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The map label for this gene is phaF [H]

Identifier: 120611949

GI number: 120611949

Start: 3642085

End: 3642375

Strand: Direct

Name: phaF [H]

Synonym: Aave_3293

Alternate gene names: 120611949

Gene position: 3642085-3642375 (Clockwise)

Preceding gene: 120611948

Following gene: 120611950

Centisome position: 68.04

GC content: 65.64

Gene sequence:

>291_bases
ATGACGACGACCACCAGCCCCGTGCTTTCCTGGGCCCTGCCCATCGCGCTGGCCATGCTCGCGCTGGGCATCGTGTTCGC
GCTGGTGCGCATCCTCAAGGGCCCTTCCGCCCAGGACCGCGTACTGGCGCTGGACTGCATGTACCAGAGCGGCATGCTGG
TCATGCTGGTGCTGGGCATCTACCACGGCAGCACCAACTACTTCGAGGCGGCGCTGCTCATCGCCCTGCTGGGGTTCGCG
AGTTCCACCGCGATGGCGAAATTCCTGCTGCGCGGCGAGGTGATCGAATGA

Upstream 100 bases:

>100_bases
CCGTGCTGATGCTGCACGTGCTGGAGCTCGACAGCCACGAGGCCGTGATCGGCCACATCAAGCAGCGCTACGAACGCCCC
CTGATGGAGATCTTCGAATC

Downstream 100 bases:

>100_bases
CGGCCGCCCTGCCGCTGTGGGCCGAGATCGCCGTGGCCGTGCTGGTCGTGGCGGGCGCGGCCATCGCGATGCTGGGCTCG
CTGGGCCTGCTACGGCTCAA

Product: multiple resistance and pH regulation protein F

Products: NA

Alternate protein names: pH adaptation potassium efflux system protein F; Pha system subunit F [H]

Number of amino acids: Translated: 96; Mature: 95

Protein sequence:

>96_residues
MTTTTSPVLSWALPIALAMLALGIVFALVRILKGPSAQDRVLALDCMYQSGMLVMLVLGIYHGSTNYFEAALLIALLGFA
SSTAMAKFLLRGEVIE

Sequences:

>Translated_96_residues
MTTTTSPVLSWALPIALAMLALGIVFALVRILKGPSAQDRVLALDCMYQSGMLVMLVLGIYHGSTNYFEAALLIALLGFA
SSTAMAKFLLRGEVIE
>Mature_95_residues
TTTTSPVLSWALPIALAMLALGIVFALVRILKGPSAQDRVLALDCMYQSGMLVMLVLGIYHGSTNYFEAALLIALLGFAS
STAMAKFLLRGEVIE

Specific function: Part of a K(+) efflux system which is required for the adaptation of R.meliloti to alkaline pH as well as for the infection process during symbiotic nodule development [H]

COG id: COG2212

COG function: function code P; Multisubunit Na+/H+ antiporter, MnhF subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit F family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007208 [H]

Pfam domain/function: PF04066 MrpF_PhaF [H]

EC number: NA

Molecular weight: Translated: 10264; Mature: 10132

Theoretical pI: Translated: 7.35; Mature: 7.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
6.2 %Met     (Translated Protein)
7.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTTSPVLSWALPIALAMLALGIVFALVRILKGPSAQDRVLALDCMYQSGMLVMLVLGI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHH
YHGSTNYFEAALLIALLGFASSTAMAKFLLRGEVIE
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TTTTSPVLSWALPIALAMLALGIVFALVRILKGPSAQDRVLALDCMYQSGMLVMLVLGI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHH
YHGSTNYFEAALLIALLGFASSTAMAKFLLRGEVIE
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9680201; 11481430 [H]