHHHHCCEEEEECCCCEEHHHHHHHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHH
KIEIIELWDHLLLEPSQIHTGSNKPYTVYGPFYKKLKSKLQLLTSKKNLNNIGKLKDLEI
HHHHHHHHHHHHCCCHHHCCCCCCCEEEECHHHHHHHHHHHHHHHHHCCCCCCCCEEEEE
GIKESQKIDSSRLILEKFIKKVNFDGINLCPCKPGEIAAEKLLDQFINQDKIGSYASSRD
CCCHHHCCCHHHHHHHHHHHHCCCCCCEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCC
FPDQNGTSFLSASLRFGTISIRKVWNSTLFTDLKNKNNHNKISIETWQKELVWREFYQHC
CCCCCCCHHEEEEEEECEEHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHH
LFHFPELEKGPYRKKWDHFPWQNNHEWFDRWSNGETGVPIIDAAMRQLNSTGWMHNRCRM
HHCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHH
IVASFLVKDLICNWQMGEEKFMKVLVDGDLAANNGGWQWSASSGMDPKPLRIFNPYTQTK
HHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEECCCCEEECCCCCCCCCCEEEECCCHHCC
KFDPICKYIKFWIPELSKVPNKDILNGEISDLEKNNYYGAIVNHNTQQRLFKDLYAHI
CCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCEEEEEECCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2837735; 2110564; 9360600 [H]