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The map label for this gene is paaF [C]

Identifier: 120612989

GI number: 120612989

Start: 4835534

End: 4836337

Strand: Reverse

Name: paaF [C]

Synonym: Aave_4353

Alternate gene names: 120612989

Gene position: 4836337-4835534 (Counterclockwise)

Preceding gene: 120612990

Following gene: 120612988

Centisome position: 90.35

GC content: 71.89

Gene sequence:

>804_bases
ATGACGACCACCGAATACCGCCATCTGCACGTCGGCCAGGCCGGATCGATCGCGCGCGTCACCCTCGCGCGGCCGGAAGT
GCGCAACGCGTTCAGCGACGAGGTCATTGCCGAGCTGACACGCGCGTTCGAGGCGCTGGGCCGGCAGGAGGACGTGCGCG
CCATCGTGCTCGCCGCCGAAGGCCCGGCTTTTTGCGCGGGCGCGGACCTGAACTGGATGCGCCGCATGGCCGACTATTCG
CGCGCGGAGAACGTCGCGGACGCGGGCCGGCTGGCAACGATGCTGCGCACGCTGAACGATTGCCCGAAGCCGACCATCGC
CCGCATCCAGGGCGATGTGTACGCGGGCGGCGTGGGGCTGGTGGCCGCCTGCGACGTGGCGGTGGCGGTGGATACGGCGG
CGTTCTGCCTGAGCGAGGTGCGGCTGGGGCTGATTCCCGCCACCATCGGGCCCTACGTGGTGCGGGCCATGGGCGAGCGC
GCGGCGCGCCGCTATGTCGTGACCGGCGAGCGCTTCAGCGCGCTGGAAGCGCTGCGCGTGGGACTGGTGCACGAGGTGGT
GGATTTCGAGCAGCTCGACGAGAAGCTCGATGCCGTCCTGCAGTCGATCGCCGCGGCCGGGCCGCAGGCGGTGGCCGCGG
GCAAGCGCCTGGTGCGTGACATCGCGGGGCGGCCCATCGACGATGCGCTCATCGCCTCGACGGTGGAGTCCATCGCGGAC
ATCCGGGCGAGCGGCGAGGGGCGCGAGGGCGTGCAGGCCTTCCTGCAGAAGCGCAAGCCGGCCTGGCTGCCGGCTCGCGA
CTGA

Upstream 100 bases:

>100_bases
GCCGCGCGCAATGCGCCCATAGAGGACACGAAGTTCGGCGTTTTCCGCATGTGAGCGCCCCGGGGCTCGCCCATCCTGAC
AGCACAAGGAGGAATCCACC

Downstream 100 bases:

>100_bases
GGACGCCCCCGCGGGCCCGCCCCCATGGATGCCCTCTGGCTGCACATCGTCCAGTGGCTGCACGCCGCCGGCCTGCACGT
GGATGCGGGCACGGCCCAGG

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 267; Mature: 266

Protein sequence:

>267_residues
MTTTEYRHLHVGQAGSIARVTLARPEVRNAFSDEVIAELTRAFEALGRQEDVRAIVLAAEGPAFCAGADLNWMRRMADYS
RAENVADAGRLATMLRTLNDCPKPTIARIQGDVYAGGVGLVAACDVAVAVDTAAFCLSEVRLGLIPATIGPYVVRAMGER
AARRYVVTGERFSALEALRVGLVHEVVDFEQLDEKLDAVLQSIAAAGPQAVAAGKRLVRDIAGRPIDDALIASTVESIAD
IRASGEGREGVQAFLQKRKPAWLPARD

Sequences:

>Translated_267_residues
MTTTEYRHLHVGQAGSIARVTLARPEVRNAFSDEVIAELTRAFEALGRQEDVRAIVLAAEGPAFCAGADLNWMRRMADYS
RAENVADAGRLATMLRTLNDCPKPTIARIQGDVYAGGVGLVAACDVAVAVDTAAFCLSEVRLGLIPATIGPYVVRAMGER
AARRYVVTGERFSALEALRVGLVHEVVDFEQLDEKLDAVLQSIAAAGPQAVAAGKRLVRDIAGRPIDDALIASTVESIAD
IRASGEGREGVQAFLQKRKPAWLPARD
>Mature_266_residues
TTTEYRHLHVGQAGSIARVTLARPEVRNAFSDEVIAELTRAFEALGRQEDVRAIVLAAEGPAFCAGADLNWMRRMADYSR
AENVADAGRLATMLRTLNDCPKPTIARIQGDVYAGGVGLVAACDVAVAVDTAAFCLSEVRLGLIPATIGPYVVRAMGERA
ARRYVVTGERFSALEALRVGLVHEVVDFEQLDEKLDAVLQSIAAAGPQAVAAGKRLVRDIAGRPIDDALIASTVESIADI
RASGEGREGVQAFLQKRKPAWLPARD

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI31542718, Length=261, Percent_Identity=32.5670498084291, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI194097323, Length=255, Percent_Identity=30.1960784313725, Blast_Score=102, Evalue=3e-22,
Organism=Homo sapiens, GI70995211, Length=232, Percent_Identity=31.4655172413793, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI4502327, Length=269, Percent_Identity=29.368029739777, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI45643119, Length=222, Percent_Identity=26.5765765765766, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI260275230, Length=222, Percent_Identity=26.5765765765766, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI260274832, Length=222, Percent_Identity=26.5765765765766, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI221136756, Length=256, Percent_Identity=23.4375, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI221136753, Length=256, Percent_Identity=23.4375, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI221307494, Length=256, Percent_Identity=23.4375, Blast_Score=71, Evalue=8e-13,
Organism=Homo sapiens, GI37594471, Length=182, Percent_Identity=27.4725274725275, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI37594469, Length=244, Percent_Identity=24.5901639344262, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI62530384, Length=193, Percent_Identity=30.0518134715026, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI4757966, Length=217, Percent_Identity=22.1198156682028, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI51538581, Length=217, Percent_Identity=22.1198156682028, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI25453481, Length=256, Percent_Identity=21.09375, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI51538573, Length=256, Percent_Identity=21.09375, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI68989263, Length=191, Percent_Identity=29.3193717277487, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1787659, Length=248, Percent_Identity=31.8548387096774, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI221142681, Length=267, Percent_Identity=30.3370786516854, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI1788597, Length=249, Percent_Identity=28.9156626506024, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI87082183, Length=269, Percent_Identity=26.3940520446097, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI1787660, Length=250, Percent_Identity=29.2, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788682, Length=184, Percent_Identity=30.4347826086957, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1790281, Length=188, Percent_Identity=29.7872340425532, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI25145438, Length=259, Percent_Identity=30.1158301158301, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17554946, Length=253, Percent_Identity=29.6442687747036, Blast_Score=94, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17536985, Length=232, Percent_Identity=31.4655172413793, Blast_Score=94, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI17540306, Length=255, Percent_Identity=27.4509803921569, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17535521, Length=257, Percent_Identity=24.9027237354086, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17549921, Length=207, Percent_Identity=25.1207729468599, Blast_Score=74, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17534483, Length=248, Percent_Identity=25.4032258064516, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17540714, Length=210, Percent_Identity=27.6190476190476, Blast_Score=74, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI25144160, Length=268, Percent_Identity=27.2388059701493, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI25144157, Length=268, Percent_Identity=27.2388059701493, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17560910, Length=190, Percent_Identity=29.4736842105263, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI19922422, Length=252, Percent_Identity=23.8095238095238, Blast_Score=88, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24653139, Length=255, Percent_Identity=29.0196078431373, Blast_Score=87, Evalue=8e-18,
Organism=Drosophila melanogaster, GI20129971, Length=190, Percent_Identity=31.0526315789474, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24653477, Length=190, Percent_Identity=31.0526315789474, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI19920382, Length=212, Percent_Identity=32.5471698113208, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24654903, Length=221, Percent_Identity=26.2443438914027, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24583165, Length=220, Percent_Identity=27.7272727272727, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 28594; Mature: 28463

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTEYRHLHVGQAGSIARVTLARPEVRNAFSDEVIAELTRAFEALGRQEDVRAIVLAAE
CCCCCCCEEECCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEC
GPAFCAGADLNWMRRMADYSRAENVADAGRLATMLRTLNDCPKPTIARIQGDVYAGGVGL
CCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCEECCHHH
VAACDVAVAVDTAAFCLSEVRLGLIPATIGPYVVRAMGERAARRYVVTGERFSALEALRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHHHH
GLVHEVVDFEQLDEKLDAVLQSIAAAGPQAVAAGKRLVRDIAGRPIDDALIASTVESIAD
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
IRASGEGREGVQAFLQKRKPAWLPARD
HHCCCCCHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TTTEYRHLHVGQAGSIARVTLARPEVRNAFSDEVIAELTRAFEALGRQEDVRAIVLAAE
CCCCCCEEECCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEC
GPAFCAGADLNWMRRMADYSRAENVADAGRLATMLRTLNDCPKPTIARIQGDVYAGGVGL
CCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCEECCHHH
VAACDVAVAVDTAAFCLSEVRLGLIPATIGPYVVRAMGERAARRYVVTGERFSALEALRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHHHH
GLVHEVVDFEQLDEKLDAVLQSIAAAGPQAVAAGKRLVRDIAGRPIDDALIASTVESIAD
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
IRASGEGREGVQAFLQKRKPAWLPARD
HHCCCCCHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA