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The map label for this gene is istB [H]

Identifier: 121639551

GI number: 121639551

Start: 4050005

End: 4050751

Strand: Direct

Name: istB [H]

Synonym: BCG_3696

Alternate gene names: 121639551

Gene position: 4050005-4050751 (Clockwise)

Preceding gene: 121639550

Following gene: 121639556

Centisome position: 92.58

GC content: 65.19

Gene sequence:

>747_bases
ATGGCAGCCAAGACCGCTACCAACAGCCGCGATGTGGCCGCCGAGCTGGCGTATCTGACCCGGGCGCTGAAAGCCCCCAC
CCTGCGCGGGGCCATCGAGCAGCTCGCTGACCGCGCCCGCACCAAGACTTGGAGCTATGAGGAGTTCCTCGCAGCGTGTC
TGCAACGCGAGGTGTCGGCCCGCGAATCCCACGGCGGCGAAGGACGCATCAGGGCCGCCCGCTTCCCATCGCGCAAGTCG
TTGGAGGAGTTCGACTTCGACCACGCCCGCGGTCTCAAACGCGACACCATAGCGCATCTGGGCACCCTGGACTTCGTCAC
CCTAGCAATCGGGATCGCGATCCGCGCCTGCCAGGCCGGCCACCGCGTCCTATTCGCCACCGCCTCGCAATGGGTTGATC
GTCTGGCCGCCGCCCACCACAGCGGCACCCTGCAATCTGAACTGATTCGGCTGGCCCGATACCCGCTGCTGGTCGTCGAC
GAAGTGGGCTACATCCCCTTCGAACCCGAAGCCGCCAACCTGTTCTTCCAATTGGTGTCGTCCCGCTACGAACGGGCCAG
CCTCATCGTCACGTCAAATAAGCCCTTCGGGCGCTGGGGCGAAGTATTCGGCGACGACGTCGTAGCCGCGGCCATGATCG
ACCGACTCGTGCACCACGCCGAAGTCATCGCACTCAAAGGAGACAGCTACCGCATCAAAGACCGAGACCTCGGCCGCGTC
CCCACCGTCACGGCCGACGACCAATGA

Upstream 100 bases:

>100_bases
AACGGTTCGACATCGTCGGTCCACCCCACCACGTTGAGGTCGAACAACGTCTCCTGACCACCTACGACACCGTGTTGGGC
CTTGACGGGCCGGTGGCCTG

Downstream 100 bases:

>100_bases
AACCAAGCTGGTCAATTTTCGATTGCCGACACCTGATCAGTTTTCGGTTGCCGTTGACATAGTGCCCAAAACACGCACCC
ACATCAGATGCAGAACCCCT

Product: transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 248; Mature: 247

Protein sequence:

>248_residues
MAAKTATNSRDVAAELAYLTRALKAPTLRGAIEQLADRARTKTWSYEEFLAACLQREVSARESHGGEGRIRAARFPSRKS
LEEFDFDHARGLKRDTIAHLGTLDFVTLAIGIAIRACQAGHRVLFATASQWVDRLAAAHHSGTLQSELIRLARYPLLVVD
EVGYIPFEPEAANLFFQLVSSRYERASLIVTSNKPFGRWGEVFGDDVVAAAMIDRLVHHAEVIALKGDSYRIKDRDLGRV
PTVTADDQ

Sequences:

>Translated_248_residues
MAAKTATNSRDVAAELAYLTRALKAPTLRGAIEQLADRARTKTWSYEEFLAACLQREVSARESHGGEGRIRAARFPSRKS
LEEFDFDHARGLKRDTIAHLGTLDFVTLAIGIAIRACQAGHRVLFATASQWVDRLAAAHHSGTLQSELIRLARYPLLVVD
EVGYIPFEPEAANLFFQLVSSRYERASLIVTSNKPFGRWGEVFGDDVVAAAMIDRLVHHAEVIALKGDSYRIKDRDLGRV
PTVTADDQ
>Mature_247_residues
AAKTATNSRDVAAELAYLTRALKAPTLRGAIEQLADRARTKTWSYEEFLAACLQREVSARESHGGEGRIRAARFPSRKSL
EEFDFDHARGLKRDTIAHLGTLDFVTLAIGIAIRACQAGHRVLFATASQWVDRLAAAHHSGTLQSELIRLARYPLLVVDE
VGYIPFEPEAANLFFQLVSSRYERASLIVTSNKPFGRWGEVFGDDVVAAAMIDRLVHHAEVIALKGDSYRIKDRDLGRVP
TVTADDQ

Specific function: One of two proteins expressed only when there is a tandem repeat of the IS21 insertion sequence, it is necessary for the transposition of plasmids with that tandem repeat [H]

COG id: COG1484

COG function: function code L; DNA replication protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IS21/IS1162 putative ATP-binding protein family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001270
- InterPro:   IPR002611 [H]

Pfam domain/function: PF01695 IstB [H]

EC number: NA

Molecular weight: Translated: 27490; Mature: 27358

Theoretical pI: Translated: 8.25; Mature: 8.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAKTATNSRDVAAELAYLTRALKAPTLRGAIEQLADRARTKTWSYEEFLAACLQREVSA
CCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
RESHGGEGRIRAARFPSRKSLEEFDFDHARGLKRDTIAHLGTLDFVTLAIGIAIRACQAG
HHCCCCCCCEEEECCCCCCCHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
HRVLFATASQWVDRLAAAHHSGTLQSELIRLARYPLLVVDEVGYIPFEPEAANLFFQLVS
CEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHHHHH
SRYERASLIVTSNKPFGRWGEVFGDDVVAAAMIDRLVHHAEVIALKGDSYRIKDRDLGRV
HHHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEECCCCCCCC
PTVTADDQ
CCCCCCCC
>Mature Secondary Structure 
AAKTATNSRDVAAELAYLTRALKAPTLRGAIEQLADRARTKTWSYEEFLAACLQREVSA
CCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
RESHGGEGRIRAARFPSRKSLEEFDFDHARGLKRDTIAHLGTLDFVTLAIGIAIRACQAG
HHCCCCCCCEEEECCCCCCCHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
HRVLFATASQWVDRLAAAHHSGTLQSELIRLARYPLLVVDEVGYIPFEPEAANLFFQLVS
CEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHHHHH
SRYERASLIVTSNKPFGRWGEVFGDDVVAAAMIDRLVHHAEVIALKGDSYRIKDRDLGRV
HHHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEECCCCCCCC
PTVTADDQ
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2540414 [H]