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The map label for this gene is purR [H]

Identifier: 121610277

GI number: 121610277

Start: 3721381

End: 3722406

Strand: Direct

Name: purR [H]

Synonym: Veis_3336

Alternate gene names: 121610277

Gene position: 3721381-3722406 (Clockwise)

Preceding gene: 121610272

Following gene: 121610278

Centisome position: 66.85

GC content: 58.67

Gene sequence:

>1026_bases
ATGCGAAATCCAAATTCGACTCACCCCGTCACGATCCTTGACGTCGCCGCAGAGGCAAATCTTTCCAAGTCCACTGTTTC
GCTCGTGCTGAAGGGCAGTCCATTGATTCCGGCGGAAACCGCCGACCGGGTCAGGGCGGCTGCCAGCAAGCTCGGATACG
TCTACAACAGCCGGGCCGGCGAGTTGCGTGATGGCACTTCCAAGACCATTGGTGTCGTCGTCAACGATCTTGCCAATCCG
TTCTTCGCTGAAATCCTCGTCGGCGTCGAGCGGAAACTCGTCGAGGCCGGGTACATCGTGTTGATGGCGCACACGAATGA
AGACGTTGATCGGCAGGAACAAGTCTTGCAAACCATGAGGGAGCAAGGTGCTGCCGGTATCGTGCTTTGTCCCGCGTACG
GAACACCCCGCACGCTCACGAAGGAAGTGGAAGCCTGGGGCATCCCTTTGGTGGTGATGGTTCGGACACTGGGCCCGGGC
AAGTACGACTTTGCTGGTGGCGACAACGAGCGCGGAATCTATGTGGCGACCAAGCATCTACTTGACGCGGGACACGAGCG
CATCGGTTTTCTCGGTGGATTGGCGGGCGTTGTGCTTGAACAGCGACTGAACGGTTTCAGGACGGCGCTTGCGGAGCGAA
AGCTGAGGTTCGACGAAGAACTGGTGGTGCGGGCGAAACCGACCCGGCAAAGCGGCTATGAGACGATGTGTGCGTTGCTC
GACATAAAACCTCGGCCCACCGCGGCTGTTTGCTACAACGACGTTGTGGCTTTCGGCGCAATGTCTGCGTTGGGAGAGCG
AGGGATGCACGCCGGCCGGGACTTCGCTTTGACTGGATTCGACAACGTTCAAGACGCAGCACATTCGAATCCGCCCTTGA
CCACGGTGGACATCCGTCCGGGCGCATTGGGTGAGCAAGCGGCCTCGATTTTGTTGAGCCGCATTCAGACGCCAGGCCAA
GCACGCCAAATGTTTTTGGCAAAACCACAACTGGTCGTGCGAAAGTCGACGGGCGCCGTTGCATAA

Upstream 100 bases:

>100_bases
CGAAAATAGGGTAAACACTGAGTCAGCCCTTCGCTGCCGAGTAAGCACGCCAACGGATGTGAAGCATCTTGCGTAAAGTG
CGGGGGCTGTTTAGACCATC

Downstream 100 bases:

>100_bases
CCCGAATCCAGGGCGAACCTCATCGGCGAGCGGTTCGGGGTGCAATACAGCGTGGGCCATTTGTGGCGCCTGCTACGTCG
GCTGGGCTTTTCCTGCCAGA

Product: periplasmic-binding protein/LacI transcriptional regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MRNPNSTHPVTILDVAAEANLSKSTVSLVLKGSPLIPAETADRVRAAASKLGYVYNSRAGELRDGTSKTIGVVVNDLANP
FFAEILVGVERKLVEAGYIVLMAHTNEDVDRQEQVLQTMREQGAAGIVLCPAYGTPRTLTKEVEAWGIPLVVMVRTLGPG
KYDFAGGDNERGIYVATKHLLDAGHERIGFLGGLAGVVLEQRLNGFRTALAERKLRFDEELVVRAKPTRQSGYETMCALL
DIKPRPTAAVCYNDVVAFGAMSALGERGMHAGRDFALTGFDNVQDAAHSNPPLTTVDIRPGALGEQAASILLSRIQTPGQ
ARQMFLAKPQLVVRKSTGAVA

Sequences:

>Translated_341_residues
MRNPNSTHPVTILDVAAEANLSKSTVSLVLKGSPLIPAETADRVRAAASKLGYVYNSRAGELRDGTSKTIGVVVNDLANP
FFAEILVGVERKLVEAGYIVLMAHTNEDVDRQEQVLQTMREQGAAGIVLCPAYGTPRTLTKEVEAWGIPLVVMVRTLGPG
KYDFAGGDNERGIYVATKHLLDAGHERIGFLGGLAGVVLEQRLNGFRTALAERKLRFDEELVVRAKPTRQSGYETMCALL
DIKPRPTAAVCYNDVVAFGAMSALGERGMHAGRDFALTGFDNVQDAAHSNPPLTTVDIRPGALGEQAASILLSRIQTPGQ
ARQMFLAKPQLVVRKSTGAVA
>Mature_341_residues
MRNPNSTHPVTILDVAAEANLSKSTVSLVLKGSPLIPAETADRVRAAASKLGYVYNSRAGELRDGTSKTIGVVVNDLANP
FFAEILVGVERKLVEAGYIVLMAHTNEDVDRQEQVLQTMREQGAAGIVLCPAYGTPRTLTKEVEAWGIPLVVMVRTLGPG
KYDFAGGDNERGIYVATKHLLDAGHERIGFLGGLAGVVLEQRLNGFRTALAERKLRFDEELVVRAKPTRQSGYETMCALL
DIKPRPTAAVCYNDVVAFGAMSALGERGMHAGRDFALTGFDNVQDAAHSNPPLTTVDIRPGALGEQAASILLSRIQTPGQ
ARQMFLAKPQLVVRKSTGAVA

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787948, Length=331, Percent_Identity=33.8368580060423, Blast_Score=169, Evalue=3e-43,
Organism=Escherichia coli, GI1787906, Length=297, Percent_Identity=34.3434343434343, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI1790369, Length=333, Percent_Identity=29.4294294294294, Blast_Score=145, Evalue=3e-36,
Organism=Escherichia coli, GI1790194, Length=329, Percent_Identity=30.6990881458967, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI1789068, Length=291, Percent_Identity=31.2714776632302, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1789202, Length=293, Percent_Identity=33.4470989761092, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI1788474, Length=331, Percent_Identity=29.3051359516616, Blast_Score=128, Evalue=7e-31,
Organism=Escherichia coli, GI1790715, Length=318, Percent_Identity=27.3584905660377, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1786540, Length=336, Percent_Identity=28.5714285714286, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI48994940, Length=305, Percent_Identity=28.1967213114754, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1787580, Length=312, Percent_Identity=24.3589743589744, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1789456, Length=341, Percent_Identity=24.633431085044, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1786268, Length=315, Percent_Identity=21.2698412698413, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1790689, Length=325, Percent_Identity=25.8461538461538, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36602; Mature: 36602

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: PS50932 HTH_LACI_2 ; PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNPNSTHPVTILDVAAEANLSKSTVSLVLKGSPLIPAETADRVRAAASKLGYVYNSRAG
CCCCCCCCCEEEEEECCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCC
ELRDGTSKTIGVVVNDLANPFFAEILVGVERKLVEAGYIVLMAHTNEDVDRQEQVLQTMR
CCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHH
EQGAAGIVLCPAYGTPRTLTKEVEAWGIPLVVMVRTLGPGKYDFAGGDNERGIYVATKHL
HCCCCEEEEECCCCCCHHHHHHHHHHCCCHHHHEECCCCCCEECCCCCCCCCEEEEHHHH
LDAGHERIGFLGGLAGVVLEQRLNGFRTALAERKLRFDEELVVRAKPTRQSGYETMCALL
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHH
DIKPRPTAAVCYNDVVAFGAMSALGERGMHAGRDFALTGFDNVQDAAHSNPPLTTVDIRP
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHCCCCCEEEEECCC
GALGEQAASILLSRIQTPGQARQMFLAKPQLVVRKSTGAVA
CCCHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEECCCCCC
>Mature Secondary Structure
MRNPNSTHPVTILDVAAEANLSKSTVSLVLKGSPLIPAETADRVRAAASKLGYVYNSRAG
CCCCCCCCCEEEEEECCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCC
ELRDGTSKTIGVVVNDLANPFFAEILVGVERKLVEAGYIVLMAHTNEDVDRQEQVLQTMR
CCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHH
EQGAAGIVLCPAYGTPRTLTKEVEAWGIPLVVMVRTLGPGKYDFAGGDNERGIYVATKHL
HCCCCEEEEECCCCCCHHHHHHHHHHCCCHHHHEECCCCCCEECCCCCCCCCEEEEHHHH
LDAGHERIGFLGGLAGVVLEQRLNGFRTALAERKLRFDEELVVRAKPTRQSGYETMCALL
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHH
DIKPRPTAAVCYNDVVAFGAMSALGERGMHAGRDFALTGFDNVQDAAHSNPPLTTVDIRP
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHCCCCCEEEEECCC
GALGEQAASILLSRIQTPGQARQMFLAKPQLVVRKSTGAVA
CCCHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA