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The map label for this gene is mdh

Identifier: 120610880

GI number: 120610880

Start: 2425711

End: 2426697

Strand: Direct

Name: mdh

Synonym: Aave_2206

Alternate gene names: 120610880

Gene position: 2425711-2426697 (Clockwise)

Preceding gene: 120610872

Following gene: 120610881

Centisome position: 45.32

GC content: 65.86

Gene sequence:

>987_bases
ATGAGCAAGAAGCCTGTCCGCGTCGCCGTCACCGGTGCAGCCGGTCAAATTGGTTACGCACTGCTGTTCCGCATCGCCTC
CGGCGAAATGCTCGGCAAGGACCAGCCCGTCATCCTGCAACTGCTGGAAATTCCCGACGAGAAGGCCCAGAAGGCGCTCA
AGGGCGTGATGATGGAACTGGAAGACTGCGCGTTCCCGCTGCTGGCCGGCATGGAAGCCCACAGCGACCCGATGACCGCC
TTCAAGGACACCGACTACGCCCTGCTGGTGGGCGCACGTCCGCGCGGCCCCGGCATGGAGCGCGCCGACCTGCTGGCCGC
CAATGCCCAGATCTTCACGGCCCAGGGCAAGGCCCTGAACGCCGTCGCCTCGCGCAACGTGAAGGTTCTCGTGGTGGGCA
ACCCCGCCAACACCAACGCCTACATCGCGATGAAGTCGGCCCCCGACCTGCCGGCCAAGAACTTCACCGCCATGCTGCGC
CTGGACCACAATCGCGCCGCCAGCCAGATCGCCGCCAAGACGGGCGGCAAGGTCGGCGAGATCGAGAAGCTGACGGTGTG
GGGCAACCACTCGCCCACGATGTACGCCGATTACCGTTTCGCCACCATCGGCGGCAAGAGCGTCAAGGACGCCATCAACG
ACCAGGTCTGGAACGCCGACGTGTTCCTGCCCACGGTGGGCAAGCGCGGCGCCGCCATCATCGAGGCGCGCGGCCTGTCC
TCCGCCGCCTCGGCCGCCAACGCCGCCATCGACCACATGCGCGACTGGGCCCTGGGCTCGAACGGCAAGTGGGTCACTAT
GGGCGTGCCCTCCAAGGGCGAGTACGGCATTCCCGAAGGCATCGTGTTCGGCTTCCCGGTGATCACCGAGAACGGCGAAT
ACAAGATCGTCGAGGGCCTGGAGATCGACGCGTTCAGCCAGGAGCGCATCAACAAGACGCTGGCCGAGCTGCAAGGCGAA
CAGGACGGCGTGAAGCACCTGCTGTAA

Upstream 100 bases:

>100_bases
CACTCGGGAGCTGTTCTGCAGGACCCTGGCTGCTTGGATCGACTGGCGCTGGTGCCCCTGCACCGACACCCGTTCCATCC
CATCTTCTGGAGTTTCCCTC

Downstream 100 bases:

>100_bases
GGCGGGCCTGCCCGACGGCTGCACCTGTACCGCGACGCCATGCACGACTGGAACCCCGCGCTCTACCGACGCTTCGAGGA
CGAGCGCACGCGTCCGGCCC

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 327

Protein sequence:

>328_residues
MSKKPVRVAVTGAAGQIGYALLFRIASGEMLGKDQPVILQLLEIPDEKAQKALKGVMMELEDCAFPLLAGMEAHSDPMTA
FKDTDYALLVGARPRGPGMERADLLAANAQIFTAQGKALNAVASRNVKVLVVGNPANTNAYIAMKSAPDLPAKNFTAMLR
LDHNRAASQIAAKTGGKVGEIEKLTVWGNHSPTMYADYRFATIGGKSVKDAINDQVWNADVFLPTVGKRGAAIIEARGLS
SAASAANAAIDHMRDWALGSNGKWVTMGVPSKGEYGIPEGIVFGFPVITENGEYKIVEGLEIDAFSQERINKTLAELQGE
QDGVKHLL

Sequences:

>Translated_328_residues
MSKKPVRVAVTGAAGQIGYALLFRIASGEMLGKDQPVILQLLEIPDEKAQKALKGVMMELEDCAFPLLAGMEAHSDPMTA
FKDTDYALLVGARPRGPGMERADLLAANAQIFTAQGKALNAVASRNVKVLVVGNPANTNAYIAMKSAPDLPAKNFTAMLR
LDHNRAASQIAAKTGGKVGEIEKLTVWGNHSPTMYADYRFATIGGKSVKDAINDQVWNADVFLPTVGKRGAAIIEARGLS
SAASAANAAIDHMRDWALGSNGKWVTMGVPSKGEYGIPEGIVFGFPVITENGEYKIVEGLEIDAFSQERINKTLAELQGE
QDGVKHLL
>Mature_327_residues
SKKPVRVAVTGAAGQIGYALLFRIASGEMLGKDQPVILQLLEIPDEKAQKALKGVMMELEDCAFPLLAGMEAHSDPMTAF
KDTDYALLVGARPRGPGMERADLLAANAQIFTAQGKALNAVASRNVKVLVVGNPANTNAYIAMKSAPDLPAKNFTAMLRL
DHNRAASQIAAKTGGKVGEIEKLTVWGNHSPTMYADYRFATIGGKSVKDAINDQVWNADVFLPTVGKRGAAIIEARGLSS
AASAANAAIDHMRDWALGSNGKWVTMGVPSKGEYGIPEGIVFGFPVITENGEYKIVEGLEIDAFSQERINKTLAELQGEQ
DGVKHLL

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 2 family

Homologues:

Organism=Homo sapiens, GI5174539, Length=331, Percent_Identity=49.5468277945619, Blast_Score=314, Evalue=7e-86,
Organism=Homo sapiens, GI89886456, Length=337, Percent_Identity=24.9258160237389, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17561064, Length=324, Percent_Identity=52.4691358024691, Blast_Score=318, Evalue=3e-87,
Organism=Caenorhabditis elegans, GI32566798, Length=261, Percent_Identity=52.8735632183908, Blast_Score=252, Evalue=2e-67,
Organism=Caenorhabditis elegans, GI32566800, Length=170, Percent_Identity=47.6470588235294, Blast_Score=149, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24583394, Length=324, Percent_Identity=52.4691358024691, Blast_Score=313, Evalue=7e-86,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): MDH_ACIAC (A1TP96)

Other databases:

- EMBL:   CP000512
- RefSeq:   YP_970558.1
- ProteinModelPortal:   A1TP96
- SMR:   A1TP96
- STRING:   A1TP96
- GeneID:   4668190
- GenomeReviews:   CP000512_GR
- KEGG:   aav:Aave_2206
- NMPDR:   fig|397945.5.peg.1904
- eggNOG:   COG0039
- HOGENOM:   HBG289884
- OMA:   MIIWGNH
- ProtClustDB:   PRK05442
- BioCyc:   AAVE397945:AAVE_2206-MONOMER
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_01517
- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR010945
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR23382
- PIRSF:   PIRSF000102
- TIGRFAMs:   TIGR01759

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.37

Molecular weight: Translated: 34978; Mature: 34846

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: PS00068 MDH

Important sites: ACT_SITE 190-190 BINDING 95-95 BINDING 101-101 BINDING 108-108 BINDING 115-115 BINDING 134-134 BINDING 165-165

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKPVRVAVTGAAGQIGYALLFRIASGEMLGKDQPVILQLLEIPDEKAQKALKGVMMEL
CCCCCEEEEEECCCCHHHHHHHEEECCCCCCCCCCHHHHHEECCCCHHHHHHHHHHHHHH
EDCAFPLLAGMEAHSDPMTAFKDTDYALLVGARPRGPGMERADLLAANAQIFTAQGKALN
HHHHHHHHHCCCCCCCCCCEECCCCEEEEEECCCCCCCCCHHHEEEECCEEEEECCCEEE
AVASRNVKVLVVGNPANTNAYIAMKSAPDLPAKNFTAMLRLDHNRAASQIAAKTGGKVGE
HHHCCCEEEEEECCCCCCCEEEEECCCCCCCCCCEEEEEEECCCHHHHHHHHHCCCCCCC
IEKLTVWGNHSPTMYADYRFATIGGKSVKDAINDQVWNADVFLPTVGKRGAAIIEARGLS
EEEEEEECCCCCCEEEEEEEEEECCCHHHHHHCCCCCCCEEEEECCCCCCCEEEEECCCC
SAASAANAAIDHMRDWALGSNGKWVTMGVPSKGEYGIPEGIVFGFPVITENGEYKIVEGL
HHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCEEEECEEEECCCCEEEEECC
EIDAFSQERINKTLAELQGEQDGVKHLL
EECCCCHHHHHHHHHHHCCCCHHHHHCC
>Mature Secondary Structure 
SKKPVRVAVTGAAGQIGYALLFRIASGEMLGKDQPVILQLLEIPDEKAQKALKGVMMEL
CCCCEEEEEECCCCHHHHHHHEEECCCCCCCCCCHHHHHEECCCCHHHHHHHHHHHHHH
EDCAFPLLAGMEAHSDPMTAFKDTDYALLVGARPRGPGMERADLLAANAQIFTAQGKALN
HHHHHHHHHCCCCCCCCCCEECCCCEEEEEECCCCCCCCCHHHEEEECCEEEEECCCEEE
AVASRNVKVLVVGNPANTNAYIAMKSAPDLPAKNFTAMLRLDHNRAASQIAAKTGGKVGE
HHHCCCEEEEEECCCCCCCEEEEECCCCCCCCCCEEEEEEECCCHHHHHHHHHCCCCCCC
IEKLTVWGNHSPTMYADYRFATIGGKSVKDAINDQVWNADVFLPTVGKRGAAIIEARGLS
EEEEEEECCCCCCEEEEEEEEEECCCHHHHHHCCCCCCCEEEEECCCCCCCEEEEECCCC
SAASAANAAIDHMRDWALGSNGKWVTMGVPSKGEYGIPEGIVFGFPVITENGEYKIVEGL
HHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCEEEECEEEECCCCEEEEECC
EIDAFSQERINKTLAELQGEQDGVKHLL
EECCCCHHHHHHHHHHHCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA