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The map label for this gene is otsA

Identifier: 121639409

GI number: 121639409

Start: 3887733

End: 3889235

Strand: Direct

Name: otsA

Synonym: BCG_3554

Alternate gene names: 121639409

Gene position: 3887733-3889235 (Clockwise)

Preceding gene: 121639408

Following gene: 121639410

Centisome position: 88.87

GC content: 65.6

Gene sequence:

>1503_bases
ATGGCTCCCTCGGGAGGCCAGGAGGCGCAGATTTGCGATTCGGAGACCTTCGGGGACTCTGACTTCGTGGTGGTAGCCAA
TCGACTGCCCGTCGATCTGGAGCGTCTTCCCGACGGCAGCACAACCTGGAAACGCAGCCCCGGAGGCTTGGTCACCGCCT
TGGAGCCGGTGCTGCGGCGTCGGCGCGGGGCCTGGGTCGGCTGGCCCGGCGTTAACGACGACGGGGCCGAACCCGACCTC
CACGTGCTGGACGGCCCCATCATCCAAGACGAGCTGGAACTTCATCCGGTACGGCTGAGCACCACGGACATAGCTCAGTA
CTACGAGGGATTCTCCAACGCCACACTGTGGCCGCTGTACCACGACGTCATCGTCAAGCCGCTCTACCACCGCGAATGGT
GGGATCGCTACGTCGACGTCAACCAGCGCTTTGCCGAGGCCGCGTCGCGCGCCGCCGCCCACGGCGCAACCGTGTGGGTA
CAGGACTACCAGCTGCAGCTGGTACCGAAGATGCTGCGCATGCTGCGGCCCGATCTGACCATCGGTTTCTTTTTGCACAT
CCCGTTCCCGCCGGTAGAGCTGTTTATGCAGATGCCGTGGCGCACCGAGATCATCCAGGGCCTACTGGGCGCCGACCTGG
TGGGCTTCCATCTTCCGGGCGGTGCCCAGAATTTCCTGATCCTGTCCCGGCGTCTGGTCGGCACCGACACTTCCCGCGGA
ACCGTCGGTGTGCGGTCGCGGTTCGGTGCGGCGGTGCTCGGGTCCCGCACCATACGAGTTGGCGCCTTTCCTATCTCGGT
TGACTCCGGCGCGCTCGACCACGCTGCCCGCGACCGCAACATCAGGCGCCGGGCCCGCGAGATTCGCACCGAACTGGGAA
ATCCGCGCAAGATCCTGCTCGGTGTTGACCGGCTCGACTACACCAAGGGCATCGACGTACGGCTGAAGGCCTTTTCCGAG
CTGCTGGCCGAGGGCCGCGTCAAACGCGACGACACCGTCCTGGTCCAGCTGGCTACCCCGAGCCGCGAGCGGGTGGAGAG
CTACCAGACGCTGCGCAACGACATCGAACGCCAGGTCGGCCACATTAACGGCGAGTACGGTGAGGTTGGCCATCCGGTAG
TGCATTACCTGCATCGACCGGCTCCGCGCGACGAGCTTATCGCTTTCTTCGTGGCCAGCGACGTCATGCTGGTCACCCCA
CTACGCGACGGGATGAACCTGGTGGCCAAGGAGTACGTCGCTTGCCGCAGCGATCTTGGCGGTGCCCTGGTGCTCAGCGA
ATTCACCGGGGCCGCAGCCGAACTCCGGCACGCATACCTGGTCAACCCGCACGACCTGGAAGGCGTCAAGGACGGGATAG
AGGAAGCGCTCAACCAGACGGAGGAGGCGGGCCGGCGGCGAATGCGGTCGCTGCGACGCCAAGTGCTCGCCCACGACGTG
GACCGCTGGGCACAGTCGTTTCTCGACGCTCTCGCCGGGGCACACCCGAGGGGCCAAGGCTAA

Upstream 100 bases:

>100_bases
GCCAGGCCAGGCGAGTCGTCGCCGCATATGCGAACGACGCCGACGAGTGTCGGATCTTCCTGTCCATGCTCGGTATTGGA
CCGGCCAAACTCGAGAGCTA

Downstream 100 bases:

>100_bases
CGGTCAAGCCGCTCCCGCTCGCGAGCAGACGCAGAATCGCCCATTTCGGCACGAAATTGGGCGATTCTGCGTCTGCTCGC
GCCCTGGAAGCTGGTGCGGC

Product: putative alpha, alpha-trehalose-phosphate synthase [udp-forming] otsA

Products: UDP; alpha,alpha'-Trehalose 6-phosphate

Alternate protein names: TPS; Trehalose-6-phosphate synthase

Number of amino acids: Translated: 500; Mature: 499

Protein sequence:

>500_residues
MAPSGGQEAQICDSETFGDSDFVVVANRLPVDLERLPDGSTTWKRSPGGLVTALEPVLRRRRGAWVGWPGVNDDGAEPDL
HVLDGPIIQDELELHPVRLSTTDIAQYYEGFSNATLWPLYHDVIVKPLYHREWWDRYVDVNQRFAEAASRAAAHGATVWV
QDYQLQLVPKMLRMLRPDLTIGFFLHIPFPPVELFMQMPWRTEIIQGLLGADLVGFHLPGGAQNFLILSRRLVGTDTSRG
TVGVRSRFGAAVLGSRTIRVGAFPISVDSGALDHAARDRNIRRRAREIRTELGNPRKILLGVDRLDYTKGIDVRLKAFSE
LLAEGRVKRDDTVLVQLATPSRERVESYQTLRNDIERQVGHINGEYGEVGHPVVHYLHRPAPRDELIAFFVASDVMLVTP
LRDGMNLVAKEYVACRSDLGGALVLSEFTGAAAELRHAYLVNPHDLEGVKDGIEEALNQTEEAGRRRMRSLRRQVLAHDV
DRWAQSFLDALAGAHPRGQG

Sequences:

>Translated_500_residues
MAPSGGQEAQICDSETFGDSDFVVVANRLPVDLERLPDGSTTWKRSPGGLVTALEPVLRRRRGAWVGWPGVNDDGAEPDL
HVLDGPIIQDELELHPVRLSTTDIAQYYEGFSNATLWPLYHDVIVKPLYHREWWDRYVDVNQRFAEAASRAAAHGATVWV
QDYQLQLVPKMLRMLRPDLTIGFFLHIPFPPVELFMQMPWRTEIIQGLLGADLVGFHLPGGAQNFLILSRRLVGTDTSRG
TVGVRSRFGAAVLGSRTIRVGAFPISVDSGALDHAARDRNIRRRAREIRTELGNPRKILLGVDRLDYTKGIDVRLKAFSE
LLAEGRVKRDDTVLVQLATPSRERVESYQTLRNDIERQVGHINGEYGEVGHPVVHYLHRPAPRDELIAFFVASDVMLVTP
LRDGMNLVAKEYVACRSDLGGALVLSEFTGAAAELRHAYLVNPHDLEGVKDGIEEALNQTEEAGRRRMRSLRRQVLAHDV
DRWAQSFLDALAGAHPRGQG
>Mature_499_residues
APSGGQEAQICDSETFGDSDFVVVANRLPVDLERLPDGSTTWKRSPGGLVTALEPVLRRRRGAWVGWPGVNDDGAEPDLH
VLDGPIIQDELELHPVRLSTTDIAQYYEGFSNATLWPLYHDVIVKPLYHREWWDRYVDVNQRFAEAASRAAAHGATVWVQ
DYQLQLVPKMLRMLRPDLTIGFFLHIPFPPVELFMQMPWRTEIIQGLLGADLVGFHLPGGAQNFLILSRRLVGTDTSRGT
VGVRSRFGAAVLGSRTIRVGAFPISVDSGALDHAARDRNIRRRAREIRTELGNPRKILLGVDRLDYTKGIDVRLKAFSEL
LAEGRVKRDDTVLVQLATPSRERVESYQTLRNDIERQVGHINGEYGEVGHPVVHYLHRPAPRDELIAFFVASDVMLVTPL
RDGMNLVAKEYVACRSDLGGALVLSEFTGAAAELRHAYLVNPHDLEGVKDGIEEALNQTEEAGRRRMRSLRRQVLAHDVD
RWAQSFLDALAGAHPRGQG

Specific function: Catalyzes the transfer of glucose from a nucleoside diphosphate-glucose to glucose-6-phosphate to form trehalose-6- phosphate and nucleoside diphosphate

COG id: COG0380

COG function: function code G; Trehalose-6-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 20 family

Homologues:

Organism=Escherichia coli, GI1788206, Length=480, Percent_Identity=33.3333333333333, Blast_Score=233, Evalue=2e-62,
Organism=Caenorhabditis elegans, GI115533560, Length=547, Percent_Identity=27.4223034734918, Blast_Score=190, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI115533558, Length=547, Percent_Identity=27.4223034734918, Blast_Score=190, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI71987012, Length=559, Percent_Identity=26.2969588550984, Blast_Score=165, Evalue=5e-41,
Organism=Saccharomyces cerevisiae, GI6319602, Length=477, Percent_Identity=36.6876310272537, Blast_Score=314, Evalue=2e-86,
Organism=Saccharomyces cerevisiae, GI6323537, Length=446, Percent_Identity=28.9237668161435, Blast_Score=195, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6320279, Length=488, Percent_Identity=30.327868852459, Blast_Score=193, Evalue=6e-50,
Organism=Saccharomyces cerevisiae, GI6323917, Length=454, Percent_Identity=28.4140969162996, Blast_Score=191, Evalue=2e-49,
Organism=Drosophila melanogaster, GI19920676, Length=497, Percent_Identity=35.4124748490946, Blast_Score=280, Evalue=2e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): OTSA_MYCBO (Q7TWE1)

Other databases:

- EMBL:   BX248346
- RefSeq:   NP_857159.1
- HSSP:   P31677
- ProteinModelPortal:   Q7TWE1
- SMR:   Q7TWE1
- EnsemblBacteria:   EBMYCT00000014778
- GeneID:   1093120
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb3520
- GeneTree:   EBGT00050000016525
- HOGENOM:   HBG559076
- OMA:   NVNNADW
- ProtClustDB:   CLSK872185
- BioCyc:   MBOV233413:MB3520-MONOMER
- InterPro:   IPR001830

Pfam domain/function: PF00982 Glyco_transf_20

EC number: 2.4.1.15

Molecular weight: Translated: 55877; Mature: 55746

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: NA

Important sites: BINDING 28-28 BINDING 108-108 BINDING 162-162 BINDING 304-304 BINDING 309-309 BINDING 342-342

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPSGGQEAQICDSETFGDSDFVVVANRLPVDLERLPDGSTTWKRSPGGLVTALEPVLRR
CCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHH
RRGAWVGWPGVNDDGAEPDLHVLDGPIIQDELELHPVRLSTTDIAQYYEGFSNATLWPLY
HCCCEEECCCCCCCCCCCCCEEECCCCCCCCCCEEEEEECHHHHHHHHHCCCCCEEHHHH
HDVIVKPLYHREWWDRYVDVNQRFAEAASRAAAHGATVWVQDYQLQLVPKMLRMLRPDLT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHCCCCE
IGFFLHIPFPPVELFMQMPWRTEIIQGLLGADLVGFHLPGGAQNFLILSRRLVGTDTSRG
EEEEEECCCCHHHHHHHCCCHHHHHHHHHCCCHHEEECCCCCCCHHHHHHHHHCCCCCCC
TVGVRSRFGAAVLGSRTIRVGAFPISVDSGALDHAARDRNIRRRAREIRTELGNPRKILL
CCCHHHHCCHHHHCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
GVDRLDYTKGIDVRLKAFSELLAEGRVKRDDTVLVQLATPSRERVESYQTLRNDIERQVG
ECCHHCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHC
HINGEYGEVGHPVVHYLHRPAPRDELIAFFVASDVMLVTPLRDGMNLVAKEYVACRSDLG
CCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHCCEEEEEECHHHHHHHHHHHHHHHHCCC
GALVLSEFTGAAAELRHAYLVNPHDLEGVKDGIEEALNQTEEAGRRRMRSLRRQVLAHDV
CEEEEHHHCCHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DRWAQSFLDALAGAHPRGQG
HHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
APSGGQEAQICDSETFGDSDFVVVANRLPVDLERLPDGSTTWKRSPGGLVTALEPVLRR
CCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHH
RRGAWVGWPGVNDDGAEPDLHVLDGPIIQDELELHPVRLSTTDIAQYYEGFSNATLWPLY
HCCCEEECCCCCCCCCCCCCEEECCCCCCCCCCEEEEEECHHHHHHHHHCCCCCEEHHHH
HDVIVKPLYHREWWDRYVDVNQRFAEAASRAAAHGATVWVQDYQLQLVPKMLRMLRPDLT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHCCCCE
IGFFLHIPFPPVELFMQMPWRTEIIQGLLGADLVGFHLPGGAQNFLILSRRLVGTDTSRG
EEEEEECCCCHHHHHHHCCCHHHHHHHHHCCCHHEEECCCCCCCHHHHHHHHHCCCCCCC
TVGVRSRFGAAVLGSRTIRVGAFPISVDSGALDHAARDRNIRRRAREIRTELGNPRKILL
CCCHHHHCCHHHHCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
GVDRLDYTKGIDVRLKAFSELLAEGRVKRDDTVLVQLATPSRERVESYQTLRNDIERQVG
ECCHHCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHC
HINGEYGEVGHPVVHYLHRPAPRDELIAFFVASDVMLVTPLRDGMNLVAKEYVACRSDLG
CCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHCCEEEEEECHHHHHHHHHHHHHHHHCCC
GALVLSEFTGAAAELRHAYLVNPHDLEGVKDGIEEALNQTEEAGRRRMRSLRRQVLAHDV
CEEEEHHHCCHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DRWAQSFLDALAGAHPRGQG
HHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Monovalent cations [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-glucose; D-glucose 6-phosphate

Specific reaction: UDP-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate

General reaction: Hexosyl group transfer [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972