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The map label for this gene is adhE2 [C]

Identifier: 121638142

GI number: 121638142

Start: 2510699

End: 2511784

Strand: Direct

Name: adhE2 [C]

Synonym: BCG_2277

Alternate gene names: 121638142

Gene position: 2510699-2511784 (Clockwise)

Preceding gene: 121638136

Following gene: 121638143

Centisome position: 57.39

GC content: 66.57

Gene sequence:

>1086_bases
ATGAGTCAGACGGTGCGCGGTGTGATCGCACGACAAAAGGGCGAACCCGTTGAGCTGGTGAACATTGTCGTCCCGGATCC
CGGACCCGGCGAGGCCGTGGTCGACGTCACCGCCTGCGGGGTATGCCATACCGACCTGACCTACCGCGAGGGCGGCATCA
ACGACGAATACCCTTTTCTGCTCGGACACGAGGCCGCGGGCATCATCGAGGCCGTCGGGCCGGGTGTAACCGCAGTCGAG
CCCGGCGACTTCGTGATCCTGAACTGGCGTGCCGTGTGCGGCCAGTGCCGGGCCTGCAAACGCGGACGGCCCCGCTACTG
CTTCGACACCTTTAACGCCGAACAGAAGATGACGCTGACCGACGGCACCGAGCTCACTGCGGCGTTGGGCATCGGGGCCT
TTGCCGATAAGACGCTGGTGCACTCTGGCCAGTGCACGAAGGTCGATCCGGCTGCCGATCCCGCGGTGGCCGGCCTGCTG
GGTTGCGGGGTCATGGCCGGCCTGGGCGCCGCGATCAACACCGGCGGGGTAACCCGCGACGACACCGTCGCGGTGATCGG
CTGCGGCGGCGTTGGCGATGCCGCGATCGCCGGTGCCGCGCTGGTCGGCGCCAAACGGATCATCGCGGTCGACACCGATG
ACACGAAGCTTGACTGGGCCCGCACCTTCGGCGCCACCCACACCGTCAACGCCCGCGAAGTCGACGTCGTCCAGGCCATC
GGCGGCCTCACGGATGGATTCGGCGCGGACGTGGTGATCGACGCCGTCGGCCGACCGGAAACCTACCAGCAGGCCTTCTA
CGCCCGCGATCTCGCCGGAACCGTTGTGCTGGTGGGTGTTCCGACGCCCGACATGCGCCTGGACATGCCGCTGGTCGACT
TCTTCTCTCACGGCGGTGCGCTGAAGTCGTCGTGGTACGGCGATTGCCTGCCCGAAAGCGACTTCCCCACGCTGATCGAC
CTTTACCTGCAGGGCCGGCTGCCGCTGCAGCGGTTCGTTTCCGAACGCATCGGGCTCGAAGACGTCGAGGAGGCGTTCCA
CAAGATGCATGGCGGCAAGGTATTGCGTTCGGTGGTGATGTTGTGA

Upstream 100 bases:

>100_bases
GTCACCGGCGCGACTCATCTGCGCCGCTCCTCCTCATCGCTTCGCTCTGCATCGTCACCGGCGCGCATGGTCAGCGACGC
TACACCGTAGGTTGGACACC

Downstream 100 bases:

>100_bases
TGGCCGCCATCGAGCGCGTCATCACCCACGGCACCTTCGAACTCGATGGCGGCAGTTGGGAAGTCGACAACAACATCTGG
CTGGTCGGCGACGACTCCGA

Product: putative zinc-dependent alcohol dehydrogenase AdhE2

Products: NA

Alternate protein names: NAD/mycothiol-dependent formaldehyde dehydrogenase; MD-FALDH [H]

Number of amino acids: Translated: 361; Mature: 360

Protein sequence:

>361_residues
MSQTVRGVIARQKGEPVELVNIVVPDPGPGEAVVDVTACGVCHTDLTYREGGINDEYPFLLGHEAAGIIEAVGPGVTAVE
PGDFVILNWRAVCGQCRACKRGRPRYCFDTFNAEQKMTLTDGTELTAALGIGAFADKTLVHSGQCTKVDPAADPAVAGLL
GCGVMAGLGAAINTGGVTRDDTVAVIGCGGVGDAAIAGAALVGAKRIIAVDTDDTKLDWARTFGATHTVNAREVDVVQAI
GGLTDGFGADVVIDAVGRPETYQQAFYARDLAGTVVLVGVPTPDMRLDMPLVDFFSHGGALKSSWYGDCLPESDFPTLID
LYLQGRLPLQRFVSERIGLEDVEEAFHKMHGGKVLRSVVML

Sequences:

>Translated_361_residues
MSQTVRGVIARQKGEPVELVNIVVPDPGPGEAVVDVTACGVCHTDLTYREGGINDEYPFLLGHEAAGIIEAVGPGVTAVE
PGDFVILNWRAVCGQCRACKRGRPRYCFDTFNAEQKMTLTDGTELTAALGIGAFADKTLVHSGQCTKVDPAADPAVAGLL
GCGVMAGLGAAINTGGVTRDDTVAVIGCGGVGDAAIAGAALVGAKRIIAVDTDDTKLDWARTFGATHTVNAREVDVVQAI
GGLTDGFGADVVIDAVGRPETYQQAFYARDLAGTVVLVGVPTPDMRLDMPLVDFFSHGGALKSSWYGDCLPESDFPTLID
LYLQGRLPLQRFVSERIGLEDVEEAFHKMHGGKVLRSVVML
>Mature_360_residues
SQTVRGVIARQKGEPVELVNIVVPDPGPGEAVVDVTACGVCHTDLTYREGGINDEYPFLLGHEAAGIIEAVGPGVTAVEP
GDFVILNWRAVCGQCRACKRGRPRYCFDTFNAEQKMTLTDGTELTAALGIGAFADKTLVHSGQCTKVDPAADPAVAGLLG
CGVMAGLGAAINTGGVTRDDTVAVIGCGGVGDAAIAGAALVGAKRIIAVDTDDTKLDWARTFGATHTVNAREVDVVQAIG
GLTDGFGADVVIDAVGRPETYQQAFYARDLAGTVVLVGVPTPDMRLDMPLVDFFSHGGALKSSWYGDCLPESDFPTLIDL
YLQGRLPLQRFVSERIGLEDVEEAFHKMHGGKVLRSVVML

Specific function: Has High Formaldehyde Dehydrogenase Activity In The Presence Of Glutathione And Catalyzes The Oxidation Of Normal Alcohols In A Reaction That Is Not Gsh-Dependent. In Addition, Hemithiolacetals Other Than Those Formed From Gsh, Including Omega-Thiol Fatty

COG id: COG1062

COG function: function code C; Zn-dependent alcohol dehydrogenases, class III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI34577061, Length=358, Percent_Identity=32.9608938547486, Blast_Score=191, Evalue=7e-49,
Organism=Homo sapiens, GI4501929, Length=358, Percent_Identity=33.2402234636872, Blast_Score=187, Evalue=2e-47,
Organism=Homo sapiens, GI4501933, Length=358, Percent_Identity=31.0055865921788, Blast_Score=179, Evalue=4e-45,
Organism=Homo sapiens, GI156523966, Length=368, Percent_Identity=30.7065217391304, Blast_Score=172, Evalue=5e-43,
Organism=Homo sapiens, GI71565154, Length=369, Percent_Identity=31.4363143631436, Blast_Score=169, Evalue=3e-42,
Organism=Homo sapiens, GI4501939, Length=360, Percent_Identity=30.8333333333333, Blast_Score=165, Evalue=5e-41,
Organism=Homo sapiens, GI71565152, Length=372, Percent_Identity=30.1075268817204, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI262073058, Length=370, Percent_Identity=29.1891891891892, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI71743840, Length=370, Percent_Identity=29.1891891891892, Blast_Score=159, Evalue=3e-39,
Organism=Escherichia coli, GI1786552, Length=366, Percent_Identity=34.1530054644809, Blast_Score=178, Evalue=4e-46,
Organism=Escherichia coli, GI1790045, Length=352, Percent_Identity=25.5681818181818, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI87081918, Length=262, Percent_Identity=30.1526717557252, Blast_Score=98, Evalue=8e-22,
Organism=Escherichia coli, GI1788073, Length=359, Percent_Identity=24.7910863509749, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1788407, Length=342, Percent_Identity=26.3157894736842, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787863, Length=355, Percent_Identity=25.3521126760563, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI87082125, Length=357, Percent_Identity=28.5714285714286, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1786518, Length=286, Percent_Identity=27.972027972028, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1788075, Length=347, Percent_Identity=26.8011527377522, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786825, Length=251, Percent_Identity=29.4820717131474, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI226510992, Length=341, Percent_Identity=24.9266862170088, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1790718, Length=322, Percent_Identity=22.6708074534161, Blast_Score=62, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI25146526, Length=372, Percent_Identity=30.9139784946237, Blast_Score=171, Evalue=6e-43,
Organism=Caenorhabditis elegans, GI71997431, Length=374, Percent_Identity=30.7486631016043, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17565904, Length=342, Percent_Identity=31.5789473684211, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17562876, Length=353, Percent_Identity=26.3456090651558, Blast_Score=103, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI17562582, Length=362, Percent_Identity=26.2430939226519, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17562584, Length=365, Percent_Identity=28.2191780821918, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI71988145, Length=364, Percent_Identity=26.3736263736264, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17562878, Length=345, Percent_Identity=24.6376811594203, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6320033, Length=368, Percent_Identity=33.4239130434783, Blast_Score=187, Evalue=3e-48,
Organism=Saccharomyces cerevisiae, GI6319621, Length=368, Percent_Identity=27.445652173913, Blast_Score=96, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6323729, Length=369, Percent_Identity=26.5582655826558, Blast_Score=89, Evalue=8e-19,
Organism=Saccharomyces cerevisiae, GI6323099, Length=358, Percent_Identity=25.6983240223464, Blast_Score=86, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6324486, Length=366, Percent_Identity=25.4098360655738, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323961, Length=360, Percent_Identity=23.8888888888889, Blast_Score=85, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6322619, Length=386, Percent_Identity=26.4248704663212, Blast_Score=81, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6319955, Length=386, Percent_Identity=26.1658031088083, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6319257, Length=356, Percent_Identity=25.2808988764045, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6319258, Length=296, Percent_Identity=23.6486486486486, Blast_Score=76, Evalue=8e-15,
Organism=Drosophila melanogaster, GI17737895, Length=378, Percent_Identity=32.8042328042328, Blast_Score=175, Evalue=4e-44,
Organism=Drosophila melanogaster, GI17737897, Length=350, Percent_Identity=26.5714285714286, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI17137530, Length=349, Percent_Identity=23.7822349570201, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR017816
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.306 [H]

Molecular weight: Translated: 37889; Mature: 37757

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQTVRGVIARQKGEPVELVNIVVPDPGPGEAVVDVTACGVCHTDLTYREGGINDEYPFL
CCCHHHHHHHHCCCCCEEEEEEEECCCCCCCEEEEEEECCEEECCCEECCCCCCCCCCEE
LGHEAAGIIEAVGPGVTAVEPGDFVILNWRAVCGQCRACKRGRPRYCFDTFNAEQKMTLT
ECCCHHHHHHHCCCCCEEECCCCEEEEEHHHHHHHHHHHHCCCCCEEEECCCCCCEEEEC
DGTELTAALGIGAFADKTLVHSGQCTKVDPAADPAVAGLLGCGVMAGLGAAINTGGVTRD
CCCCCHHHHCCCHHHCCHHEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCC
DTVAVIGCGGVGDAAIAGAALVGAKRIIAVDTDDTKLDWARTFGATHTVNAREVDVVQAI
CCEEEEECCCCCHHHHHHHHHHCCCEEEEECCCCCCCHHHHHCCCCEECCCHHHHHHHHH
GGLTDGFGADVVIDAVGRPETYQQAFYARDLAGTVVLVGVPTPDMRLDMPLVDFFSHGGA
CCCCCCCCCCEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCEECCHHHHHHHCCCC
LKSSWYGDCLPESDFPTLIDLYLQGRLPLQRFVSERIGLEDVEEAFHKMHGGKVLRSVVM
CCCCCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHC
L
C
>Mature Secondary Structure 
SQTVRGVIARQKGEPVELVNIVVPDPGPGEAVVDVTACGVCHTDLTYREGGINDEYPFL
CCHHHHHHHHCCCCCEEEEEEEECCCCCCCEEEEEEECCEEECCCEECCCCCCCCCCEE
LGHEAAGIIEAVGPGVTAVEPGDFVILNWRAVCGQCRACKRGRPRYCFDTFNAEQKMTLT
ECCCHHHHHHHCCCCCEEECCCCEEEEEHHHHHHHHHHHHCCCCCEEEECCCCCCEEEEC
DGTELTAALGIGAFADKTLVHSGQCTKVDPAADPAVAGLLGCGVMAGLGAAINTGGVTRD
CCCCCHHHHCCCHHHCCHHEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCC
DTVAVIGCGGVGDAAIAGAALVGAKRIIAVDTDDTKLDWARTFGATHTVNAREVDVVQAI
CCEEEEECCCCCHHHHHHHHHHCCCEEEEECCCCCCCHHHHHCCCCEECCCHHHHHHHHH
GGLTDGFGADVVIDAVGRPETYQQAFYARDLAGTVVLVGVPTPDMRLDMPLVDFFSHGGA
CCCCCCCCCCEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCEECCHHHHHHHCCCC
LKSSWYGDCLPESDFPTLIDLYLQGRLPLQRFVSERIGLEDVEEAFHKMHGGKVLRSVVM
CCCCCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHC
L
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9346279; 1597190; 9202149 [H]