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The map label for this gene is gltX

Identifier: 123968065

GI number: 123968065

Start: 459758

End: 461188

Strand: Reverse

Name: gltX

Synonym: A9601_05281

Alternate gene names: 123968065

Gene position: 461188-459758 (Counterclockwise)

Preceding gene: 123968066

Following gene: 123968061

Centisome position: 27.62

GC content: 32.56

Gene sequence:

>1431_bases
TTGGAAAAACGTTTAAGACTAGCCCCGAGTCCAACGGGTTTATTTCATATTGGGACAGCGCGAACAGCACTATTCAATTG
GTTGTATGCACAAAAAATAGGTGGAAAATTTCTTATCAGAATAGAAGATACAGATTTTCTTCGATCTAAATCTGAATATA
CAAAAAATATATTACAGGGCTTGAAATGGCTTGGACTTAAATGGGATGAAGAACCTATAAAGCAAAGTGACAGAATTTCG
ATTCACAAAAGTCATATCAAAAAACTATTAGAATGTGGAGCTGCATATAGGTGCTTTACATCAGAAGATGAAATATCTGA
ATTAAGAGAAGAACAAAAAAAGAAAGGATTACCTCCAAAGCATGATAATAGACACAGAAGTCTTTCAAAAGAAGAAATAG
AAACATTCATATCCCAAGGGCGGACTTCAGTAATAAGATTCAAGATTGATGAAAAAATTGTTATTAAATGGATAGATCAG
ATAAGAGGCGAAATCAAATGGCAGGGGAAGGATTTGGGCGGTGATTTAGTTTTATCAAGAAGGGCCAAGGGATATGAGAT
TGGCGATCCTTTGTATAATCTTGCAGTTGTAGTTGATGATAATTTCATGAATATTACTCACGTCGTAAGGGGTGAAGACC
ATATCTCGAACACTGCAAAACAAATATTGATTTATAACGCATTAAATTTTAATTTGCCAACTTTTTCGCATACACCCTTA
ATACTAAATAGCGAAGGGAAAAAATTATCGAAGAGAGATTGCGTTACTTCAATCGATGAATTTAGAGAAATGGGATATTT
ACCTGAGGCCCTATCGAACTATATGGCATTTTTAGGTTGGTCTCCAAAAACTGCCGACAGAGAAATACTTTCACTTGAAG
AGATATCTGAAATTTTTGACTTATCAGAAATAAATAAAGCTGGAGCCAAATTTAGTTGGGAAAAACTCAACTGGATTAAT
TCTCAATACATAAAAAATATGGAATCAATAAAGTTAGTTGAGATCATGAGAACTTACTGGGATGAAAATGGTTGGGAACC
GCCATCTCAAGAATGGGCTAATAAGTTAGCAATTTTGATTAGAGACTCTATGACTCTTTTAAAAGATTCAATTGATCAAT
CTAAACCATTTTTCTTAATACCTACAATTCAAAAAGAAGGTCAAGATTTTCTGGAAAATCGGGAAAGCAAATTATCTTTA
AAACTAATCTTAAATTATTTAATTGAGAAAAATACTATAAAACTAAATAAAGAGAAAGCCAAAGAAATAATAAACGAAAT
CTCAAAAAAGCATAATATTAAAAAAGGGATATTAATGAAATCATTAAGAGTAGCCTTTTTTGGATCTCTCATTGGGCCAG
ATTTAATTCAAAGTTGGGAGCTTTTCGCAGAGAGTAAAACTGATAGAACTCGAATTGAAAGATGTCTTTAA

Upstream 100 bases:

>100_bases
CAAAACTCTGGGACCGTAGTTCAACTGGTTAGAGCACCGCCCTGTCACGGCGGAAGTTGCGGGTTCGAATCCCGTCGGTC
CCGTTTTTTCTAAAAGAAAT

Downstream 100 bases:

>100_bases
TCAATCAAAATTATTTTTTTGGCCTAATTTAAGTCTATTCACCAAAGGTTTAGCTGAAAGTCCTTGTATTCCTACCGTCA
TCAAAATAGTAAGAAAAACT

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MEKRLRLAPSPTGLFHIGTARTALFNWLYAQKIGGKFLIRIEDTDFLRSKSEYTKNILQGLKWLGLKWDEEPIKQSDRIS
IHKSHIKKLLECGAAYRCFTSEDEISELREEQKKKGLPPKHDNRHRSLSKEEIETFISQGRTSVIRFKIDEKIVIKWIDQ
IRGEIKWQGKDLGGDLVLSRRAKGYEIGDPLYNLAVVVDDNFMNITHVVRGEDHISNTAKQILIYNALNFNLPTFSHTPL
ILNSEGKKLSKRDCVTSIDEFREMGYLPEALSNYMAFLGWSPKTADREILSLEEISEIFDLSEINKAGAKFSWEKLNWIN
SQYIKNMESIKLVEIMRTYWDENGWEPPSQEWANKLAILIRDSMTLLKDSIDQSKPFFLIPTIQKEGQDFLENRESKLSL
KLILNYLIEKNTIKLNKEKAKEIINEISKKHNIKKGILMKSLRVAFFGSLIGPDLIQSWELFAESKTDRTRIERCL

Sequences:

>Translated_476_residues
MEKRLRLAPSPTGLFHIGTARTALFNWLYAQKIGGKFLIRIEDTDFLRSKSEYTKNILQGLKWLGLKWDEEPIKQSDRIS
IHKSHIKKLLECGAAYRCFTSEDEISELREEQKKKGLPPKHDNRHRSLSKEEIETFISQGRTSVIRFKIDEKIVIKWIDQ
IRGEIKWQGKDLGGDLVLSRRAKGYEIGDPLYNLAVVVDDNFMNITHVVRGEDHISNTAKQILIYNALNFNLPTFSHTPL
ILNSEGKKLSKRDCVTSIDEFREMGYLPEALSNYMAFLGWSPKTADREILSLEEISEIFDLSEINKAGAKFSWEKLNWIN
SQYIKNMESIKLVEIMRTYWDENGWEPPSQEWANKLAILIRDSMTLLKDSIDQSKPFFLIPTIQKEGQDFLENRESKLSL
KLILNYLIEKNTIKLNKEKAKEIINEISKKHNIKKGILMKSLRVAFFGSLIGPDLIQSWELFAESKTDRTRIERCL
>Mature_476_residues
MEKRLRLAPSPTGLFHIGTARTALFNWLYAQKIGGKFLIRIEDTDFLRSKSEYTKNILQGLKWLGLKWDEEPIKQSDRIS
IHKSHIKKLLECGAAYRCFTSEDEISELREEQKKKGLPPKHDNRHRSLSKEEIETFISQGRTSVIRFKIDEKIVIKWIDQ
IRGEIKWQGKDLGGDLVLSRRAKGYEIGDPLYNLAVVVDDNFMNITHVVRGEDHISNTAKQILIYNALNFNLPTFSHTPL
ILNSEGKKLSKRDCVTSIDEFREMGYLPEALSNYMAFLGWSPKTADREILSLEEISEIFDLSEINKAGAKFSWEKLNWIN
SQYIKNMESIKLVEIMRTYWDENGWEPPSQEWANKLAILIRDSMTLLKDSIDQSKPFFLIPTIQKEGQDFLENRESKLSL
KLILNYLIEKNTIKLNKEKAKEIINEISKKHNIKKGILMKSLRVAFFGSLIGPDLIQSWELFAESKTDRTRIERCL

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=494, Percent_Identity=28.9473684210526, Blast_Score=211, Evalue=1e-54,
Organism=Escherichia coli, GI1788743, Length=463, Percent_Identity=35.2051835853132, Blast_Score=276, Evalue=2e-75,
Organism=Escherichia coli, GI226510925, Length=253, Percent_Identity=32.8063241106719, Blast_Score=126, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI71993394, Length=479, Percent_Identity=31.3152400835073, Blast_Score=207, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6324540, Length=333, Percent_Identity=39.6396396396396, Blast_Score=231, Evalue=1e-61,
Organism=Saccharomyces cerevisiae, GI6321192, Length=229, Percent_Identity=27.0742358078603, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24665321, Length=486, Percent_Identity=33.3333333333333, Blast_Score=237, Evalue=1e-62,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_PROMS (A2BPV5)

Other databases:

- EMBL:   CP000551
- RefSeq:   YP_001008923.1
- ProteinModelPortal:   A2BPV5
- SMR:   A2BPV5
- STRING:   A2BPV5
- GeneID:   4717226
- GenomeReviews:   CP000551_GR
- KEGG:   pmb:A9601_05281
- eggNOG:   COG0008
- HOGENOM:   HBG628189
- OMA:   YRCFTSE
- PhylomeDB:   A2BPV5
- ProtClustDB:   PRK01406
- BioCyc:   PMAR146891:A9601_05281-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 55285; Mature: 55285

Theoretical pI: Translated: 9.26; Mature: 9.26

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 251-251

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKRLRLAPSPTGLFHIGTARTALFNWLYAQKIGGKFLIRIEDTDFLRSKSEYTKNILQG
CCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHH
LKWLGLKWDEEPIKQSDRISIHKSHIKKLLECGAAYRCFTSEDEISELREEQKKKGLPPK
HHHCCCCCCHHHCCCCCCCHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHCCCCCC
HDNRHRSLSKEEIETFISQGRTSVIRFKIDEKIVIKWIDQIRGEIKWQGKDLGGDLVLSR
CCCHHHCCCHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCCEEEECCCCCCCHHEEEC
RAKGYEIGDPLYNLAVVVDDNFMNITHVVRGEDHISNTAKQILIYNALNFNLPTFSHTPL
CCCCCCCCCCCEEEEEEEECCCEEEHHEECCCHHHHHHHHHEEEEEEEECCCCCCCCCCE
ILNSEGKKLSKRDCVTSIDEFREMGYLPEALSNYMAFLGWSPKTADREILSLEEISEIFD
EECCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
LSEINKAGAKFSWEKLNWINSQYIKNMESIKLVEIMRTYWDENGWEPPSQEWANKLAILI
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
RDSMTLLKDSIDQSKPFFLIPTIQKEGQDFLENRESKLSLKLILNYLIEKNTIKLNKEKA
HHHHHHHHHHHCCCCCEEEEECHHHCCHHHHHCHHHHHHHHHHHHHHHCCCCEEECHHHH
KEIINEISKKHNIKKGILMKSLRVAFFGSLIGPDLIQSWELFAESKTDRTRIERCL
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCHHHHHHHCC
>Mature Secondary Structure
MEKRLRLAPSPTGLFHIGTARTALFNWLYAQKIGGKFLIRIEDTDFLRSKSEYTKNILQG
CCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHH
LKWLGLKWDEEPIKQSDRISIHKSHIKKLLECGAAYRCFTSEDEISELREEQKKKGLPPK
HHHCCCCCCHHHCCCCCCCHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHCCCCCC
HDNRHRSLSKEEIETFISQGRTSVIRFKIDEKIVIKWIDQIRGEIKWQGKDLGGDLVLSR
CCCHHHCCCHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCCEEEECCCCCCCHHEEEC
RAKGYEIGDPLYNLAVVVDDNFMNITHVVRGEDHISNTAKQILIYNALNFNLPTFSHTPL
CCCCCCCCCCCEEEEEEEECCCEEEHHEECCCHHHHHHHHHEEEEEEEECCCCCCCCCCE
ILNSEGKKLSKRDCVTSIDEFREMGYLPEALSNYMAFLGWSPKTADREILSLEEISEIFD
EECCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
LSEINKAGAKFSWEKLNWINSQYIKNMESIKLVEIMRTYWDENGWEPPSQEWANKLAILI
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
RDSMTLLKDSIDQSKPFFLIPTIQKEGQDFLENRESKLSLKLILNYLIEKNTIKLNKEKA
HHHHHHHHHHHCCCCCEEEEECHHHCCHHHHHCHHHHHHHHHHHHHHHCCCCEEECHHHH
KEIINEISKKHNIKKGILMKSLRVAFFGSLIGPDLIQSWELFAESKTDRTRIERCL
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA