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The map label for this gene is fliM [H]

Identifier: 120613024

GI number: 120613024

Start: 4878230

End: 4879234

Strand: Reverse

Name: fliM [H]

Synonym: Aave_4388

Alternate gene names: 120613024

Gene position: 4879234-4878230 (Counterclockwise)

Preceding gene: 120613025

Following gene: 120613023

Centisome position: 91.15

GC content: 63.78

Gene sequence:

>1005_bases
ATGAGCGAGTCATTCCTTTCCCAGGAAGAAGTCGATGCCCTGCTGGAAGGCGTCACCGGCGAGAGCCAGAAGTCCGTGGA
GGAAGCGGCGGACACCGGTGCCATCCGCAACTACGACATCTCCAGCCAGGAGCGCATCGTCCGTGGCCGCATGCCGACGA
TGGAAATCGTCAACGAGCGGTTCGCGCGCAATTTCCGCATCGGCCTGTTCAACTTCATCCGCCGCAGCCCCGAGATCTCG
GTGGGAACAGTGTCCGTGCAGCGCTACAGCGCCTTCCTGCGCGAGCTGGCGGTGCCCACCAACTTCAACATCGTGGCCAT
CCGGCCGCTGCGCGGCAGCGGACTGATCGTCTGCGAGCCGTCCCTCGTGTTCGGCATCATCGACACGCTCTACGGCGGCG
TGGGCAAGTTCCAGACCCGCATCGAGGGCCGGGATTTTTCCCCCACCGAGCAGCGCGTCATCAACCGCCTGGTGGACGTG
ATCTGCGCCGAATACAAGAAGGCCTGGCACGGCATCTACCCGCTGGAGCTGGAATACCAGCGCTCCGAGATGCAGCCGCA
GTTCGCGAATATCGCCACCCCGAGCGAGATCGTGGTGTCCACCGCCTTCCAGCTCGAGATCGGCGATCTGTCGGGGGCCA
TCCACATCTGCATGCCCTACGCGACCCTGGAGCCGATCCGCGACGTGCTCTATTCTTCGACGCAGGGTGATTCGATCGAA
GTCGATCGCCGCTGGGTGCGCGTGCTGACGCGCGAGATCCAGGCCGCGGAAGTGACCCTCGTGGCCGAACTGGCCCGGGC
CGACGCCACCGTCGAGCAACTTCTCGCCATGAAGCCCGGCGATTTCATCGAGCTCGACCGCGAGCCACGCATCCAGGCGT
CGATCGGGGGCGTGCCCATCTTCGAGTGCCAGTACGGCACGCACAATTCCAAGTACGCCATCCGCATCGAGGAGTGCCTG
CGCAACCCCGATCTGAACTGGCTGGGAGAAAAGAATGTCAACTGA

Upstream 100 bases:

>100_bases
GACGGCGAAGAGGCCCCGCGCCCCAGGGTCGACAACAATCCGGTGCGGCAAGTGCTCTTCTCCAGTTTCATCATCCAGTG
ACCCGGCAGGAGATTTCCCC

Downstream 100 bases:

>100_bases
AGGCGACAGCAACGACAACGACGGCAAGAGCGCGGCGGACGATCCGTTCGCCGGCTGGGCCGAAGCGCTGGAGGAGCAGA
AGAGCTCCGACGAGTCGCAG

Product: flagellar motor switch protein FliM

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MSESFLSQEEVDALLEGVTGESQKSVEEAADTGAIRNYDISSQERIVRGRMPTMEIVNERFARNFRIGLFNFIRRSPEIS
VGTVSVQRYSAFLRELAVPTNFNIVAIRPLRGSGLIVCEPSLVFGIIDTLYGGVGKFQTRIEGRDFSPTEQRVINRLVDV
ICAEYKKAWHGIYPLELEYQRSEMQPQFANIATPSEIVVSTAFQLEIGDLSGAIHICMPYATLEPIRDVLYSSTQGDSIE
VDRRWVRVLTREIQAAEVTLVAELARADATVEQLLAMKPGDFIELDREPRIQASIGGVPIFECQYGTHNSKYAIRIEECL
RNPDLNWLGEKNVN

Sequences:

>Translated_334_residues
MSESFLSQEEVDALLEGVTGESQKSVEEAADTGAIRNYDISSQERIVRGRMPTMEIVNERFARNFRIGLFNFIRRSPEIS
VGTVSVQRYSAFLRELAVPTNFNIVAIRPLRGSGLIVCEPSLVFGIIDTLYGGVGKFQTRIEGRDFSPTEQRVINRLVDV
ICAEYKKAWHGIYPLELEYQRSEMQPQFANIATPSEIVVSTAFQLEIGDLSGAIHICMPYATLEPIRDVLYSSTQGDSIE
VDRRWVRVLTREIQAAEVTLVAELARADATVEQLLAMKPGDFIELDREPRIQASIGGVPIFECQYGTHNSKYAIRIEECL
RNPDLNWLGEKNVN
>Mature_333_residues
SESFLSQEEVDALLEGVTGESQKSVEEAADTGAIRNYDISSQERIVRGRMPTMEIVNERFARNFRIGLFNFIRRSPEISV
GTVSVQRYSAFLRELAVPTNFNIVAIRPLRGSGLIVCEPSLVFGIIDTLYGGVGKFQTRIEGRDFSPTEQRVINRLVDVI
CAEYKKAWHGIYPLELEYQRSEMQPQFANIATPSEIVVSTAFQLEIGDLSGAIHICMPYATLEPIRDVLYSSTQGDSIEV
DRRWVRVLTREIQAAEVTLVAELARADATVEQLLAMKPGDFIELDREPRIQASIGGVPIFECQYGTHNSKYAIRIEECLR
NPDLNWLGEKNVN

Specific function: FliM is one of three proteins (FliG, FliN, FliM) that form the rotor-mounted switch complex (C ring), located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins, in addition to contacting components of the mot

COG id: COG1868

COG function: function code N; Flagellar motor switch protein

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein. Bacterial flagellum basal body [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FliM family [H]

Homologues:

Organism=Escherichia coli, GI1788255, Length=335, Percent_Identity=47.7611940298507, Blast_Score=327, Evalue=9e-91,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001689
- InterPro:   IPR001543 [H]

Pfam domain/function: PF02154 FliM; PF01052 SpoA [H]

EC number: NA

Molecular weight: Translated: 37583; Mature: 37452

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSESFLSQEEVDALLEGVTGESQKSVEEAADTGAIRNYDISSQERIVRGRMPTMEIVNER
CCCHHCCHHHHHHHHHCCCCCHHHHHHHHHHCCCEECCCCCHHHHHHHCCCCHHHHHHHH
FARNFRIGLFNFIRRSPEISVGTVSVQRYSAFLRELAVPTNFNIVAIRPLRGSGLIVCEP
HHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEECC
SLVFGIIDTLYGGVGKFQTRIEGRDFSPTEQRVINRLVDVICAEYKKAWHGIYPLELEYQ
HHHHHHHHHHHCCCHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEC
RSEMQPQFANIATPSEIVVSTAFQLEIGDLSGAIHICMPYATLEPIRDVLYSSTQGDSIE
HHHCCCHHHCCCCCHHHHEEEEEEEEECCCCCEEEEEECHHHHHHHHHHHHCCCCCCCCH
VDRRWVRVLTREIQAAEVTLVAELARADATVEQLLAMKPGDFIELDREPRIQASIGGVPI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCEEEECCCCEE
FECQYGTHNSKYAIRIEECLRNPDLNWLGEKNVN
EEEECCCCCCEEEEEHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
SESFLSQEEVDALLEGVTGESQKSVEEAADTGAIRNYDISSQERIVRGRMPTMEIVNER
CCHHCCHHHHHHHHHCCCCCHHHHHHHHHHCCCEECCCCCHHHHHHHCCCCHHHHHHHH
FARNFRIGLFNFIRRSPEISVGTVSVQRYSAFLRELAVPTNFNIVAIRPLRGSGLIVCEP
HHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEECC
SLVFGIIDTLYGGVGKFQTRIEGRDFSPTEQRVINRLVDVICAEYKKAWHGIYPLELEYQ
HHHHHHHHHHHCCCHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEC
RSEMQPQFANIATPSEIVVSTAFQLEIGDLSGAIHICMPYATLEPIRDVLYSSTQGDSIE
HHHCCCHHHCCCCCHHHHEEEEEEEEECCCCCEEEEEECHHHHHHHHHHHHCCCCCCCCH
VDRRWVRVLTREIQAAEVTLVAELARADATVEQLLAMKPGDFIELDREPRIQASIGGVPI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCEEEECCCCEE
FECQYGTHNSKYAIRIEECLRNPDLNWLGEKNVN
EEEECCCCCCEEEEEHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 3519573; 9097040; 9278503; 2651416 [H]