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The map label for this gene is hipO [H]

Identifier: 120610853

GI number: 120610853

Start: 2396210

End: 2397391

Strand: Direct

Name: hipO [H]

Synonym: Aave_2179

Alternate gene names: 120610853

Gene position: 2396210-2397391 (Clockwise)

Preceding gene: 120610852

Following gene: 120610859

Centisome position: 44.77

GC content: 72.84

Gene sequence:

>1182_bases
ATGAGCCTGGAGGACGTTGCGCGGCCCGTGGCCGACCTGCTGCCCGATCTGGTCACCCTGCGCCGCGATCTGCATGCCCA
TCCCGAGCTGGCCTACCAGGAGCGCCGCACGGCCGGCATCGTGGCGCAGTCGCTGCGCCTGCTGGGCCTGGAGGTGCACG
AAGGTATCGGCGGCACGGGAGTGGTGGGCACGCTGCGGATGGGCACGGGCTCGCGCCGTGTGGGCCTGCGGGCCGACATG
GATGCGCTGCCGATGGTGGAACTGGGGCGTGCGCCCCATGCGAGCCGCCATGCGGGCGCCCACCATGGCTGCGGGCACGA
CGGGCACACGAGCATGCTGATCGGCGCGGCGCGCCAGCTCGCTCGCGCGGGTGGCCTGGACGGCACGGTGCATTTCATCT
TCCAGCCCGCGGAGGAGGGGCAGGGCGGCGCGCGCCGCATGATCGAGGACGGCCTGTTCGAGCGCTTTCCGTGCGACAGC
GTCTATGCGCTGCACAACTGGCCCGACCTGCCCCTGGGCGCTGCGCGCACGCGGCCCGGTCCCATCATGGCGGCGGCGGA
CCGCTTCGATATCGCCGTGCGCGGCCGCGGCGGCCACGCGGCGCAGCCGCACCACACGCCCGACGCCATCCTGGCGGCGA
GCCAGCTCGTCGCCCAACTCAACACCACCGTGTCGCGGCGCATCGACCCCGGCGAATCGGCCGTGCTATCCGTCACGCGC
ATCGAGGGTGGCCAGAGCCACAACGTGCTGCCCGCCGAGGTGCGCATCACCGGGACGGTGCGCAGCTTCGATCCGCACGC
GCAGGACGGGATCGAGGCGGCGCTGCGTGCCGCCTGCGAGGGCATCGCGCTGGCGAGCGGCACCGCGGTGGAGGTGGACT
ACGTGCGCTATTACCCGGCCACCATCAATACCGCTCCCGAGGCCGCGCTGGCGCTGGAGGCCGCGGCCGCCATCGGGCTG
GAGGCCGAGGTGGCGCCGCGCGCGGCGTTCACTTCGGAGGATTTCGCGTTCATGCTGCAGCGCAGACCAGGGGCGTATCT
CTGGCTGGGGCAGGGGCGCGCGGATCCGGGGCCGGAGGGGGAGAGGCCGCTGCACCATCCCTGCTATGACTTCAACGACG
AGGCGCTGGGGTTGGGGGTGCGGTGGTTCTGCGAGGTGGCGAGGCGGGCGTTGTCGGGGTGA

Upstream 100 bases:

>100_bases
GCATGGAGGGCTCCGGCGGCACCATCGAATCCGAGCACACCGTGCCGGACTACCATTTTCTGACGCTGGCGCAGCTGGCC
GACGCGGTAGAGGCGGGGCA

Downstream 100 bases:

>100_bases
TTTCTTTCCCGGGGGCCGGGATGTGCGCCCGCCGGGGCGTGGCCCCGGCCCCCCGCCCTCGAAAGAAACTCACCTCTGAT
GCGGAAACAACCTCTCGATC

Product: amidohydrolase

Products: NA

Alternate protein names: Benzoylglycine amidohydrolase; Hippuricase [H]

Number of amino acids: Translated: 393; Mature: 392

Protein sequence:

>393_residues
MSLEDVARPVADLLPDLVTLRRDLHAHPELAYQERRTAGIVAQSLRLLGLEVHEGIGGTGVVGTLRMGTGSRRVGLRADM
DALPMVELGRAPHASRHAGAHHGCGHDGHTSMLIGAARQLARAGGLDGTVHFIFQPAEEGQGGARRMIEDGLFERFPCDS
VYALHNWPDLPLGAARTRPGPIMAAADRFDIAVRGRGGHAAQPHHTPDAILAASQLVAQLNTTVSRRIDPGESAVLSVTR
IEGGQSHNVLPAEVRITGTVRSFDPHAQDGIEAALRAACEGIALASGTAVEVDYVRYYPATINTAPEAALALEAAAAIGL
EAEVAPRAAFTSEDFAFMLQRRPGAYLWLGQGRADPGPEGERPLHHPCYDFNDEALGLGVRWFCEVARRALSG

Sequences:

>Translated_393_residues
MSLEDVARPVADLLPDLVTLRRDLHAHPELAYQERRTAGIVAQSLRLLGLEVHEGIGGTGVVGTLRMGTGSRRVGLRADM
DALPMVELGRAPHASRHAGAHHGCGHDGHTSMLIGAARQLARAGGLDGTVHFIFQPAEEGQGGARRMIEDGLFERFPCDS
VYALHNWPDLPLGAARTRPGPIMAAADRFDIAVRGRGGHAAQPHHTPDAILAASQLVAQLNTTVSRRIDPGESAVLSVTR
IEGGQSHNVLPAEVRITGTVRSFDPHAQDGIEAALRAACEGIALASGTAVEVDYVRYYPATINTAPEAALALEAAAAIGL
EAEVAPRAAFTSEDFAFMLQRRPGAYLWLGQGRADPGPEGERPLHHPCYDFNDEALGLGVRWFCEVARRALSG
>Mature_392_residues
SLEDVARPVADLLPDLVTLRRDLHAHPELAYQERRTAGIVAQSLRLLGLEVHEGIGGTGVVGTLRMGTGSRRVGLRADMD
ALPMVELGRAPHASRHAGAHHGCGHDGHTSMLIGAARQLARAGGLDGTVHFIFQPAEEGQGGARRMIEDGLFERFPCDSV
YALHNWPDLPLGAARTRPGPIMAAADRFDIAVRGRGGHAAQPHHTPDAILAASQLVAQLNTTVSRRIDPGESAVLSVTRI
EGGQSHNVLPAEVRITGTVRSFDPHAQDGIEAALRAACEGIALASGTAVEVDYVRYYPATINTAPEAALALEAAAAIGLE
AEVAPRAAFTSEDFAFMLQRRPGAYLWLGQGRADPGPEGERPLHHPCYDFNDEALGLGVRWFCEVARRALSG

Specific function: Required But Not Essential For Aminobenzoyl-Glutamate Utilization. May Participate In Hydrolysis Of Aminobenzoyl- Glutamate To Aminobenzoate, Either Alone Or In Combination With Abgb. [C]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=446, Percent_Identity=28.9237668161435, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1787598, Length=321, Percent_Identity=27.4143302180685, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.32 [H]

Molecular weight: Translated: 41840; Mature: 41709

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLEDVARPVADLLPDLVTLRRDLHAHPELAYQERRTAGIVAQSLRLLGLEVHEGIGGTG
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
VVGTLRMGTGSRRVGLRADMDALPMVELGRAPHASRHAGAHHGCGHDGHTSMLIGAARQL
CEEEEEECCCCCCCCCCCCCCCCCHHHHCCCCCCHHCCCCCCCCCCCCCHHHHHHHHHHH
ARAGGLDGTVHFIFQPAEEGQGGARRMIEDGLFERFPCDSVYALHNWPDLPLGAARTRPG
HHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCC
PIMAAADRFDIAVRGRGGHAAQPHHTPDAILAASQLVAQLNTTVSRRIDPGESAVLSVTR
CEEEECCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEE
IEGGQSHNVLPAEVRITGTVRSFDPHAQDGIEAALRAACEGIALASGTAVEVDYVRYYPA
ECCCCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHCCCEEECCCEEEEEEEEEECC
TINTAPEAALALEAAAAIGLEAEVAPRAAFTSEDFAFMLQRRPGAYLWLGQGRADPGPEG
CCCCCCHHHHHHHHHHHHCCCCCCCCCHHCCCHHHHHHHHCCCCCEEEEECCCCCCCCCC
ERPLHHPCYDFNDEALGLGVRWFCEVARRALSG
CCCCCCCCCCCCCCHHHCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SLEDVARPVADLLPDLVTLRRDLHAHPELAYQERRTAGIVAQSLRLLGLEVHEGIGGTG
CHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
VVGTLRMGTGSRRVGLRADMDALPMVELGRAPHASRHAGAHHGCGHDGHTSMLIGAARQL
CEEEEEECCCCCCCCCCCCCCCCCHHHHCCCCCCHHCCCCCCCCCCCCCHHHHHHHHHHH
ARAGGLDGTVHFIFQPAEEGQGGARRMIEDGLFERFPCDSVYALHNWPDLPLGAARTRPG
HHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCC
PIMAAADRFDIAVRGRGGHAAQPHHTPDAILAASQLVAQLNTTVSRRIDPGESAVLSVTR
CEEEECCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEE
IEGGQSHNVLPAEVRITGTVRSFDPHAQDGIEAALRAACEGIALASGTAVEVDYVRYYPA
ECCCCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHCCCEEECCCEEEEEEEEEECC
TINTAPEAALALEAAAAIGLEAEVAPRAAFTSEDFAFMLQRRPGAYLWLGQGRADPGPEG
CCCCCCHHHHHHHHHHHHCCCCCCCCCHHCCCHHHHHHHHCCCCCEEEEECCCCCCCCCC
ERPLHHPCYDFNDEALGLGVRWFCEVARRALSG
CCCCCCCCCCCCCCHHHCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7730270; 10688204 [H]