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The map label for this gene is cls [H]

Identifier: 120610964

GI number: 120610964

Start: 2520401

End: 2521684

Strand: Direct

Name: cls [H]

Synonym: Aave_2290

Alternate gene names: 120610964

Gene position: 2520401-2521684 (Clockwise)

Preceding gene: 120610963

Following gene: 120610967

Centisome position: 47.09

GC content: 68.54

Gene sequence:

>1284_bases
ATGCAGACACCCGGCCGTACGCTGCGCACAGCGCTGATCACCTTCATCGCGACCGCCGCCGCGGCGTTGCTGGTACTCAA
TTTCACCGCGGGCGAGCCGCAGGTCCGCCAGCAGCTGCCGCGCCTCTACGGCACCTCCAGCCCCCAGTTCGAGCGGGCCA
TGGGCAACCTGCTGGGGCCTTCCATCGTGGAGGGCAACGACGTGATGGACCTGCAGAACGGCGACCGCATCTTCCCGCCC
ATGCTCGCCGCCATCCGCGCCGCACGCCGTTCCATCACCTTCGAGACCTACATCTACTGGTCCGGCGACATCGGCAAGCA
GTTCGCCGACGCCCTGAGCGAACGCGCCCGCGCGGGCGTCAAGGTGCATGTGCTGCTCGACTGGGTGGGCAGCTCCAAGA
TGGAGGACAGCTACCTCGACGAGATGCGCGCCGCCGGCGTGGAGATCGAGAAATTCCACAAGCCCCACTGGTACAACCTC
GCGCGGCTCAACAACCGCACCCACCGCAAGCTGCTCGTGGCGGACGGCGTGGTGGGCTTTACCGGCGGCGTGGGCATCGC
GCCCGAGTGGACCGGTAACGCGCAGGACCCGGACCACTGGCGCGACTCGCACTACCTCGTGCGCGGCCCGGCGGTGGCGC
ACCTGCAGGCCACCTTCCTGGACAACTGGCTCAAGGTCACCGGCCGGGTGCTGCACGGCGACGCCTATTTCCCGAAGCTC
GCGCCCGCCGGCACGCAGCGCGCGCAGATGTTCTCCAGCTCGCCATCGGGCGGCAGCGAGAGCATGCAGCTCATGTACCA
CATGGCCGTCACCGCGGCCGAGCGCAGCATCGACCTTTCCGCGGCGTATTTCGTGCCCGATGAACTCACCCGCGCCCTGC
TGCTGGACGCGCTGCGCCGCGGCGTGCGCGTGCGCATCGTCACCCCGGGCGAGCACACCGACACCGAAACCGTCAAGTCC
GCCTCGCGCGGCACCTGGGGCCCGCTGCTGGAGGCGGGCGCGGAGATCTACACCTACGGCCCCACCATGTACCACTGCAA
GGTCATGATCGTGGACCAGCGCCTCGTCTCGGTCGGCTCCACCAACTTCGACAACCGCTCCTTCCGCCTCAACGACGAAG
CCAACCTCAACGTCTATGACGAGGCCTTCGCCCGGCGCCAGACCGAGGTCTTCGAGCAGGACATCCGCCAGTCCCGCCGG
GTCACGCACCAGGAATGGCTGAACCGGCCCCTGCGGGAGCGGCTCGCCGAGCGCATCACCGGCCTGCTCGAGTCGCAACT
CTGA

Upstream 100 bases:

>100_bases
CGATGGCGCGCGGCGCCCGTCCGGGTAGGCCCGCCAGTGTAGGCGGCTGGAGGCGCCGGCCTACAGGGGCGGCCCGCGGC
GGCTTCGACAATCCCCTGCC

Downstream 100 bases:

>100_bases
ATCAGCCAGGAATCTCCGGCTCCACCAGCAGCGCCAGCAACTGCAGCGACTCCTGCCACCCGAGATAGCAGGCCTCGGTG
GGAATCATCGCGGGAATGCC

Product: phospholipase D/transphosphatidylase

Products: Biphosphatidylglycerol; Glycerol. [C]

Alternate protein names: CL synthase [H]

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MQTPGRTLRTALITFIATAAAALLVLNFTAGEPQVRQQLPRLYGTSSPQFERAMGNLLGPSIVEGNDVMDLQNGDRIFPP
MLAAIRAARRSITFETYIYWSGDIGKQFADALSERARAGVKVHVLLDWVGSSKMEDSYLDEMRAAGVEIEKFHKPHWYNL
ARLNNRTHRKLLVADGVVGFTGGVGIAPEWTGNAQDPDHWRDSHYLVRGPAVAHLQATFLDNWLKVTGRVLHGDAYFPKL
APAGTQRAQMFSSSPSGGSESMQLMYHMAVTAAERSIDLSAAYFVPDELTRALLLDALRRGVRVRIVTPGEHTDTETVKS
ASRGTWGPLLEAGAEIYTYGPTMYHCKVMIVDQRLVSVGSTNFDNRSFRLNDEANLNVYDEAFARRQTEVFEQDIRQSRR
VTHQEWLNRPLRERLAERITGLLESQL

Sequences:

>Translated_427_residues
MQTPGRTLRTALITFIATAAAALLVLNFTAGEPQVRQQLPRLYGTSSPQFERAMGNLLGPSIVEGNDVMDLQNGDRIFPP
MLAAIRAARRSITFETYIYWSGDIGKQFADALSERARAGVKVHVLLDWVGSSKMEDSYLDEMRAAGVEIEKFHKPHWYNL
ARLNNRTHRKLLVADGVVGFTGGVGIAPEWTGNAQDPDHWRDSHYLVRGPAVAHLQATFLDNWLKVTGRVLHGDAYFPKL
APAGTQRAQMFSSSPSGGSESMQLMYHMAVTAAERSIDLSAAYFVPDELTRALLLDALRRGVRVRIVTPGEHTDTETVKS
ASRGTWGPLLEAGAEIYTYGPTMYHCKVMIVDQRLVSVGSTNFDNRSFRLNDEANLNVYDEAFARRQTEVFEQDIRQSRR
VTHQEWLNRPLRERLAERITGLLESQL
>Mature_427_residues
MQTPGRTLRTALITFIATAAAALLVLNFTAGEPQVRQQLPRLYGTSSPQFERAMGNLLGPSIVEGNDVMDLQNGDRIFPP
MLAAIRAARRSITFETYIYWSGDIGKQFADALSERARAGVKVHVLLDWVGSSKMEDSYLDEMRAAGVEIEKFHKPHWYNL
ARLNNRTHRKLLVADGVVGFTGGVGIAPEWTGNAQDPDHWRDSHYLVRGPAVAHLQATFLDNWLKVTGRVLHGDAYFPKL
APAGTQRAQMFSSSPSGGSESMQLMYHMAVTAAERSIDLSAAYFVPDELTRALLLDALRRGVRVRIVTPGEHTDTETVKS
ASRGTWGPLLEAGAEIYTYGPTMYHCKVMIVDQRLVSVGSTNFDNRSFRLNDEANLNVYDEAFARRQTEVFEQDIRQSRR
VTHQEWLNRPLRERLAERITGLLESQL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=369, Percent_Identity=29.5392953929539, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI1787007, Length=324, Percent_Identity=29.6296296296296, Blast_Score=137, Evalue=9e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.- [C]

Molecular weight: Translated: 47803; Mature: 47803

Theoretical pI: Translated: 7.90; Mature: 7.90

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTPGRTLRTALITFIATAAAALLVLNFTAGEPQVRQQLPRLYGTSSPQFERAMGNLLGP
CCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCC
SIVEGNDVMDLQNGDRIFPPMLAAIRAARRSITFETYIYWSGDIGKQFADALSERARAGV
CEECCCCEEECCCCCEECHHHHHHHHHHHHCEEEEEEEEECCCHHHHHHHHHHHHHHCCC
KVHVLLDWVGSSKMEDSYLDEMRAAGVEIEKFHKPHWYNLARLNNRTHRKLLVADGVVGF
EEEEEEEECCCCCCCHHHHHHHHHCCCCHHHCCCCCCEEHHHHCCCCHHEEEEECCCEEC
TGGVGIAPEWTGNAQDPDHWRDSHYLVRGPAVAHLQATFLDNWLKVTGRVLHGDAYFPKL
CCCCCCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHCEEEECCCCCCCC
APAGTQRAQMFSSSPSGGSESMQLMYHMAVTAAERSIDLSAAYFVPDELTRALLLDALRR
CCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHC
GVRVRIVTPGEHTDTETVKSASRGTWGPLLEAGAEIYTYGPTMYHCKVMIVDQRLVSVGS
CCEEEEECCCCCCCHHHHHHCCCCCCHHHHHCCCEEEEECCCEEEEEEEEEEHHHHHHCC
TNFDNRSFRLNDEANLNVYDEAFARRQTEVFEQDIRQSRRVTHQEWLNRPLRERLAERIT
CCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHH
GLLESQL
HHHHHCC
>Mature Secondary Structure
MQTPGRTLRTALITFIATAAAALLVLNFTAGEPQVRQQLPRLYGTSSPQFERAMGNLLGP
CCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCC
SIVEGNDVMDLQNGDRIFPPMLAAIRAARRSITFETYIYWSGDIGKQFADALSERARAGV
CEECCCCEEECCCCCEECHHHHHHHHHHHHCEEEEEEEEECCCHHHHHHHHHHHHHHCCC
KVHVLLDWVGSSKMEDSYLDEMRAAGVEIEKFHKPHWYNLARLNNRTHRKLLVADGVVGF
EEEEEEEECCCCCCCHHHHHHHHHCCCCHHHCCCCCCEEHHHHCCCCHHEEEEECCCEEC
TGGVGIAPEWTGNAQDPDHWRDSHYLVRGPAVAHLQATFLDNWLKVTGRVLHGDAYFPKL
CCCCCCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHCEEEECCCCCCCC
APAGTQRAQMFSSSPSGGSESMQLMYHMAVTAAERSIDLSAAYFVPDELTRALLLDALRR
CCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHC
GVRVRIVTPGEHTDTETVKSASRGTWGPLLEAGAEIYTYGPTMYHCKVMIVDQRLVSVGS
CCEEEEECCCCCCCHHHHHHCCCCCCHHHHHCCCEEEEECCCEEEEEEEEEEHHHHHHCC
TNFDNRSFRLNDEANLNVYDEAFARRQTEVFEQDIRQSRRVTHQEWLNRPLRERLAERIT
CCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHH
GLLESQL
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Phosphatidylglycerol [C]

Specific reaction: 2 Phosphatidylglycerol = Biphosphatidylglycerol + Glycerol. [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA