| Definition | Mycobacterium bovis BCG str. Pasteur 1173P2, complete genome. |
|---|---|
| Accession | NC_008769 |
| Length | 4,374,522 |
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The map label for this gene is leuA [H]
Identifier: 121639626
GI number: 121639626
Start: 4126856
End: 4129360
Strand: Direct
Name: leuA [H]
Synonym: BCG_3770
Alternate gene names: 121639626
Gene position: 4126856-4129360 (Clockwise)
Preceding gene: 121639614
Following gene: 121639628
Centisome position: 94.34
GC content: 68.58
Gene sequence:
>2505_bases GTGACAACTTCTGAATCGCCCGACGCCTATACCGAGTCGTTTGGGGCCCACACCATCGTGAAACCCGCCGGCCCACCTCG CGTCGGTCAGCCCTCGTGGAATCCGCAGCGAGCCTCGTCGATGCCGGTCAACCGCTACCGGCCGTTCGCCGAGGAGGTCG AGCCCATCCGGCTGAGAAACCGCACGTGGCCTGATCGCGTCATCGATCGTGCGCCGCTGTGGTGCGCGGTCGACTTACGC GATGGCAACCAGGCGCTGATCGACCCGATGAGCCCGGCCCGCAAGCGCCGCATGTTCGACCTGCTGGTCCGGATGGGCTA CAAGGAGATTGAGGTGGGGTTCCCCTCGGCCAGCCAGACCGACTTCGACTTCGTCAGAGAGATCATCGAGCAGGGCGCCA TTCCCGACGACGTCACCATCCAGGTGCTCACCCAATGCCGTCCCGAGCTGATCGAGCGCACCTTCCAGGCGTGTTCGGGC GCACACCGGGCCATCGTGCACTTCTACAACTCGACGTCAATCCTGCAGCGCCGCGTGGTCTTTCGCGCCAACCGGGCTGA GGTGCAGGCCATCGCGACAGATGGGGCGCGCAAGTGCGTCGAGCAGGCCGCCAAATACCCGGGCACGCAGTGGCGATTCG AGTACTCCCCGGAGTCCTACACCGGCACCGAACTGGAATACGCCAAACAGGTGTGCGACGCCGTCGGCGAGGTCATTGCG CCGACGCCGGAGCGCCCGATCATCTTCAACCTGCCCGCCACGGTGGAGATGACGACGCCCAATGTCTACGCCGACTCGAT CGAGTGGATGAGCCGCAACCTAGCCAACCGGGAGTCGGTCATCCTGAGCCTGCACCCGCACAATGACCGCGGAACCGCCG TCGCCGCAGCGGAATTGGGTTTCGCGGCCGGGGCTGATCGGATCGAGGGCTGCCTGTTCGGCAACGGCGAGCGCACCGGC AACGTGTGCCTGGTCACGCTGGGACTCAACCTGTTCTCCCGAGGTGTGGACCCGCAGATCGACTTCTCCAATATTGACGA GATCCGGCGCACGGTGGAGTACTGCAACCAGCTGCCGGTGCACGAACGTCACCCCTATGGCGGCGACCTGGTGTACACCG CGTTCTCCGGTAGCCACCAGGACGCCATCAACAAGGGCCTAGACGCGATGAAGCTGGATGCGGATGCCGCCGACTGTGAC GTCGACGACATGCTGTGGCAGGTGCCGTATCTGCCCATCGACCCGCGCGATGTCGGGCGCACCTACGAGGCGGTGATCCG GGTCAACTCGCAGTCCGGCAAGGGCGGCGTGGCCTACATCATGAAGACCGACCACGGCCTTTCCCTGCCGCGGCGGCTGC AGATCGAGTTTTCCCAGGTAATCCAGAAGATCGCAGAGGGTACAGCAGGCGAGGGTGGCGAGGTCTCGCCCAAGGAGATG TGGGATGCGTTCGCCGAGGAGTATCTGGCCCCGGTGCGGCCTTTGGAGCGGATAAGGCAACATGTGGACGCTGCCGACGA CGACGGCGGCACGACCAGCATCACGGCGACCGTCAAGATCAACGGCGTGGAGACCGAGATCAGCGGGTCCGGTAACGGTC CGTTGGCCGCGTTCGTCCATGCGCTGGCCGATGTCGGGTTTGACGTGGCCGTGCTGGACTACTACGAGCACGCGATGAGC GCCGGCGACGACGCTCAGGCCGCCGCGTATGTGGAGGCCTCCGTGACGATCGCGAGCCCGGCGCAGCCGGGCGAAGCGGG TCGGCACGCATCGGACCCCGTGACGATCGCGAGCCCGGCGCAGCCGGGCGAAGCGGGTCGGCACGCATCGGACCCCGTGA CGATCGCGAGCCCGGCGCAGCCGGGCGAAGCGGGTCGGCACGCATCGGACCCCGTGACGATCGCGAGCCCGGCGCAGCCG GGCGAAGCGGGTCGGCACGCATCGGACCCCGTGACGATCGCGAGCCCGGCGCAGCCGGGCGAAGCGGGTCGGCACGCATC GGACCCCGTGACGATCGCGAGCCCGGCGCAGCCGGGCGAAGCGGGTCGGCACGCATCGGACCCCGTGACGATCGCGAGCC CGGCGCAGCCGGGCGAAGCGGGTCGGCACGCATCGGACCCCGTGACGATCGCGAGCCCGGCGCAGCCGGGCGAAGCGGGT CGGCACGCATCGGACCCCGTGACGATCGCGAGCCCGGCGCAGCCGGGCGAAGCGGGTCGGCACGCATCGGACCCCGTGAC GATCGCGAGCCCGGCGCAGCCGGGCGAAGCGGGTCGGCACGCATCGGACCCCGTGACGATCGCGAGCCCGGCGCAGCCGG GCGAAGCGGGTCGGCACGCATCGGACCCCGTGACGATCGCGAGCCCGGCGCAGCCGGGCGAAGCGGGTCGGCACGCATCG GACCCCGTGACGAGTAAGACGGTGTGGGGTGTCGGTATCGCACCGTCAATCACCACCGCGTCGCTGCGCGCCGTGGTGTC GGCGGTCAACCGGGCGGCACGCTAG
Upstream 100 bases:
>100_bases CGGACGCCGCGACGGGGTCTGATCCAGACCGGCTTCCCGTCGCGGGACGTTCGCGATGCGCCGGTCTGAGGTTCCTTCTC ACCATCCCGGAGCAACTACC
Downstream 100 bases:
>100_bases GACGGCGCTGAACTAGGGTCGGGGTCCGCGGCATGATTTTTCGCAGTGACGTTCCGCTCGCCGTTTCAGAACAACGCTAA CTGCTTTTCGACGGGAGCGA
Product: 2-isopropylmalate synthase
Products: NA
Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]
Number of amino acids: Translated: 834; Mature: 833
Protein sequence:
>834_residues MTTSESPDAYTESFGAHTIVKPAGPPRVGQPSWNPQRASSMPVNRYRPFAEEVEPIRLRNRTWPDRVIDRAPLWCAVDLR DGNQALIDPMSPARKRRMFDLLVRMGYKEIEVGFPSASQTDFDFVREIIEQGAIPDDVTIQVLTQCRPELIERTFQACSG AHRAIVHFYNSTSILQRRVVFRANRAEVQAIATDGARKCVEQAAKYPGTQWRFEYSPESYTGTELEYAKQVCDAVGEVIA PTPERPIIFNLPATVEMTTPNVYADSIEWMSRNLANRESVILSLHPHNDRGTAVAAAELGFAAGADRIEGCLFGNGERTG NVCLVTLGLNLFSRGVDPQIDFSNIDEIRRTVEYCNQLPVHERHPYGGDLVYTAFSGSHQDAINKGLDAMKLDADAADCD VDDMLWQVPYLPIDPRDVGRTYEAVIRVNSQSGKGGVAYIMKTDHGLSLPRRLQIEFSQVIQKIAEGTAGEGGEVSPKEM WDAFAEEYLAPVRPLERIRQHVDAADDDGGTTSITATVKINGVETEISGSGNGPLAAFVHALADVGFDVAVLDYYEHAMS AGDDAQAAAYVEASVTIASPAQPGEAGRHASDPVTIASPAQPGEAGRHASDPVTIASPAQPGEAGRHASDPVTIASPAQP GEAGRHASDPVTIASPAQPGEAGRHASDPVTIASPAQPGEAGRHASDPVTIASPAQPGEAGRHASDPVTIASPAQPGEAG RHASDPVTIASPAQPGEAGRHASDPVTIASPAQPGEAGRHASDPVTIASPAQPGEAGRHASDPVTIASPAQPGEAGRHAS DPVTSKTVWGVGIAPSITTASLRAVVSAVNRAAR
Sequences:
>Translated_834_residues MTTSESPDAYTESFGAHTIVKPAGPPRVGQPSWNPQRASSMPVNRYRPFAEEVEPIRLRNRTWPDRVIDRAPLWCAVDLR DGNQALIDPMSPARKRRMFDLLVRMGYKEIEVGFPSASQTDFDFVREIIEQGAIPDDVTIQVLTQCRPELIERTFQACSG AHRAIVHFYNSTSILQRRVVFRANRAEVQAIATDGARKCVEQAAKYPGTQWRFEYSPESYTGTELEYAKQVCDAVGEVIA PTPERPIIFNLPATVEMTTPNVYADSIEWMSRNLANRESVILSLHPHNDRGTAVAAAELGFAAGADRIEGCLFGNGERTG NVCLVTLGLNLFSRGVDPQIDFSNIDEIRRTVEYCNQLPVHERHPYGGDLVYTAFSGSHQDAINKGLDAMKLDADAADCD VDDMLWQVPYLPIDPRDVGRTYEAVIRVNSQSGKGGVAYIMKTDHGLSLPRRLQIEFSQVIQKIAEGTAGEGGEVSPKEM WDAFAEEYLAPVRPLERIRQHVDAADDDGGTTSITATVKINGVETEISGSGNGPLAAFVHALADVGFDVAVLDYYEHAMS AGDDAQAAAYVEASVTIASPAQPGEAGRHASDPVTIASPAQPGEAGRHASDPVTIASPAQPGEAGRHASDPVTIASPAQP GEAGRHASDPVTIASPAQPGEAGRHASDPVTIASPAQPGEAGRHASDPVTIASPAQPGEAGRHASDPVTIASPAQPGEAG RHASDPVTIASPAQPGEAGRHASDPVTIASPAQPGEAGRHASDPVTIASPAQPGEAGRHASDPVTIASPAQPGEAGRHAS DPVTSKTVWGVGIAPSITTASLRAVVSAVNRAAR >Mature_833_residues TTSESPDAYTESFGAHTIVKPAGPPRVGQPSWNPQRASSMPVNRYRPFAEEVEPIRLRNRTWPDRVIDRAPLWCAVDLRD GNQALIDPMSPARKRRMFDLLVRMGYKEIEVGFPSASQTDFDFVREIIEQGAIPDDVTIQVLTQCRPELIERTFQACSGA HRAIVHFYNSTSILQRRVVFRANRAEVQAIATDGARKCVEQAAKYPGTQWRFEYSPESYTGTELEYAKQVCDAVGEVIAP TPERPIIFNLPATVEMTTPNVYADSIEWMSRNLANRESVILSLHPHNDRGTAVAAAELGFAAGADRIEGCLFGNGERTGN VCLVTLGLNLFSRGVDPQIDFSNIDEIRRTVEYCNQLPVHERHPYGGDLVYTAFSGSHQDAINKGLDAMKLDADAADCDV DDMLWQVPYLPIDPRDVGRTYEAVIRVNSQSGKGGVAYIMKTDHGLSLPRRLQIEFSQVIQKIAEGTAGEGGEVSPKEMW DAFAEEYLAPVRPLERIRQHVDAADDDGGTTSITATVKINGVETEISGSGNGPLAAFVHALADVGFDVAVLDYYEHAMSA GDDAQAAAYVEASVTIASPAQPGEAGRHASDPVTIASPAQPGEAGRHASDPVTIASPAQPGEAGRHASDPVTIASPAQPG EAGRHASDPVTIASPAQPGEAGRHASDPVTIASPAQPGEAGRHASDPVTIASPAQPGEAGRHASDPVTIASPAQPGEAGR HASDPVTIASPAQPGEAGRHASDPVTIASPAQPGEAGRHASDPVTIASPAQPGEAGRHASDPVTIASPAQPGEAGRHASD PVTSKTVWGVGIAPSITTASLRAVVSAVNRAAR
Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]
COG id: COG0119
COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily [H]
Homologues:
Organism=Homo sapiens, GI238624188, Length=222, Percent_Identity=31.981981981982, Blast_Score=79, Evalue=2e-14, Organism=Escherichia coli, GI1786261, Length=312, Percent_Identity=28.8461538461538, Blast_Score=87, Evalue=5e-18, Organism=Saccharomyces cerevisiae, GI6324225, Length=539, Percent_Identity=47.6808905380334, Blast_Score=500, Evalue=1e-142, Organism=Saccharomyces cerevisiae, GI6324682, Length=538, Percent_Identity=46.4684014869888, Blast_Score=487, Evalue=1e-138,
Paralogues:
None
Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR013709 - InterPro: IPR002034 - InterPro: IPR013785 - InterPro: IPR005668 - InterPro: IPR000891 [H]
Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]
EC number: =2.3.3.13 [H]
Molecular weight: Translated: 88584; Mature: 88453
Theoretical pI: Translated: 4.92; Mature: 4.92
Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.1 %Cys (Translated Protein) 1.4 %Met (Translated Protein) 2.5 %Cys+Met (Translated Protein) 1.1 %Cys (Mature Protein) 1.3 %Met (Mature Protein) 2.4 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MTTSESPDAYTESFGAHTIVKPAGPPRVGQPSWNPQRASSMPVNRYRPFAEEVEPIRLRN CCCCCCCCHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCCCCHHHCCCCHHHCCHHHHCC RTWPDRVIDRAPLWCAVDLRDGNQALIDPMSPARKRRMFDLLVRMGYKEIEVGFPSASQT CCCHHHHHCCCCCEEEEEECCCCEEEECCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCC DFDFVREIIEQGAIPDDVTIQVLTQCRPELIERTFQACSGAHRAIVHFYNSTSILQRRVV CHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHH FRANRAEVQAIATDGARKCVEQAAKYPGTQWRFEYSPESYTGTELEYAKQVCDAVGEVIA HHCCHHHEEEEHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHC PTPERPIIFNLPATVEMTTPNVYADSIEWMSRNLANRESVILSLHPHNDRGTAVAAAELG CCCCCCEEEECCCEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEHHHHH FAAGADRIEGCLFGNGERTGNVCLVTLGLNLFSRGVDPQIDFSNIDEIRRTVEYCNQLPV HHCCHHHHCEEEECCCCCCCCEEEEEECHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCC HERHPYGGDLVYTAFSGSHQDAINKGLDAMKLDADAADCDVDDMLWQVPYLPIDPRDVGR CCCCCCCCCEEEEEECCCCHHHHHCCCCCEEECCCCCCCCHHHHHHHCCCCCCCHHHHCC TYEAVIRVNSQSGKGGVAYIMKTDHGLSLPRRLQIEFSQVIQKIAEGTAGEGGEVSPKEM CEEEEEEECCCCCCCCEEEEEECCCCCCCCCEEEHHHHHHHHHHHCCCCCCCCCCCHHHH WDAFAEEYLAPVRPLERIRQHVDAADDDGGTTSITATVKINGVETEISGSGNGPLAAFVH HHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECEEEEECCCCCCHHHHHHH ALADVGFDVAVLDYYEHAMSAGDDAQAAAYVEASVTIASPAQPGEAGRHASDPVTIASPA HHHHCCCCHHHHHHHHHHHCCCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCEEEECCC QPGEAGRHASDPVTIASPAQPGEAGRHASDPVTIASPAQPGEAGRHASDPVTIASPAQPG CCCCCCCCCCCCEEEECCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCEEEECCCCCC EAGRHASDPVTIASPAQPGEAGRHASDPVTIASPAQPGEAGRHASDPVTIASPAQPGEAG CCCCCCCCCEEEECCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCEEEECCCCCCCCC RHASDPVTIASPAQPGEAGRHASDPVTIASPAQPGEAGRHASDPVTIASPAQPGEAGRHA CCCCCCEEEECCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCEEEECCCCCCCCCCCC SDPVTIASPAQPGEAGRHASDPVTSKTVWGVGIAPSITTASLRAVVSAVNRAAR CCCEEEECCCCCCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHCC >Mature Secondary Structure TTSESPDAYTESFGAHTIVKPAGPPRVGQPSWNPQRASSMPVNRYRPFAEEVEPIRLRN CCCCCCCHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCCCCHHHCCCCHHHCCHHHHCC RTWPDRVIDRAPLWCAVDLRDGNQALIDPMSPARKRRMFDLLVRMGYKEIEVGFPSASQT CCCHHHHHCCCCCEEEEEECCCCEEEECCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCC DFDFVREIIEQGAIPDDVTIQVLTQCRPELIERTFQACSGAHRAIVHFYNSTSILQRRVV CHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHH FRANRAEVQAIATDGARKCVEQAAKYPGTQWRFEYSPESYTGTELEYAKQVCDAVGEVIA HHCCHHHEEEEHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHC PTPERPIIFNLPATVEMTTPNVYADSIEWMSRNLANRESVILSLHPHNDRGTAVAAAELG CCCCCCEEEECCCEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEHHHHH FAAGADRIEGCLFGNGERTGNVCLVTLGLNLFSRGVDPQIDFSNIDEIRRTVEYCNQLPV HHCCHHHHCEEEECCCCCCCCEEEEEECHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCC HERHPYGGDLVYTAFSGSHQDAINKGLDAMKLDADAADCDVDDMLWQVPYLPIDPRDVGR CCCCCCCCCEEEEEECCCCHHHHHCCCCCEEECCCCCCCCHHHHHHHCCCCCCCHHHHCC TYEAVIRVNSQSGKGGVAYIMKTDHGLSLPRRLQIEFSQVIQKIAEGTAGEGGEVSPKEM CEEEEEEECCCCCCCCEEEEEECCCCCCCCCEEEHHHHHHHHHHHCCCCCCCCCCCHHHH WDAFAEEYLAPVRPLERIRQHVDAADDDGGTTSITATVKINGVETEISGSGNGPLAAFVH HHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECEEEEECCCCCCHHHHHHH ALADVGFDVAVLDYYEHAMSAGDDAQAAAYVEASVTIASPAQPGEAGRHASDPVTIASPA HHHHCCCCHHHHHHHHHHHCCCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCEEEECCC QPGEAGRHASDPVTIASPAQPGEAGRHASDPVTIASPAQPGEAGRHASDPVTIASPAQPG CCCCCCCCCCCCEEEECCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCEEEECCCCCC EAGRHASDPVTIASPAQPGEAGRHASDPVTIASPAQPGEAGRHASDPVTIASPAQPGEAG CCCCCCCCCEEEECCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCEEEECCCCCCCCC RHASDPVTIASPAQPGEAGRHASDPVTIASPAQPGEAGRHASDPVTIASPAQPGEAGRHA CCCCCCEEEECCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCEEEECCCCCCCCCCCC SDPVTIASPAQPGEAGRHASDPVTSKTVWGVGIAPSITTASLRAVVSAVNRAAR CCCEEEECCCCCCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 12788972 [H]