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The map label for this gene is bioB [H]

Identifier: 121637496

GI number: 121637496

Start: 1795531

End: 1796580

Strand: Direct

Name: bioB [H]

Synonym: BCG_1627

Alternate gene names: 121637496

Gene position: 1795531-1796580 (Clockwise)

Preceding gene: 121637493

Following gene: 121637497

Centisome position: 41.05

GC content: 67.43

Gene sequence:

>1050_bases
GTGACGCAAGCGGCAACGCGACCGACGAACGACGCCGGCCAGGATGGCGGGAACAACTCGGACATTCTGGTGGTTGCCCG
CCAACAGGTGCTGCAGCGCGGTGAGGGCCTGAACCAGGACCAGGTGCTGGCGGTGCTGCAGCTACCCGACGACCGGCTCG
AGGAGCTGTTGGCGCTGGCCCACGAGGTGCGGATGCGCTGGTGCGGACCCGAGGTCGAGGTCGAAGGCATCATCAGCCTG
AAAACCGGTGGCTGCCCGGAGGATTGCCATTTCTGCTCGCAATCGGGGCTGTTCGCCTCCCCGGTGCGCAGCGCCTGGCT
GGACATACCCAGCCTGGTCGAGGCGGCCAAACAGACCGCCAAGTCCGGCGCCACCGAGTTCTGCATCGTGGCCGCGGTGC
GCGGACCCGACGAGCGATTGATGGCCCAGGTCGCGGCCGGCATCGAGGCGATTCGCAACGAAGTCGAGATCAACATCGCC
TGCTCCCTAGGGATGCTGACCGCCGAGCAAGTGGACCAACTGGCGGCGAGGGGGGTGCATCGCTACAACCACAACCTCGA
AACGGCGCGCTCGTTCTTCGCCAACGTCGTCACCACCCACACCTGGGAAGAGCGCTGGCAGACGCTATCGATGGTGCGTG
ACGCGGGCATGGAGGTTTGCTGCGGCGGCATCCTCGGCATGGGGGAGACGCTGCAGCAGCGCGCGGAATTCGCCGCCGAG
CTTGCCGAGCTGGGCCCCGACGAGGTCCCGCTGAACTTCCTCAACCCGCGGCCCGGTACCCCGTTCGCCGACCTGGAGGT
AATGCCGGTCGGTGACGCGCTCAAGGCGGTGGCCGCCTTCCGGTTGGCGTTACCGCGCACCATGCTGCGGTTCGCCGGTG
GCCGCGAGATCACCCTGGGTGACCTCGGCGCCAAGCGAGGCATCCTGGGCGGCATCAACGCCGTGATCGTCGGCAACTAC
CTGACCACCCTCGGCCGGCCCGCGGAAGCCGACCTGGAACTGCTCGACGAGCTACAGATGCCGCTGAAGGCACTCAACGC
CAGCCTGTAA

Upstream 100 bases:

>100_bases
CCGCTACCGTAGCCTGAACACCGTTTCATTCAGGCCGCCGAGCAGGCGGCGGATGGGTTCCGCGCGTGCGGAGATGACGA
AGGATGCAGGGGAGTACCTG

Downstream 100 bases:

>100_bases
ATGGTGGAAATCGTGGCTGGAAAACAACGCGCTCCGGTCGCTGCCGGCGTGTACAACGTGTACACCGGGGAACTGGCGGA
TACGGCCACGCCGACAGCGG

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 349; Mature: 348

Protein sequence:

>349_residues
MTQAATRPTNDAGQDGGNNSDILVVARQQVLQRGEGLNQDQVLAVLQLPDDRLEELLALAHEVRMRWCGPEVEVEGIISL
KTGGCPEDCHFCSQSGLFASPVRSAWLDIPSLVEAAKQTAKSGATEFCIVAAVRGPDERLMAQVAAGIEAIRNEVEINIA
CSLGMLTAEQVDQLAARGVHRYNHNLETARSFFANVVTTHTWEERWQTLSMVRDAGMEVCCGGILGMGETLQQRAEFAAE
LAELGPDEVPLNFLNPRPGTPFADLEVMPVGDALKAVAAFRLALPRTMLRFAGGREITLGDLGAKRGILGGINAVIVGNY
LTTLGRPAEADLELLDELQMPLKALNASL

Sequences:

>Translated_349_residues
MTQAATRPTNDAGQDGGNNSDILVVARQQVLQRGEGLNQDQVLAVLQLPDDRLEELLALAHEVRMRWCGPEVEVEGIISL
KTGGCPEDCHFCSQSGLFASPVRSAWLDIPSLVEAAKQTAKSGATEFCIVAAVRGPDERLMAQVAAGIEAIRNEVEINIA
CSLGMLTAEQVDQLAARGVHRYNHNLETARSFFANVVTTHTWEERWQTLSMVRDAGMEVCCGGILGMGETLQQRAEFAAE
LAELGPDEVPLNFLNPRPGTPFADLEVMPVGDALKAVAAFRLALPRTMLRFAGGREITLGDLGAKRGILGGINAVIVGNY
LTTLGRPAEADLELLDELQMPLKALNASL
>Mature_348_residues
TQAATRPTNDAGQDGGNNSDILVVARQQVLQRGEGLNQDQVLAVLQLPDDRLEELLALAHEVRMRWCGPEVEVEGIISLK
TGGCPEDCHFCSQSGLFASPVRSAWLDIPSLVEAAKQTAKSGATEFCIVAAVRGPDERLMAQVAAGIEAIRNEVEINIAC
SLGMLTAEQVDQLAARGVHRYNHNLETARSFFANVVTTHTWEERWQTLSMVRDAGMEVCCGGILGMGETLQQRAEFAAEL
AELGPDEVPLNFLNPRPGTPFADLEVMPVGDALKAVAAFRLALPRTMLRFAGGREITLGDLGAKRGILGGINAVIVGNYL
TTLGRPAEADLELLDELQMPLKALNASL

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786992, Length=303, Percent_Identity=36.6336633663366, Blast_Score=191, Evalue=7e-50,
Organism=Saccharomyces cerevisiae, GI6321725, Length=305, Percent_Identity=28.5245901639344, Blast_Score=148, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 37550; Mature: 37419

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQAATRPTNDAGQDGGNNSDILVVARQQVLQRGEGLNQDQVLAVLQLPDDRLEELLALA
CCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHCCCCCHHHEEEEEECCHHHHHHHHHHH
HEVRMRWCGPEVEVEGIISLKTGGCPEDCHFCSQSGLFASPVRSAWLDIPSLVEAAKQTA
HHHHHHHCCCCEEEEEEEEECCCCCCHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHH
KSGATEFCIVAAVRGPDERLMAQVAAGIEAIRNEVEINIACSLGMLTAEQVDQLAARGVH
HCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHEEEEEEEECCHHHHHHHHHHHHHHHH
RYNHNLETARSFFANVVTTHTWEERWQTLSMVRDAGMEVCCGGILGMGETLQQRAEFAAE
HHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHH
LAELGPDEVPLNFLNPRPGTPFADLEVMPVGDALKAVAAFRLALPRTMLRFAGGREITLG
HHHCCCCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCEEEEC
DLGAKRGILGGINAVIVGNYLTTLGRPAEADLELLDELQMPLKALNASL
CCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TQAATRPTNDAGQDGGNNSDILVVARQQVLQRGEGLNQDQVLAVLQLPDDRLEELLALA
CCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHCCCCCHHHEEEEEECCHHHHHHHHHHH
HEVRMRWCGPEVEVEGIISLKTGGCPEDCHFCSQSGLFASPVRSAWLDIPSLVEAAKQTA
HHHHHHHCCCCEEEEEEEEECCCCCCHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHH
KSGATEFCIVAAVRGPDERLMAQVAAGIEAIRNEVEINIACSLGMLTAEQVDQLAARGVH
HCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHEEEEEEEECCHHHHHHHHHHHHHHHH
RYNHNLETARSFFANVVTTHTWEERWQTLSMVRDAGMEVCCGGILGMGETLQQRAEFAAE
HHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHH
LAELGPDEVPLNFLNPRPGTPFADLEVMPVGDALKAVAAFRLALPRTMLRFAGGREITLG
HHHCCCCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCEEEEC
DLGAKRGILGGINAVIVGNYLTTLGRPAEADLELLDELQMPLKALNASL
CCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA