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The map label for this gene is dmlR [H]

Identifier: 120610766

GI number: 120610766

Start: 2291986

End: 2293014

Strand: Direct

Name: dmlR [H]

Synonym: Aave_2088

Alternate gene names: 120610766

Gene position: 2291986-2293014 (Clockwise)

Preceding gene: 120610762

Following gene: 120610767

Centisome position: 42.82

GC content: 70.65

Gene sequence:

>1029_bases
ATGGACGGGAAGGCCGGCACGCCAAAGCGCTGGCCGCCCGCATGCCGCCGATTCTTCGGCAAACCGCATCGCAATACAAT
CCGCCACCCGTGGTTCCATTGTTCACATATCGTGCATAAAACGCCCGCCACCGACGACCTGCGTGTTTTCACCGCGGTGG
TCCGCAAGGCCAGCTTCGCCGGGGCGGCCGCCGAACTGGGCGCCTCGCCTGCCTACGTGAGCAAGCGCATCCGCCTGCTC
GAGGAAGAGCTGGCCGTGAAGCTGCTGCACCGCACGACGCGCCGGGTGGCGGTGACGGAGGAGGGTGAGCGCGTCTTCCA
CTGGGCGCAGCGCATCCTGGACGATTGGGACCAGTTGCTGCAGGAGGTGGCCGTGACGCGGCGCGAGCCGCGCGGGTTGC
TCCGGGTGAGCTGCAGTTTCGGTTTCGGCCGGCGCATCGTGGCGCCCGCGGTGTCGCGGCTGGTGGAGCGCCACCCGGGC
CTGCAGGTGCGGCTCGAAGTGTTCGACCGGCTGGTGGACGTGGCGGGGGAGGGGTTCGACCTGGACATCCGCGTGGGCGA
CGAGATCGCGCCGCACCTGATCGCACGCCGCCTGGCGGGCAACCACCGCGTGCTGTGCGCGGCGCCCGGCTACCTGGCGC
GCAAGGGCACGCCCCGGGCGGTGGACGACCTGGCCGGCCACGACTGCCTGGTGATCAAGGAGCGTGACCATCCCTTCGGC
GTCTGGCGGCTGCGCAGCGGCGCGGCGGACCGGACGGTGAAGGTGCGCGGGTCGCTGTCGGCCAACCATGGCGAAATGGC
CGTGCAGTGGGCCGTGGATGGGCGCGGCATCGTGCTGCGCTCCCTGTGGGATGTCGGGCCCGACCTGGCCACCGGCCGGC
TGGTGCGCGTGCTGCCCGAATGGCAGCAGGAAGCCAACGTCTGGGCCGTGTATCCCACGCGGCTGGAGCGCTCGGCCAAG
GTGCGCGTGTGCGTCGAATTCCTGCAGGCCCATTTCTCGCGGGAAACGTGGGGGAGCGAGGCCGCATGA

Upstream 100 bases:

>100_bases
ATGACTTCCTTGCCGATGCCGTCGCCGGGCAGGACGGCGATCTTGAAGGTGCGGGAGGGGGTGGTCATGGGCGAAGTCTC
CGGAAAAAAGCGTGGAAAGG

Downstream 100 bases:

>100_bases
GCCGTCCCGTCCGGTCCGCCCGGTTGCTGCAGTTGCTGGACGAATTGCGCCGCTGCCGCCACCCCGTCTCTGGCGCGCTG
CTGGCCGAGCGCCTGGGCGT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MDGKAGTPKRWPPACRRFFGKPHRNTIRHPWFHCSHIVHKTPATDDLRVFTAVVRKASFAGAAAELGASPAYVSKRIRLL
EEELAVKLLHRTTRRVAVTEEGERVFHWAQRILDDWDQLLQEVAVTRREPRGLLRVSCSFGFGRRIVAPAVSRLVERHPG
LQVRLEVFDRLVDVAGEGFDLDIRVGDEIAPHLIARRLAGNHRVLCAAPGYLARKGTPRAVDDLAGHDCLVIKERDHPFG
VWRLRSGAADRTVKVRGSLSANHGEMAVQWAVDGRGIVLRSLWDVGPDLATGRLVRVLPEWQQEANVWAVYPTRLERSAK
VRVCVEFLQAHFSRETWGSEAA

Sequences:

>Translated_342_residues
MDGKAGTPKRWPPACRRFFGKPHRNTIRHPWFHCSHIVHKTPATDDLRVFTAVVRKASFAGAAAELGASPAYVSKRIRLL
EEELAVKLLHRTTRRVAVTEEGERVFHWAQRILDDWDQLLQEVAVTRREPRGLLRVSCSFGFGRRIVAPAVSRLVERHPG
LQVRLEVFDRLVDVAGEGFDLDIRVGDEIAPHLIARRLAGNHRVLCAAPGYLARKGTPRAVDDLAGHDCLVIKERDHPFG
VWRLRSGAADRTVKVRGSLSANHGEMAVQWAVDGRGIVLRSLWDVGPDLATGRLVRVLPEWQQEANVWAVYPTRLERSAK
VRVCVEFLQAHFSRETWGSEAA
>Mature_342_residues
MDGKAGTPKRWPPACRRFFGKPHRNTIRHPWFHCSHIVHKTPATDDLRVFTAVVRKASFAGAAAELGASPAYVSKRIRLL
EEELAVKLLHRTTRRVAVTEEGERVFHWAQRILDDWDQLLQEVAVTRREPRGLLRVSCSFGFGRRIVAPAVSRLVERHPG
LQVRLEVFDRLVDVAGEGFDLDIRVGDEIAPHLIARRLAGNHRVLCAAPGYLARKGTPRAVDDLAGHDCLVIKERDHPFG
VWRLRSGAADRTVKVRGSLSANHGEMAVQWAVDGRGIVLRSLWDVGPDLATGRLVRVLPEWQQEANVWAVYPTRLERSAK
VRVCVEFLQAHFSRETWGSEAA

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=296, Percent_Identity=57.7702702702703, Blast_Score=352, Evalue=2e-98,
Organism=Escherichia coli, GI1789440, Length=294, Percent_Identity=48.9795918367347, Blast_Score=309, Evalue=2e-85,
Organism=Escherichia coli, GI1786401, Length=291, Percent_Identity=32.9896907216495, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI1789639, Length=288, Percent_Identity=30.9027777777778, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI145693193, Length=288, Percent_Identity=32.9861111111111, Blast_Score=131, Evalue=7e-32,
Organism=Escherichia coli, GI1787128, Length=288, Percent_Identity=28.8194444444444, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1787589, Length=268, Percent_Identity=27.9850746268657, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1786448, Length=249, Percent_Identity=29.3172690763052, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1789173, Length=257, Percent_Identity=28.0155642023346, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1788706, Length=255, Percent_Identity=28.2352941176471, Blast_Score=82, Evalue=5e-17,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 38481; Mature: 38481

Theoretical pI: Translated: 10.60; Mature: 10.60

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDGKAGTPKRWPPACRRFFGKPHRNTIRHPWFHCSHIVHKTPATDDLRVFTAVVRKASFA
CCCCCCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHC
GAAAELGASPAYVSKRIRLLEEELAVKLLHRTTRRVAVTEEGERVFHWAQRILDDWDQLL
CHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHH
QEVAVTRREPRGLLRVSCSFGFGRRIVAPAVSRLVERHPGLQVRLEVFDRLVDVAGEGFD
HHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCEE
LDIRVGDEIAPHLIARRLAGNHRVLCAAPGYLARKGTPRAVDDLAGHDCLVIKERDHPFG
EEEEECCHHHHHHHHHHHCCCCEEEEECCCHHHCCCCCCHHHHHCCCCEEEEEECCCCCE
VWRLRSGAADRTVKVRGSLSANHGEMAVQWAVDGRGIVLRSLWDVGPDLATGRLVRVLPE
EEEECCCCCCCEEEEEECCCCCCCCEEEEEEECCCCHHHHHHHHCCCCCHHCHHHHHHHH
WQQEANVWAVYPTRLERSAKVRVCVEFLQAHFSRETWGSEAA
HHHCCCEEEEEHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MDGKAGTPKRWPPACRRFFGKPHRNTIRHPWFHCSHIVHKTPATDDLRVFTAVVRKASFA
CCCCCCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHC
GAAAELGASPAYVSKRIRLLEEELAVKLLHRTTRRVAVTEEGERVFHWAQRILDDWDQLL
CHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHH
QEVAVTRREPRGLLRVSCSFGFGRRIVAPAVSRLVERHPGLQVRLEVFDRLVDVAGEGFD
HHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCEE
LDIRVGDEIAPHLIARRLAGNHRVLCAAPGYLARKGTPRAVDDLAGHDCLVIKERDHPFG
EEEEECCHHHHHHHHHHHCCCCEEEEECCCHHHCCCCCCHHHHHCCCCEEEEEECCCCCE
VWRLRSGAADRTVKVRGSLSANHGEMAVQWAVDGRGIVLRSLWDVGPDLATGRLVRVLPE
EEEECCCCCCCEEEEEECCCCCCCCEEEEEEECCCCHHHHHHHHCCCCCHHCHHHHHHHH
WQQEANVWAVYPTRLERSAKVRVCVEFLQAHFSRETWGSEAA
HHHCCCEEEEEHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]