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The map label for this gene is oprM [H]

Identifier: 120611198

GI number: 120611198

Start: 2776539

End: 2777996

Strand: Direct

Name: oprM [H]

Synonym: Aave_2529

Alternate gene names: 120611198

Gene position: 2776539-2777996 (Clockwise)

Preceding gene: 120611197

Following gene: 120611200

Centisome position: 51.87

GC content: 75.03

Gene sequence:

>1458_bases
ATGCGTACCGTTGCGATGATGCCTCTCCTGGCGCTGCTGTCCGCCTGCACGCTGACCCCTCCGCTGGAACGGCCGGCTCC
TCCGGTGCCCGCGGTGTTCCCGGACTACGGCGGCCGTGCTGGCGCCGCTGCCTCGCTCTCGCCAGGCACTTCCGTGCGTC
CGGACGTGCCCGTGGCCGACCTGGGCTGGCGCGACATGTTCGAAGATCCGCGCCTGCAGCGGCTGATCGCGGCCGCGCTG
GAGCACAACCGCGACCTGCGGCTGGCGGCGCTGCAGGCCGAAGCCGTGCAGGCCCAGTACCGCATCCAGCGCTCCGCGCG
CTGGCCCGAGGTGGCGCTGGACGCCTCCGCGTCGCGCGAGCGCGGGGCCCGCTCCGGCACCGGAGCCGGTGCGGCCGGGT
CCGCTGGCGCGGCGGTTTCCGGGCAGGCGGCCTTGGGCGTGGGCGTGAGCGCGTTCGAACTGGACCTGTTCGGCCGCGTC
CGGTCGCTGTCCGATGCCGCGCTGGCGCGTTACCTGGCCAGCGAGCACGGCCACCGCGCCGCACAGATCACCCTGGTGGG
CGCGGTGGCGGACGCGTATTTCGCCCAGCGGCTGGCGCAGGAACAGCTGCAGCTCACCGTGCGCACGCTTTCGGATTGGC
GGCAATCCCTGGAACTGGCGCGGCTATGGCGGCAGGCCGGGCAGAACAGCGGGCTGGACGTGGTGCAGGCCGAAGGGCAG
GTCGCCGCCGCGGAGGCCGACCTGGAGGCTCGCCAGCGGGCGCTCGACCAGGCGGACAACGCCCTGCGGCTGCTGCTGGG
CTCCGATCCGCCGGCAGTCCTGCCCGAGCCGCTGCCGCTGGAGGCGCGGCCCGTCGCCGCCCGTCTGCCGGCGGGGCTGC
CCTCCGGCCTGCTGCTGGCGCGGCCGGACCTGCTGCAGGCCGAACAGGCGCTCGTCGCCGCGCATGCCGATGTCGGCGCG
GCGCGGGCGGCGTTCTTTCCGCGGATCGCGCTCACCGCGTCCGTCGGCCGTGCCAGCGATTCGCTGGGCGGCCTGTTCGA
CGCGGGCTGGGGCACCTGGCGCTTCGCGCCGCAGATCACCCAGCCGCTCTTCGACCGCGGTCGCCTGCGCGGGGAACTGC
GCCTCGCCCAAGTGCGGCGCTCCGAGGCTGTGGTCCAGTACGAACGGGCGATCCAGGTCGCCTTCCGCGAGGTGGCCGAT
GCGCTCGCGGGAGCGGCCACCTACGGCCGGCAGATCGACGCCCAGGCGCGTACCGTGGCCAGCGCCGCCCGGCGCGTGGA
GCTGTCGGAACTGCGCTACCGGGCGGGCACGGACGGGCGACTGGAACTGCTGGACGCCCGGCGCCAGCAGTACGCCGCGC
AGCAGGCGCTGCTGGACCTGCGCCGCGCGGAGCTCGGCAATGCGGTGGCGCTCTACAAGGCGCTGGGTGGCGGGCTGGTC
GAGCGGGCCCATCGTTGA

Upstream 100 bases:

>100_bases
GGAGCGGGTGGTCGCATGGCGGGCCCGCCGCCGCCCGCCAGAGGCGCTTCCGGCACCGGCCGGCGCACCCGCTGTACCCA
CCACGGGAGGGCAGTGAGCC

Downstream 100 bases:

>100_bases
TTGTTGCAAGCATGACAAGGCCGCGACAGCGCACATTTGTCCCAAACTCGCTATGCGCAGCCCGCTTCGTTTTGTTGATT
GGAAATTCGCTTGATTCGAT

Product: RND efflux system outer membrane lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MRTVAMMPLLALLSACTLTPPLERPAPPVPAVFPDYGGRAGAAASLSPGTSVRPDVPVADLGWRDMFEDPRLQRLIAAAL
EHNRDLRLAALQAEAVQAQYRIQRSARWPEVALDASASRERGARSGTGAGAAGSAGAAVSGQAALGVGVSAFELDLFGRV
RSLSDAALARYLASEHGHRAAQITLVGAVADAYFAQRLAQEQLQLTVRTLSDWRQSLELARLWRQAGQNSGLDVVQAEGQ
VAAAEADLEARQRALDQADNALRLLLGSDPPAVLPEPLPLEARPVAARLPAGLPSGLLLARPDLLQAEQALVAAHADVGA
ARAAFFPRIALTASVGRASDSLGGLFDAGWGTWRFAPQITQPLFDRGRLRGELRLAQVRRSEAVVQYERAIQVAFREVAD
ALAGAATYGRQIDAQARTVASAARRVELSELRYRAGTDGRLELLDARRQQYAAQQALLDLRRAELGNAVALYKALGGGLV
ERAHR

Sequences:

>Translated_485_residues
MRTVAMMPLLALLSACTLTPPLERPAPPVPAVFPDYGGRAGAAASLSPGTSVRPDVPVADLGWRDMFEDPRLQRLIAAAL
EHNRDLRLAALQAEAVQAQYRIQRSARWPEVALDASASRERGARSGTGAGAAGSAGAAVSGQAALGVGVSAFELDLFGRV
RSLSDAALARYLASEHGHRAAQITLVGAVADAYFAQRLAQEQLQLTVRTLSDWRQSLELARLWRQAGQNSGLDVVQAEGQ
VAAAEADLEARQRALDQADNALRLLLGSDPPAVLPEPLPLEARPVAARLPAGLPSGLLLARPDLLQAEQALVAAHADVGA
ARAAFFPRIALTASVGRASDSLGGLFDAGWGTWRFAPQITQPLFDRGRLRGELRLAQVRRSEAVVQYERAIQVAFREVAD
ALAGAATYGRQIDAQARTVASAARRVELSELRYRAGTDGRLELLDARRQQYAAQQALLDLRRAELGNAVALYKALGGGLV
ERAHR
>Mature_485_residues
MRTVAMMPLLALLSACTLTPPLERPAPPVPAVFPDYGGRAGAAASLSPGTSVRPDVPVADLGWRDMFEDPRLQRLIAAAL
EHNRDLRLAALQAEAVQAQYRIQRSARWPEVALDASASRERGARSGTGAGAAGSAGAAVSGQAALGVGVSAFELDLFGRV
RSLSDAALARYLASEHGHRAAQITLVGAVADAYFAQRLAQEQLQLTVRTLSDWRQSLELARLWRQAGQNSGLDVVQAEGQ
VAAAEADLEARQRALDQADNALRLLLGSDPPAVLPEPLPLEARPVAARLPAGLPSGLLLARPDLLQAEQALVAAHADVGA
ARAAFFPRIALTASVGRASDSLGGLFDAGWGTWRFAPQITQPLFDRGRLRGELRLAQVRRSEAVVQYERAIQVAFREVAD
ALAGAATYGRQIDAQARTVASAARRVELSELRYRAGTDGRLELLDARRQQYAAQQALLDLRRAELGNAVALYKALGGGLV
ERAHR

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=481, Percent_Identity=40.3326403326403, Blast_Score=325, Evalue=5e-90,
Organism=Escherichia coli, GI2367348, Length=430, Percent_Identity=28.3720930232558, Blast_Score=100, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 51661; Mature: 51661

Theoretical pI: Translated: 9.78; Mature: 9.78

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTVAMMPLLALLSACTLTPPLERPAPPVPAVFPDYGGRAGAAASLSPGTSVRPDVPVAD
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
LGWRDMFEDPRLQRLIAAALEHNRDLRLAALQAEAVQAQYRIQRSARWPEVALDASASRE
CCCHHHCCCHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHH
RGARSGTGAGAAGSAGAAVSGQAALGVGVSAFELDLFGRVRSLSDAALARYLASEHGHRA
CCCCCCCCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
AQITLVGAVADAYFAQRLAQEQLQLTVRTLSDWRQSLELARLWRQAGQNSGLDVVQAEGQ
EEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCC
VAAAEADLEARQRALDQADNALRLLLGSDPPAVLPEPLPLEARPVAARLPAGLPSGLLLA
EEEHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCEEE
RPDLLQAEQALVAAHADVGAARAAFFPRIALTASVGRASDSLGGLFDAGWGTWRFAPQIT
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHCCHHCCCCCCEEECHHHH
QPLFDRGRLRGELRLAQVRRSEAVVQYERAIQVAFREVADALAGAATYGRQIDAQARTVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
SAARRVELSELRYRAGTDGRLELLDARRQQYAAQQALLDLRRAELGNAVALYKALGGGLV
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHH
ERAHR
HHCCC
>Mature Secondary Structure
MRTVAMMPLLALLSACTLTPPLERPAPPVPAVFPDYGGRAGAAASLSPGTSVRPDVPVAD
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
LGWRDMFEDPRLQRLIAAALEHNRDLRLAALQAEAVQAQYRIQRSARWPEVALDASASRE
CCCHHHCCCHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHH
RGARSGTGAGAAGSAGAAVSGQAALGVGVSAFELDLFGRVRSLSDAALARYLASEHGHRA
CCCCCCCCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
AQITLVGAVADAYFAQRLAQEQLQLTVRTLSDWRQSLELARLWRQAGQNSGLDVVQAEGQ
EEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCC
VAAAEADLEARQRALDQADNALRLLLGSDPPAVLPEPLPLEARPVAARLPAGLPSGLLLA
EEEHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCEEE
RPDLLQAEQALVAAHADVGAARAAFFPRIALTASVGRASDSLGGLFDAGWGTWRFAPQIT
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHCCHHCCCCCCEEECHHHH
QPLFDRGRLRGELRLAQVRRSEAVVQYERAIQVAFREVADALAGAATYGRQIDAQARTVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
SAARRVELSELRYRAGTDGRLELLDARRQQYAAQQALLDLRRAELGNAVALYKALGGGLV
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHH
ERAHR
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]