Click here to switch to the map view.

The map label for this gene is pykA [H]

Identifier: 121637524

GI number: 121637524

Start: 1821436

End: 1822854

Strand: Direct

Name: pykA [H]

Synonym: BCG_1655

Alternate gene names: 121637524

Gene position: 1821436-1822854 (Clockwise)

Preceding gene: 121637523

Following gene: 121637525

Centisome position: 41.64

GC content: 66.31

Gene sequence:

>1419_bases
GTGACGAGACGCGGGAAAATCGTCTGCACTCTCGGGCCGGCCACCCAGCGGGACGACCTGGTCAGAGCGCTGGTCGAGGC
CGGAATGGACGTCGCCCGAATGAACTTCAGCCACGGCGACTACGACGATCACAAGGTCGCCTATGAGCGGGTCCGGGTAG
CCTCCGACGCCACCGGGCGCGCGGTCGGCGTGCTCGCCGACCTGCAGGGCCCGAAGATCAGGTTGGGACGCTTCGCCTCC
GGGGCCACCCACTGGGCCGAAGGCGAAACCGTCCGGATCACCGTGGGCGCCTGCGAGGGCAGCCACGATCGGGTGTCCAC
CACCTACAAGCGGCTAGCCCAGGACGCGGTGGCCGGTGACCGGGTGCTGGTCGACGACGGCAAAGTCGCATTGGTGGTCG
ACGCCGTCGAGGGCGACGACGTGGTCTGCACCGTCGTCGAAGGCGGCCCGGTCAGCGACAACAAGGGCATCTCGTTGCCC
GGAATGAACGTGACCGCGCCGGCCCTGTCGGAGAAGGACATCGAGGATCTCACGTTCGCGCTGAACCTCGGCGTCGACAT
GGTGGCGCTTTCCTTCGTCCGCTCCCCGGCCGATGTCGAACTGGTCCACGAGGTGATGGATCGGATCGGGCGACGGGTGC
CGGTGATCGCCAAGCTGGAGAAGCCGGAAGCCATCGACAATCTCGAAGCGATCGTGCTGGCGTTCGACGCCGTCATGGTC
GCTCGGGGCGACCTAGGTGTTGAGCTGCCGCTCGAAGAGGTCCCGCTGGTACAGAAGCGAGCCATCCAGATGGCCCGGGA
GAACGCCAAGCCGGTCATTGTGGCGACCCAGATGCTCGACTCGATGATCGAGAACTCGCGGCCGACCCGAGCTGAGGCCT
CCGACGTCGCCAACGCGGTGCTCGATGGCGCCGACGCGCTGATGCTGTCCGGGGAAACCTCGGTAGGGAAGTACCCCCTT
GCTGCGGTCCGGACAATGTCGCGCATCATCTGCGCGGTCGAGGAGAACTCCACGGCCGCACCGCCGTTGACACACATTCC
CCGGACCAAGCGTGGGGTCATCTCGTATGCGGCCCGTGACATCGGCGAACGACTCGACGCCAAGGCCTTGGTGGCCTTCA
CTCAGTCCGGTGATACCGTGCGGCGACTGGCCCGCCTGCATACCCCGCTGCCGCTGCTGGCCTTCACCGCGTGGCCCGAG
GTGCGCAGCCAACTGGCGATGACCTGGGGCACCGAGACGTTCATCGTGCCGAAGATGCAGTCCACCGATGGCATGATCCG
CCAGGTCGACAAATCGCTGCTCGAACTCGCCCGCTACAAGCGTGGTGACTTGGTGGTCATCGTCGCGGGTGCGCCGCCAG
GCACAGTGGGTTCGACCAACCTGATCCACGTGCACCGGATCGGGGAAGATGACGTCTAG

Upstream 100 bases:

>100_bases
TACCCGCGGTCTGGTTGGCTGGGCTGCCCGCGGTGGTGTTGACCGGTGTGCCGACCCGGCGGTGCCGGCCCTACCGCCGT
CGCGACTATGCTGAGTCGTC

Downstream 100 bases:

>100_bases
CCGGGTCGTGCCGGACGGTAAACCCATGTCCGACTTCGATGAACTACTGGCGGTATTGGACCTCAACGCCGTCGCAAGCG
ACCTGTTCACCGGATCCCAC

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MTRRGKIVCTLGPATQRDDLVRALVEAGMDVARMNFSHGDYDDHKVAYERVRVASDATGRAVGVLADLQGPKIRLGRFAS
GATHWAEGETVRITVGACEGSHDRVSTTYKRLAQDAVAGDRVLVDDGKVALVVDAVEGDDVVCTVVEGGPVSDNKGISLP
GMNVTAPALSEKDIEDLTFALNLGVDMVALSFVRSPADVELVHEVMDRIGRRVPVIAKLEKPEAIDNLEAIVLAFDAVMV
ARGDLGVELPLEEVPLVQKRAIQMARENAKPVIVATQMLDSMIENSRPTRAEASDVANAVLDGADALMLSGETSVGKYPL
AAVRTMSRIICAVEENSTAAPPLTHIPRTKRGVISYAARDIGERLDAKALVAFTQSGDTVRRLARLHTPLPLLAFTAWPE
VRSQLAMTWGTETFIVPKMQSTDGMIRQVDKSLLELARYKRGDLVVIVAGAPPGTVGSTNLIHVHRIGEDDV

Sequences:

>Translated_472_residues
MTRRGKIVCTLGPATQRDDLVRALVEAGMDVARMNFSHGDYDDHKVAYERVRVASDATGRAVGVLADLQGPKIRLGRFAS
GATHWAEGETVRITVGACEGSHDRVSTTYKRLAQDAVAGDRVLVDDGKVALVVDAVEGDDVVCTVVEGGPVSDNKGISLP
GMNVTAPALSEKDIEDLTFALNLGVDMVALSFVRSPADVELVHEVMDRIGRRVPVIAKLEKPEAIDNLEAIVLAFDAVMV
ARGDLGVELPLEEVPLVQKRAIQMARENAKPVIVATQMLDSMIENSRPTRAEASDVANAVLDGADALMLSGETSVGKYPL
AAVRTMSRIICAVEENSTAAPPLTHIPRTKRGVISYAARDIGERLDAKALVAFTQSGDTVRRLARLHTPLPLLAFTAWPE
VRSQLAMTWGTETFIVPKMQSTDGMIRQVDKSLLELARYKRGDLVVIVAGAPPGTVGSTNLIHVHRIGEDDV
>Mature_471_residues
TRRGKIVCTLGPATQRDDLVRALVEAGMDVARMNFSHGDYDDHKVAYERVRVASDATGRAVGVLADLQGPKIRLGRFASG
ATHWAEGETVRITVGACEGSHDRVSTTYKRLAQDAVAGDRVLVDDGKVALVVDAVEGDDVVCTVVEGGPVSDNKGISLPG
MNVTAPALSEKDIEDLTFALNLGVDMVALSFVRSPADVELVHEVMDRIGRRVPVIAKLEKPEAIDNLEAIVLAFDAVMVA
RGDLGVELPLEEVPLVQKRAIQMARENAKPVIVATQMLDSMIENSRPTRAEASDVANAVLDGADALMLSGETSVGKYPLA
AVRTMSRIICAVEENSTAAPPLTHIPRTKRGVISYAARDIGERLDAKALVAFTQSGDTVRRLARLHTPLPLLAFTAWPEV
RSQLAMTWGTETFIVPKMQSTDGMIRQVDKSLLELARYKRGDLVVIVAGAPPGTVGSTNLIHVHRIGEDDV

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286418, Length=489, Percent_Identity=36.8098159509202, Blast_Score=281, Evalue=7e-76,
Organism=Homo sapiens, GI33286422, Length=486, Percent_Identity=36.4197530864198, Blast_Score=280, Evalue=2e-75,
Organism=Homo sapiens, GI33286420, Length=486, Percent_Identity=36.4197530864198, Blast_Score=280, Evalue=2e-75,
Organism=Homo sapiens, GI10835121, Length=492, Percent_Identity=35.5691056910569, Blast_Score=261, Evalue=1e-69,
Organism=Homo sapiens, GI32967597, Length=492, Percent_Identity=35.5691056910569, Blast_Score=261, Evalue=1e-69,
Organism=Homo sapiens, GI310128732, Length=287, Percent_Identity=37.2822299651568, Blast_Score=186, Evalue=4e-47,
Organism=Homo sapiens, GI310128730, Length=287, Percent_Identity=37.2822299651568, Blast_Score=186, Evalue=4e-47,
Organism=Homo sapiens, GI310128736, Length=235, Percent_Identity=34.8936170212766, Blast_Score=146, Evalue=4e-35,
Organism=Homo sapiens, GI310128734, Length=235, Percent_Identity=34.8936170212766, Blast_Score=146, Evalue=4e-35,
Organism=Homo sapiens, GI310128738, Length=216, Percent_Identity=35.1851851851852, Blast_Score=129, Evalue=9e-30,
Organism=Escherichia coli, GI1787965, Length=470, Percent_Identity=40, Blast_Score=333, Evalue=1e-92,
Organism=Escherichia coli, GI1788160, Length=476, Percent_Identity=38.655462184874, Blast_Score=287, Evalue=1e-78,
Organism=Caenorhabditis elegans, GI17544584, Length=490, Percent_Identity=35.3061224489796, Blast_Score=287, Evalue=7e-78,
Organism=Caenorhabditis elegans, GI17506829, Length=485, Percent_Identity=36.0824742268041, Blast_Score=269, Evalue=2e-72,
Organism=Caenorhabditis elegans, GI71984406, Length=485, Percent_Identity=36.0824742268041, Blast_Score=269, Evalue=2e-72,
Organism=Caenorhabditis elegans, GI71984413, Length=485, Percent_Identity=36.0824742268041, Blast_Score=269, Evalue=3e-72,
Organism=Caenorhabditis elegans, GI17506831, Length=337, Percent_Identity=43.026706231454, Blast_Score=268, Evalue=5e-72,
Organism=Saccharomyces cerevisiae, GI6319279, Length=486, Percent_Identity=34.9794238683128, Blast_Score=260, Evalue=4e-70,
Organism=Saccharomyces cerevisiae, GI6324923, Length=487, Percent_Identity=33.6755646817248, Blast_Score=241, Evalue=2e-64,
Organism=Drosophila melanogaster, GI24648964, Length=490, Percent_Identity=36.530612244898, Blast_Score=278, Evalue=6e-75,
Organism=Drosophila melanogaster, GI28571814, Length=490, Percent_Identity=36.530612244898, Blast_Score=278, Evalue=6e-75,
Organism=Drosophila melanogaster, GI24648966, Length=460, Percent_Identity=34.7826086956522, Blast_Score=255, Evalue=4e-68,
Organism=Drosophila melanogaster, GI24581235, Length=405, Percent_Identity=29.3827160493827, Blast_Score=204, Evalue=1e-52,
Organism=Drosophila melanogaster, GI24646914, Length=288, Percent_Identity=34.0277777777778, Blast_Score=151, Evalue=1e-36,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 50700; Mature: 50569

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRRGKIVCTLGPATQRDDLVRALVEAGMDVARMNFSHGDYDDHKVAYERVRVASDATGR
CCCCCCEEEEECCCCCHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHCCCCCCC
AVGVLADLQGPKIRLGRFASGATHWAEGETVRITVGACEGSHDRVSTTYKRLAQDAVAGD
EEEEEEECCCCCEEEECCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHCCC
RVLVDDGKVALVVDAVEGDDVVCTVVEGGPVSDNKGISLPGMNVTAPALSEKDIEDLTFA
EEEEECCCEEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHEEE
LNLGVDMVALSFVRSPADVELVHEVMDRIGRRVPVIAKLEKPEAIDNLEAIVLAFDAVMV
ECCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHE
ARGDLGVELPLEEVPLVQKRAIQMARENAKPVIVATQMLDSMIENSRPTRAEASDVANAV
ECCCCCCCCCHHHCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCCCCHHHHHHHHH
LDGADALMLSGETSVGKYPLAAVRTMSRIICAVEENSTAAPPLTHIPRTKRGVISYAARD
HCCCCEEEEECCCCCCCCCHHHHHHHHHHEEEEECCCCCCCCCCCCCCCHHHHHHHHHHH
IGERLDAKALVAFTQSGDTVRRLARLHTPLPLLAFTAWPEVRSQLAMTWGTETFIVPKMQ
HHHHCCCEEEEEEECCCHHHHHHHHHCCCCCHHHEECCHHHHHHHHHCCCCCEEEEECCC
STDGMIRQVDKSLLELARYKRGDLVVIVAGAPPGTVGSTNLIHVHRIGEDDV
CCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCC
>Mature Secondary Structure 
TRRGKIVCTLGPATQRDDLVRALVEAGMDVARMNFSHGDYDDHKVAYERVRVASDATGR
CCCCCEEEEECCCCCHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHCCCCCCC
AVGVLADLQGPKIRLGRFASGATHWAEGETVRITVGACEGSHDRVSTTYKRLAQDAVAGD
EEEEEEECCCCCEEEECCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHCCC
RVLVDDGKVALVVDAVEGDDVVCTVVEGGPVSDNKGISLPGMNVTAPALSEKDIEDLTFA
EEEEECCCEEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHEEE
LNLGVDMVALSFVRSPADVELVHEVMDRIGRRVPVIAKLEKPEAIDNLEAIVLAFDAVMV
ECCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHE
ARGDLGVELPLEEVPLVQKRAIQMARENAKPVIVATQMLDSMIENSRPTRAEASDVANAV
ECCCCCCCCCHHHCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCCCCHHHHHHHHH
LDGADALMLSGETSVGKYPLAAVRTMSRIICAVEENSTAAPPLTHIPRTKRGVISYAARD
HCCCCEEEEECCCCCCCCCHHHHHHHHHHEEEEECCCCCCCCCCCCCCCHHHHHHHHHHH
IGERLDAKALVAFTQSGDTVRRLARLHTPLPLLAFTAWPEVRSQLAMTWGTETFIVPKMQ
HHHHCCCEEEEEEECCCHHHHHHHHHCCCCCHHHEECCHHHHHHHHHCCCCCEEEEECCC
STDGMIRQVDKSLLELARYKRGDLVVIVAGAPPGTVGSTNLIHVHRIGEDDV
CCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA