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The map label for this gene is pleD [H]

Identifier: 120612175

GI number: 120612175

Start: 3896501

End: 3897562

Strand: Reverse

Name: pleD [H]

Synonym: Aave_3524

Alternate gene names: 120612175

Gene position: 3897562-3896501 (Counterclockwise)

Preceding gene: 120612176

Following gene: 120612174

Centisome position: 72.81

GC content: 72.22

Gene sequence:

>1062_bases
ATGACCGACGTGCCTTCGCCTTCCGCCCCTCATGCCGAGTCCGCCGGCCGCGCAGCCCCGCTGATGGACAGCGACAGCGC
CGGCCTGGTGCTGATCGTGGACGACGTGCCCGACAACCTCGCCGTGCTGCACGATGCGCTCGACGAGTCGGGCTACACGG
TGCTGGTCGCCACCGGCGGAGAGACCGCGCTGGCCCGCGCCGCCCAGGCCATGCCCGACGTGGTGCTGCTCGACGCCATG
ATGCCCGGCATGGACGGCTTCGAGGTCGCGCGCCGCCTGAAGGCCCGGCCGGCCACCGCGCACATCCCCATCATCTTCAT
GACCGGCCTCACCGAGACCGAGCACCTGGAGGCGGCCCTGGCCGCGGGCGGCGTGGACTACGTGACCAAGCCCATCAAGC
CGCGCGAGGTGCTGGCACGCATGAACGTGCACCTGCAGGGCGCGCGCCGGGCCCGGCAGGACATGCGCCACGCGGGCCAG
GCGCGCAATGCGCTGGATGCCTTCGGCTATGCCAGCATCACCGTGCGCGCGAGCGACGGGCGGCTGATGTGGCAGACCGC
GCTGGCGCGCACGCTGCTGCGCGAATACTTCCCGGCCGAGGGCGACGGGCCGCGCGATCCGGCGGCCACGCCCCAGCCCG
TGGCCGAATGGCTGCGCGCGCTGCTGCCGCAGATACCGGTCTTCGCCGCGCAGCGGACCGAACCGCCGCGCCTGGTGGTG
GAGCGTGGCGCGCGGCGCCTGTCGTTCAGCCTGCACCAGCAGACCGGCGACGGCGAGGGCGGCGGCGACTGGCTGGTCGT
GATGCGCGAGGTGTCCGACGCGGCGGTGATCGAGGCACTGGCCCTGGGTTTTCGGCTCACGGCGCGCGAGGCCGAAGTGC
TCTACTGGCTGCTCAAGGGCAAGACCAACCGCGACATCGGCGACATCCTGGGGTCGAGCCCCGCCACGGTGAAGAAGCAC
CTGGAGCGCGTCTATGTGAAGCTGGGCGCGGAAACCCGCACTGCCGCCGCGGGCATCGCCATGGCGCGCATCCGGCAGAC
ACACCCGCAATTCGAAGGGTAG

Upstream 100 bases:

>100_bases
CGCAGCCCGAATGCGCCGGCTGGGTGGCCCGGGCGCGCGCTCTGGCGCGGCAGTTCCAGTTCGAGGCGCTGGCCCGCCTC
ATGACCTCTTCCTGATCCCG

Downstream 100 bases:

>100_bases
TCCGGGCAGCGGAACGCAGCTTCGGCGCCCTTCTGGGACAATCCGCGACCGCCCGACCGCTGGTTTTCCGCTTTTTTCCG
ATGAACGATCCTGCCCCTTC

Product: two component LuxR family transcriptional regulator

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 353; Mature: 352

Protein sequence:

>353_residues
MTDVPSPSAPHAESAGRAAPLMDSDSAGLVLIVDDVPDNLAVLHDALDESGYTVLVATGGETALARAAQAMPDVVLLDAM
MPGMDGFEVARRLKARPATAHIPIIFMTGLTETEHLEAALAAGGVDYVTKPIKPREVLARMNVHLQGARRARQDMRHAGQ
ARNALDAFGYASITVRASDGRLMWQTALARTLLREYFPAEGDGPRDPAATPQPVAEWLRALLPQIPVFAAQRTEPPRLVV
ERGARRLSFSLHQQTGDGEGGGDWLVVMREVSDAAVIEALALGFRLTAREAEVLYWLLKGKTNRDIGDILGSSPATVKKH
LERVYVKLGAETRTAAAGIAMARIRQTHPQFEG

Sequences:

>Translated_353_residues
MTDVPSPSAPHAESAGRAAPLMDSDSAGLVLIVDDVPDNLAVLHDALDESGYTVLVATGGETALARAAQAMPDVVLLDAM
MPGMDGFEVARRLKARPATAHIPIIFMTGLTETEHLEAALAAGGVDYVTKPIKPREVLARMNVHLQGARRARQDMRHAGQ
ARNALDAFGYASITVRASDGRLMWQTALARTLLREYFPAEGDGPRDPAATPQPVAEWLRALLPQIPVFAAQRTEPPRLVV
ERGARRLSFSLHQQTGDGEGGGDWLVVMREVSDAAVIEALALGFRLTAREAEVLYWLLKGKTNRDIGDILGSSPATVKKH
LERVYVKLGAETRTAAAGIAMARIRQTHPQFEG
>Mature_352_residues
TDVPSPSAPHAESAGRAAPLMDSDSAGLVLIVDDVPDNLAVLHDALDESGYTVLVATGGETALARAAQAMPDVVLLDAMM
PGMDGFEVARRLKARPATAHIPIIFMTGLTETEHLEAALAAGGVDYVTKPIKPREVLARMNVHLQGARRARQDMRHAGQA
RNALDAFGYASITVRASDGRLMWQTALARTLLREYFPAEGDGPRDPAATPQPVAEWLRALLPQIPVFAAQRTEPPRLVVE
RGARRLSFSLHQQTGDGEGGGDWLVVMREVSDAAVIEALALGFRLTAREAEVLYWLLKGKTNRDIGDILGSSPATVKKHL
ERVYVKLGAETRTAAAGIAMARIRQTHPQFEG

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1788394, Length=124, Percent_Identity=33.8709677419355, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788550, Length=135, Percent_Identity=35.5555555555556, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1786599, Length=113, Percent_Identity=30.9734513274336, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1790860, Length=125, Percent_Identity=35.2, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1789809, Length=146, Percent_Identity=31.5068493150685, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1786784, Length=117, Percent_Identity=33.3333333333333, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1790437, Length=104, Percent_Identity=35.5769230769231, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1790299, Length=129, Percent_Identity=33.3333333333333, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI145693157, Length=151, Percent_Identity=27.8145695364238, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 38010; Mature: 37879

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDVPSPSAPHAESAGRAAPLMDSDSAGLVLIVDDVPDNLAVLHDALDESGYTVLVATGG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCEEEEECCC
ETALARAAQAMPDVVLLDAMMPGMDGFEVARRLKARPATAHIPIIFMTGLTETEHLEAAL
HHHHHHHHHHCCCCEEHHHHCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHH
AAGGVDYVTKPIKPREVLARMNVHLQGARRARQDMRHAGQARNALDAFGYASITVRASDG
HHCCHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCC
RLMWQTALARTLLREYFPAEGDGPRDPAATPQPVAEWLRALLPQIPVFAAQRTEPPRLVV
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHH
ERGARRLSFSLHQQTGDGEGGGDWLVVMREVSDAAVIEALALGFRLTAREAEVLYWLLKG
HHCCHHHHHEEHHCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHEECCHHHHEEEEECC
KTNRDIGDILGSSPATVKKHLERVYVKLGAETRTAAAGIAMARIRQTHPQFEG
CCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TDVPSPSAPHAESAGRAAPLMDSDSAGLVLIVDDVPDNLAVLHDALDESGYTVLVATGG
CCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCEEEEECCC
ETALARAAQAMPDVVLLDAMMPGMDGFEVARRLKARPATAHIPIIFMTGLTETEHLEAAL
HHHHHHHHHHCCCCEEHHHHCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHH
AAGGVDYVTKPIKPREVLARMNVHLQGARRARQDMRHAGQARNALDAFGYASITVRASDG
HHCCHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCC
RLMWQTALARTLLREYFPAEGDGPRDPAATPQPVAEWLRALLPQIPVFAAQRTEPPRLVV
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHH
ERGARRLSFSLHQQTGDGEGGGDWLVVMREVSDAAVIEALALGFRLTAREAEVLYWLLKG
HHCCHHHHHEEHHCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHEECCHHHHEEEEECC
KTNRDIGDILGSSPATVKKHLERVYVKLGAETRTAAAGIAMARIRQTHPQFEG
CCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA