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The map label for this gene is pitA

Identifier: 121636463

GI number: 121636463

Start: 667102

End: 668355

Strand: Reverse

Name: pitA

Synonym: BCG_0589c

Alternate gene names: 121636463

Gene position: 668355-667102 (Counterclockwise)

Preceding gene: 121636464

Following gene: 121636462

Centisome position: 15.28

GC content: 67.15

Gene sequence:

>1254_bases
GTGAACCTTCAGTTGTTCCTTTTGCTCATTGTCGTCGTGACGGCATTGGCGTTCGACTTCACCAACGGGTTCCACGACAC
CGGAAACGCCATGGCGACCTCGATTGCCAGCGGCGCCCTGGCACCGCGGGTAGCGGTAGCACTTTCTGCCGTGCTGAACC
TGATCGGTGCGTTTTTGTCCACCGCCGTGGCGGCCACAATCGCCAAGGGTCTGATCGACGCGAATCTGGTGACGCTGGAG
TTGGTGTTCGCCGGCCTGGTCGGCGGGATCGTCTGGAACCTGTTGACCTGGTTGCTGGGCATTCCGTCGAGTTCCTCACA
TGCGCTGATCGGCGGCATCGTCGGCGCCACAATTGCCGCCGTCGGCCTGCGTGGGGTGATCTGGAGCGGAGTGGTGTCCA
AGGTGATCGTGCCGGCCGTGGTAGCCGCGCTGCTGGCCACGCTGGTCGGAGCAGTCGGCACCTGGCTGGTCTACCGGACG
ACGCGCGGGGTTGCCGAAAAGCGTACGGAACGCGGTTTCCGGCGCGGCCAGATCGGCTCGGCGTCGCTGGTCTCGCTGGC
GCACGGCACCAACGACGCGCAGAAGACGATGGGCGTGATCTTCCTGGCGTTGATGTCCTACGGCGCGGTCAGCACGACGG
CATCGGTGCCGCCGCTGTGGGTGATCGTGAGTTGCGCCGTGGCCATGGCCGCCGGTACCTACCTGGGTGGCTGGCGCATC
ATCCGCACCCTAGGCAAAGGGCTGGTCGAGATCAAACCACCGCAGGGTATGGCCGCCGAGTCGTCATCGGCCGCCGTCAT
TCTGTTGTCCGCGCACTTCGGCTATGCGCTGTCCACAACGCAGGTCGCGACCGGGTCCGTGCTGGGCAGCGGCGTCGGCA
AGCCCGGCGCCGAGGTGCGCTGGGGGGTAGCCGGCCGCATGGTGGTCGCGTGGCTGGTGACGCTCCCGTTGGCCGGGCTG
GTCGGGGCCTTCACCTACGGGCTGGTGCATTTCATCGGTGGCTACCCCGGTGCGATCCTCGGTTTCGCACTGTTGTGGCT
GACCGCCACCGCCATCTGGCTGCGGTCGCGCAGGGCGCCGATCGACCACACCAACGTCAACGCCGACTGGGAAGGCAACC
TGACGGCCGGCCTGGAAGCGGGTGCGCAGCCGCTTGCGGATCAGAGGCCGCCGGTGCCTGCACCGCCGGCTCCGACTCCC
CCACCGAACCACCGAGCACCACAGTTCGGCGTCACCACGAGGAACGCCCCGTGA

Upstream 100 bases:

>100_bases
TACGTGATTGGTAACATCTGACGACGAGTCGAGATACACCCGCATTTCGCGTACTCGTTTGATCGACAACGCAATATCCC
GACCAGCAGAATCAGGCCCT

Downstream 100 bases:

>100_bases
GTGCCTGGTTCAACTACACGGCCACCCTGAAGATCCTGATCTTCAGTCTGCTAGCCGGCGCGCTGCTACCGGGCCTGTTC
GCGGTGGGCGTGCGGCTGCA

Product: putative low-affinity inorganic phosphate transporter integral membrane protein pitA

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 417; Mature: 417

Protein sequence:

>417_residues
MNLQLFLLLIVVVTALAFDFTNGFHDTGNAMATSIASGALAPRVAVALSAVLNLIGAFLSTAVAATIAKGLIDANLVTLE
LVFAGLVGGIVWNLLTWLLGIPSSSSHALIGGIVGATIAAVGLRGVIWSGVVSKVIVPAVVAALLATLVGAVGTWLVYRT
TRGVAEKRTERGFRRGQIGSASLVSLAHGTNDAQKTMGVIFLALMSYGAVSTTASVPPLWVIVSCAVAMAAGTYLGGWRI
IRTLGKGLVEIKPPQGMAAESSSAAVILLSAHFGYALSTTQVATGSVLGSGVGKPGAEVRWGVAGRMVVAWLVTLPLAGL
VGAFTYGLVHFIGGYPGAILGFALLWLTATAIWLRSRRAPIDHTNVNADWEGNLTAGLEAGAQPLADQRPPVPAPPAPTP
PPNHRAPQFGVTTRNAP

Sequences:

>Translated_417_residues
MNLQLFLLLIVVVTALAFDFTNGFHDTGNAMATSIASGALAPRVAVALSAVLNLIGAFLSTAVAATIAKGLIDANLVTLE
LVFAGLVGGIVWNLLTWLLGIPSSSSHALIGGIVGATIAAVGLRGVIWSGVVSKVIVPAVVAALLATLVGAVGTWLVYRT
TRGVAEKRTERGFRRGQIGSASLVSLAHGTNDAQKTMGVIFLALMSYGAVSTTASVPPLWVIVSCAVAMAAGTYLGGWRI
IRTLGKGLVEIKPPQGMAAESSSAAVILLSAHFGYALSTTQVATGSVLGSGVGKPGAEVRWGVAGRMVVAWLVTLPLAGL
VGAFTYGLVHFIGGYPGAILGFALLWLTATAIWLRSRRAPIDHTNVNADWEGNLTAGLEAGAQPLADQRPPVPAPPAPTP
PPNHRAPQFGVTTRNAP
>Mature_417_residues
MNLQLFLLLIVVVTALAFDFTNGFHDTGNAMATSIASGALAPRVAVALSAVLNLIGAFLSTAVAATIAKGLIDANLVTLE
LVFAGLVGGIVWNLLTWLLGIPSSSSHALIGGIVGATIAAVGLRGVIWSGVVSKVIVPAVVAALLATLVGAVGTWLVYRT
TRGVAEKRTERGFRRGQIGSASLVSLAHGTNDAQKTMGVIFLALMSYGAVSTTASVPPLWVIVSCAVAMAAGTYLGGWRI
IRTLGKGLVEIKPPQGMAAESSSAAVILLSAHFGYALSTTQVATGSVLGSGVGKPGAEVRWGVAGRMVVAWLVTLPLAGL
VGAFTYGLVHFIGGYPGAILGFALLWLTATAIWLRSRRAPIDHTNVNADWEGNLTAGLEAGAQPLADQRPPVPAPPAPTP
PPNHRAPQFGVTTRNAP

Specific function: Low-affinity inorganic phosphate transport

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily

Homologues:

Organism=Homo sapiens, GI31543630, Length=161, Percent_Identity=29.1925465838509, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI5803173, Length=167, Percent_Identity=31.7365269461078, Blast_Score=82, Evalue=1e-15,
Organism=Escherichia coli, GI1789907, Length=222, Percent_Identity=32.4324324324324, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI1789360, Length=223, Percent_Identity=29.5964125560538, Blast_Score=89, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17539280, Length=174, Percent_Identity=28.735632183908, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI71981576, Length=171, Percent_Identity=30.9941520467836, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI32566716, Length=178, Percent_Identity=30.3370786516854, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI25146401, Length=172, Percent_Identity=27.906976744186, Blast_Score=76, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6319773, Length=166, Percent_Identity=28.3132530120482, Blast_Score=73, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PIT_MYCBO (P59950)

Other databases:

- EMBL:   BX248335
- RefSeq:   NP_854220.1
- EnsemblBacteria:   EBMYCT00000016306
- GeneID:   1091637
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb0559c
- GeneTree:   EBGT00050000017317
- HOGENOM:   HBG681698
- OMA:   GDAQKTM
- ProtClustDB:   CLSK871824
- BioCyc:   MBOV233413:MB0559C-MONOMER
- InterPro:   IPR001204
- PANTHER:   PTHR11101

Pfam domain/function: PF01384 PHO4

EC number: NA

Molecular weight: Translated: 42735; Mature: 42735

Theoretical pI: Translated: 10.68; Mature: 10.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x134f0f7c)-; HASH(0x139f2eb4)-; HASH(0x142c89b8)-; HASH(0x1404a2c8)-; HASH(0x141d1674)-; HASH(0x130a8e3c)-; HASH(0x14156f08)-; HASH(0x141cc408)-;

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCH
>Mature Secondary Structure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CCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12788972