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The map label for this gene is dnaK [H]

Identifier: 120612382

GI number: 120612382

Start: 4147715

End: 4149547

Strand: Direct

Name: dnaK [H]

Synonym: Aave_3739

Alternate gene names: 120612382

Gene position: 4147715-4149547 (Clockwise)

Preceding gene: 120612381

Following gene: 120612383

Centisome position: 77.49

GC content: 72.61

Gene sequence:

>1833_bases
GTGAGCCAACCGAAATACGCCATCGGCATCGACCTCGGCACCACGCACAGCGCGCTCTCCTGGGTCGATCTGCACACCGA
GGGCGCGGCGCCCGAGATCCTGCCCGTCGCCCAGCAGAGCGGCCCCGGCGCCGTGCAGGACTATTCCCTGCTGCCCTCCT
TCCTCTACGTGCCGCACGACAGCGAATTCGCGCCCGGCGACCTGGCCCTGCCCTGGAACGCCGCGCCCGGCCGCATCACC
GGCGAATGGGCGCGCAGCCACGGCGCGCTCACGCCGATCCGCCTCGTCTCCAGCGCCAAGAGCTGGCTCGGCCATGCCGG
CGTGGACCGCCGCGCCGGCATCCTGCCGGCCGACGCGCCGCCCGAGGTCGAGCGCATCTCGCCGCTGGCCGCCAGCACCC
ACTACCTGCAGCACCTGCGCGACGCCTGGAACCACCGCCACCCGGAGGCGCCGTTCGACCAGCAGTCCGTCACCGTCACC
ATCCCCGCATCGTTCGACCCGGCCGCGCGCGAACTCACGGCCGAGGCCGCCCAGGCCGCCGGCTACCACGGCATCACCCT
GCTGGAAGAGCCCCAGGCCGCGCTCTACAGCTGGATCGCGGGCAGCGCGGGCGACTGGCGCAAGCAGGTGCAGGTGGGCG
ACGTGATCCTCGTCGTCGATGTGGGGGGCGGCACCAGCGACTTCTCGCTCATCGCCGTCACCGAAGACGCCGGCAACCTG
CAGCTCAACCGCGTGGCCGTGGGCGAGCACATCCTGCTCGGCGGCGACAACATGGACCTGGCCCTCGCCCACGGCGTCGC
GCGCGGCCTGGCCGCCGAAGGCAAACAGCTCGACCCCTGGCAGATGCGCGCGCTCACCTACGCCTGCCGCCTGGCCAAGG
AGCAGTTGCTGAGCGACCCCGGCCTGCAGAGCGTGCCCCTGGTCGTGCCCAGCCGCGGCTCCAAGCTCATCGGCGGCTCC
ATCCGCACCGAACTGCAGCGCGAACTGCTCTCCACCATCCTGCTCGAAGGCTTCTTCCCGGCCGTCGAGAGCAGCGCCCG
GCCCATCACCCGCCCGCGCGCCGGCCTCACGCAGATCGGCCTGCCCTACGCGCAGGATGCCGCCGTCACCCGCCACCTGG
CCGCGCTGCTGGGCCGCCAAGTGGGGGCCACCGCGCAGCTGCCGGGCTTCGCCCAGCCCGAGGGCGCGACCTTCCTGCAC
CCCACGGCCGTGCTGTTCAACGGCGGCGTGTTCAAGTCCGAACTGCTGGCCGAACGCGTGCTCTCCACGCTCAACGCCTG
GCTCGCCGCCGACGGCGCGCCCCCCGCCCGCCTGCTGCAGGGCGCCGACATGGACCATGCCGTGGCGCGCGGCGCCGCCT
ACTACGGCCAGGTGCGCCAGGGCCGCGGCATCCGCATCCGCGGTGGCACGGCCCAGGCCTACTACGTCGGCATCGAATCC
AGCATGCCCGCCGTGCCCGGCCTGGAGCCGCCCGTGCAGGCCCTGTGCGTCGCGCCCTTCGGCATGGAAGAAGGCTCCGA
GGCACCGCTGCCTCCGCAGCAACTGGGCCTCGTGGTCGGTGAATCCGTGCGCTTCCGCTTCTTCGGATCGTCCGTGCGGC
GCGACGACCAGCCCGGCACCCTGCTGGATTTTTGGTCGCCCGAAGAACTGCAGGAACTCGCGCAGATCGAAGCCACGCTG
CCCGCCGAAGGCCGCGCCGCGGGCGAGGTCGTGCCCGTGCAACTGCGCGCGCGCGTGACCGACATCGGCACGCTGGAACT
GCTGGCACAAGCAAGTGTCGGGGCCTCGGGCGGCGCGCAGTGGAAGGTGGAGTTCGACGTCCGCGACGCGTGA

Upstream 100 bases:

>100_bases
GCACGCTGCAGCACAAGGGCTGGCGCGCCACCGCCGTGCGCCTGCCCGCCCTCACCGAAGGGCACGACACCCGCGTGATC
GCCCCCGCGGAGGTCGAACT

Downstream 100 bases:

>100_bases
TGGCGGCCGCCTTCCGCATCGGCATCGACCTGGGCACGACCCACACCGTGCTCGCGTTCGCGCCCCTGGGCGCGGACGAC
ATCCAGGTCTTCCCCATCCC

Product: putative DnaK-like protein

Products: NA

Alternate protein names: HSP70; Heat shock 70 kDa protein; Heat shock protein 70 [H]

Number of amino acids: Translated: 610; Mature: 609

Protein sequence:

>610_residues
MSQPKYAIGIDLGTTHSALSWVDLHTEGAAPEILPVAQQSGPGAVQDYSLLPSFLYVPHDSEFAPGDLALPWNAAPGRIT
GEWARSHGALTPIRLVSSAKSWLGHAGVDRRAGILPADAPPEVERISPLAASTHYLQHLRDAWNHRHPEAPFDQQSVTVT
IPASFDPAARELTAEAAQAAGYHGITLLEEPQAALYSWIAGSAGDWRKQVQVGDVILVVDVGGGTSDFSLIAVTEDAGNL
QLNRVAVGEHILLGGDNMDLALAHGVARGLAAEGKQLDPWQMRALTYACRLAKEQLLSDPGLQSVPLVVPSRGSKLIGGS
IRTELQRELLSTILLEGFFPAVESSARPITRPRAGLTQIGLPYAQDAAVTRHLAALLGRQVGATAQLPGFAQPEGATFLH
PTAVLFNGGVFKSELLAERVLSTLNAWLAADGAPPARLLQGADMDHAVARGAAYYGQVRQGRGIRIRGGTAQAYYVGIES
SMPAVPGLEPPVQALCVAPFGMEEGSEAPLPPQQLGLVVGESVRFRFFGSSVRRDDQPGTLLDFWSPEELQELAQIEATL
PAEGRAAGEVVPVQLRARVTDIGTLELLAQASVGASGGAQWKVEFDVRDA

Sequences:

>Translated_610_residues
MSQPKYAIGIDLGTTHSALSWVDLHTEGAAPEILPVAQQSGPGAVQDYSLLPSFLYVPHDSEFAPGDLALPWNAAPGRIT
GEWARSHGALTPIRLVSSAKSWLGHAGVDRRAGILPADAPPEVERISPLAASTHYLQHLRDAWNHRHPEAPFDQQSVTVT
IPASFDPAARELTAEAAQAAGYHGITLLEEPQAALYSWIAGSAGDWRKQVQVGDVILVVDVGGGTSDFSLIAVTEDAGNL
QLNRVAVGEHILLGGDNMDLALAHGVARGLAAEGKQLDPWQMRALTYACRLAKEQLLSDPGLQSVPLVVPSRGSKLIGGS
IRTELQRELLSTILLEGFFPAVESSARPITRPRAGLTQIGLPYAQDAAVTRHLAALLGRQVGATAQLPGFAQPEGATFLH
PTAVLFNGGVFKSELLAERVLSTLNAWLAADGAPPARLLQGADMDHAVARGAAYYGQVRQGRGIRIRGGTAQAYYVGIES
SMPAVPGLEPPVQALCVAPFGMEEGSEAPLPPQQLGLVVGESVRFRFFGSSVRRDDQPGTLLDFWSPEELQELAQIEATL
PAEGRAAGEVVPVQLRARVTDIGTLELLAQASVGASGGAQWKVEFDVRDA
>Mature_609_residues
SQPKYAIGIDLGTTHSALSWVDLHTEGAAPEILPVAQQSGPGAVQDYSLLPSFLYVPHDSEFAPGDLALPWNAAPGRITG
EWARSHGALTPIRLVSSAKSWLGHAGVDRRAGILPADAPPEVERISPLAASTHYLQHLRDAWNHRHPEAPFDQQSVTVTI
PASFDPAARELTAEAAQAAGYHGITLLEEPQAALYSWIAGSAGDWRKQVQVGDVILVVDVGGGTSDFSLIAVTEDAGNLQ
LNRVAVGEHILLGGDNMDLALAHGVARGLAAEGKQLDPWQMRALTYACRLAKEQLLSDPGLQSVPLVVPSRGSKLIGGSI
RTELQRELLSTILLEGFFPAVESSARPITRPRAGLTQIGLPYAQDAAVTRHLAALLGRQVGATAQLPGFAQPEGATFLHP
TAVLFNGGVFKSELLAERVLSTLNAWLAADGAPPARLLQGADMDHAVARGAAYYGQVRQGRGIRIRGGTAQAYYVGIESS
MPAVPGLEPPVQALCVAPFGMEEGSEAPLPPQQLGLVVGESVRFRFFGSSVRRDDQPGTLLDFWSPEELQELAQIEATLP
AEGRAAGEVVPVQLRARVTDIGTLELLAQASVGASGGAQWKVEFDVRDA

Specific function: Acts as a chaperone [H]

COG id: COG0443

COG function: function code O; Molecular chaperone

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the heat shock protein 70 family [H]

Homologues:

Organism=Escherichia coli, GI1788875, Length=272, Percent_Identity=30.5147058823529, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI1786196, Length=308, Percent_Identity=25.974025974026, Blast_Score=67, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17534015, Length=318, Percent_Identity=27.3584905660377, Blast_Score=75, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17534013, Length=318, Percent_Identity=27.3584905660377, Blast_Score=75, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6324120, Length=312, Percent_Identity=26.6025641025641, Blast_Score=70, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6319972, Length=312, Percent_Identity=26.6025641025641, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012725
- InterPro:   IPR018181
- InterPro:   IPR001023
- InterPro:   IPR013126 [H]

Pfam domain/function: PF00012 HSP70 [H]

EC number: NA

Molecular weight: Translated: 64640; Mature: 64509

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00297 HSP70_1 ; PS00329 HSP70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQPKYAIGIDLGTTHSALSWVDLHTEGAAPEILPVAQQSGPGAVQDYSLLPSFLYVPHD
CCCCCEEEEEECCCCCCCEEEEEEECCCCCCCEEEECCCCCCCCCCHHHHCCCEEECCCC
SEFAPGDLALPWNAAPGRITGEWARSHGALTPIRLVSSAKSWLGHAGVDRRAGILPADAP
CCCCCCCEECCCCCCCCCEEHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
PEVERISPLAASTHYLQHLRDAWNHRHPEAPFDQQSVTVTIPASFDPAARELTAEAAQAA
CCHHHHCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHC
GYHGITLLEEPQAALYSWIAGSAGDWRKQVQVGDVILVVDVGGGTSDFSLIAVTEDAGNL
CCCCEEEECCCHHHHHHHHCCCCCCHHHHEEECCEEEEEECCCCCCCEEEEEEECCCCCE
QLNRVAVGEHILLGGDNMDLALAHGVARGLAAEGKQLDPWQMRALTYACRLAKEQLLSDP
EEEEEEECCEEEECCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
GLQSVPLVVPSRGSKLIGGSIRTELQRELLSTILLEGFFPAVESSARPITRPRAGLTQIG
CCCCCCEEECCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHCC
LPYAQDAAVTRHLAALLGRQVGATAQLPGFAQPEGATFLHPTAVLFNGGVFKSELLAERV
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCHHEEECCCCHHHHHHHHHH
LSTLNAWLAADGAPPARLLQGADMDHAVARGAAYYGQVRQGRGIRIRGGTAQAYYVGIES
HHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEEEECC
SMPAVPGLEPPVQALCVAPFGMEEGSEAPLPPQQLGLVVGESVRFRFFGSSVRRDDQPGT
CCCCCCCCCCCHHHEEECCCCCCCCCCCCCCHHHHHHEECCCEEEEEECCHHCCCCCCCC
LLDFWSPEELQELAQIEATLPAEGRAAGEVVPVQLRARVTDIGTLELLAQASVGASGGAQ
EEECCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEECCCHHHHHHHHHCCCCCCCCE
WKVEFDVRDA
EEEEEEECCC
>Mature Secondary Structure 
SQPKYAIGIDLGTTHSALSWVDLHTEGAAPEILPVAQQSGPGAVQDYSLLPSFLYVPHD
CCCCEEEEEECCCCCCCEEEEEEECCCCCCCEEEECCCCCCCCCCHHHHCCCEEECCCC
SEFAPGDLALPWNAAPGRITGEWARSHGALTPIRLVSSAKSWLGHAGVDRRAGILPADAP
CCCCCCCEECCCCCCCCCEEHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
PEVERISPLAASTHYLQHLRDAWNHRHPEAPFDQQSVTVTIPASFDPAARELTAEAAQAA
CCHHHHCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHC
GYHGITLLEEPQAALYSWIAGSAGDWRKQVQVGDVILVVDVGGGTSDFSLIAVTEDAGNL
CCCCEEEECCCHHHHHHHHCCCCCCHHHHEEECCEEEEEECCCCCCCEEEEEEECCCCCE
QLNRVAVGEHILLGGDNMDLALAHGVARGLAAEGKQLDPWQMRALTYACRLAKEQLLSDP
EEEEEEECCEEEECCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
GLQSVPLVVPSRGSKLIGGSIRTELQRELLSTILLEGFFPAVESSARPITRPRAGLTQIG
CCCCCCEEECCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHCC
LPYAQDAAVTRHLAALLGRQVGATAQLPGFAQPEGATFLHPTAVLFNGGVFKSELLAERV
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCHHEEECCCCHHHHHHHHHH
LSTLNAWLAADGAPPARLLQGADMDHAVARGAAYYGQVRQGRGIRIRGGTAQAYYVGIES
HHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEEEECC
SMPAVPGLEPPVQALCVAPFGMEEGSEAPLPPQQLGLVVGESVRFRFFGSSVRRDDQPGT
CCCCCCCCCCCHHHEEECCCCCCCCCCCCCCHHHHHHEECCCEEEEEECCHHCCCCCCCC
LLDFWSPEELQELAQIEATLPAEGRAAGEVVPVQLRARVTDIGTLELLAQASVGASGGAQ
EEECCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEECCCHHHHHHHHHCCCCCCCCE
WKVEFDVRDA
EEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12835416 [H]