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The map label for this gene is menE

Identifier: 121636460

GI number: 121636460

Start: 665308

End: 666396

Strand: Reverse

Name: menE

Synonym: BCG_0586c

Alternate gene names: 121636460

Gene position: 666396-665308 (Counterclockwise)

Preceding gene: 121636461

Following gene: 121636459

Centisome position: 15.23

GC content: 69.05

Gene sequence:

>1089_bases
GTGCTGGGTGGCAGCGACCCGGCATTGGTCGCGGTGCCCACCCAGCATGAGTCCTTGCTGGGCGCTTTGCGAGTGGGCGA
GCAGATTGACGACGACGTCGCCCTGGTAGTGACGACGTCAGGAACCACGGGACCGCCCAAGGGCGCCATGTTGACCGCGG
CGGCCTTGACCGCCAGCGCCTCGGCCGCCCACGACCGGCTCGGCGGACCGGGCAGCTGGCTGTTGGCTGTGCCGCCGTAT
CACATCGCCGGGCTGGCGGTGCTGGTGCGCAGCGTGATCGCCGGATCAGTTCCTGTCGAACTGAACGTCTCCGCGGGATT
CGATGTCACCGAATTACCCAACGCGATAAAGAGATTGGGTTCTGGCCGGCGATACACGTCGCTGGTCGCCGCACAGTTGG
CCAAGGCACTTACCGACCCGGCGGCCACGGCCGCGCTGGCCGAATTGGACGCGGTGCTGATCGGCGGCGGGCCGGCCCCC
CGGCCCATCCTGGACGCCGCGGCCGCCGCCGGCATCACGGTGGTGCGCACCTACGGCATGAGCGAGACCTCGGGCGGCTG
TGTCTACGACGGCGTTCCGCTCGACGGGGTCCGGCTGAGGGTGCTGGCCGGCGGCCGCATAGCTATCGGCGGTGCGACCC
TGGCCAAGGGCTATCGCAACCCGGTCTCGCCCGATCCGTTCGCCGAGCCAGGCTGGTTTCACACCGACGACCTTGGCGCC
CTTGAATCGGGTGATTCGGGTGTGCTGACCGTGCTGGGCCGAGCCGACGAAGCGATCAGCACGGGCGGATTCACCGTGCT
GCCGCAGCCAGTGGAGGCCGCACTGGGCACCCACCCTGCGGTGCGTGACTGCGCGGTTTTTGGACTTGCCGACGACCGAC
TCGGTCAGCGAGTGGTCGCCGCGATTGTGGTCGGCGACGGATGCCCACCACCAACGCTAGAAGCGCTGCGGGCGCATGTC
GCGCGCACCCTGGACGTCACCGCCGCGCCCCGAGAGCTACATGTCGTGAACGTGCTACCGCGACGCGGCATCGGCAAGGT
GGACCGGGCAGCGTTGGTGCGCCGGTTCGCCGGTGAAGCCGATCAATAG

Upstream 100 bases:

>100_bases
ATGACGTCCGCGACCGCGAGGAGCACGCATAGCCATCCTGCGTGCGCTGCACGTCCCGGCCGGTTCTGCTACCGCGCTGC
TGCTGCCTGCCCTGCAACGA

Downstream 100 bases:

>100_bases
GCTGATCGGTCGTGAGGATCGGTCGCCGCGAGGGATTGGCCGTCGCGATCGGGTTTGTCCTGGTCGGCGCCGCGTTCGTA
CTGCCGCGGTTGAACCTGGG

Product: O-succinylbenzoic acid--CoA ligase

Products: NA

Alternate protein names: OSB-CoA synthetase; o-succinylbenzoyl-CoA synthetase

Number of amino acids: Translated: 362; Mature: 362

Protein sequence:

>362_residues
MLGGSDPALVAVPTQHESLLGALRVGEQIDDDVALVVTTSGTTGPPKGAMLTAAALTASASAAHDRLGGPGSWLLAVPPY
HIAGLAVLVRSVIAGSVPVELNVSAGFDVTELPNAIKRLGSGRRYTSLVAAQLAKALTDPAATAALAELDAVLIGGGPAP
RPILDAAAAAGITVVRTYGMSETSGGCVYDGVPLDGVRLRVLAGGRIAIGGATLAKGYRNPVSPDPFAEPGWFHTDDLGA
LESGDSGVLTVLGRADEAISTGGFTVLPQPVEAALGTHPAVRDCAVFGLADDRLGQRVVAAIVVGDGCPPPTLEALRAHV
ARTLDVTAAPRELHVVNVLPRRGIGKVDRAALVRRFAGEADQ

Sequences:

>Translated_362_residues
MLGGSDPALVAVPTQHESLLGALRVGEQIDDDVALVVTTSGTTGPPKGAMLTAAALTASASAAHDRLGGPGSWLLAVPPY
HIAGLAVLVRSVIAGSVPVELNVSAGFDVTELPNAIKRLGSGRRYTSLVAAQLAKALTDPAATAALAELDAVLIGGGPAP
RPILDAAAAAGITVVRTYGMSETSGGCVYDGVPLDGVRLRVLAGGRIAIGGATLAKGYRNPVSPDPFAEPGWFHTDDLGA
LESGDSGVLTVLGRADEAISTGGFTVLPQPVEAALGTHPAVRDCAVFGLADDRLGQRVVAAIVVGDGCPPPTLEALRAHV
ARTLDVTAAPRELHVVNVLPRRGIGKVDRAALVRRFAGEADQ
>Mature_362_residues
MLGGSDPALVAVPTQHESLLGALRVGEQIDDDVALVVTTSGTTGPPKGAMLTAAALTASASAAHDRLGGPGSWLLAVPPY
HIAGLAVLVRSVIAGSVPVELNVSAGFDVTELPNAIKRLGSGRRYTSLVAAQLAKALTDPAATAALAELDAVLIGGGPAP
RPILDAAAAAGITVVRTYGMSETSGGCVYDGVPLDGVRLRVLAGGRIAIGGATLAKGYRNPVSPDPFAEPGWFHTDDLGA
LESGDSGVLTVLGRADEAISTGGFTVLPQPVEAALGTHPAVRDCAVFGLADDRLGQRVVAAIVVGDGCPPPTLEALRAHV
ARTLDVTAAPRELHVVNVLPRRGIGKVDRAALVRRFAGEADQ

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA). May be involved in the biosynthesis of menaquinone

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily

Homologues:

Organism=Homo sapiens, GI187761345, Length=388, Percent_Identity=27.0618556701031, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI187761343, Length=388, Percent_Identity=27.0618556701031, Blast_Score=95, Evalue=1e-19,
Organism=Escherichia coli, GI1788595, Length=275, Percent_Identity=30.1818181818182, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI145693145, Length=357, Percent_Identity=26.6106442577031, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788107, Length=369, Percent_Identity=25.7452574525745, Blast_Score=85, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17558820, Length=346, Percent_Identity=28.6127167630058, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI32563687, Length=359, Percent_Identity=27.8551532033426, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17557194, Length=378, Percent_Identity=26.4550264550265, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17538037, Length=367, Percent_Identity=26.158038147139, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17531443, Length=362, Percent_Identity=24.585635359116, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17556356, Length=222, Percent_Identity=26.5765765765766, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6319699, Length=357, Percent_Identity=24.3697478991597, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6319264, Length=154, Percent_Identity=33.7662337662338, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24581924, Length=405, Percent_Identity=24.4444444444444, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI21355181, Length=360, Percent_Identity=24.7222222222222, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24653035, Length=366, Percent_Identity=24.8633879781421, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MENE_MYCTU (O06408)

Other databases:

- EMBL:   BX842573
- EMBL:   AE000516
- PIR:   A70547
- RefSeq:   NP_215056.1
- RefSeq:   NP_334975.1
- ProteinModelPortal:   O06408
- SMR:   O06408
- EnsemblBacteria:   EBMYCT00000002933
- EnsemblBacteria:   EBMYCT00000069693
- GeneID:   887507
- GeneID:   924889
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT0567
- KEGG:   mtu:Rv0542c
- TIGR:   MT0567
- TubercuList:   Rv0542c
- GeneTree:   EBGT00070000031754
- HOGENOM:   HBG547964
- OMA:   HPAFAEP
- ProtClustDB:   PRK07824
- GO:   GO:0040007
- InterPro:   IPR020845
- InterPro:   IPR000873

Pfam domain/function: PF00501 AMP-binding

EC number: =6.2.1.26

Molecular weight: Translated: 36619; Mature: 36619

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGGSDPALVAVPTQHESLLGALRVGEQIDDDVALVVTTSGTTGPPKGAMLTAAALTASA
CCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCEEEHHHHHHCC
SAAHDRLGGPGSWLLAVPPYHIAGLAVLVRSVIAGSVPVELNVSAGFDVTELPNAIKRLG
HHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHC
SGRRYTSLVAAQLAKALTDPAATAALAELDAVLIGGGPAPRPILDAAAAAGITVVRTYGM
CCCHHHHHHHHHHHHHHCCHHHHHHHHHHCEEEECCCCCCCHHHHHHHHCCEEEEEEECC
SETSGGCVYDGVPLDGVRLRVLAGGRIAIGGATLAKGYRNPVSPDPFAEPGWFHTDDLGA
CCCCCCEEECCCCCCCEEEEEEECCEEEECCHHHHHCCCCCCCCCCCCCCCCEECCCCCC
LESGDSGVLTVLGRADEAISTGGFTVLPQPVEAALGTHPAVRDCAVFGLADDRLGQRVVA
CCCCCCCEEEEEECCCCHHCCCCEEECCCHHHHHHCCCCCHHHHEEEECCHHHHHHEEEE
AIVVGDGCPPPTLEALRAHVARTLDVTAAPRELHVVNVLPRRGIGKVDRAALVRRFAGEA
EEEECCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCC
DQ
CC
>Mature Secondary Structure
MLGGSDPALVAVPTQHESLLGALRVGEQIDDDVALVVTTSGTTGPPKGAMLTAAALTASA
CCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCEEEHHHHHHCC
SAAHDRLGGPGSWLLAVPPYHIAGLAVLVRSVIAGSVPVELNVSAGFDVTELPNAIKRLG
HHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHC
SGRRYTSLVAAQLAKALTDPAATAALAELDAVLIGGGPAPRPILDAAAAAGITVVRTYGM
CCCHHHHHHHHHHHHHHCCHHHHHHHHHHCEEEECCCCCCCHHHHHHHHCCEEEEEEECC
SETSGGCVYDGVPLDGVRLRVLAGGRIAIGGATLAKGYRNPVSPDPFAEPGWFHTDDLGA
CCCCCCEEECCCCCCCEEEEEEECCEEEECCHHHHHCCCCCCCCCCCCCCCCEECCCCCC
LESGDSGVLTVLGRADEAISTGGFTVLPQPVEAALGTHPAVRDCAVFGLADDRLGQRVVA
CCCCCCCEEEEEECCCCHHCCCCEEECCCHHHHHHCCCCCHHHHEEEECCHHHHHHEEEE
AIVVGDGCPPPTLEALRAHVARTLDVTAAPRELHVVNVLPRRGIGKVDRAALVRRFAGEA
EEEECCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCC
DQ
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036