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The map label for this gene is arsB2 [H]

Identifier: 121639498

GI number: 121639498

Start: 3999034

End: 4000275

Strand: Direct

Name: arsB2 [H]

Synonym: BCG_3643

Alternate gene names: 121639498

Gene position: 3999034-4000275 (Clockwise)

Preceding gene: 121639497

Following gene: 121639504

Centisome position: 91.42

GC content: 69.65

Gene sequence:

>1242_bases
TTGACACTTGCTGTTGCGCTGATACTGCTTGCCGTGGTCCTGGGGTTCGCGGTTGCCCGCCCACGCGGCTGGCCGGAGGC
AGCGGCGGCGGTTCCGGCAGCGGTCATCCTGTTAGCGATCGGGGCGATCTCGCCCCAGCAGGCGATGGCGCAGGTGTCCG
GGCTGGCGCGCGTGGTCGCGTTTCTGGGTGCGGTTCTGGTGCTGGCTAAGCTGTGCGACGACGAAGGCCTGTTCGAGGCA
GCCGGCGCGGCCATGGCTCGAGCGAGCGCGGAGTCGCACCGACTGCTACGGCAGGTGTTCGCCGTCTCGGCCGCCATCAC
CGCGGCGCTCTGCCTGGACGCCACCGTGGTGCTGTTGACCCCGGTGGTGCTGGCGACGGTCCGCCGGCTGCGGACCCCGG
TGCGCCCCTATGCCTACGCCACCGCCCACCTAGCCAACGCCGCTTCGCTGCTGCTTCCGGTGTCGAATCTGACCAACCTG
CTCGCCTACCACGGTGCCGGCATCTCGTTCACCAAGTTCACGCTGCTGATGGCATTGCCTTGGCTGTCCGCCGTGGCCGC
GGTCTATGTGGTCTTCCGCTGGTTTTTCGCCCGGGATCTACGCGTGGTGCCGGACCGGCAGCAACTCAAGCCGGCGCCGC
GCCTGCCAATGTTCGTGCTGGTGGTGGTGGCGCTGACACTCGGGGGCTTCGCCGTCGCCGAGTCGGTGGGACTGGCCCCA
ACGTGGGCGGCGCTGGCTGGCGCCGCAGTGTTGGCGCTGCGAAGTCTGCGGCGTGGACACACTTCGGTGCTGCGGATCGC
GCGCGCCGTCAACGTGTCGTTCCTGGTCTTTGTGTTGGCCCTGGGTGTCGTGGTGCACGCGGTCATGCTCAACGGCATGG
CCGCCAGGATGTCCGCCGTGCTGCCGACCGGGTCCGGGTTGCCCGCGCTGCTCGGCATCGCCGCGCTGGCCTCCGTGCTG
GCCAACGTGGTCAACAACCTGCCCGCGACTCTGGTGTTAGTGCCGCTGGTGGCGGCCGGCGGGCCGGCGGCCGTGCTGGC
CGTGCTACTCGGGGTCAACATCGGACCCAACCTGACCTATGCCGGTTCGCTGTCTAACCTGCTGTGGCGGGGCGTGCTGC
GCCGGCACAACGTCGACGCCAGCGTCGGCGAGTACACCCGACTGGGACTGTGCACCGTGCCTGCGGCCCTGGCGATGGCG
GTGCTCGCGCTGTGGGCCAGCGCCCAGGTTCTGGGGATCTAG

Upstream 100 bases:

>100_bases
ATCCGCATCCTCGACGAGCTGGCCGCCCAGGACGGCGTCGCGCTGCAGATGCCGACGGTGTGGCGCCGCGAGGATCCCTG
GATGTGAAGCGCTCTAGCCC

Downstream 100 bases:

>100_bases
CCGCAAGGGCGCGAGCAGACGCAGAATCGCATGATTTGAGCTCAAATCATGCGATTCTGCGTCTGCTCGCGAGGCTCGCG
TGGCCGCCGGCGCTGGCGGG

Product: putative arsenical pump integral membrane protein arsB2

Products: ADP; phosphate; arsenite [Periplasm] [C]

Alternate protein names: Arsenic efflux pump protein [H]

Number of amino acids: Translated: 413; Mature: 412

Protein sequence:

>413_residues
MTLAVALILLAVVLGFAVARPRGWPEAAAAVPAAVILLAIGAISPQQAMAQVSGLARVVAFLGAVLVLAKLCDDEGLFEA
AGAAMARASAESHRLLRQVFAVSAAITAALCLDATVVLLTPVVLATVRRLRTPVRPYAYATAHLANAASLLLPVSNLTNL
LAYHGAGISFTKFTLLMALPWLSAVAAVYVVFRWFFARDLRVVPDRQQLKPAPRLPMFVLVVVALTLGGFAVAESVGLAP
TWAALAGAAVLALRSLRRGHTSVLRIARAVNVSFLVFVLALGVVVHAVMLNGMAARMSAVLPTGSGLPALLGIAALASVL
ANVVNNLPATLVLVPLVAAGGPAAVLAVLLGVNIGPNLTYAGSLSNLLWRGVLRRHNVDASVGEYTRLGLCTVPAALAMA
VLALWASAQVLGI

Sequences:

>Translated_413_residues
MTLAVALILLAVVLGFAVARPRGWPEAAAAVPAAVILLAIGAISPQQAMAQVSGLARVVAFLGAVLVLAKLCDDEGLFEA
AGAAMARASAESHRLLRQVFAVSAAITAALCLDATVVLLTPVVLATVRRLRTPVRPYAYATAHLANAASLLLPVSNLTNL
LAYHGAGISFTKFTLLMALPWLSAVAAVYVVFRWFFARDLRVVPDRQQLKPAPRLPMFVLVVVALTLGGFAVAESVGLAP
TWAALAGAAVLALRSLRRGHTSVLRIARAVNVSFLVFVLALGVVVHAVMLNGMAARMSAVLPTGSGLPALLGIAALASVL
ANVVNNLPATLVLVPLVAAGGPAAVLAVLLGVNIGPNLTYAGSLSNLLWRGVLRRHNVDASVGEYTRLGLCTVPAALAMA
VLALWASAQVLGI
>Mature_412_residues
TLAVALILLAVVLGFAVARPRGWPEAAAAVPAAVILLAIGAISPQQAMAQVSGLARVVAFLGAVLVLAKLCDDEGLFEAA
GAAMARASAESHRLLRQVFAVSAAITAALCLDATVVLLTPVVLATVRRLRTPVRPYAYATAHLANAASLLLPVSNLTNLL
AYHGAGISFTKFTLLMALPWLSAVAAVYVVFRWFFARDLRVVPDRQQLKPAPRLPMFVLVVVALTLGGFAVAESVGLAPT
WAALAGAAVLALRSLRRGHTSVLRIARAVNVSFLVFVLALGVVVHAVMLNGMAARMSAVLPTGSGLPALLGIAALASVLA
NVVNNLPATLVLVPLVAAGGPAAVLAVLLGVNIGPNLTYAGSLSNLLWRGVLRRHNVDASVGEYTRLGLCTVPAALAMAV
LALWASAQVLGI

Specific function: Involved in arsenical resistance. Thought to form the channel of an arsenite pump [H]

COG id: COG1055

COG function: function code P; Na+/H+ antiporter NhaD and related arsenite permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ArsB family [H]

Homologues:

Organism=Escherichia coli, GI87082277, Length=415, Percent_Identity=30.1204819277108, Blast_Score=105, Evalue=6e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000802 [H]

Pfam domain/function: PF02040 ArsB [H]

EC number: NA

Molecular weight: Translated: 42560; Mature: 42428

Theoretical pI: Translated: 11.71; Mature: 11.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TLAVALILLAVVLGFAVARPRGWPEAAAAVPAAVILLAIGAISPQQAMAQVSGLARVVA
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
FLGAVLVLAKLCDDEGLFEAAGAAMARASAESHRLLRQVFAVSAAITAALCLDATVVLLT
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PVVLATVRRLRTPVRPYAYATAHLANAASLLLPVSNLTNLLAYHGAGISFTKFTLLMALP
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
WLSAVAAVYVVFRWFFARDLRVVPDRQQLKPAPRLPMFVLVVVALTLGGFAVAESVGLAP
HHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCH
TWAALAGAAVLALRSLRRGHTSVLRIARAVNVSFLVFVLALGVVVHAVMLNGMAARMSAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LPTGSGLPALLGIAALASVLANVVNNLPATLVLVPLVAAGGPAAVLAVLLGVNIGPNLTY
CCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCC
AGSLSNLLWRGVLRRHNVDASVGEYTRLGLCTVPAALAMAVLALWASAQVLGI
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; arsenite [Cytoplasm]; H2O [C]

Specific reaction: ATP + arsenite [Cytoplasm] + H2O = ADP + phosphate + arsenite [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12950922 [H]