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The map label for this gene is trmH [H]

Identifier: 121636293

GI number: 121636293

Start: 487081

End: 487632

Strand: Reverse

Name: trmH [H]

Synonym: BCG_0418c

Alternate gene names: 121636293

Gene position: 487632-487081 (Counterclockwise)

Preceding gene: 121636294

Following gene: 121636289

Centisome position: 11.15

GC content: 68.48

Gene sequence:

>552_bases
GTGTTGTTGCGCGACGGCGATGCTCGCAACGTCGTCGACGCCTACCGGTACTGGACCCGAGAGGCGATCATCGCCGACAT
CGATACGCGCCGTCACCCCTTGCACGTGGCGATCGAGAACTTCGGACACGATGCCAATATCGGCTCGGTGGTGCGCACCG
CCAATGCATTCGCCGTGCACACCGTGCACATCGTCGGGCGTCGGCGGTGGAATCGGCGCGGCGCCATGGTGACCGACCGC
TATCAGCGGTTATGCCACCACGACAGCACCACCGGGCTGCTGGAGTTCGCGGCGGGCGCCGGCTTGACCGTGGTGGCGGT
GGACAACGTCCCGGGTGCGGCGCGCCTGGAGCAGACCGCGTTGCCGCGGGAATGCCTGCTGTTATTCGGCCAGGAAGGGC
CCGGCATTACCGACGACGCCCGTGCCGGCGCGGCGGTCACGGTGTCGATCGCCCAGTTCGGGTCGACGCGAAGCATTAAC
GCCGGCGTGGCCGCCGGAATCGCGATGCACGCCTGGATACGGCAGCACGCGGACCTGGGCCGCGCCTGGTAG

Upstream 100 bases:

>100_bases
GATGAGTGCGCTTGGGCCCGGGCCTACGGAATGGGGCGCGCCGACCGGCGGCGTGGGGCCCTGGGCAGGCGACCTCCCGG
ACGACCCCCGATACGACCCG

Downstream 100 bases:

>100_bases
GCCTCGGCGAGCAGACACAAAATCGCACGAATCAACCGCAAACCGTGCGATTTTGCGTCTGCTCGCCGGCCCGTGCTAGC
GCGGTTGCGCCGGCCTCATC

Product: putative rna methyltransferase

Products: NA

Alternate protein names: tRNA [Gm18] methyltransferase [H]

Number of amino acids: Translated: 183; Mature: 183

Protein sequence:

>183_residues
MLLRDGDARNVVDAYRYWTREAIIADIDTRRHPLHVAIENFGHDANIGSVVRTANAFAVHTVHIVGRRRWNRRGAMVTDR
YQRLCHHDSTTGLLEFAAGAGLTVVAVDNVPGAARLEQTALPRECLLLFGQEGPGITDDARAGAAVTVSIAQFGSTRSIN
AGVAAGIAMHAWIRQHADLGRAW

Sequences:

>Translated_183_residues
MLLRDGDARNVVDAYRYWTREAIIADIDTRRHPLHVAIENFGHDANIGSVVRTANAFAVHTVHIVGRRRWNRRGAMVTDR
YQRLCHHDSTTGLLEFAAGAGLTVVAVDNVPGAARLEQTALPRECLLLFGQEGPGITDDARAGAAVTVSIAQFGSTRSIN
AGVAAGIAMHAWIRQHADLGRAW
>Mature_183_residues
MLLRDGDARNVVDAYRYWTREAIIADIDTRRHPLHVAIENFGHDANIGSVVRTANAFAVHTVHIVGRRRWNRRGAMVTDR
YQRLCHHDSTTGLLEFAAGAGLTVVAVDNVPGAARLEQTALPRECLLLFGQEGPGITDDARAGAAVTVSIAQFGSTRSIN
AGVAAGIAMHAWIRQHADLGRAW

Specific function: Specifically methylates guanosine-18 in various tRNAs [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790083, Length=166, Percent_Identity=28.9156626506024, Blast_Score=65, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022724
- InterPro:   IPR001537 [H]

Pfam domain/function: PF12105 SpoU_methylas_C; PF00588 SpoU_methylase [H]

EC number: =2.1.1.34 [H]

Molecular weight: Translated: 19912; Mature: 19912

Theoretical pI: Translated: 8.78; Mature: 8.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLRDGDARNVVDAYRYWTREAIIADIDTRRHPLHVAIENFGHDANIGSVVRTANAFAVH
CCCCCCCCCHHHHHHHHHHHHHHEEECCCCCCCEEEEEECCCCCCCHHHHHHHCCCCEEE
TVHIVGRRRWNRRGAMVTDRYQRLCHHDSTTGLLEFAAGAGLTVVAVDNVPGAARLEQTA
EEEEEHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHHHCCCCEEEEEECCCCCHHHHHHHC
LPRECLLLFGQEGPGITDDARAGAAVTVSIAQFGSTRSINAGVAAGIAMHAWIRQHADLG
CCHHHHEEECCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCC
RAW
CCC
>Mature Secondary Structure
MLLRDGDARNVVDAYRYWTREAIIADIDTRRHPLHVAIENFGHDANIGSVVRTANAFAVH
CCCCCCCCCHHHHHHHHHHHHHHEEECCCCCCCEEEEEECCCCCCCHHHHHHHCCCCEEE
TVHIVGRRRWNRRGAMVTDRYQRLCHHDSTTGLLEFAAGAGLTVVAVDNVPGAARLEQTA
EEEEEHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHHHCCCCEEEEEECCCCCHHHHHHHC
LPRECLLLFGQEGPGITDDARAGAAVTVSIAQFGSTRSINAGVAAGIAMHAWIRQHADLG
CCHHHHEEECCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCC
RAW
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]