Click here to switch to the map view.

The map label for this gene is cysU [H]

Identifier: 120611215

GI number: 120611215

Start: 2795135

End: 2796010

Strand: Direct

Name: cysU [H]

Synonym: Aave_2546

Alternate gene names: 120611215

Gene position: 2795135-2796010 (Clockwise)

Preceding gene: 120611214

Following gene: 120611216

Centisome position: 52.22

GC content: 67.81

Gene sequence:

>876_bases
ATGGGCGCGGCCATCCCCTCCCCGGGCGCCCGGGCGCCCGCGACCGGCCGTGCCCGCAGCGGCGGCTCCCGCCGCGTGCT
GCCCGGCTTCGGACTCACGCTGGGCTACACGCTCTTCTACCTGAGCATCATCGTGCTCATCCCGCTGTCGGCGCTGCTGT
TCAAGACGTTCACGCTCACCTGGGACCAGTTCTGGTCGGCCATCAGCGCGCCGCGCGTGCTGGCTTCGTACCGGCTCACT
TTCGGCGCGTCGTTCCTGGCCGCGCTGGTCAATCTCGTGTTCGGCCTGCTGATCGCCTGGGTGCTGGTGCGCTACCGCTT
TCCCGGCAAGAAGATCGTCGATGCGCTGGTCGATCTGCCGTTCGCGCTGCCGACCGCCGTGGCCGGCATCTCGCTCACCG
CGCTGCTCGCGGGCAACGGCTGGGTGGGGCAGTACCTGGAGCCCCTGGGCATCCAGCTCGCGTTCAAGCCCGCGGGCGTG
GTCATCGCCCTGATCTTCATCGGGCTGCCGTTCGTGGTCCGCACCGTGCAGCCCGTCCTGGAGGACGCCGAGAAGGAACT
GGAGGAAGCCGCCACCAGCCTGGGCGCGACCCGCTGGCAGATCTTCACCAAGGTCATCCTGCCTTCCATCACGCCCGCGC
TGCTCACGGGCTTCGCCATGGCCTTCGCGCGCGCGGTGGGTGAATACGGGTCGGTGATCTTCATCGCCGGCAACATGCCC
ATGGTGTCCGAGATCACGCCCCTCATCATCATCGGCAAGCTGGAGCAGTACGACTACGCGGGCGCCACGGCGGTGGCCGT
GGTGATGCTGGTCATTTCCTTCGCGCTGCTTTTCATCATCAACGCGCTGCAGGCCTGGCAGCGCAAGAGCACATGA

Upstream 100 bases:

>100_bases
CGTCGCCAAGTATTTCGGATCGCTCGGCGAGGCGCAGAAGGTGCACTTCAACGACGGCGGCCAGTTCGACAAGCTCTACA
CCCCCGGCGGTCGCTGATCC

Downstream 100 bases:

>100_bases
TGGCCCGGACGTGCATATGAGCAACCACCCGACACGCACCCTCCGCCGTGCCCAGGCAGGCACCACCGAAGCCCCCTGGG
TCCGCTGGACGCTGATCGGC

Product: sulfate ABC transporter inner membrane subunit CysT

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MGAAIPSPGARAPATGRARSGGSRRVLPGFGLTLGYTLFYLSIIVLIPLSALLFKTFTLTWDQFWSAISAPRVLASYRLT
FGASFLAALVNLVFGLLIAWVLVRYRFPGKKIVDALVDLPFALPTAVAGISLTALLAGNGWVGQYLEPLGIQLAFKPAGV
VIALIFIGLPFVVRTVQPVLEDAEKELEEAATSLGATRWQIFTKVILPSITPALLTGFAMAFARAVGEYGSVIFIAGNMP
MVSEITPLIIIGKLEQYDYAGATAVAVVMLVISFALLFIINALQAWQRKST

Sequences:

>Translated_291_residues
MGAAIPSPGARAPATGRARSGGSRRVLPGFGLTLGYTLFYLSIIVLIPLSALLFKTFTLTWDQFWSAISAPRVLASYRLT
FGASFLAALVNLVFGLLIAWVLVRYRFPGKKIVDALVDLPFALPTAVAGISLTALLAGNGWVGQYLEPLGIQLAFKPAGV
VIALIFIGLPFVVRTVQPVLEDAEKELEEAATSLGATRWQIFTKVILPSITPALLTGFAMAFARAVGEYGSVIFIAGNMP
MVSEITPLIIIGKLEQYDYAGATAVAVVMLVISFALLFIINALQAWQRKST
>Mature_290_residues
GAAIPSPGARAPATGRARSGGSRRVLPGFGLTLGYTLFYLSIIVLIPLSALLFKTFTLTWDQFWSAISAPRVLASYRLTF
GASFLAALVNLVFGLLIAWVLVRYRFPGKKIVDALVDLPFALPTAVAGISLTALLAGNGWVGQYLEPLGIQLAFKPAGVV
IALIFIGLPFVVRTVQPVLEDAEKELEEAATSLGATRWQIFTKVILPSITPALLTGFAMAFARAVGEYGSVIFIAGNMPM
VSEITPLIIIGKLEQYDYAGATAVAVVMLVISFALLFIINALQAWQRKST

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788764, Length=264, Percent_Identity=51.5151515151515, Blast_Score=257, Evalue=8e-70,
Organism=Escherichia coli, GI87082099, Length=257, Percent_Identity=32.6848249027237, Blast_Score=140, Evalue=8e-35,
Organism=Escherichia coli, GI1786980, Length=209, Percent_Identity=34.9282296650718, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1786254, Length=209, Percent_Identity=26.3157894736842, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787369, Length=155, Percent_Identity=29.0322580645161, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1787368, Length=221, Percent_Identity=31.6742081447964, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011865
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31135; Mature: 31004

Theoretical pI: Translated: 10.19; Mature: 10.19

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGAAIPSPGARAPATGRARSGGSRRVLPGFGLTLGYTLFYLSIIVLIPLSALLFKTFTLT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WDQFWSAISAPRVLASYRLTFGASFLAALVNLVFGLLIAWVLVRYRFPGKKIVDALVDLP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCC
FALPTAVAGISLTALLAGNGWVGQYLEPLGIQLAFKPAGVVIALIFIGLPFVVRTVQPVL
HHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHH
EDAEKELEEAATSLGATRWQIFTKVILPSITPALLTGFAMAFARAVGEYGSVIFIAGNMP
HHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCC
MVSEITPLIIIGKLEQYDYAGATAVAVVMLVISFALLFIINALQAWQRKST
CHHHCCCEEEEECHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GAAIPSPGARAPATGRARSGGSRRVLPGFGLTLGYTLFYLSIIVLIPLSALLFKTFTLT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WDQFWSAISAPRVLASYRLTFGASFLAALVNLVFGLLIAWVLVRYRFPGKKIVDALVDLP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCC
FALPTAVAGISLTALLAGNGWVGQYLEPLGIQLAFKPAGVVIALIFIGLPFVVRTVQPVL
HHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHH
EDAEKELEEAATSLGATRWQIFTKVILPSITPALLTGFAMAFARAVGEYGSVIFIAGNMP
HHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCC
MVSEITPLIIIGKLEQYDYAGATAVAVVMLVISFALLFIINALQAWQRKST
CHHHCCCEEEEECHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2188958; 9205837; 9278503; 2188959 [H]