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The map label for this gene is spoU [H]

Identifier: 121639293

GI number: 121639293

Start: 3765292

End: 3765756

Strand: Direct

Name: spoU [H]

Synonym: BCG_3438

Alternate gene names: 121639293

Gene position: 3765292-3765756 (Clockwise)

Preceding gene: 121639287

Following gene: 121639294

Centisome position: 86.07

GC content: 68.39

Gene sequence:

>465_bases
ATGTTCCGGCTGCTGTTCGTATCTCCGCGTATCGCCCCCAACACCGGCAACGCCATCCGGACGTGCGCCGCAACCGGCTG
TGAACTGCATCTGGTCGAGCCGCTCGGCTTCGACCTGTCCGAACCCAAGCTGCGACGGGCCGGGCTGGACTACCACGACC
TGGCCTCGGTCACCGTTCATGCCTCGCTCGCGCACGCCTGGGAGGCGCTGTCGCCAGCGCGGGTGTTCGCCTTCACGGCG
CAGGCGACGACGTTGTTCACCAACGTCGGCTACCGGGCCGGTGACGTGTTGATGTTCGGGCCCGAACCCACCGGCCTGGA
CGAGGCCACCCTGGCTGATACGCACATCACCGGGCAGGTGCGCATTCCGATGCTGGCGGGCCGGCGCTCGTTGAACCTGT
CCAACGCCGCAGCCGTCGCGGTCTACGAGGCCTGGCGTCAGCACGGCTTTGCCGGGGCGGTCTAG

Upstream 100 bases:

>100_bases
CGAGTATGACAGCCCACGGCCGAGGTCTCCACCGCTGTCACCGAGCATGTCACCGGACAGGCCGGCAAACGGGCACCGGG
CGCTTTGCCATGATCGGCGG

Downstream 100 bases:

>100_bases
TCGCGACCAAGGTGACACCGAACCAGCCGGTATGCGCACAACGAAGCTCATCGGCGTCGGGCGCCGGACAGGAGCACCCA
ACCGGTGACAGCACACCGAA

Product: putative tRNA/rRNA methylase spoU

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 154; Mature: 154

Protein sequence:

>154_residues
MFRLLFVSPRIAPNTGNAIRTCAATGCELHLVEPLGFDLSEPKLRRAGLDYHDLASVTVHASLAHAWEALSPARVFAFTA
QATTLFTNVGYRAGDVLMFGPEPTGLDEATLADTHITGQVRIPMLAGRRSLNLSNAAAVAVYEAWRQHGFAGAV

Sequences:

>Translated_154_residues
MFRLLFVSPRIAPNTGNAIRTCAATGCELHLVEPLGFDLSEPKLRRAGLDYHDLASVTVHASLAHAWEALSPARVFAFTA
QATTLFTNVGYRAGDVLMFGPEPTGLDEATLADTHITGQVRIPMLAGRRSLNLSNAAAVAVYEAWRQHGFAGAV
>Mature_154_residues
MFRLLFVSPRIAPNTGNAIRTCAATGCELHLVEPLGFDLSEPKLRRAGLDYHDLASVTVHASLAHAWEALSPARVFAFTA
QATTLFTNVGYRAGDVLMFGPEPTGLDEATLADTHITGQVRIPMLAGRRSLNLSNAAAVAVYEAWRQHGFAGAV

Specific function: Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S- adenosyl-L-methionine to the 2'-OH of the wobble nucleotide [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=154, Percent_Identity=49.3506493506493, Blast_Score=157, Evalue=3e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004440
- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 16517; Mature: 16517

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRLLFVSPRIAPNTGNAIRTCAATGCELHLVEPLGFDLSEPKLRRAGLDYHDLASVTVH
CEEEEEECCEECCCCCCCEEEHCCCCCEEEEECCCCCCCCCCHHHHCCCCHHHHHHEEEH
ASLAHAWEALSPARVFAFTAQATTLFTNVGYRAGDVLMFGPEPTGLDEATLADTHITGQV
HHHHHHHHHCCCCEEEEEECHHEEEEEECCCCCCCEEEECCCCCCCCCCEECCEEECCEE
RIPMLAGRRSLNLSNAAAVAVYEAWRQHGFAGAV
EEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MFRLLFVSPRIAPNTGNAIRTCAATGCELHLVEPLGFDLSEPKLRRAGLDYHDLASVTVH
CEEEEEECCEECCCCCCCEEEHCCCCCEEEEECCCCCCCCCCHHHHCCCCHHHHHHEEEH
ASLAHAWEALSPARVFAFTAQATTLFTNVGYRAGDVLMFGPEPTGLDEATLADTHITGQV
HHHHHHHHHCCCCEEEEEECHHEEEEEECCCCCCCEEEECCCCCCCCCCEECCEEECCEE
RIPMLAGRRSLNLSNAAAVAVYEAWRQHGFAGAV
EEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA