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The map label for this gene is aroA_1 [H]

Identifier: 121639117

GI number: 121639117

Start: 3555859

End: 3557211

Strand: Direct

Name: aroA_1 [H]

Synonym: BCG_3257

Alternate gene names: 121639117

Gene position: 3555859-3557211 (Clockwise)

Preceding gene: 121639114

Following gene: 121639118

Centisome position: 81.29

GC content: 70.44

Gene sequence:

>1353_bases
GTGAAGACATGGCCAGCCCCAACGGCGCCGACGCCGGTGCGCGCTACCGTGACCGTTCCAGGCTCGAAGTCGCAGACCAA
CCGGGCGCTGGTGCTAGCGGCGCTGGCGGCCGCACAAGGCCGGGGCGCATCGACCATCTCCGGCGCGCTGCGCAGCCGCG
ACACCGAACTGATGCTGGACGCGCTGCAGACCCTGGGCCTGCGCGTCGACGGTGTGGGTTCGGAACTGACGGTCAGCGGC
CGAATCGAACCGGGGCCCGGCGCTCGGGTGGACTGTGGCTTGGCGGGCACGGTGTTGCGGTTTGTTCCGCCGCTGGCGGC
GCTGGGCTCCGTCCCGGTCACCTTCGACGGCGATCAGCAAGCCCGGGGACGGCCCATCGCACCGCTGCTGGATGCGCTGC
GCGAGCTCGGCGTCGCCGTCGACGGCACCGGTCTACCGTTTCGGGTTCGCGGCAACGGGTCGCTCGCCGGCGGCACCGTG
GCCATCGACGCGTCGGCGTCCTCACAGTTCGTGTCCGGGCTGCTGCTGTCCGCGGCATCGTTCACCGATGGCCTGACCGT
CCAACACACCGGTTCGTCGCTGCCGTCTGCGCCGCACATCGCGATGACGGCGGCGATGCTGCGGCAAGCCGGAGTCGACA
TCGACGACTCGACACCGAACCGTTGGCAGGTGCGCCCCGGTCCGGTGGCGGCGCGGCGCTGGGACATCGAACCGGACCTG
ACCAACGCGGTGGCTTTCCTGTCAGCGGCCGTGGTCAGCGGCGGCACCGTGCGCATCACCGGCTGGCCTAGAGTCAGCGT
GCAACCCGCCGACCACATCTTGGCAATTTTGCGGCAGCTCAATGCCGTTGTCATTCATGCTGATTCATCCCTCGAGGTGC
GCGGTCCAACGGGATACGACGGGTTTGACGTCGACTTGCGCGCCGTCGGCGAGCTGACGCCATCGGTCGCGGCGCTGGCG
GCGCTGGCATCCCCGGGATCGGTGTCCAGACTAAGCGGCATTGCCCATCTGCGGGGCCACGAAACCGACCGGCTCGCCGC
GCTGAGCACCGAGATCAACCGGTTGGGGGGCACCTGCCGGGAAACACCCGACGGTCTGGTGATCACCGCGACGCCGTTGC
GGCCCGGCATCTGGCGGGCATACGCGGACCATCGAATGGCGATGGCCGGCGCGATCATTGGGCTGCGGGTGGCCGGAGTC
GAGGTCGACGACATCGCCGCCACCACCAAGACGCTGCCGGAGTTTCCGCGGCTGTGGGCCGAGATGGTCGGACCCGGCCA
GGGGTGGGGGTACCCCCAGCCGCGCAGCGGCCAGCGGGCGAGGCGGGCAACCGGGCAGGGGTCCGGCGGTTGA

Upstream 100 bases:

>100_bases
CGGCCAGCTGGGCCGGATCAGTGGTGACCGCAAACCGTCCGCACATGCTTCCTATGGTGCCTGGTACCCACGACACCCGC
CGACACGGCAGGATGAAGCG

Downstream 100 bases:

>100_bases
GGCCCGGCGACTACGACGAGTCCGACGTCAAGGTGCGCTCCGGCAGGAGTTCGCGGCCGCGGACCAAGACCCGTCCCGAG
CACGCCGACGCCGAGGCCGC

Product: 3-phosphoshikimate 1-carboxyvinyltransferase

Products: NA

Alternate protein names: 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS [H]

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MKTWPAPTAPTPVRATVTVPGSKSQTNRALVLAALAAAQGRGASTISGALRSRDTELMLDALQTLGLRVDGVGSELTVSG
RIEPGPGARVDCGLAGTVLRFVPPLAALGSVPVTFDGDQQARGRPIAPLLDALRELGVAVDGTGLPFRVRGNGSLAGGTV
AIDASASSQFVSGLLLSAASFTDGLTVQHTGSSLPSAPHIAMTAAMLRQAGVDIDDSTPNRWQVRPGPVAARRWDIEPDL
TNAVAFLSAAVVSGGTVRITGWPRVSVQPADHILAILRQLNAVVIHADSSLEVRGPTGYDGFDVDLRAVGELTPSVAALA
ALASPGSVSRLSGIAHLRGHETDRLAALSTEINRLGGTCRETPDGLVITATPLRPGIWRAYADHRMAMAGAIIGLRVAGV
EVDDIAATTKTLPEFPRLWAEMVGPGQGWGYPQPRSGQRARRATGQGSGG

Sequences:

>Translated_450_residues
MKTWPAPTAPTPVRATVTVPGSKSQTNRALVLAALAAAQGRGASTISGALRSRDTELMLDALQTLGLRVDGVGSELTVSG
RIEPGPGARVDCGLAGTVLRFVPPLAALGSVPVTFDGDQQARGRPIAPLLDALRELGVAVDGTGLPFRVRGNGSLAGGTV
AIDASASSQFVSGLLLSAASFTDGLTVQHTGSSLPSAPHIAMTAAMLRQAGVDIDDSTPNRWQVRPGPVAARRWDIEPDL
TNAVAFLSAAVVSGGTVRITGWPRVSVQPADHILAILRQLNAVVIHADSSLEVRGPTGYDGFDVDLRAVGELTPSVAALA
ALASPGSVSRLSGIAHLRGHETDRLAALSTEINRLGGTCRETPDGLVITATPLRPGIWRAYADHRMAMAGAIIGLRVAGV
EVDDIAATTKTLPEFPRLWAEMVGPGQGWGYPQPRSGQRARRATGQGSGG
>Mature_450_residues
MKTWPAPTAPTPVRATVTVPGSKSQTNRALVLAALAAAQGRGASTISGALRSRDTELMLDALQTLGLRVDGVGSELTVSG
RIEPGPGARVDCGLAGTVLRFVPPLAALGSVPVTFDGDQQARGRPIAPLLDALRELGVAVDGTGLPFRVRGNGSLAGGTV
AIDASASSQFVSGLLLSAASFTDGLTVQHTGSSLPSAPHIAMTAAMLRQAGVDIDDSTPNRWQVRPGPVAARRWDIEPDL
TNAVAFLSAAVVSGGTVRITGWPRVSVQPADHILAILRQLNAVVIHADSSLEVRGPTGYDGFDVDLRAVGELTPSVAALA
ALASPGSVSRLSGIAHLRGHETDRLAALSTEINRLGGTCRETPDGLVITATPLRPGIWRAYADHRMAMAGAIIGLRVAGV
EVDDIAATTKTLPEFPRLWAEMVGPGQGWGYPQPRSGQRARRATGQGSGG

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; sixth step. [C]

COG id: COG0128

COG function: function code E; 5-enolpyruvylshikimate-3-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EPSP synthase family [H]

Homologues:

Organism=Escherichia coli, GI1787137, Length=418, Percent_Identity=32.5358851674641, Blast_Score=155, Evalue=5e-39,
Organism=Saccharomyces cerevisiae, GI6320332, Length=448, Percent_Identity=29.2410714285714, Blast_Score=134, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001986
- InterPro:   IPR006264
- InterPro:   IPR023193
- InterPro:   IPR013792 [H]

Pfam domain/function: PF00275 EPSP_synthase [H]

EC number: =2.5.1.19 [H]

Molecular weight: Translated: 46426; Mature: 46426

Theoretical pI: Translated: 9.26; Mature: 9.26

Prosite motif: PS00104 EPSP_SYNTHASE_1 ; PS00885 EPSP_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTWPAPTAPTPVRATVTVPGSKSQTNRALVLAALAAAQGRGASTISGALRSRDTELMLD
CCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHH
ALQTLGLRVDGVGSELTVSGRIEPGPGARVDCGLAGTVLRFVPPLAALGSVPVTFDGDQQ
HHHHHCEEEECCCCEEEEEEEECCCCCCEEECCHHHHHHHHHCCHHHHCCCCEEECCCCC
ARGRPIAPLLDALRELGVAVDGTGLPFRVRGNGSLAGGTVAIDASASSQFVSGLLLSAAS
CCCCCHHHHHHHHHHCCEEECCCCCEEEECCCCCCCCCEEEEECCCHHHHHHHHHHHHHH
FTDGLTVQHTGSSLPSAPHIAMTAAMLRQAGVDIDDSTPNRWQVRPGPVAARRWDIEPDL
CCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCEEECCCCCH
TNAVAFLSAAVVSGGTVRITGWPRVSVQPADHILAILRQLNAVVIHADSSLEVRGPTGYD
HHHHHHHHHHHHCCCEEEEECCCCEECCCHHHHHHHHHHCCEEEEEECCCEEECCCCCCC
GFDVDLRAVGELTPSVAALAALASPGSVSRLSGIAHLRGHETDRLAALSTEINRLGGTCR
CCCCHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCC
ETPDGLVITATPLRPGIWRAYADHRMAMAGAIIGLRVAGVEVDDIAATTKTLPEFPRLWA
CCCCCEEEEECCCCCHHHHHHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHCHHHHHHHH
EMVGPGQGWGYPQPRSGQRARRATGQGSGG
HHCCCCCCCCCCCCCCCCHHHHCCCCCCCC
>Mature Secondary Structure
MKTWPAPTAPTPVRATVTVPGSKSQTNRALVLAALAAAQGRGASTISGALRSRDTELMLD
CCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHH
ALQTLGLRVDGVGSELTVSGRIEPGPGARVDCGLAGTVLRFVPPLAALGSVPVTFDGDQQ
HHHHHCEEEECCCCEEEEEEEECCCCCCEEECCHHHHHHHHHCCHHHHCCCCEEECCCCC
ARGRPIAPLLDALRELGVAVDGTGLPFRVRGNGSLAGGTVAIDASASSQFVSGLLLSAAS
CCCCCHHHHHHHHHHCCEEECCCCCEEEECCCCCCCCCEEEEECCCHHHHHHHHHHHHHH
FTDGLTVQHTGSSLPSAPHIAMTAAMLRQAGVDIDDSTPNRWQVRPGPVAARRWDIEPDL
CCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCEEECCCCCH
TNAVAFLSAAVVSGGTVRITGWPRVSVQPADHILAILRQLNAVVIHADSSLEVRGPTGYD
HHHHHHHHHHHHCCCEEEEECCCCEECCCHHHHHHHHHHCCEEEEEECCCEEECCCCCCC
GFDVDLRAVGELTPSVAALAALASPGSVSRLSGIAHLRGHETDRLAALSTEINRLGGTCR
CCCCHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCC
ETPDGLVITATPLRPGIWRAYADHRMAMAGAIIGLRVAGVEVDDIAATTKTLPEFPRLWA
CCCCCEEEEECCCCCHHHHHHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHCHHHHHHHH
EMVGPGQGWGYPQPRSGQRARRATGQGSGG
HHCCCCCCCCCCCCCCCCHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]