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The map label for this gene is proS

Identifier: 120612314

GI number: 120612314

Start: 4046710

End: 4048455

Strand: Reverse

Name: proS

Synonym: Aave_3670

Alternate gene names: 120612314

Gene position: 4048455-4046710 (Counterclockwise)

Preceding gene: 120612316

Following gene: 120612313

Centisome position: 75.63

GC content: 67.24

Gene sequence:

>1746_bases
ATGAAAGCTTCCCAATTTTTCGTTTCCACCCTCAAGGAAGCCCCGGCCGACGCCGAGGTGGTGAGCCACAAGCTCATGAC
GCGCGCCGGACTCATCAAGAAGCTGGGCGCCGGCATCTACAACTACATGCCCATGGGCCTGCGCGTGATCCGGAAGGTGG
AAGCCATCGTGCGCGAGGAAATGAACCGCGCCGGTGCCGTCGAGGTCACGATGCCGGTGGTGCAGCCGGCGGAGTACTGG
CAGGAAACGGGCCGTTTCGACAAGATGGGCCCCGAGCTGCTGCGCATCCGGGACCGCCACGGCCGCGATTTCGTCGTGCA
GCCGACCAGCGAGGAAGTGGTCACTGACATCGCGCGCCAGGAACTGCGCAGCTACAAGCAGCTTCCCAAGAACCTCTACC
AGATCCAGACCAAGTTCCGCGACGAGCGCCGTCCGCGCTTCGGGCTGATGCGCGGCCGCGAATTCATCATGAAGGACGCC
TATTCCTTCGACCGCGACCAGGCCGCCGCCAAGGCCAGCTACCAGGTAATGGCGCAGGCCTACCGGCGCATCTTCGACCG
CTTTGGCCTGACCTACCGCGCGGTGGCCGCCGACAGCGGCGCGATCGGTGGCGACCTGAGCGAGGAATTCCAGGTGATCG
CCGCCACGGGCGAGGATGCGATCGTCTATTGCCCGCAGAGCGACTACGCGGCGAACATGGAAAAGGCCGAGGCGCTGCCG
CCGCAGGGCCCGCGCCCCGCGGCTTCGCAGGCGCTCGCCAGGACCGCCACGCCCGGCAAGAGCACCTGCGCCGACGTCGC
GCAACTGCTCGGCGTGCCGCTGCAGGCGACCGTGAAATCGCTGGTGCTCGCGACCGATGAAACGAACGAGTCCGGCGAGA
TCGTCCGCAGCCAGGTCTGGCTGCTGCTGCTGCGCGGCGACCATGACATGAACGAGATCAAGGTCGGCAAGGTGCCAGGC
CTGGATGCGGGCTTCCGCTTCGCCACCGTGGGCGAGATCGAAGACCATTTCGGCTGCAGGCCGGGCTACCTGGGCCCGCT
GAACCTGCGCCAGCCCGTCAGGCTCGTGGTGGACCGCGAGGTGGCGGTGATGGCCGACTGGATCTGCGGCGCCAACGAGG
TGGATTTCCACATGACCGGCGTGAACTGGGGCCGCGACCTGCCCGAGCCGGACGTGGTGGCAGACCTGCGCAACGTCGTG
GCCGGCGATCCGTCGCCGGACGGCAAGGGAACGCTGGCGATCGAGCGCGGCATCGAAGTGGGCCACGTGTTCTACCTGGG
CACCAAGTACAGCCGCGCCATGAACGCGACCTTCCTCGGCGAGGATGGCAAGCCGGCCTTTTTCGAGATGGGCTGCTACG
GCATCGGCATCACGCGGCTGCCCGCGGCGGCCATCGAGCAGAACCACGACGAGCGTGGCATCATCTGGCCCGATGCGATC
GCGCCCTTCACGGTCGTGGTGTGCCCGATCGGCATGGACCGCAGCGACGAGGTGCGCGCTGCGGCCGAGAAGCTGCATGC
CGACCTGCTCACGGCCGGCGTGGACGTGATCCTGGACGACCGCGGCGAGCGCCCGGGCGCGATGTTCGCCGACTGGGAAC
TCATCGGCGTGCCGCACCGCGTGGTGCTGTCCGACCGCGGCCTCAAGGAGGGCCAGGTCGAGTACCAGCACCGCCGGGAT
GCGGCGGCGACCAAGGTGGCGTCGGCCGATATCTTTGCCTTCATCAAGGATCGAATCAAGGTATGA

Upstream 100 bases:

>100_bases
TCGAGCATAATCTAACCCCAATTTTTAAAACTGTATCCATTATGCACGGGCATGGGTGCGCGGCAAGCGTACCGGCCCGG
ATGGTGAATTCCCTCGTGCA

Downstream 100 bases:

>100_bases
ACTGGGGTGGTGGGGTGAGGGGCGGGACGCCCGGCAGGGGCCTGTCCCGGCGTGCGTGCGTGCTGGCCGGCGCGTCTTCA
GGATTGGCCGGGTGGCTCGG

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 581; Mature: 581

Protein sequence:

>581_residues
MKASQFFVSTLKEAPADAEVVSHKLMTRAGLIKKLGAGIYNYMPMGLRVIRKVEAIVREEMNRAGAVEVTMPVVQPAEYW
QETGRFDKMGPELLRIRDRHGRDFVVQPTSEEVVTDIARQELRSYKQLPKNLYQIQTKFRDERRPRFGLMRGREFIMKDA
YSFDRDQAAAKASYQVMAQAYRRIFDRFGLTYRAVAADSGAIGGDLSEEFQVIAATGEDAIVYCPQSDYAANMEKAEALP
PQGPRPAASQALARTATPGKSTCADVAQLLGVPLQATVKSLVLATDETNESGEIVRSQVWLLLLRGDHDMNEIKVGKVPG
LDAGFRFATVGEIEDHFGCRPGYLGPLNLRQPVRLVVDREVAVMADWICGANEVDFHMTGVNWGRDLPEPDVVADLRNVV
AGDPSPDGKGTLAIERGIEVGHVFYLGTKYSRAMNATFLGEDGKPAFFEMGCYGIGITRLPAAAIEQNHDERGIIWPDAI
APFTVVVCPIGMDRSDEVRAAAEKLHADLLTAGVDVILDDRGERPGAMFADWELIGVPHRVVLSDRGLKEGQVEYQHRRD
AAATKVASADIFAFIKDRIKV

Sequences:

>Translated_581_residues
MKASQFFVSTLKEAPADAEVVSHKLMTRAGLIKKLGAGIYNYMPMGLRVIRKVEAIVREEMNRAGAVEVTMPVVQPAEYW
QETGRFDKMGPELLRIRDRHGRDFVVQPTSEEVVTDIARQELRSYKQLPKNLYQIQTKFRDERRPRFGLMRGREFIMKDA
YSFDRDQAAAKASYQVMAQAYRRIFDRFGLTYRAVAADSGAIGGDLSEEFQVIAATGEDAIVYCPQSDYAANMEKAEALP
PQGPRPAASQALARTATPGKSTCADVAQLLGVPLQATVKSLVLATDETNESGEIVRSQVWLLLLRGDHDMNEIKVGKVPG
LDAGFRFATVGEIEDHFGCRPGYLGPLNLRQPVRLVVDREVAVMADWICGANEVDFHMTGVNWGRDLPEPDVVADLRNVV
AGDPSPDGKGTLAIERGIEVGHVFYLGTKYSRAMNATFLGEDGKPAFFEMGCYGIGITRLPAAAIEQNHDERGIIWPDAI
APFTVVVCPIGMDRSDEVRAAAEKLHADLLTAGVDVILDDRGERPGAMFADWELIGVPHRVVLSDRGLKEGQVEYQHRRD
AAATKVASADIFAFIKDRIKV
>Mature_581_residues
MKASQFFVSTLKEAPADAEVVSHKLMTRAGLIKKLGAGIYNYMPMGLRVIRKVEAIVREEMNRAGAVEVTMPVVQPAEYW
QETGRFDKMGPELLRIRDRHGRDFVVQPTSEEVVTDIARQELRSYKQLPKNLYQIQTKFRDERRPRFGLMRGREFIMKDA
YSFDRDQAAAKASYQVMAQAYRRIFDRFGLTYRAVAADSGAIGGDLSEEFQVIAATGEDAIVYCPQSDYAANMEKAEALP
PQGPRPAASQALARTATPGKSTCADVAQLLGVPLQATVKSLVLATDETNESGEIVRSQVWLLLLRGDHDMNEIKVGKVPG
LDAGFRFATVGEIEDHFGCRPGYLGPLNLRQPVRLVVDREVAVMADWICGANEVDFHMTGVNWGRDLPEPDVVADLRNVV
AGDPSPDGKGTLAIERGIEVGHVFYLGTKYSRAMNATFLGEDGKPAFFEMGCYGIGITRLPAAAIEQNHDERGIIWPDAI
APFTVVVCPIGMDRSDEVRAAAEKLHADLLTAGVDVILDDRGERPGAMFADWELIGVPHRVVLSDRGLKEGQVEYQHRRD
AAATKVASADIFAFIKDRIKV

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=249, Percent_Identity=44.578313253012, Blast_Score=223, Evalue=4e-58,
Organism=Escherichia coli, GI1786392, Length=580, Percent_Identity=54.6551724137931, Blast_Score=663, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115532348, Length=244, Percent_Identity=36.8852459016393, Blast_Score=171, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI193203271, Length=90, Percent_Identity=33.3333333333333, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6320931, Length=223, Percent_Identity=34.5291479820628, Blast_Score=174, Evalue=3e-44,
Organism=Drosophila melanogaster, GI24656200, Length=256, Percent_Identity=38.671875, Blast_Score=184, Evalue=2e-46,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_ACIAC (A1TTD0)

Other databases:

- EMBL:   CP000512
- RefSeq:   YP_971992.1
- ProteinModelPortal:   A1TTD0
- SMR:   A1TTD0
- STRING:   A1TTD0
- GeneID:   4665262
- GenomeReviews:   CP000512_GR
- KEGG:   aav:Aave_3670
- NMPDR:   fig|397945.5.peg.3134
- eggNOG:   COG0442
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- PhylomeDB:   A1TTD0
- ProtClustDB:   PRK09194
- BioCyc:   AAVE397945:AAVE_3670-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01569
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.90.960.10
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK; SSF52954 Anticodon_bd; SSF55826 YbaK/aa-tRNA-synth-assoc-reg

EC number: =6.1.1.15

Molecular weight: Translated: 64157; Mature: 64157

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKASQFFVSTLKEAPADAEVVSHKLMTRAGLIKKLGAGIYNYMPMGLRVIRKVEAIVREE
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
MNRAGAVEVTMPVVQPAEYWQETGRFDKMGPELLRIRDRHGRDFVVQPTSEEVVTDIARQ
HHCCCCEEEEECCCCHHHHHHHCCCCCCCCHHHHHHHHCCCCEEEECCCHHHHHHHHHHH
ELRSYKQLPKNLYQIQTKFRDERRPRFGLMRGREFIMKDAYSFDRDQAAAKASYQVMAQA
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
YRRIFDRFGLTYRAVAADSGAIGGDLSEEFQVIAATGEDAIVYCPQSDYAANMEKAEALP
HHHHHHHHCCEEEEEECCCCCCCCCCCCCCEEEEECCCCEEEECCCCCCCCCCHHHHCCC
PQGPRPAASQALARTATPGKSTCADVAQLLGVPLQATVKSLVLATDETNESGEIVRSQVW
CCCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHEEECCCCCCCHHHHHHEEE
LLLLRGDHDMNEIKVGKVPGLDAGFRFATVGEIEDHFGCRPGYLGPLNLRQPVRLVVDRE
EEEEECCCCCCCEEECCCCCCCCCCEEEEECCHHHHCCCCCCCCCCCCCCCCHHHHCCCH
VAVMADWICGANEVDFHMTGVNWGRDLPEPDVVADLRNVVAGDPSPDGKGTLAIERGIEV
HHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCE
GHVFYLGTKYSRAMNATFLGEDGKPAFFEMGCYGIGITRLPAAAIEQNHDERGIIWPDAI
EEEEEECCHHHHHCCCEEECCCCCCCEEECCCEECCHHHCCHHHHHCCCCCCCCCCCCCC
APFTVVVCPIGMDRSDEVRAAAEKLHADLLTAGVDVILDDRGERPGAMFADWELIGVPHR
CCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCEEECEEEECCCCE
VVLSDRGLKEGQVEYQHRRDAAATKVASADIFAFIKDRIKV
EEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKASQFFVSTLKEAPADAEVVSHKLMTRAGLIKKLGAGIYNYMPMGLRVIRKVEAIVREE
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
MNRAGAVEVTMPVVQPAEYWQETGRFDKMGPELLRIRDRHGRDFVVQPTSEEVVTDIARQ
HHCCCCEEEEECCCCHHHHHHHCCCCCCCCHHHHHHHHCCCCEEEECCCHHHHHHHHHHH
ELRSYKQLPKNLYQIQTKFRDERRPRFGLMRGREFIMKDAYSFDRDQAAAKASYQVMAQA
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
YRRIFDRFGLTYRAVAADSGAIGGDLSEEFQVIAATGEDAIVYCPQSDYAANMEKAEALP
HHHHHHHHCCEEEEEECCCCCCCCCCCCCCEEEEECCCCEEEECCCCCCCCCCHHHHCCC
PQGPRPAASQALARTATPGKSTCADVAQLLGVPLQATVKSLVLATDETNESGEIVRSQVW
CCCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHEEECCCCCCCHHHHHHEEE
LLLLRGDHDMNEIKVGKVPGLDAGFRFATVGEIEDHFGCRPGYLGPLNLRQPVRLVVDRE
EEEEECCCCCCCEEECCCCCCCCCCEEEEECCHHHHCCCCCCCCCCCCCCCCHHHHCCCH
VAVMADWICGANEVDFHMTGVNWGRDLPEPDVVADLRNVVAGDPSPDGKGTLAIERGIEV
HHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCE
GHVFYLGTKYSRAMNATFLGEDGKPAFFEMGCYGIGITRLPAAAIEQNHDERGIIWPDAI
EEEEEECCHHHHHCCCEEECCCCCCCEEECCCEECCHHHCCHHHHHCCCCCCCCCCCCCC
APFTVVVCPIGMDRSDEVRAAAEKLHADLLTAGVDVILDDRGERPGAMFADWELIGVPHR
CCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCEEECEEEECCCCE
VVLSDRGLKEGQVEYQHRRDAAATKVASADIFAFIKDRIKV
EEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA