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The map label for this gene is pinE [H]

Identifier: 120612241

GI number: 120612241

Start: 3964793

End: 3965386

Strand: Reverse

Name: pinE [H]

Synonym: Aave_3594

Alternate gene names: 120612241

Gene position: 3965386-3964793 (Counterclockwise)

Preceding gene: 120612242

Following gene: 120612240

Centisome position: 74.08

GC content: 61.95

Gene sequence:

>594_bases
ATGGGGAGACTGGTCGGGTACGCCCGCGTCAGCACGCGCGAGCAGGAAACGGTGATGCAGTTGGCCGCGCTGCGTGCTGC
GGGTGTTGGGGTTGTCTACGAAGAGCAGGCCAGTGCGGTCCGTCATCGGCCAGTGTGGGAGCGATGTCTTGCGGCGCTCA
AAAAGGGGGATGTGCTGGTGGTTTACAAGCTGGATCGTCTCGCTCGATCTACCTCCCACTTTGTCACTACGTTTGACTTG
CTCAGGTCGCGCGGTGTGGGGTTCAGAAGCCTGACGGAAGCGATTGAGACAGTCACCCCGCAAGGTCGTATGTACCTGCA
GCTACTGGCAGTCTTTGCCGAGTTTGAAAGGGAGTTGATCCGGGAACGCTGCGTCGCTGGGCAGCGTGCCGCGCGTGCGG
CTGGGAAGACGTGGGGGCGTAAGGCCCAGTTGTCGCGGGATGAGGTTGCTCAGGCCGTGCAGGCGTGGCGAAGTGGGTGG
TACACACAGGATGTTTTGGCCGACATGTTGGGTGTGCCTGTCGGCACGTTGCGATACCACGTCCACCGCCATGAGGGTCG
CGGCCGGCACGGCGCATGGAGCACAGATAAGTAA

Upstream 100 bases:

>100_bases
TGTATTTCGGCTGCACCAAGGCCGATGTAGTGCGGTCGATGCTCCGCTATGCGGCGACTAACCGGGACTGGAAGAGACAA
GGGTTGATCTGGAGGGATTG

Downstream 100 bases:

>100_bases
AATGATTCAATGCTCGATGACCGTGCCAAGTTGTTGCTCAAAGCGCTGATCGAGCGCTACATCGCCGACGGCCAGCCGGT
GGGATCGCGCACCCTGTCCA

Product: resolvase domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 197; Mature: 196

Protein sequence:

>197_residues
MGRLVGYARVSTREQETVMQLAALRAAGVGVVYEEQASAVRHRPVWERCLAALKKGDVLVVYKLDRLARSTSHFVTTFDL
LRSRGVGFRSLTEAIETVTPQGRMYLQLLAVFAEFERELIRERCVAGQRAARAAGKTWGRKAQLSRDEVAQAVQAWRSGW
YTQDVLADMLGVPVGTLRYHVHRHEGRGRHGAWSTDK

Sequences:

>Translated_197_residues
MGRLVGYARVSTREQETVMQLAALRAAGVGVVYEEQASAVRHRPVWERCLAALKKGDVLVVYKLDRLARSTSHFVTTFDL
LRSRGVGFRSLTEAIETVTPQGRMYLQLLAVFAEFERELIRERCVAGQRAARAAGKTWGRKAQLSRDEVAQAVQAWRSGW
YTQDVLADMLGVPVGTLRYHVHRHEGRGRHGAWSTDK
>Mature_196_residues
GRLVGYARVSTREQETVMQLAALRAAGVGVVYEEQASAVRHRPVWERCLAALKKGDVLVVYKLDRLARSTSHFVTTFDLL
RSRGVGFRSLTEAIETVTPQGRMYLQLLAVFAEFERELIRERCVAGQRAARAAGKTWGRKAQLSRDEVAQAVQAWRSGWY
TQDVLADMLGVPVGTLRYHVHRHEGRGRHGAWSTDK

Specific function: This protein catalyzes the inversion of an 1800-bp E.coli DNA fragment, the P region, which can exist in either orientation. The function of the inversion is not yet clear [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=175, Percent_Identity=44.5714285714286, Blast_Score=137, Evalue=4e-34,
Organism=Escherichia coli, GI1787638, Length=167, Percent_Identity=35.3293413173653, Blast_Score=90, Evalue=9e-20,
Organism=Escherichia coli, GI1787827, Length=167, Percent_Identity=34.7305389221557, Blast_Score=89, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR006118
- InterPro:   IPR006119
- InterPro:   IPR006120 [H]

Pfam domain/function: PF02796 HTH_7; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 22179; Mature: 22048

Theoretical pI: Translated: 10.73; Mature: 10.73

Prosite motif: PS00397 RECOMBINASES_1 ; PS00398 RECOMBINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRLVGYARVSTREQETVMQLAALRAAGVGVVYEEQASAVRHRPVWERCLAALKKGDVLV
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHCCHHHHHHHHHHHCCCEEE
VYKLDRLARSTSHFVTTFDLLRSRGVGFRSLTEAIETVTPQGRMYLQLLAVFAEFERELI
EEEHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
RERCVAGQRAARAAGKTWGRKAQLSRDEVAQAVQAWRSGWYTQDVLADMLGVPVGTLRYH
HHHHHHHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHH
VHRHEGRGRHGAWSTDK
HHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
GRLVGYARVSTREQETVMQLAALRAAGVGVVYEEQASAVRHRPVWERCLAALKKGDVLV
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHCCHHHHHHHHHHHCCCEEE
VYKLDRLARSTSHFVTTFDLLRSRGVGFRSLTEAIETVTPQGRMYLQLLAVFAEFERELI
EEEHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
RERCVAGQRAARAAGKTWGRKAQLSRDEVAQAVQAWRSGWYTQDVLADMLGVPVGTLRYH
HHHHHHHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHH
VHRHEGRGRHGAWSTDK
HHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3894006; 6310572; 8905232; 9278503 [H]