Click here to switch to the map view.

The map label for this gene is prfC [H]

Identifier: 120612699

GI number: 120612699

Start: 4513297

End: 4514925

Strand: Direct

Name: prfC [H]

Synonym: Aave_4062

Alternate gene names: 120612699

Gene position: 4513297-4514925 (Clockwise)

Preceding gene: 120612698

Following gene: 120612700

Centisome position: 84.32

GC content: 66.85

Gene sequence:

>1629_bases
GTGTCCTACGCTCCCGAAACACGGCGACGCCGCACCTTCGCCATCATCTCCCACCCCGACGCTGGCAAGACCACGCTCAC
CGAAAAGCTGCTGCTGTTCTCGGGCGCGATCCAGATCGCCGGCGCCGTGAAAGGCCGCAAGGCCAGCCGCCACGCCACGT
CGGACTGGATGGAAATCGAGAAGCAGCGCGGCATCTCGGTGGCCTCGTCGGTCATGCAGATGGCCTACCGCGACCACGTG
GTGAACCTGCTGGACACGCCCGGCCACAAGGACTTCTCGGAAGACACCTATCGCGTGCTCACCGCCGTGGATTCCGCGCT
CATGGTGATCGATGCGGCCAACGGCGTGGAATCGCAGACGCGGCGGCTGATCGAGGTCTGCCGGCAGCGCGACACCCCCA
TCATCACTTTCGTCAACAAGATGGACCGCGAGGTGCGCGAGCCCCTGGACATCCTCGACGAGGTCGAGCGCGAGCTCGGC
ATGCCCTGCGTGCCCATGACCTGGCCGGTAGGACAGGGCAAGAGCTTCGGCGGCATCATCAATCTGCGCACCCAGGCGAT
GACCGTGTTCGAGGCCGGCAGCGAGCGCAGGCCGCAGGATTTCGAGGCCATCCCCCTGAGCGAGGCCGACACGCTGCGGG
CCCGGTTCGGACACACCTTCGACGAGGCGCTCGAGAGCATGGAACTGGCCGTGGGCGCCTCCCCGGCGTGGGACCACGAG
GCCTTCCTCGCGGGCAAGCAGACGCCCGTGTTCTTCGGCTCCGGCGTGAACAACTTCGGCGTGATGGAGGTGCTCGACGC
CCTCGTGGATCTCGCGCCCTCGCCGCGCCCGCGCACGAGCAACCTCGTGGTGAACAGGCAGTCCGTCGAGAAGACGGTGT
TGCCCGAGGAAACCAGCTTCTCCGGCGTGGTGTTCAAGGTGCAGGCCAACATGGACGCCAACCACCGCGACCGCATCGCC
TTCGTGCGCGTGGCCTCCGGCAGGTACACCCCGGGCATGAAGCTCAAGGTGCAGCGCACCGCCAAGGAACTGCGTCCCAC
CAGCGTGGTGACTTTCATGAGCCAGCGGCGCGAAGCCGTGGAAGAAGCCTACGCGGGCGACATCATCGGCTTCACGACGC
ATGGCGGCGTGCAGCTGGGCGACACCATCACCGACGGCGCCAGCCTGCAGTTCACGGGCCTGCCCTTCTTCGCGCCCGAA
ATGTTCATGACGGTGGTGCTGAAGAACCCGCTGCGCACCAAACAGCTGCAGCAGGGCCTGGCCCAGCTGGGCGAGGAAGG
CGCCATCCAGGTCTTCAAGCCCGACGCGGGCGGCAACATGCTGCTGGGCGCCGTGGGCCAGCTGCAGTTCGAAGTGGTGC
AGCACCGCCTCAAGACCGAATACGACTGCGACGTGCGGCTGGAAAACTGCCAGTACACGGGGGCGCGCTGGATCACGGCG
GACACGCCGGCGGAGCTGCGGGCCTTCACGGACGCCTACCCGATGCGCATGGCGCACGACGCGGCCGACACCCTGGCCTA
TCTGTGCACCAGCCCCTACGACGTTCGGCTGGCGCAGGAGAGGTTCCCCAAGATCCACTTCCACCCGCTGCGCGAGCATG
CGGGACTTGCCCTGCAAACCAATGCCTGA

Upstream 100 bases:

>100_bases
TAGAATACGCCCCTTTCCACGCCCTGCGGCGGCGCGGCAGCACGCCAACTTGCGCGTGCACTCCGGGCCCCATGCCTGCC
GCACCCTCTCAAGAATCACA

Downstream 100 bases:

>100_bases
GAACTACCCAGCCCGGACGCTCGCCGGCGCACCGGCTCCCAGCCCGGCCTCCGGCGGGCGCCCTTTCGCGGCGATCCTCG
CATTGCTGACGGTGTACTAC

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 542; Mature: 541

Protein sequence:

>542_residues
MSYAPETRRRRTFAIISHPDAGKTTLTEKLLLFSGAIQIAGAVKGRKASRHATSDWMEIEKQRGISVASSVMQMAYRDHV
VNLLDTPGHKDFSEDTYRVLTAVDSALMVIDAANGVESQTRRLIEVCRQRDTPIITFVNKMDREVREPLDILDEVERELG
MPCVPMTWPVGQGKSFGGIINLRTQAMTVFEAGSERRPQDFEAIPLSEADTLRARFGHTFDEALESMELAVGASPAWDHE
AFLAGKQTPVFFGSGVNNFGVMEVLDALVDLAPSPRPRTSNLVVNRQSVEKTVLPEETSFSGVVFKVQANMDANHRDRIA
FVRVASGRYTPGMKLKVQRTAKELRPTSVVTFMSQRREAVEEAYAGDIIGFTTHGGVQLGDTITDGASLQFTGLPFFAPE
MFMTVVLKNPLRTKQLQQGLAQLGEEGAIQVFKPDAGGNMLLGAVGQLQFEVVQHRLKTEYDCDVRLENCQYTGARWITA
DTPAELRAFTDAYPMRMAHDAADTLAYLCTSPYDVRLAQERFPKIHFHPLREHAGLALQTNA

Sequences:

>Translated_542_residues
MSYAPETRRRRTFAIISHPDAGKTTLTEKLLLFSGAIQIAGAVKGRKASRHATSDWMEIEKQRGISVASSVMQMAYRDHV
VNLLDTPGHKDFSEDTYRVLTAVDSALMVIDAANGVESQTRRLIEVCRQRDTPIITFVNKMDREVREPLDILDEVERELG
MPCVPMTWPVGQGKSFGGIINLRTQAMTVFEAGSERRPQDFEAIPLSEADTLRARFGHTFDEALESMELAVGASPAWDHE
AFLAGKQTPVFFGSGVNNFGVMEVLDALVDLAPSPRPRTSNLVVNRQSVEKTVLPEETSFSGVVFKVQANMDANHRDRIA
FVRVASGRYTPGMKLKVQRTAKELRPTSVVTFMSQRREAVEEAYAGDIIGFTTHGGVQLGDTITDGASLQFTGLPFFAPE
MFMTVVLKNPLRTKQLQQGLAQLGEEGAIQVFKPDAGGNMLLGAVGQLQFEVVQHRLKTEYDCDVRLENCQYTGARWITA
DTPAELRAFTDAYPMRMAHDAADTLAYLCTSPYDVRLAQERFPKIHFHPLREHAGLALQTNA
>Mature_541_residues
SYAPETRRRRTFAIISHPDAGKTTLTEKLLLFSGAIQIAGAVKGRKASRHATSDWMEIEKQRGISVASSVMQMAYRDHVV
NLLDTPGHKDFSEDTYRVLTAVDSALMVIDAANGVESQTRRLIEVCRQRDTPIITFVNKMDREVREPLDILDEVERELGM
PCVPMTWPVGQGKSFGGIINLRTQAMTVFEAGSERRPQDFEAIPLSEADTLRARFGHTFDEALESMELAVGASPAWDHEA
FLAGKQTPVFFGSGVNNFGVMEVLDALVDLAPSPRPRTSNLVVNRQSVEKTVLPEETSFSGVVFKVQANMDANHRDRIAF
VRVASGRYTPGMKLKVQRTAKELRPTSVVTFMSQRREAVEEAYAGDIIGFTTHGGVQLGDTITDGASLQFTGLPFFAPEM
FMTVVLKNPLRTKQLQQGLAQLGEEGAIQVFKPDAGGNMLLGAVGQLQFEVVQHRLKTEYDCDVRLENCQYTGARWITAD
TPAELRAFTDAYPMRMAHDAADTLAYLCTSPYDVRLAQERFPKIHFHPLREHAGLALQTNA

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=488, Percent_Identity=28.2786885245902, Blast_Score=188, Evalue=1e-47,
Organism=Homo sapiens, GI19923640, Length=522, Percent_Identity=25.6704980842912, Blast_Score=145, Evalue=1e-34,
Organism=Homo sapiens, GI25306283, Length=409, Percent_Identity=26.4058679706601, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI25306287, Length=320, Percent_Identity=28.125, Blast_Score=126, Evalue=5e-29,
Organism=Homo sapiens, GI157426893, Length=220, Percent_Identity=28.1818181818182, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI94966754, Length=145, Percent_Identity=31.0344827586207, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI4503483, Length=150, Percent_Identity=31.3333333333333, Blast_Score=73, Evalue=7e-13,
Organism=Homo sapiens, GI310132016, Length=117, Percent_Identity=34.1880341880342, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI310110807, Length=117, Percent_Identity=34.1880341880342, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI310123363, Length=117, Percent_Identity=34.1880341880342, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI1790835, Length=532, Percent_Identity=52.4436090225564, Blast_Score=573, Evalue=1e-165,
Organism=Escherichia coli, GI1789738, Length=489, Percent_Identity=27.4028629856851, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI1788922, Length=156, Percent_Identity=33.3333333333333, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI48994988, Length=145, Percent_Identity=36.551724137931, Blast_Score=96, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17533571, Length=493, Percent_Identity=27.789046653144, Blast_Score=171, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17556745, Length=170, Percent_Identity=36.4705882352941, Blast_Score=121, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17557151, Length=132, Percent_Identity=33.3333333333333, Blast_Score=89, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17506493, Length=169, Percent_Identity=29.585798816568, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71988819, Length=133, Percent_Identity=32.3308270676692, Blast_Score=76, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI71988811, Length=133, Percent_Identity=32.3308270676692, Blast_Score=76, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6323098, Length=477, Percent_Identity=28.0922431865828, Blast_Score=177, Evalue=4e-45,
Organism=Saccharomyces cerevisiae, GI6322359, Length=555, Percent_Identity=23.7837837837838, Blast_Score=131, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6323320, Length=158, Percent_Identity=27.2151898734177, Blast_Score=81, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6324707, Length=154, Percent_Identity=31.8181818181818, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6320593, Length=154, Percent_Identity=31.8181818181818, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324166, Length=153, Percent_Identity=31.3725490196078, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24582462, Length=484, Percent_Identity=29.7520661157025, Blast_Score=185, Evalue=6e-47,
Organism=Drosophila melanogaster, GI221458488, Length=510, Percent_Identity=25.8823529411765, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI78706572, Length=157, Percent_Identity=33.1210191082803, Blast_Score=103, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24585711, Length=163, Percent_Identity=31.2883435582822, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24585713, Length=163, Percent_Identity=31.2883435582822, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24585709, Length=163, Percent_Identity=31.2883435582822, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI28574573, Length=176, Percent_Identity=31.8181818181818, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI21357743, Length=142, Percent_Identity=28.169014084507, Blast_Score=65, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 60127; Mature: 59996

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYAPETRRRRTFAIISHPDAGKTTLTEKLLLFSGAIQIAGAVKGRKASRHATSDWMEIE
CCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHEEHHHCCCCCCCCCCHHHHHHH
KQRGISVASSVMQMAYRDHVVNLLDTPGHKDFSEDTYRVLTAVDSALMVIDAANGVESQT
HHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCEEEEEECCCCCHHHH
RRLIEVCRQRDTPIITFVNKMDREVREPLDILDEVERELGMPCVPMTWPVGQGKSFGGII
HHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEE
NLRTQAMTVFEAGSERRPQDFEAIPLSEADTLRARFGHTFDEALESMELAVGASPAWDHE
EEHHHEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCH
AFLAGKQTPVFFGSGVNNFGVMEVLDALVDLAPSPRPRTSNLVVNRQSVEKTVLPEETSF
HHHCCCCCCEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHCCCCCCCC
SGVVFKVQANMDANHRDRIAFVRVASGRYTPGMKLKVQRTAKELRPTSVVTFMSQRREAV
CCEEEEEEECCCCCCCCCEEEEEEECCCCCCCCEEEEHHHHHHCCCHHHHHHHHHHHHHH
EEAYAGDIIGFTTHGGVQLGDTITDGASLQFTGLPFFAPEMFMTVVLKNPLRTKQLQQGL
HHHHCCCEEEEECCCCEEECCEECCCCEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHH
AQLGEEGAIQVFKPDAGGNMLLGAVGQLQFEVVQHRLKTEYDCDVRLENCQYTGARWITA
HHHCCCCCEEEECCCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCEEECCCEECCCEEEEC
DTPAELRAFTDAYPMRMAHDAADTLAYLCTSPYDVRLAQERFPKIHFHPLREHAGLALQT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHCCCCEEEEHHHHCCEEEEE
NA
CC
>Mature Secondary Structure 
SYAPETRRRRTFAIISHPDAGKTTLTEKLLLFSGAIQIAGAVKGRKASRHATSDWMEIE
CCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHEEHHHCCCCCCCCCCHHHHHHH
KQRGISVASSVMQMAYRDHVVNLLDTPGHKDFSEDTYRVLTAVDSALMVIDAANGVESQT
HHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCEEEEEECCCCCHHHH
RRLIEVCRQRDTPIITFVNKMDREVREPLDILDEVERELGMPCVPMTWPVGQGKSFGGII
HHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEE
NLRTQAMTVFEAGSERRPQDFEAIPLSEADTLRARFGHTFDEALESMELAVGASPAWDHE
EEHHHEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCH
AFLAGKQTPVFFGSGVNNFGVMEVLDALVDLAPSPRPRTSNLVVNRQSVEKTVLPEETSF
HHHCCCCCCEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHCCCCCCCC
SGVVFKVQANMDANHRDRIAFVRVASGRYTPGMKLKVQRTAKELRPTSVVTFMSQRREAV
CCEEEEEEECCCCCCCCCEEEEEEECCCCCCCCEEEEHHHHHHCCCHHHHHHHHHHHHHH
EEAYAGDIIGFTTHGGVQLGDTITDGASLQFTGLPFFAPEMFMTVVLKNPLRTKQLQQGL
HHHHCCCEEEEECCCCEEECCEECCCCEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHH
AQLGEEGAIQVFKPDAGGNMLLGAVGQLQFEVVQHRLKTEYDCDVRLENCQYTGARWITA
HHHCCCCCEEEECCCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCEEECCCEECCCEEEEC
DTPAELRAFTDAYPMRMAHDAADTLAYLCTSPYDVRLAQERFPKIHFHPLREHAGLALQT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHCCCCEEEEHHHHCCEEEEE
NA
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA