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The map label for this gene is fmt

Identifier: 121637336

GI number: 121637336

Start: 1606954

End: 1607892

Strand: Direct

Name: fmt

Synonym: BCG_1467

Alternate gene names: 121637336

Gene position: 1606954-1607892 (Clockwise)

Preceding gene: 121637334

Following gene: 121637337

Centisome position: 36.73

GC content: 69.86

Gene sequence:

>939_bases
GTGCGCCTTGTCTTTGCCGGCACCCCCGAACCCGCGCTGGCCTCGCTGCGCAGGCTCATCGAATCGCCCAGTCACGACGT
GATCGCCGTGTTGACCCGTCCGGATGCCGCCTCCGGCCGGCGGGGCAAGCCGCAGCCGTCACCGGTGGCCCGTGAGGCGG
CAGAGCGCGGCATTCCGGTGCTGCGGCCATCGCGACCGAACTCGGCAGAGTTCGTCGCCGAACTGTCGGATCTGGCGCCA
GAGTGCTGCGCCGTGGTTGCCTACGGAGCCCTGCTCGGCGGTCCCTTGCTGGCCGTGCCGCCGCATGGCTGGGTCAACCT
GCACTTCTCGCTGCTGCCGGCCTGGCGTGGCGCGGCGCCGGTGCAGGCCGCCATCGCCGCGGGAGACACGATCACCGGAG
CCACGACGTTCCAGATTGAGCCAAGCCTGGACTCGGGACCGATATACGGTGTCGTCACCGAGGTGATCCAGCCGACCGAC
ACCGCGGGCGATCTACTTAAGCGACTGGCGGTATCGGGGGCAGCGCTGCTATCGACCACGCTGGATGGCATCGCCGATCA
GCGGCTGACGCCGCGGCCGCAACCGGCAGACGGGGTCAGCGTGGCGCCGAAAATCACCGTAGCGAATGCCCGGGTGCGAT
GGGACTTGCCGGCGGCGGTCGTGGAGCGGCGGATCCGCGCCGTCACTCCCAACCCCGGCGCCTGGACGCTCATCGGTGAC
TTACGGGTCAAACTTGGACCGGTGCACCTCGACGCCGCTCACCGGCCATCGAAGCCCTTGCCGCCCGGTGGAATCCACGT
GGAACGCACGAGCGTGTGGATCGGCACCGGCTCGGAACCGGTGCGGCTGGGCCAGATTCAGCCGCCCGGCAAGAAACTCA
TGAACGCGGCCGACTGGGCGCGGGGCGCACGGCTGGACCTGGCCGCACGGGCAACATGA

Upstream 100 bases:

>100_bases
CCGGATACAGTATGCATCAGAAATAGTGTATTCCTCTAACTATCGCGCGTGTCGGAATTGTGGCCCACGCCACGTCGGCG
GCGCTTCTTAGACTGGGCGC

Downstream 100 bases:

>100_bases
CCCCTAGATCGCGTGGGCCGCGCCGCCGGCCGCTGGACCCGGCGCGTCGTGCGGCCTTCGAGACGCTGCGGGCGGTTAGT
GCGCGCGACGCCTACGCGAA

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MRLVFAGTPEPALASLRRLIESPSHDVIAVLTRPDAASGRRGKPQPSPVAREAAERGIPVLRPSRPNSAEFVAELSDLAP
ECCAVVAYGALLGGPLLAVPPHGWVNLHFSLLPAWRGAAPVQAAIAAGDTITGATTFQIEPSLDSGPIYGVVTEVIQPTD
TAGDLLKRLAVSGAALLSTTLDGIADQRLTPRPQPADGVSVAPKITVANARVRWDLPAAVVERRIRAVTPNPGAWTLIGD
LRVKLGPVHLDAAHRPSKPLPPGGIHVERTSVWIGTGSEPVRLGQIQPPGKKLMNAADWARGARLDLAARAT

Sequences:

>Translated_312_residues
MRLVFAGTPEPALASLRRLIESPSHDVIAVLTRPDAASGRRGKPQPSPVAREAAERGIPVLRPSRPNSAEFVAELSDLAP
ECCAVVAYGALLGGPLLAVPPHGWVNLHFSLLPAWRGAAPVQAAIAAGDTITGATTFQIEPSLDSGPIYGVVTEVIQPTD
TAGDLLKRLAVSGAALLSTTLDGIADQRLTPRPQPADGVSVAPKITVANARVRWDLPAAVVERRIRAVTPNPGAWTLIGD
LRVKLGPVHLDAAHRPSKPLPPGGIHVERTSVWIGTGSEPVRLGQIQPPGKKLMNAADWARGARLDLAARAT
>Mature_312_residues
MRLVFAGTPEPALASLRRLIESPSHDVIAVLTRPDAASGRRGKPQPSPVAREAAERGIPVLRPSRPNSAEFVAELSDLAP
ECCAVVAYGALLGGPLLAVPPHGWVNLHFSLLPAWRGAAPVQAAIAAGDTITGATTFQIEPSLDSGPIYGVVTEVIQPTD
TAGDLLKRLAVSGAALLSTTLDGIADQRLTPRPQPADGVSVAPKITVANARVRWDLPAAVVERRIRAVTPNPGAWTLIGD
LRVKLGPVHLDAAHRPSKPLPPGGIHVERTSVWIGTGSEPVRLGQIQPPGKKLMNAADWARGARLDLAARAT

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family

Homologues:

Organism=Homo sapiens, GI164663775, Length=215, Percent_Identity=30.6976744186047, Blast_Score=99, Evalue=6e-21,
Organism=Homo sapiens, GI21614513, Length=304, Percent_Identity=24.6710526315789, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI238814322, Length=258, Percent_Identity=27.5193798449612, Blast_Score=83, Evalue=3e-16,
Organism=Escherichia coli, GI1789683, Length=300, Percent_Identity=38.3333333333333, Blast_Score=175, Evalue=3e-45,
Organism=Escherichia coli, GI1788589, Length=289, Percent_Identity=30.1038062283737, Blast_Score=104, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI133930964, Length=309, Percent_Identity=25.8899676375405, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI45550868, Length=311, Percent_Identity=31.5112540192926, Blast_Score=101, Evalue=7e-22,
Organism=Drosophila melanogaster, GI28571984, Length=226, Percent_Identity=34.5132743362832, Blast_Score=100, Evalue=1e-21,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): FMT_MYCBO (P64135)

Other databases:

- EMBL:   BX248338
- RefSeq:   NP_855093.1
- ProteinModelPortal:   P64135
- SMR:   P64135
- EnsemblBacteria:   EBMYCT00000016671
- GeneID:   1092298
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb1441
- GeneTree:   EBGT00050000015792
- HOGENOM:   HBG571560
- OMA:   IMQMDEG
- ProtClustDB:   PRK00005
- BioCyc:   MBOV233413:MB1441-MONOMER
- BRENDA:   2.1.2.9
- HAMAP:   MF_00182
- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR015518
- Gene3D:   G3DSA:3.10.25.10
- Gene3D:   G3DSA:3.40.50.170
- PANTHER:   PTHR11138
- TIGRFAMs:   TIGR00460

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N; SSF50486 FMT_C_like; SSF53328 formyl_transf

EC number: =2.1.2.9

Molecular weight: Translated: 32692; Mature: 32692

Theoretical pI: Translated: 10.30; Mature: 10.30

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLVFAGTPEPALASLRRLIESPSHDVIAVLTRPDAASGRRGKPQPSPVAREAAERGIPV
CEEEECCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCE
LRPSRPNSAEFVAELSDLAPECCAVVAYGALLGGPLLAVPPHGWVNLHFSLLPAWRGAAP
ECCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHCCCEEEECCCCEEEEEEEEEECCCCCCC
VQAAIAAGDTITGATTFQIEPSLDSGPIYGVVTEVIQPTDTAGDLLKRLAVSGAALLSTT
HHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHH
LDGIADQRLTPRPQPADGVSVAPKITVANARVRWDLPAAVVERRIRAVTPNPGAWTLIGD
HHHHHCCCCCCCCCCCCCCCCCCEEEEECCEEEECCCHHHHHHHHEECCCCCCCEEEEEE
LRVKLGPVHLDAAHRPSKPLPPGGIHVERTSVWIGTGSEPVRLGQIQPPGKKLMNAADWA
EEEEEEEEEECCCCCCCCCCCCCCEEEEEEEEEEECCCCCEEECCCCCCHHHHHHHHHHC
RGARLDLAARAT
CCCEEEEEECCC
>Mature Secondary Structure
MRLVFAGTPEPALASLRRLIESPSHDVIAVLTRPDAASGRRGKPQPSPVAREAAERGIPV
CEEEECCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCE
LRPSRPNSAEFVAELSDLAPECCAVVAYGALLGGPLLAVPPHGWVNLHFSLLPAWRGAAP
ECCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHCCCEEEECCCCEEEEEEEEEECCCCCCC
VQAAIAAGDTITGATTFQIEPSLDSGPIYGVVTEVIQPTDTAGDLLKRLAVSGAALLSTT
HHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHH
LDGIADQRLTPRPQPADGVSVAPKITVANARVRWDLPAAVVERRIRAVTPNPGAWTLIGD
HHHHHCCCCCCCCCCCCCCCCCCEEEEECCEEEECCCHHHHHHHHEECCCCCCCEEEEEE
LRVKLGPVHLDAAHRPSKPLPPGGIHVERTSVWIGTGSEPVRLGQIQPPGKKLMNAADWA
EEEEEEEEEECCCCCCCCCCCCCCEEEEEEEEEEECCCCCEEECCCCCCHHHHHHHHHHC
RGARLDLAARAT
CCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972