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The map label for this gene is glnG [H]

Identifier: 120610132

GI number: 120610132

Start: 1592112

End: 1593761

Strand: Direct

Name: glnG [H]

Synonym: Aave_1445

Alternate gene names: 120610132

Gene position: 1592112-1593761 (Clockwise)

Preceding gene: 120610131

Following gene: 120610133

Centisome position: 29.74

GC content: 68.06

Gene sequence:

>1650_bases
ATGAAGCCGATCTGGATAGTAGATGACGACCCCTCGATCCGCTTCGTCCTGGAAAAGGCGCTGGCCCGAGAGAACCTGCC
GACACGCAGTTTCACCCACCCGCGCGAGGTGCTTGATGCGCTGGCCGACGTAACGGCCGGAGACCCTGCGCGCCAGGGGC
CGCAGGTGCTGGTCAGCGACATCCGCATGCCCGGGGGCTCGGGCCTGCAGTTGCTGGAGCAGGTGCGGCAGCAGCAACCT
GGCCTGCCGGTCATCATCATGACCGCGTATTCCGATCTCGACAGCGCGGTGTCCGCGTTCCAGCGCGGTGCTTTTGAATA
CCTGCCCAAGCCGTTCGACCTGCCCAAGGCGGTGGAGCTGATCCGCCGTGCGGTCGAGGAAAGCCAGCGGGAAGAGGTCA
CGGAGGAGCGCCAGACCGCCACGCCCGAGATGCTGGGCCAGGCGCCTGCCATGCAGGACGTATTCCGTGCGATCGGGCGG
CTGAGCCAGAGTCAGGTGACGGTGCTGATCACCGGCGAGTCCGGCTCCGGCAAGGAGCTCGTGGCCCGGGCGCTGCACAA
GCATTCGCCGGTGTCCGAAGGGCCCTTCGTCGCGATCAACACGGCGGCGATCCCCAAGGACTTGCTGGAGAGCGAACTGT
TCGGCCATGAACGGGGTGCATTCACCGGCGCCCAGACCCAGCGGCGCGGCCGGTTCGAGCAGGCGGAAGGGGGAACGCTG
TTCCTCGATGAAATCGGTGACATGCCGTTCGACCTGCAGACGCGCCTGCTGCGGGTGCTGTCGGACGGGCAGTTCTATCG
GGTGGGGGGACATGCCGCGGTGAAGGCGCACGTCCGCGTCATTGCCGCAACGCACCAGAACCTGGAGCTGCGCGTCAAGG
ACGGGGTGTTCCGCGAGGATCTTTTCCACCGCCTCAACGTGATCCGTCTGCGGCTGCCCGCATTGCGCGAGCGGCACGAG
GATGTGCCGATGCTGACGCGGCACTTCCTGCAGCAGAGCGCACGCCAGCTGGGGGTCGAACCGAAGCGTATTTCCGACGC
CGCTTTGGCACGGCTTGGGCAATTCTCGTTCCCGGGCAATGTGCGGCAATTGGAGAACATCTGCCATTGGCTGACAGTGA
TGGCACCCGCCCAGGTGATTTCGCTGCAGGATCTGCCACCCGAGGTGCTGGACGGCCATGCGCCCGTGGATCCGCTGATG
TCCAGCGCAGAGGCTCGCCCGGAGATACCGGCGCCTGCGGCCGGGGGGGGCGATGTGCCCGGCTCCGGCATGCAGGCAGG
GATTTCCCGGGACGAACGCGGCGCCGGGTGGCCTGCAGGTCTGGCGGGCAGTTGGGCGCCCCAGGGCGGCCATACGCCCG
TGCATGCCGCCGCACCCGTGCAGGCGGCGATCGCCCACGGGCCGCTGGGCGAACCCTCGGGCTGGGAGCATGCGCTGGAG
GCCGAAGCCCAGAAGCTGCTGGCGACGGGGCAGCCGGAGGTGTGGGACGTGCTCACGCGCCGGTTCGAATCGCGCTTGAT
CCGTACGGCGCTGTCCGCGACCCACGGCCGGCGGATGGAGGCCGCGCAGCGTCTGGGCATCGGTCGCAACACGATCACGC
GCAAGATCCAGGAGCTGGGCCTCGGCCCTTCCGACGATCCGGTGTCCTGA

Upstream 100 bases:

>100_bases
TCGAATGCGACAGCGTTCCGGGTCGCACCGATTTCCGCATCCTCATTCCCTTGCCCTGAGGTCCGGAGACATTCACTTCG
AGACAAGGTAGAACTCACAC

Downstream 100 bases:

>100_bases
CACGGATGGGGCATATCCGGTCGCGATCGGTTTGCTCCTGTTTTCGTAGCAATAGGATGAGATTGGGCGAATGTTTGCTC
ATCCGTTCCGCTGGCGCCTC

Product: nitrogen metabolism transcriptional regulator NtrC

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 549; Mature: 549

Protein sequence:

>549_residues
MKPIWIVDDDPSIRFVLEKALARENLPTRSFTHPREVLDALADVTAGDPARQGPQVLVSDIRMPGGSGLQLLEQVRQQQP
GLPVIIMTAYSDLDSAVSAFQRGAFEYLPKPFDLPKAVELIRRAVEESQREEVTEERQTATPEMLGQAPAMQDVFRAIGR
LSQSQVTVLITGESGSGKELVARALHKHSPVSEGPFVAINTAAIPKDLLESELFGHERGAFTGAQTQRRGRFEQAEGGTL
FLDEIGDMPFDLQTRLLRVLSDGQFYRVGGHAAVKAHVRVIAATHQNLELRVKDGVFREDLFHRLNVIRLRLPALRERHE
DVPMLTRHFLQQSARQLGVEPKRISDAALARLGQFSFPGNVRQLENICHWLTVMAPAQVISLQDLPPEVLDGHAPVDPLM
SSAEARPEIPAPAAGGGDVPGSGMQAGISRDERGAGWPAGLAGSWAPQGGHTPVHAAAPVQAAIAHGPLGEPSGWEHALE
AEAQKLLATGQPEVWDVLTRRFESRLIRTALSATHGRRMEAAQRLGIGRNTITRKIQELGLGPSDDPVS

Sequences:

>Translated_549_residues
MKPIWIVDDDPSIRFVLEKALARENLPTRSFTHPREVLDALADVTAGDPARQGPQVLVSDIRMPGGSGLQLLEQVRQQQP
GLPVIIMTAYSDLDSAVSAFQRGAFEYLPKPFDLPKAVELIRRAVEESQREEVTEERQTATPEMLGQAPAMQDVFRAIGR
LSQSQVTVLITGESGSGKELVARALHKHSPVSEGPFVAINTAAIPKDLLESELFGHERGAFTGAQTQRRGRFEQAEGGTL
FLDEIGDMPFDLQTRLLRVLSDGQFYRVGGHAAVKAHVRVIAATHQNLELRVKDGVFREDLFHRLNVIRLRLPALRERHE
DVPMLTRHFLQQSARQLGVEPKRISDAALARLGQFSFPGNVRQLENICHWLTVMAPAQVISLQDLPPEVLDGHAPVDPLM
SSAEARPEIPAPAAGGGDVPGSGMQAGISRDERGAGWPAGLAGSWAPQGGHTPVHAAAPVQAAIAHGPLGEPSGWEHALE
AEAQKLLATGQPEVWDVLTRRFESRLIRTALSATHGRRMEAAQRLGIGRNTITRKIQELGLGPSDDPVS
>Mature_549_residues
MKPIWIVDDDPSIRFVLEKALARENLPTRSFTHPREVLDALADVTAGDPARQGPQVLVSDIRMPGGSGLQLLEQVRQQQP
GLPVIIMTAYSDLDSAVSAFQRGAFEYLPKPFDLPKAVELIRRAVEESQREEVTEERQTATPEMLGQAPAMQDVFRAIGR
LSQSQVTVLITGESGSGKELVARALHKHSPVSEGPFVAINTAAIPKDLLESELFGHERGAFTGAQTQRRGRFEQAEGGTL
FLDEIGDMPFDLQTRLLRVLSDGQFYRVGGHAAVKAHVRVIAATHQNLELRVKDGVFREDLFHRLNVIRLRLPALRERHE
DVPMLTRHFLQQSARQLGVEPKRISDAALARLGQFSFPGNVRQLENICHWLTVMAPAQVISLQDLPPEVLDGHAPVDPLM
SSAEARPEIPAPAAGGGDVPGSGMQAGISRDERGAGWPAGLAGSWAPQGGHTPVHAAAPVQAAIAHGPLGEPSGWEHALE
AEAQKLLATGQPEVWDVLTRRFESRLIRTALSATHGRRMEAAQRLGIGRNTITRKIQELGLGPSDDPVS

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=540, Percent_Identity=53.1481481481481, Blast_Score=527, Evalue=1e-151,
Organism=Escherichia coli, GI1788550, Length=392, Percent_Identity=44.1326530612245, Blast_Score=344, Evalue=9e-96,
Organism=Escherichia coli, GI1790437, Length=434, Percent_Identity=38.4792626728111, Blast_Score=290, Evalue=2e-79,
Organism=Escherichia coli, GI1788905, Length=386, Percent_Identity=41.7098445595855, Blast_Score=283, Evalue=3e-77,
Organism=Escherichia coli, GI87082117, Length=225, Percent_Identity=52.4444444444444, Blast_Score=239, Evalue=5e-64,
Organism=Escherichia coli, GI1789087, Length=248, Percent_Identity=47.9838709677419, Blast_Score=228, Evalue=1e-60,
Organism=Escherichia coli, GI1789233, Length=252, Percent_Identity=44.8412698412698, Blast_Score=216, Evalue=3e-57,
Organism=Escherichia coli, GI87082152, Length=235, Percent_Identity=47.6595744680851, Blast_Score=214, Evalue=1e-56,
Organism=Escherichia coli, GI1786524, Length=305, Percent_Identity=41.9672131147541, Blast_Score=202, Evalue=3e-53,
Organism=Escherichia coli, GI87081872, Length=225, Percent_Identity=42.6666666666667, Blast_Score=185, Evalue=8e-48,
Organism=Escherichia coli, GI1787583, Length=258, Percent_Identity=41.4728682170543, Blast_Score=184, Evalue=2e-47,
Organism=Escherichia coli, GI1789828, Length=239, Percent_Identity=34.3096234309623, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI87081858, Length=278, Percent_Identity=30.9352517985612, Blast_Score=121, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 59808; Mature: 59808

Theoretical pI: Translated: 6.47; Mature: 6.47

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPIWIVDDDPSIRFVLEKALARENLPTRSFTHPREVLDALADVTAGDPARQGPQVLVSD
CCCEEEECCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHH
IRMPGGSGLQLLEQVRQQQPGLPVIIMTAYSDLDSAVSAFQRGAFEYLPKPFDLPKAVEL
HCCCCCCCHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHCHHHHCCCCCCCHHHHHH
IRRAVEESQREEVTEERQTATPEMLGQAPAMQDVFRAIGRLSQSQVTVLITGESGSGKEL
HHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCHHH
VARALHKHSPVSEGPFVAINTAAIPKDLLESELFGHERGAFTGAQTQRRGRFEQAEGGTL
HHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCCCEE
FLDEIGDMPFDLQTRLLRVLSDGQFYRVGGHAAVKAHVRVIAATHQNLELRVKDGVFRED
EHHHHCCCCHHHHHHHHHHHCCCCEEEECCCHHHHHHHHEEEEECCCCEEEECCCCHHHH
LFHRLNVIRLRLPALRERHEDVPMLTRHFLQQSARQLGVEPKRISDAALARLGQFSFPGN
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCC
VRQLENICHWLTVMAPAQVISLQDLPPEVLDGHAPVDPLMSSAEARPEIPAPAAGGGDVP
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCCC
GSGMQAGISRDERGAGWPAGLAGSWAPQGGHTPVHAAAPVQAAIAHGPLGEPSGWEHALE
CCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCHHHHHH
AEAQKLLATGQPEVWDVLTRRFESRLIRTALSATHGRRMEAAQRLGIGRNTITRKIQELG
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHC
LGPSDDPVS
CCCCCCCCC
>Mature Secondary Structure
MKPIWIVDDDPSIRFVLEKALARENLPTRSFTHPREVLDALADVTAGDPARQGPQVLVSD
CCCEEEECCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHH
IRMPGGSGLQLLEQVRQQQPGLPVIIMTAYSDLDSAVSAFQRGAFEYLPKPFDLPKAVEL
HCCCCCCCHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHCHHHHCCCCCCCHHHHHH
IRRAVEESQREEVTEERQTATPEMLGQAPAMQDVFRAIGRLSQSQVTVLITGESGSGKEL
HHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCHHH
VARALHKHSPVSEGPFVAINTAAIPKDLLESELFGHERGAFTGAQTQRRGRFEQAEGGTL
HHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCCCEE
FLDEIGDMPFDLQTRLLRVLSDGQFYRVGGHAAVKAHVRVIAATHQNLELRVKDGVFRED
EHHHHCCCCHHHHHHHHHHHCCCCEEEECCCHHHHHHHHEEEEECCCCEEEECCCCHHHH
LFHRLNVIRLRLPALRERHEDVPMLTRHFLQQSARQLGVEPKRISDAALARLGQFSFPGN
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCC
VRQLENICHWLTVMAPAQVISLQDLPPEVLDGHAPVDPLMSSAEARPEIPAPAAGGGDVP
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCCC
GSGMQAGISRDERGAGWPAGLAGSWAPQGGHTPVHAAAPVQAAIAHGPLGEPSGWEHALE
CCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCHHHHHH
AEAQKLLATGQPEVWDVLTRRFESRLIRTALSATHGRRMEAAQRLGIGRNTITRKIQELG
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHC
LGPSDDPVS
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]