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The map label for this gene is mhpA [H]

Identifier: 121610354

GI number: 121610354

Start: 3793473

End: 3795125

Strand: Direct

Name: mhpA [H]

Synonym: Veis_3422

Alternate gene names: 121610354

Gene position: 3793473-3795125 (Clockwise)

Preceding gene: 121610353

Following gene: 121610355

Centisome position: 68.15

GC content: 70.78

Gene sequence:

>1653_bases
ATGGAAACCGATACCTTTGCCCCGGCCTATGAGCTGCCGAGCTGGCCCTTCGAGTCGCCGCCCGAACTCGGTGGCAGCGC
CATTCGACGCCACCCGATCGTGATCGTCGGCGCGGGCCTGGCCGGCCTCACGCTGGCCTGCGATCTCGCGCAGCGCGGCG
TGCGGGCGGTGCTGCTCGACGAGGACGACACCGTGGGCGTGCGCGGCGCCTCCTCGCGCGGCATTTGCTACGCGCAAAAG
AGCCTGGAGATCTTCGAGCGTCTGGGCCTGTACCGGCGCGTCGCGGACAAGGGCATCACCTGGTCCTTCGGCCGCACCTT
CTCGGGGGAGACCGAGGTCTACAGCTTCAACCTGCAGACCGGCAGCGTCTCGGCGCAGCCGCCCTTCATCAACCTGCAGC
AGTTCTACATCGAGTGGTTCCTGGTCGACCGCATCCTCGAACTCGGCCTCACCGATGTGCGCTGGAAGAACCGCGCAACG
CATGTGGAACCCCTGGCCGACGGCGTGCGCCTGGAGATCGAAACCCCGGCCGGCGCCTACACGATGGAAACGGACTACCT
CATCGACGCCACCGGCGCGAGCAGCCCGATTCGCACACAGCTCGGCCTCGAGGCGCACGCCTCGCGCAGCACCGACCGCT
GGTGCATCAGCGACGTGCGCTTCAAGAAGCCGCTCCCGGTGGAGCGCTGGACCTGGGTGGATGCGCCCTTCAACGACGGT
CGCGCCGTCTGGCAGCACCTGATGGCCGACGGTGTGTGGCGCATCGACTACCAGATGCCCGAGGACTGCGACACCCGGCA
GATCAGCCGCCCGGAGGTGGCGGGCGCGCGTCTGCGCGAGCAGCTCGGCCCCGATGTCGAATTCGAATTCGTCTGGATCG
GCCCCTACGGCTACCGCGACCACCTGCTGGAACGCTTCCGGCACGGGCGCCTGTTCTTCATCGGCGACTCTGCCCATGTG
GTCAGCCCCTTCGGCGCGCGCGGTGGCAATACCGGCATCCAGGACGCCGGCAACCTCGGCTGGAAGCTCGCCCTGGTGCT
GCAGGGCCACGCCGGCGACGCGCTGCTGGACACCTACGATGCCGAACGCCGCGCCGCAGCCGTGCAGAACCTCGAGGTGA
CGAGCCGCTCGGCGCGCTTTCTGGCGCCGCGCTCGCCGGCCGAGCACAGGCTGCGCCGCGCCGTGGTCGCATTGGCGGCG
CGCCATCCGTTCGCGCGCGCCCTCGTCAACACCGGCCGCATGTCGGTGGCCAATGATTACCCGGCGGCGCCACACCTGCC
CGAAGGCGGCCGCAGCGTGCAGAACCTGCCGCTGCGCCGGGCCGATGGCACGGCAACGACGCTGATGCAACTGCTGGCCG
AGGGCACGCAATGCCTGGGCCTGTGGTTCGCACCGTCCCCGGAGCAGGCGCGCGCCGCGCTCGATACCCTGGCGGCGCGG
CCGCTGCGCCTGCTGGCCGTCGGCGGCGACGGCACCCTGCCCACATTGCAGGCCGACGAAACCCTCAGCCGCCAGCTCGG
CGCCGGCGCACCGGGCAGCTTCGTGCTGGTGCGCCCCGACGCTTACCGCGCCGCCGTGCTGGCCAACGCCACACCGGCCG
CCATCGCCACCACGCTGCGCACCGCCCTGGCCCGGGAAGGAGACCGCCCATGA

Upstream 100 bases:

>100_bases
GGTGGGTGGAACAACGAACTGCCGATGCCATGGCCGCGTGGATGCGCGCGCTCTGAGCGCCACCGAATCCCCGAAAACCC
GAACCTTGGAGACAAATTGG

Downstream 100 bases:

>100_bases
TCACCGAACCCCGCATCCCCGATCCCGACGGCTTCTACGCCGCCTGGCTCAGCGCCCACGAGGGCCTCGACGAGGCCCGC
AGCGCCGACCTCGATGCCCG

Product: FAD-binding monooxygenase

Products: H2O; 3-(2,3-dihydroxyphenyl)propionate; NAD [C]

Alternate protein names: 3-HCI hydroxylase; 3-HPP hydroxylase; Flavin-type monooxygenase [H]

Number of amino acids: Translated: 550; Mature: 550

Protein sequence:

>550_residues
METDTFAPAYELPSWPFESPPELGGSAIRRHPIVIVGAGLAGLTLACDLAQRGVRAVLLDEDDTVGVRGASSRGICYAQK
SLEIFERLGLYRRVADKGITWSFGRTFSGETEVYSFNLQTGSVSAQPPFINLQQFYIEWFLVDRILELGLTDVRWKNRAT
HVEPLADGVRLEIETPAGAYTMETDYLIDATGASSPIRTQLGLEAHASRSTDRWCISDVRFKKPLPVERWTWVDAPFNDG
RAVWQHLMADGVWRIDYQMPEDCDTRQISRPEVAGARLREQLGPDVEFEFVWIGPYGYRDHLLERFRHGRLFFIGDSAHV
VSPFGARGGNTGIQDAGNLGWKLALVLQGHAGDALLDTYDAERRAAAVQNLEVTSRSARFLAPRSPAEHRLRRAVVALAA
RHPFARALVNTGRMSVANDYPAAPHLPEGGRSVQNLPLRRADGTATTLMQLLAEGTQCLGLWFAPSPEQARAALDTLAAR
PLRLLAVGGDGTLPTLQADETLSRQLGAGAPGSFVLVRPDAYRAAVLANATPAAIATTLRTALAREGDRP

Sequences:

>Translated_550_residues
METDTFAPAYELPSWPFESPPELGGSAIRRHPIVIVGAGLAGLTLACDLAQRGVRAVLLDEDDTVGVRGASSRGICYAQK
SLEIFERLGLYRRVADKGITWSFGRTFSGETEVYSFNLQTGSVSAQPPFINLQQFYIEWFLVDRILELGLTDVRWKNRAT
HVEPLADGVRLEIETPAGAYTMETDYLIDATGASSPIRTQLGLEAHASRSTDRWCISDVRFKKPLPVERWTWVDAPFNDG
RAVWQHLMADGVWRIDYQMPEDCDTRQISRPEVAGARLREQLGPDVEFEFVWIGPYGYRDHLLERFRHGRLFFIGDSAHV
VSPFGARGGNTGIQDAGNLGWKLALVLQGHAGDALLDTYDAERRAAAVQNLEVTSRSARFLAPRSPAEHRLRRAVVALAA
RHPFARALVNTGRMSVANDYPAAPHLPEGGRSVQNLPLRRADGTATTLMQLLAEGTQCLGLWFAPSPEQARAALDTLAAR
PLRLLAVGGDGTLPTLQADETLSRQLGAGAPGSFVLVRPDAYRAAVLANATPAAIATTLRTALAREGDRP
>Mature_550_residues
METDTFAPAYELPSWPFESPPELGGSAIRRHPIVIVGAGLAGLTLACDLAQRGVRAVLLDEDDTVGVRGASSRGICYAQK
SLEIFERLGLYRRVADKGITWSFGRTFSGETEVYSFNLQTGSVSAQPPFINLQQFYIEWFLVDRILELGLTDVRWKNRAT
HVEPLADGVRLEIETPAGAYTMETDYLIDATGASSPIRTQLGLEAHASRSTDRWCISDVRFKKPLPVERWTWVDAPFNDG
RAVWQHLMADGVWRIDYQMPEDCDTRQISRPEVAGARLREQLGPDVEFEFVWIGPYGYRDHLLERFRHGRLFFIGDSAHV
VSPFGARGGNTGIQDAGNLGWKLALVLQGHAGDALLDTYDAERRAAAVQNLEVTSRSARFLAPRSPAEHRLRRAVVALAA
RHPFARALVNTGRMSVANDYPAAPHLPEGGRSVQNLPLRRADGTATTLMQLLAEGTQCLGLWFAPSPEQARAALDTLAAR
PLRLLAVGGDGTLPTLQADETLSRQLGAGAPGSFVLVRPDAYRAAVLANATPAAIATTLRTALAREGDRP

Specific function: Catalyzes the insertion of one atom of molecular oxygen into position 2 of the phenyl ring of 3-(3- hydroxyphenyl)propionate (3-HPP) and hydroxycinnamic acid (3HCI) [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pheA/tfdB FAD monooxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786543, Length=527, Percent_Identity=22.9601518026565, Blast_Score=100, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.- [C]

Molecular weight: Translated: 60269; Mature: 60269

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METDTFAPAYELPSWPFESPPELGGSAIRRHPIVIVGAGLAGLTLACDLAQRGVRAVLLD
CCCCCCCCCCCCCCCCCCCCHHCCCHHHHHCCEEEEECCHHHHHHHHHHHHCCCEEEEEC
EDDTVGVRGASSRGICYAQKSLEIFERLGLYRRVADKGITWSFGRTFSGETEVYSFNLQT
CCCCCEEECCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCEEEEEEEEC
GSVSAQPPFINLQQFYIEWFLVDRILELGLTDVRWKNRATHVEPLADGVRLEIETPAGAY
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCE
TMETDYLIDATGASSPIRTQLGLEAHASRSTDRWCISDVRFKKPLPVERWTWVDAPFNDG
EEECCEEEECCCCCCCCHHHCCCHHHCCCCCCCEECCCCCCCCCCCCCCEEEECCCCCCC
RAVWQHLMADGVWRIDYQMPEDCDTRQISRPEVAGARLREQLGPDVEFEFVWIGPYGYRD
HHHHHHHHHCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCHHH
HLLERFRHGRLFFIGDSAHVVSPFGARGGNTGIQDAGNLGWKLALVLQGHAGDALLDTYD
HHHHHHHCCCEEEEECCCCEECCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHC
AERRAAAVQNLEVTSRSARFLAPRSPAEHRLRRAVVALAARHPFARALVNTGRMSVANDY
HHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCEEECCCC
PAAPHLPEGGRSVQNLPLRRADGTATTLMQLLAEGTQCLGLWFAPSPEQARAALDTLAAR
CCCCCCCCCCCHHHCCCCCCCCCHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHCC
PLRLLAVGGDGTLPTLQADETLSRQLGAGAPGSFVLVRPDAYRAAVLANATPAAIATTLR
CEEEEEECCCCCCCCCCCHHHHHHHHCCCCCCCEEEECCCCCEEEEECCCCHHHHHHHHH
TALAREGDRP
HHHHHCCCCC
>Mature Secondary Structure
METDTFAPAYELPSWPFESPPELGGSAIRRHPIVIVGAGLAGLTLACDLAQRGVRAVLLD
CCCCCCCCCCCCCCCCCCCCHHCCCHHHHHCCEEEEECCHHHHHHHHHHHHCCCEEEEEC
EDDTVGVRGASSRGICYAQKSLEIFERLGLYRRVADKGITWSFGRTFSGETEVYSFNLQT
CCCCCEEECCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCEEEEEEEEC
GSVSAQPPFINLQQFYIEWFLVDRILELGLTDVRWKNRATHVEPLADGVRLEIETPAGAY
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCE
TMETDYLIDATGASSPIRTQLGLEAHASRSTDRWCISDVRFKKPLPVERWTWVDAPFNDG
EEECCEEEECCCCCCCCHHHCCCHHHCCCCCCCEECCCCCCCCCCCCCCEEEECCCCCCC
RAVWQHLMADGVWRIDYQMPEDCDTRQISRPEVAGARLREQLGPDVEFEFVWIGPYGYRD
HHHHHHHHHCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCHHH
HLLERFRHGRLFFIGDSAHVVSPFGARGGNTGIQDAGNLGWKLALVLQGHAGDALLDTYD
HHHHHHHCCCEEEEECCCCEECCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHC
AERRAAAVQNLEVTSRSARFLAPRSPAEHRLRRAVVALAARHPFARALVNTGRMSVANDY
HHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCEEECCCC
PAAPHLPEGGRSVQNLPLRRADGTATTLMQLLAEGTQCLGLWFAPSPEQARAALDTLAAR
CCCCCCCCCCCHHHCCCCCCCCCHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHCC
PLRLLAVGGDGTLPTLQADETLSRQLGAGAPGSFVLVRPDAYRAAVLANATPAAIATTLR
CEEEEEECCCCCCCCCCCHHHHHHHHCCCCCCCEEEECCCCCEEEEECCCCHHHHHHHHH
TALAREGDRP
HHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-(3-hydroxyphenyl)propionate; NADH; O2 [C]

Specific reaction: 3-(3-hydroxyphenyl)propionate + NADH + O2 = H2O + 3-(2,3-dihydroxyphenyl)propionate + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA