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The map label for this gene is dop [H]

Identifier: 121637994

GI number: 121637994

Start: 2350999

End: 2352606

Strand: Reverse

Name: dop [H]

Synonym: BCG_2129c

Alternate gene names: 121637994

Gene position: 2352606-2350999 (Counterclockwise)

Preceding gene: 121637997

Following gene: 121637993

Centisome position: 53.78

GC content: 66.98

Gene sequence:

>1608_bases
ATGTTCTGGGTCGGCGGGCGTTGCTTGATGCCCGCCTCCTCAGCGGCCCGCTGCGCGGCCCGCATCGTCGGCGGGCCTAG
GCTCTACGGCATGCAGCGGATTATCGGAACGGAGGTCGAGTACGGCATTTCCTCGCCGTCGGACCCGACCGCCAACCCGA
TCCTCACCTCGACGCAGGCGGTGCTGGCATACGCCGCCGCCGCCGGCATTCAGCGTGCCAAACGCACCCGTTGGGACTAC
GAGGTGGAATCGCCGCTGCGCGACGCCCGGGGCTTCGATTTGAGTCGCTCGGCCGGGCCGCCGCCGGTGGTCGACGCCGA
CGAGGTCGGCGCGGCCAACATGATCCTGACCAACGGGGCGCGGCTGTATGTCGACCACGCGCACCCGGAATACTCCGCGC
CCGAATGCACCGACCCGCTGGACGCAGTGATCTGGGACAAGGCGGGCGAACGCGTGATGGAGGCCGCTGCCCGCCATGTC
GCCAGCGTGCCCGGGGCCGCGAAACTGCAGCTGTACAAGAACAACGTCGACGGCAAGGGAGCCTCCTACGGGTCGCACGA
GAACTACCTGATGTCGCGGCAGACACCGTTCTCGGCGATCATCACCGGGCTGACCCCCTTTCTGGTATCCCGGCAGGTGG
TGACCGGCTCGGGCCGGGTCGGCATCGGGCCCTCGGGTGATGAGCCCGGCTTCCAGCTATCCCAGCGTTCGGACTACATC
GAGGTCGAGGTAGGGCTGGAAACAACGCTCAAGCGCGGCATCATCAACACCCGCGACGAACCGCACGCCGACGCCGACAG
GTACCGCCGGCTGCACGTCATCATCGGCGACGCCAACCTTGCCGAGACGTCGACCTATCTGAAGTTGGGTACCACGGCGC
TGGTGCTCGACCTGATCGAAGAAGGACCAGCCCACGCAATAGATCTGACCGACCTGGCGCTGGCCCGCCCGGTACATGCG
GTGCACGCAATCTCCCGCGATCCGTCGCTGCGAGCGACCGTTGCGCTGGCCGACGGCCGGGAACTGACCGGTCTTGCGCT
GCAACGGATCTACCTGGACCGAGTGGCTAAGTTGGTGGATAGCCGCGACCCGGACCCGCGGGCGGCCGACATCGTGGAAA
CCTGGGCACACGTGCTGGATCAGCTCGAGCGTGACCCGATGGATTGCGCGGAGCTGCTGGACTGGCCGGCCAAACTGCGG
CTGCTCGACGGTTTCCGGCAGCGGGAGAACCTGAGCTGGTCGGCGCCCCGGCTGCACCTCGTCGACCTGCAGTACTCCGA
TGTCCGGCTGGACAAGGGCCTGTACAACCGGCTGGTCGCGCGCGGCTCGATGAAGCGTTTAGTCACCGAACACCAGGTGC
TGAGTGCGGTGGAGAACCCGCCGACCGACACCCGCGCGTATTTCCGCGGCGAATGCCTGCGCCGGTTCGGGGCTGATATC
GCCGCGGCTAGCTGGGACTCGGTGATCTTCGACCTGGGCGGCGACTCGCTGGTTCGCATCCCGACGCTGGAGCCGTTGCG
GGGTAGTAAGGCGCATGTTGGTGCGTTGCTGGATTCGGTGGACAGTGCCGTGGAGCTGGTAGAGCAACTGACCGCTGAGC
CTCGCTAA

Upstream 100 bases:

>100_bases
CTGCTCGGGCCGCCGGGGGGCGACGGACGGAAAGGCTCATGCCGGCCTACCCTAGCGCCGGGCCCTGCTTGATTGTCCAC
CTCCTGCGCGGCCCGCTCCG

Downstream 100 bases:

>100_bases
GCCTGGTTAGGCCGGGAAACGTTGGTGTTGACCGGTAGGGTAGAGAAAGACAGCGGGCGTGTGACGCGCACCCGCGGCCT
TTTGAAAGGCCATGAGGAAG

Product: hypothetical protein

Products: NA

Alternate protein names: Deamidase of protein Pup [H]

Number of amino acids: Translated: 535; Mature: 535

Protein sequence:

>535_residues
MFWVGGRCLMPASSAARCAARIVGGPRLYGMQRIIGTEVEYGISSPSDPTANPILTSTQAVLAYAAAAGIQRAKRTRWDY
EVESPLRDARGFDLSRSAGPPPVVDADEVGAANMILTNGARLYVDHAHPEYSAPECTDPLDAVIWDKAGERVMEAAARHV
ASVPGAAKLQLYKNNVDGKGASYGSHENYLMSRQTPFSAIITGLTPFLVSRQVVTGSGRVGIGPSGDEPGFQLSQRSDYI
EVEVGLETTLKRGIINTRDEPHADADRYRRLHVIIGDANLAETSTYLKLGTTALVLDLIEEGPAHAIDLTDLALARPVHA
VHAISRDPSLRATVALADGRELTGLALQRIYLDRVAKLVDSRDPDPRAADIVETWAHVLDQLERDPMDCAELLDWPAKLR
LLDGFRQRENLSWSAPRLHLVDLQYSDVRLDKGLYNRLVARGSMKRLVTEHQVLSAVENPPTDTRAYFRGECLRRFGADI
AAASWDSVIFDLGGDSLVRIPTLEPLRGSKAHVGALLDSVDSAVELVEQLTAEPR

Sequences:

>Translated_535_residues
MFWVGGRCLMPASSAARCAARIVGGPRLYGMQRIIGTEVEYGISSPSDPTANPILTSTQAVLAYAAAAGIQRAKRTRWDY
EVESPLRDARGFDLSRSAGPPPVVDADEVGAANMILTNGARLYVDHAHPEYSAPECTDPLDAVIWDKAGERVMEAAARHV
ASVPGAAKLQLYKNNVDGKGASYGSHENYLMSRQTPFSAIITGLTPFLVSRQVVTGSGRVGIGPSGDEPGFQLSQRSDYI
EVEVGLETTLKRGIINTRDEPHADADRYRRLHVIIGDANLAETSTYLKLGTTALVLDLIEEGPAHAIDLTDLALARPVHA
VHAISRDPSLRATVALADGRELTGLALQRIYLDRVAKLVDSRDPDPRAADIVETWAHVLDQLERDPMDCAELLDWPAKLR
LLDGFRQRENLSWSAPRLHLVDLQYSDVRLDKGLYNRLVARGSMKRLVTEHQVLSAVENPPTDTRAYFRGECLRRFGADI
AAASWDSVIFDLGGDSLVRIPTLEPLRGSKAHVGALLDSVDSAVELVEQLTAEPR
>Mature_535_residues
MFWVGGRCLMPASSAARCAARIVGGPRLYGMQRIIGTEVEYGISSPSDPTANPILTSTQAVLAYAAAAGIQRAKRTRWDY
EVESPLRDARGFDLSRSAGPPPVVDADEVGAANMILTNGARLYVDHAHPEYSAPECTDPLDAVIWDKAGERVMEAAARHV
ASVPGAAKLQLYKNNVDGKGASYGSHENYLMSRQTPFSAIITGLTPFLVSRQVVTGSGRVGIGPSGDEPGFQLSQRSDYI
EVEVGLETTLKRGIINTRDEPHADADRYRRLHVIIGDANLAETSTYLKLGTTALVLDLIEEGPAHAIDLTDLALARPVHA
VHAISRDPSLRATVALADGRELTGLALQRIYLDRVAKLVDSRDPDPRAADIVETWAHVLDQLERDPMDCAELLDWPAKLR
LLDGFRQRENLSWSAPRLHLVDLQYSDVRLDKGLYNRLVARGSMKRLVTEHQVLSAVENPPTDTRAYFRGECLRRFGADI
AAASWDSVIFDLGGDSLVRIPTLEPLRGSKAHVGALLDSVDSAVELVEQLTAEPR

Specific function: Specifically catalyzes the deamidation of the C-terminal glutamine of the prokaryotic ubiquitin-like protein Pup to glutamate, thereby rendering Pup competent for conjugation. Also displays depupylase (DPUP) activity, removing conjugated Pup from target p

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Pup ligase/Pup deamidase family. Pup deamidase subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022366
- InterPro:   IPR004347 [H]

Pfam domain/function: PF03136 DUF245 [H]

EC number: NA

Molecular weight: Translated: 58317; Mature: 58317

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFWVGGRCLMPASSAARCAARIVGGPRLYGMQRIIGTEVEYGISSPSDPTANPILTSTQA
CEEECCEEECCCCHHHHHHHHHCCCCHHHHHHHHHCCCHHCCCCCCCCCCCCCCHHHHHH
VLAYAAAAGIQRAKRTRWDYEVESPLRDARGFDLSRSAGPPPVVDADEVGAANMILTNGA
HHHHHHHHHHHHHHHCCCCCCHHCHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEECCC
RLYVDHAHPEYSAPECTDPLDAVIWDKAGERVMEAAARHVASVPGAAKLQLYKNNVDGKG
EEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCC
ASYGSHENYLMSRQTPFSAIITGLTPFLVSRQVVTGSGRVGIGPSGDEPGFQLSQRSDYI
CCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCEECCCCCEE
EVEVGLETTLKRGIINTRDEPHADADRYRRLHVIIGDANLAETSTYLKLGTTALVLDLIE
EEEECHHHHHHHCCCCCCCCCCCCHHHCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHH
EGPAHAIDLTDLALARPVHAVHAISRDPSLRATVALADGRELTGLALQRIYLDRVAKLVD
CCCCCEEEHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
SRDPDPRAADIVETWAHVLDQLERDPMDCAELLDWPAKLRLLDGFRQRENLSWSAPRLHL
CCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCEEE
VDLQYSDVRLDKGLYNRLVARGSMKRLVTEHQVLSAVENPPTDTRAYFRGECLRRFGADI
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCH
AAASWDSVIFDLGGDSLVRIPTLEPLRGSKAHVGALLDSVDSAVELVEQLTAEPR
HHCCCCCEEEECCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MFWVGGRCLMPASSAARCAARIVGGPRLYGMQRIIGTEVEYGISSPSDPTANPILTSTQA
CEEECCEEECCCCHHHHHHHHHCCCCHHHHHHHHHCCCHHCCCCCCCCCCCCCCHHHHHH
VLAYAAAAGIQRAKRTRWDYEVESPLRDARGFDLSRSAGPPPVVDADEVGAANMILTNGA
HHHHHHHHHHHHHHHCCCCCCHHCHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEECCC
RLYVDHAHPEYSAPECTDPLDAVIWDKAGERVMEAAARHVASVPGAAKLQLYKNNVDGKG
EEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCC
ASYGSHENYLMSRQTPFSAIITGLTPFLVSRQVVTGSGRVGIGPSGDEPGFQLSQRSDYI
CCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCEECCCCCEE
EVEVGLETTLKRGIINTRDEPHADADRYRRLHVIIGDANLAETSTYLKLGTTALVLDLIE
EEEECHHHHHHHCCCCCCCCCCCCHHHCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHH
EGPAHAIDLTDLALARPVHAVHAISRDPSLRATVALADGRELTGLALQRIYLDRVAKLVD
CCCCCEEEHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
SRDPDPRAADIVETWAHVLDQLERDPMDCAELLDWPAKLRLLDGFRQRENLSWSAPRLHL
CCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCEEE
VDLQYSDVRLDKGLYNRLVARGSMKRLVTEHQVLSAVENPPTDTRAYFRGECLRRFGADI
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCH
AAASWDSVIFDLGGDSLVRIPTLEPLRGSKAHVGALLDSVDSAVELVEQLTAEPR
HHCCCCCEEEECCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]