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The map label for this gene is rsmD [H]

Identifier: 120612511

GI number: 120612511

Start: 4294277

End: 4294906

Strand: Reverse

Name: rsmD [H]

Synonym: Aave_3870

Alternate gene names: 120612511

Gene position: 4294906-4294277 (Counterclockwise)

Preceding gene: 120612512

Following gene: 120612510

Centisome position: 80.24

GC content: 74.13

Gene sequence:

>630_bases
ATGAGCCCGGCGCCGCGCAGGCCTGCCGCGCCCCAGCGCCCTGCGGCGAGTTCAGGGGCCCGGCAGGGCGGGCATGCCGG
TGCGGGCGAAGTGCGCATCATCGGCGGGCTCTGGAAGCGCACGCGGTTGCCGGTGCCGTCGCGCCCGGGGCTGCGGCCCA
CGCCGGACCGGGTCCGGGAAACGCTCTTCAACTGGCTGGGGCAGGACCTGTCCGGATGGCGCTGCGTCGATGCCTTCGCC
GGCACGGGCGCGCTCGGCCTGGAAGCCGCGTCCCGCGGAGCGGTGGAGGTGCTGCTGGTGGAAAGCGACGCGGCCCTCGC
CGATCAACTGCGGCGTTCCTGCGAAAAGCTGGGCGCCGCTGCGGCGCGGGTGCAGCGGGGCGACGGCGTTGCCGTCCTGC
GCCAGCGTGCGCCGGGCTCCGCGCACCTGGTCCTGCTCGATCCGCCTTTCGACGGCGGGCTGTTCGATGCGGCCCTGGCC
GCTGCCGCGCCTGCGCTCGCGGAGGATGGATTCATCTACCTCGAGGCGCCGCAACCCTGGAACGATGCGGCGCTGGCACC
CCTGGGCCTTGCCATCCATCGCCACCTGCGGGCGGGGGCGGTGCATGCCCACCTGATCCGCAAGATTTAG

Upstream 100 bases:

>100_bases
ACCGGGTGGAGGCGCTGACCGCGGAAGACATTCGCGCGGCGATGGCGCGCAAGCTGCAGCCTGCGCGGATGGTGACCGTG
GTGGTCGGCGGGAGCGCGCC

Downstream 100 bases:

>100_bases
CGCCCCCTCGCGCGGCGGGGCGCCATGCCATGGTCCATGGGCCGGATGCGCCTTTTCGTGCACGGGCTGCCCTTGGCGCA
GGAGCGCCGCAATAATCCGC

Product: putative methyltransferase

Products: NA

Alternate protein names: 16S rRNA m2G966 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase [H]

Number of amino acids: Translated: 209; Mature: 208

Protein sequence:

>209_residues
MSPAPRRPAAPQRPAASSGARQGGHAGAGEVRIIGGLWKRTRLPVPSRPGLRPTPDRVRETLFNWLGQDLSGWRCVDAFA
GTGALGLEAASRGAVEVLLVESDAALADQLRRSCEKLGAAAARVQRGDGVAVLRQRAPGSAHLVLLDPPFDGGLFDAALA
AAAPALAEDGFIYLEAPQPWNDAALAPLGLAIHRHLRAGAVHAHLIRKI

Sequences:

>Translated_209_residues
MSPAPRRPAAPQRPAASSGARQGGHAGAGEVRIIGGLWKRTRLPVPSRPGLRPTPDRVRETLFNWLGQDLSGWRCVDAFA
GTGALGLEAASRGAVEVLLVESDAALADQLRRSCEKLGAAAARVQRGDGVAVLRQRAPGSAHLVLLDPPFDGGLFDAALA
AAAPALAEDGFIYLEAPQPWNDAALAPLGLAIHRHLRAGAVHAHLIRKI
>Mature_208_residues
SPAPRRPAAPQRPAASSGARQGGHAGAGEVRIIGGLWKRTRLPVPSRPGLRPTPDRVRETLFNWLGQDLSGWRCVDAFAG
TGALGLEAASRGAVEVLLVESDAALADQLRRSCEKLGAAAARVQRGDGVAVLRQRAPGSAHLVLLDPPFDGGLFDAALAA
AAPALAEDGFIYLEAPQPWNDAALAPLGLAIHRHLRAGAVHAHLIRKI

Specific function: Specifically methylates the guanosine in position 966 of 16S rRNA in the assembled 30S particle [H]

COG id: COG0742

COG function: function code L; N6-adenine-specific methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmD family [H]

Homologues:

Organism=Escherichia coli, GI1789875, Length=191, Percent_Identity=41.8848167539267, Blast_Score=142, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004398 [H]

Pfam domain/function: PF03602 Cons_hypoth95 [H]

EC number: =2.1.1.171 [H]

Molecular weight: Translated: 21872; Mature: 21741

Theoretical pI: Translated: 10.88; Mature: 10.88

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPAPRRPAAPQRPAASSGARQGGHAGAGEVRIIGGLWKRTRLPVPSRPGLRPTPDRVRE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECHHHHHCCCCCCCCCCCCCHHHHHH
TLFNWLGQDLSGWRCVDAFAGTGALGLEAASRGAVEVLLVESDAALADQLRRSCEKLGAA
HHHHHHCCCCCCCEEEHHHCCCCCHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHHHH
AARVQRGDGVAVLRQRAPGSAHLVLLDPPFDGGLFDAALAAAAPALAEDGFIYLEAPQPW
HHHHHCCCCCEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHCCHHHCCCEEEEECCCCC
NDAALAPLGLAIHRHLRAGAVHAHLIRKI
CCCHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SPAPRRPAAPQRPAASSGARQGGHAGAGEVRIIGGLWKRTRLPVPSRPGLRPTPDRVRE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECHHHHHCCCCCCCCCCCCCHHHHHH
TLFNWLGQDLSGWRCVDAFAGTGALGLEAASRGAVEVLLVESDAALADQLRRSCEKLGAA
HHHHHHCCCCCCCEEEHHHCCCCCHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHHHH
AARVQRGDGVAVLRQRAPGSAHLVLLDPPFDGGLFDAALAAAAPALAEDGFIYLEAPQPW
HHHHHCCCCCEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHCCHHHCCCEEEEECCCCC
NDAALAPLGLAIHRHLRAGAVHAHLIRKI
CCCHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]