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The map label for this gene is proB

Identifier: 120612316

GI number: 120612316

Start: 4049288

End: 4050427

Strand: Reverse

Name: proB

Synonym: Aave_3672

Alternate gene names: 120612316

Gene position: 4050427-4049288 (Counterclockwise)

Preceding gene: 120612317

Following gene: 120612314

Centisome position: 75.67

GC content: 69.82

Gene sequence:

>1140_bases
ATGGTTTCGAGCATATTGCGAGATGCGCGCCGCATCGTGATCAAGGTGGGCTCCAGCCTGGTGACGAACGAGGGCCGGGG
GCTGGACGAGACGGCCATCACCGAATGGAGCCGGCAGATGGCCGCGCTGGTGAACGGATCCGGCGGGCCGCGGCGCGAGG
TCATCATGGTGTCCAGCGGCGCGATCGCCGAGGGCATGAAGCGCCTGGGCTGGTCCGCGCGCCCGAGCGAGATCCACGAA
TTGCAGGCGGCAGCTGCCGTGGGCCAGATGGGGCTCGCGCAGATGTACGAGACCAAGCTGCGCGAACAGGGCCTGGGCAG
TGCCCAGGTGCTGCTGACCCATGCGGACCTCGCGGACCGCGAGCGCTACCTGAACGCGCGCTCCACGCTCCTCACGCTGC
TGCGGCTCGGCGTGGTGCCCGTCATCAACGAGAACGACACGGTGGTCACCGACGAGATCAAGTTCGGCGACAACGACACC
CTTGGCGCGCTGGTTGCGAACCTCGTGGAAGCCGATGCGCTGGTCATCCTGACGGACCAGAAGGGCCTCTACACGGCGGA
CCCGCGGCGCGATCCTCATGCGCAGTTCGTGCACGAGGCGGCGGCCGGCGATCCTTCCCTGGAGGCGATGGCGGGCGGCG
TGGGCACCAGCATCGGACGCGGGGGCATGATCACCAAGATCATCGCGGCCAGGCGTGCGGCCGGCTCGGGGGCTTCCACG
GTGATCGCCTGGGGGCGCGAGCCGGACGTGCTGCTGCGGCTCACGCAGGGCGAGGCCATCGGCACCCTGCTGGTGGCGCA
GACCGCCAAGAAGCAGGCCCGCAAGCAGTGGATGGCGGACCACCTCCAGCTGCGCGGCGCCGTGACGGTGGATGTCGGCG
CGGCGGCCAAGCTGCGGCAGGAAGGAAAGAGCCTCCTACCGATCGGCATGGTTGCGGTGGAGGGCGAGTTCTCCCGGGGC
GACGTGATCGCCGTGCGCGACGCGGCGGGAGCGGAAATCGCCCGCGGGCTCGCGAACTACGCCAGCGCCGAGGCGCGGCT
GCTGTGCCGCCGGCCGTCTTCGGAATTCGAGCGGCTGCTGGGCTACAGCGCCGAACCGGAAATGGTGCACCGTGACAACC
TCGTGCTGTCCGGCGGCTGA

Upstream 100 bases:

>100_bases
GACGACGCGGCTTCGGATTGACGGGCAGGGCCGCCATCCCCGGTGGGTGGCGGCGTGGGGCCGCTGCGGCGGCCGGCGCT
ATGAAAGAAGACGGGGATAC

Downstream 100 bases:

>100_bases
GCCGGCAGCATCGCAAAAAAAGGGCGCTCCAGGGAGCGCCTTTTTTTTGCGTGGTCGTTTCAGCCGGCCGGTTTGTGGAT
GCCGGGCATGCCCGGGTCGC

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MVSSILRDARRIVIKVGSSLVTNEGRGLDETAITEWSRQMAALVNGSGGPRREVIMVSSGAIAEGMKRLGWSARPSEIHE
LQAAAAVGQMGLAQMYETKLREQGLGSAQVLLTHADLADRERYLNARSTLLTLLRLGVVPVINENDTVVTDEIKFGDNDT
LGALVANLVEADALVILTDQKGLYTADPRRDPHAQFVHEAAAGDPSLEAMAGGVGTSIGRGGMITKIIAARRAAGSGAST
VIAWGREPDVLLRLTQGEAIGTLLVAQTAKKQARKQWMADHLQLRGAVTVDVGAAAKLRQEGKSLLPIGMVAVEGEFSRG
DVIAVRDAAGAEIARGLANYASAEARLLCRRPSSEFERLLGYSAEPEMVHRDNLVLSGG

Sequences:

>Translated_379_residues
MVSSILRDARRIVIKVGSSLVTNEGRGLDETAITEWSRQMAALVNGSGGPRREVIMVSSGAIAEGMKRLGWSARPSEIHE
LQAAAAVGQMGLAQMYETKLREQGLGSAQVLLTHADLADRERYLNARSTLLTLLRLGVVPVINENDTVVTDEIKFGDNDT
LGALVANLVEADALVILTDQKGLYTADPRRDPHAQFVHEAAAGDPSLEAMAGGVGTSIGRGGMITKIIAARRAAGSGAST
VIAWGREPDVLLRLTQGEAIGTLLVAQTAKKQARKQWMADHLQLRGAVTVDVGAAAKLRQEGKSLLPIGMVAVEGEFSRG
DVIAVRDAAGAEIARGLANYASAEARLLCRRPSSEFERLLGYSAEPEMVHRDNLVLSGG
>Mature_379_residues
MVSSILRDARRIVIKVGSSLVTNEGRGLDETAITEWSRQMAALVNGSGGPRREVIMVSSGAIAEGMKRLGWSARPSEIHE
LQAAAAVGQMGLAQMYETKLREQGLGSAQVLLTHADLADRERYLNARSTLLTLLRLGVVPVINENDTVVTDEIKFGDNDT
LGALVANLVEADALVILTDQKGLYTADPRRDPHAQFVHEAAAGDPSLEAMAGGVGTSIGRGGMITKIIAARRAAGSGAST
VIAWGREPDVLLRLTQGEAIGTLLVAQTAKKQARKQWMADHLQLRGAVTVDVGAAAKLRQEGKSLLPIGMVAVEGEFSRG
DVIAVRDAAGAEIARGLANYASAEARLLCRRPSSEFERLLGYSAEPEMVHRDNLVLSGG

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Homo sapiens, GI21361368, Length=296, Percent_Identity=32.0945945945946, Blast_Score=125, Evalue=9e-29,
Organism=Homo sapiens, GI62912457, Length=294, Percent_Identity=32.312925170068, Blast_Score=124, Evalue=1e-28,
Organism=Escherichia coli, GI1786437, Length=370, Percent_Identity=44.0540540540541, Blast_Score=280, Evalue=1e-76,
Organism=Caenorhabditis elegans, GI17569967, Length=276, Percent_Identity=32.6086956521739, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI17569969, Length=276, Percent_Identity=32.6086956521739, Blast_Score=124, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6320506, Length=421, Percent_Identity=32.3040380047506, Blast_Score=188, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6321822, Length=423, Percent_Identity=31.2056737588652, Blast_Score=177, Evalue=3e-45,
Organism=Drosophila melanogaster, GI21357643, Length=303, Percent_Identity=32.3432343234323, Blast_Score=127, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROB_ACIAC (A1TTD2)

Other databases:

- EMBL:   CP000512
- RefSeq:   YP_971994.1
- ProteinModelPortal:   A1TTD2
- STRING:   A1TTD2
- GeneID:   4669177
- GenomeReviews:   CP000512_GR
- KEGG:   aav:Aave_3672
- NMPDR:   fig|397945.5.peg.3136
- eggNOG:   COG0263
- HOGENOM:   HBG507643
- OMA:   TFGDNDM
- PhylomeDB:   A1TTD2
- ProtClustDB:   PRK05429
- BioCyc:   AAVE397945:AAVE_3672-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00456
- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000729
- PRINTS:   PR00474
- SMART:   SM00359
- TIGRFAMs:   TIGR01027

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA; SSF53633 Aa_kinase; SSF88697 PUA-like

EC number: =2.7.2.11

Molecular weight: Translated: 40230; Mature: 40230

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS00902 GLUTAMATE_5_KINASE; PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSSILRDARRIVIKVGSSLVTNEGRGLDETAITEWSRQMAALVNGSGGPRREVIMVSSG
CHHHHHHHHHHHHHHHCCHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEECC
AIAEGMKRLGWSARPSEIHELQAAAAVGQMGLAQMYETKLREQGLGSAQVLLTHADLADR
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHH
ERYLNARSTLLTLLRLGVVPVINENDTVVTDEIKFGDNDTLGALVANLVEADALVILTDQ
HHHHHHHHHHHHHHHHCCEEEECCCCEEEEEEEECCCCCHHHHHHHHHHCCCEEEEEECC
KGLYTADPRRDPHAQFVHEAAAGDPSLEAMAGGVGTSIGRGGMITKIIAARRAAGSGAST
CCCEECCCCCCCHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCE
VIAWGREPDVLLRLTQGEAIGTLLVAQTAKKQARKQWMADHLQLRGAVTVDVGAAAKLRQ
EEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHH
EGKSLLPIGMVAVEGEFSRGDVIAVRDAAGAEIARGLANYASAEARLLCRRPSSEFERLL
CCCCCCEEEEEEEECCCCCCCEEEEECCCHHHHHHHHHHHHCHHHHHEECCCCHHHHHHH
GYSAEPEMVHRDNLVLSGG
CCCCCCCEEECCCEEEECC
>Mature Secondary Structure
MVSSILRDARRIVIKVGSSLVTNEGRGLDETAITEWSRQMAALVNGSGGPRREVIMVSSG
CHHHHHHHHHHHHHHHCCHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEECC
AIAEGMKRLGWSARPSEIHELQAAAAVGQMGLAQMYETKLREQGLGSAQVLLTHADLADR
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHH
ERYLNARSTLLTLLRLGVVPVINENDTVVTDEIKFGDNDTLGALVANLVEADALVILTDQ
HHHHHHHHHHHHHHHHCCEEEECCCCEEEEEEEECCCCCHHHHHHHHHHCCCEEEEEECC
KGLYTADPRRDPHAQFVHEAAAGDPSLEAMAGGVGTSIGRGGMITKIIAARRAAGSGAST
CCCEECCCCCCCHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCE
VIAWGREPDVLLRLTQGEAIGTLLVAQTAKKQARKQWMADHLQLRGAVTVDVGAAAKLRQ
EEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHH
EGKSLLPIGMVAVEGEFSRGDVIAVRDAAGAEIARGLANYASAEARLLCRRPSSEFERLL
CCCCCCEEEEEEEECCCCCCCEEEEECCCHHHHHHHHHHHHCHHHHHEECCCCHHHHHHH
GYSAEPEMVHRDNLVLSGG
CCCCCCCEEECCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA