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The map label for this gene is rbfE [H]

Identifier: 121639698

GI number: 121639698

Start: 4199932

End: 4200753

Strand: Direct

Name: rbfE [H]

Synonym: BCG_3843

Alternate gene names: 121639698

Gene position: 4199932-4200753 (Clockwise)

Preceding gene: 121639697

Following gene: 121639699

Centisome position: 96.01

GC content: 61.44

Gene sequence:

>822_bases
GTGTCGGATCCGCACCATCCCCATATCCAGACGCACAACGCGTGGGTGGAGTTCCCTATCTTCGACGCCAAGTCACGTTC
GCTGAAGAAGGCGGTCCTGGGTAAAGCGGGCGGCACCATCGGGCGCAACAACTCCAACGTCGTCGTCATCGAAGCGTTGC
GCGACATCACCATGGAGCTGAACCTGGGTGACCGGGTCGGTCTGGTCGGACACAACGGAGCCGGCAAATCGACGCTGCTA
CGCCTGCTTTCGGGCATCTACGAGCCCACCCGCGGCTGGGCGAAGGTCACCGGAAGGGTGGCGCCGGTCTTCGATCTGGG
CATCGGCATGGACCCCGAGATCTCCGGCTACGAGAACATCATCATTCGTGGGCTGTTTCTGGGACAGACCCGCAAACAGA
TGCAGGCGAAAGTGGATGAGATCGCCGAATTCACCGAATTGGGCGAGTACCTTTCGATGCCGCTGCGCACCTATTCCACC
GGGATGCGAGTCCGCCTGGCGATGGGCGTGGTCACCAGCATCGACCCAGAGATCCTGTTGCTCGACGAAGGCATCGGCGC
CGTGGACGCCGACTTCCTGAGGAAGGCCCAGTCCCGGCTGCAGAATTTGGTCGAACGTTCCGGGATCCTGGTTTTCGCAA
GCCATTCCAACGAGTTTTTGGCTCGACTATGCAAGACCGCGATATGGATTGACCATGGCGTCATCAGGCTCGCCGGTGGT
ATCGAAGAGGTGGTACGGGCCTACGAGGGTGAGGACGCCGCCCGGCACGTGCGCGAAGTACTGGCCGAGACCCAGGCCGA
CAGACAGAACGTCCAGGGATGA

Upstream 100 bases:

>100_bases
GGCGCGCGCGCAGCTGCAACAAATGCGCCAGGGTGCGCTGCCGCCCGGGGTCGGCAAGTCGGGCCAGCACGGCCACGGCA
CCGGACAATACCTGTAAGCC

Downstream 100 bases:

>100_bases
CTGAATCGGTCTTCGCCGTTGTGGTAACCCACCGGCGCCCCGACGAGCTGGCCAAGTCGCTGGATGTGCTGACCGCCCAG
ACCCGGTTACCGGACCACCT

Product: putative O-antigen/lipopolysaccharide transport ATP-binding protein ABC transporter rfbE

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 272

Protein sequence:

>273_residues
MSDPHHPHIQTHNAWVEFPIFDAKSRSLKKAVLGKAGGTIGRNNSNVVVIEALRDITMELNLGDRVGLVGHNGAGKSTLL
RLLSGIYEPTRGWAKVTGRVAPVFDLGIGMDPEISGYENIIIRGLFLGQTRKQMQAKVDEIAEFTELGEYLSMPLRTYST
GMRVRLAMGVVTSIDPEILLLDEGIGAVDADFLRKAQSRLQNLVERSGILVFASHSNEFLARLCKTAIWIDHGVIRLAGG
IEEVVRAYEGEDAARHVREVLAETQADRQNVQG

Sequences:

>Translated_273_residues
MSDPHHPHIQTHNAWVEFPIFDAKSRSLKKAVLGKAGGTIGRNNSNVVVIEALRDITMELNLGDRVGLVGHNGAGKSTLL
RLLSGIYEPTRGWAKVTGRVAPVFDLGIGMDPEISGYENIIIRGLFLGQTRKQMQAKVDEIAEFTELGEYLSMPLRTYST
GMRVRLAMGVVTSIDPEILLLDEGIGAVDADFLRKAQSRLQNLVERSGILVFASHSNEFLARLCKTAIWIDHGVIRLAGG
IEEVVRAYEGEDAARHVREVLAETQADRQNVQG
>Mature_272_residues
SDPHHPHIQTHNAWVEFPIFDAKSRSLKKAVLGKAGGTIGRNNSNVVVIEALRDITMELNLGDRVGLVGHNGAGKSTLLR
LLSGIYEPTRGWAKVTGRVAPVFDLGIGMDPEISGYENIIIRGLFLGQTRKQMQAKVDEIAEFTELGEYLSMPLRTYSTG
MRVRLAMGVVTSIDPEILLLDEGIGAVDADFLRKAQSRLQNLVERSGILVFASHSNEFLARLCKTAIWIDHGVIRLAGGI
EEVVRAYEGEDAARHVREVLAETQADRQNVQG

Specific function: May form an ATP-driven O-antigen export apparatus, in association with RfbD [H]

COG id: COG1134

COG function: function code GM; ABC-type polysaccharide/polyol phosphate transport system, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI153792144, Length=222, Percent_Identity=24.7747747747748, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI31657092, Length=233, Percent_Identity=25.3218884120172, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1788761, Length=240, Percent_Identity=27.0833333333333, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1790467, Length=197, Percent_Identity=26.3959390862944, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI87081791, Length=179, Percent_Identity=27.9329608938547, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI87081782, Length=214, Percent_Identity=28.9719626168224, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87081709, Length=217, Percent_Identity=24.4239631336406, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87082267, Length=206, Percent_Identity=28.6407766990291, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1786319, Length=200, Percent_Identity=26, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1788450, Length=202, Percent_Identity=26.7326732673267, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI48994943, Length=178, Percent_Identity=29.2134831460674, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787089, Length=207, Percent_Identity=25.6038647342995, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI48995001, Length=182, Percent_Identity=26.9230769230769, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787758, Length=177, Percent_Identity=29.3785310734463, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1789751, Length=172, Percent_Identity=30.2325581395349, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1787712, Length=177, Percent_Identity=26.5536723163842, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1788225, Length=206, Percent_Identity=26.2135922330097, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1787041, Length=243, Percent_Identity=25.1028806584362, Blast_Score=61, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI17565586, Length=213, Percent_Identity=28.169014084507, Blast_Score=77, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI115533608, Length=210, Percent_Identity=28.0952380952381, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI28573571, Length=206, Percent_Identity=26.6990291262136, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI28573573, Length=206, Percent_Identity=26.6990291262136, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: 3.6.3.25 [C]

Molecular weight: Translated: 29993; Mature: 29862

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDPHHPHIQTHNAWVEFPIFDAKSRSLKKAVLGKAGGTIGRNNSNVVVIEALRDITMEL
CCCCCCCCEECCCCEEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCEEEEEEHHCCEEEE
NLGDRVGLVGHNGAGKSTLLRLLSGIYEPTRGWAKVTGRVAPVFDLGIGMDPEISGYENI
CCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCHHHHCCCCCCCEECCCCCCCCCCCCHHE
IIRGLFLGQTRKQMQAKVDEIAEFTELGEYLSMPLRTYSTGMRVRLAMGVVTSIDPEILL
EEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCEEEEEHHHHHCCCCCEEE
LDEGIGAVDADFLRKAQSRLQNLVERSGILVFASHSNEFLARLCKTAIWIDHGVIRLAGG
EECCCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHCCHHHHHCC
IEEVVRAYEGEDAARHVREVLAETQADRQNVQG
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SDPHHPHIQTHNAWVEFPIFDAKSRSLKKAVLGKAGGTIGRNNSNVVVIEALRDITMEL
CCCCCCCEECCCCEEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCEEEEEEHHCCEEEE
NLGDRVGLVGHNGAGKSTLLRLLSGIYEPTRGWAKVTGRVAPVFDLGIGMDPEISGYENI
CCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCHHHHCCCCCCCEECCCCCCCCCCCCHHE
IIRGLFLGQTRKQMQAKVDEIAEFTELGEYLSMPLRTYSTGMRVRLAMGVVTSIDPEILL
EEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCEEEEEHHHHHCCCCCEEE
LDEGIGAVDADFLRKAQSRLQNLVERSGILVFASHSNEFLARLCKTAIWIDHGVIRLAGG
EECCCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHCCHHHHHCC
IEEVVRAYEGEDAARHVREVLAETQADRQNVQG
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7692217 [H]