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The map label for this gene is ligA [H]

Identifier: 123968278

GI number: 123968278

Start: 659728

End: 660477

Strand: Reverse

Name: ligA [H]

Synonym: A9601_07431

Alternate gene names: 123968278

Gene position: 660477-659728 (Counterclockwise)

Preceding gene: 123968279

Following gene: 123968277

Centisome position: 39.55

GC content: 32.0

Gene sequence:

>750_bases
ATGAAGACTTATTTAGAAGAACGAATTGAGTGGTATGACGATAATTATAGAAATGGCAATGCTTTAATCTCTGATAAGCA
GTTCGACCAACTTGAAAAAAATTTATTAAGAACAAACCCAAATTGTGATTACTTTAAAAAGAAAAATAAAATTGTTTTAC
CTTCATTAGAAAAGGATTCAATAGATGAATTTTTGAAAGGATTATTAGTAGATACCAGATTATTAATTGAACCAAAAATT
GATGGTTGTGCTGTTGCTTTGCAATATAGGGATGGAACCTTGGAGAAAGCAATTTCAAGAAAAGGGGCCGACGTTACTAG
TAAACTTATTAAAGTTCAAGACATTCCCAATAATCTCCCTTTAAGAGGGGTTCTCCAAGTTAGAGGTGAATTATATGCAC
CCAACCAAAGTCCAAATATCTCCCAGAAAATCGCTTCTGGATTTCTAAGAGCTAAAGAAGGATTTTCTGAAAGTCTTAGC
TTCTGCGCATTTCAAATACTTAATTCAACACTTAACCAATATGAGTCCAAAAAGAGTCTTTCAAAGCTTGGTTTCACGAT
CCCTCAGGATATTTCATGTAACTTTACGAGCCAAGTTGAAGTATTTAGAAAACAATGGCTAGAAGGGAAGCTTTTTAGTA
AATATCCAACAGATGGAATAGTAGTAAAAATAAATTCTAGAAAATTACAATTGATTAGAGAAAAATCAAATTTAGATTAT
CCTTATTGGCAAGTGGCAATAAAACGTTAA

Upstream 100 bases:

>100_bases
TTCATTTCTTTTATGGACTGTTAAATTTGAAGATAGAGAAAAATGGATTATAGATAAGCCAAGTTGGACACCTGACCAAA
AACTTAAGCCATCTTATTAA

Downstream 100 bases:

>100_bases
TGGAATTAATACACCAGATTTTCCCTACTTGGCATATTTACTTGGATATGTTTTTGGTTGGCTTTGCAACTTACGGTTTT
CTGAGAATTAAGAAAAAAAT

Product: NAD-dependent DNA ligase N-terminus

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] [H]

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MKTYLEERIEWYDDNYRNGNALISDKQFDQLEKNLLRTNPNCDYFKKKNKIVLPSLEKDSIDEFLKGLLVDTRLLIEPKI
DGCAVALQYRDGTLEKAISRKGADVTSKLIKVQDIPNNLPLRGVLQVRGELYAPNQSPNISQKIASGFLRAKEGFSESLS
FCAFQILNSTLNQYESKKSLSKLGFTIPQDISCNFTSQVEVFRKQWLEGKLFSKYPTDGIVVKINSRKLQLIREKSNLDY
PYWQVAIKR

Sequences:

>Translated_249_residues
MKTYLEERIEWYDDNYRNGNALISDKQFDQLEKNLLRTNPNCDYFKKKNKIVLPSLEKDSIDEFLKGLLVDTRLLIEPKI
DGCAVALQYRDGTLEKAISRKGADVTSKLIKVQDIPNNLPLRGVLQVRGELYAPNQSPNISQKIASGFLRAKEGFSESLS
FCAFQILNSTLNQYESKKSLSKLGFTIPQDISCNFTSQVEVFRKQWLEGKLFSKYPTDGIVVKINSRKLQLIREKSNLDY
PYWQVAIKR
>Mature_249_residues
MKTYLEERIEWYDDNYRNGNALISDKQFDQLEKNLLRTNPNCDYFKKKNKIVLPSLEKDSIDEFLKGLLVDTRLLIEPKI
DGCAVALQYRDGTLEKAISRKGADVTSKLIKVQDIPNNLPLRGVLQVRGELYAPNQSPNISQKIASGFLRAKEGFSESLS
FCAFQILNSTLNQYESKKSLSKLGFTIPQDISCNFTSQVEVFRKQWLEGKLFSKYPTDGIVVKINSRKLQLIREKSNLDY
PYWQVAIKR

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD-dependent DNA ligase family. LigA subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082305, Length=250, Percent_Identity=27.6, Blast_Score=72, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001357
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994 [H]

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 28656; Mature: 28656

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTYLEERIEWYDDNYRNGNALISDKQFDQLEKNLLRTNPNCDYFKKKNKIVLPSLEKDS
CCCHHHHHHHHHCCCCCCCCEEECCHHHHHHHHHHHHCCCCCHHHHCCCCEECCCCCCCH
IDEFLKGLLVDTRLLIEPKIDGCAVALQYRDGTLEKAISRKGADVTSKLIKVQDIPNNLP
HHHHHHHHHHHHHEEEECCCCCEEEEEEECCCHHHHHHHHCCCHHHHHHHHHCCCCCCCC
LRGVLQVRGELYAPNQSPNISQKIASGFLRAKEGFSESLSFCAFQILNSTLNQYESKKSL
HHHHHHHCCEEECCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHH
SKLGFTIPQDISCNFTSQVEVFRKQWLEGKLFSKYPTDGIVVKINSRKLQLIREKSNLDY
HHHCCCCCCCCCCCCHHHHHHHHHHHHCCCHHCCCCCCCEEEEECCCCEEHHHHHCCCCC
PYWQVAIKR
CEEEEEECC
>Mature Secondary Structure
MKTYLEERIEWYDDNYRNGNALISDKQFDQLEKNLLRTNPNCDYFKKKNKIVLPSLEKDS
CCCHHHHHHHHHCCCCCCCCEEECCHHHHHHHHHHHHCCCCCHHHHCCCCEECCCCCCCH
IDEFLKGLLVDTRLLIEPKIDGCAVALQYRDGTLEKAISRKGADVTSKLIKVQDIPNNLP
HHHHHHHHHHHHHEEEECCCCCEEEEEEECCCHHHHHHHHCCCHHHHHHHHHCCCCCCCC
LRGVLQVRGELYAPNQSPNISQKIASGFLRAKEGFSESLSFCAFQILNSTLNQYESKKSL
HHHHHHHCCEEECCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHH
SKLGFTIPQDISCNFTSQVEVFRKQWLEGKLFSKYPTDGIVVKINSRKLQLIREKSNLDY
HHHCCCCCCCCCCCCHHHHHHHHHHHHCCCHHCCCCCCCEEEEECCCCEEHHHHHCCCCC
PYWQVAIKR
CEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA