Click here to switch to the map view.

The map label for this gene is rsuA [H]

Identifier: 120610123

GI number: 120610123

Start: 1583260

End: 1584021

Strand: Direct

Name: rsuA [H]

Synonym: Aave_1436

Alternate gene names: 120610123

Gene position: 1583260-1584021 (Clockwise)

Preceding gene: 120610122

Following gene: 120610124

Centisome position: 29.58

GC content: 56.82

Gene sequence:

>762_bases
TTGGAGATCCAGATTGTGCAATTGCAAGATATCCTGTATTCCCAAGGGTTTGGCACCAGAAGGGTATGCGCCGGCCTGAT
CCAGCATGGCCATGTTCAACTGCGTGAGAAAGGCGATGCTGGCGGAGCATGGACTTGCACCGATGCCGGGCTGGACGTCG
ATCCCGAGGGCTTGACCTTCTATGTTCAGGGCACGGCTTGGCAATGGCACGAGCGGGCCTACGTCATGATGAACAAGCCG
GCGGCCGTGGAGTGTTCACAGAAACCATCCACTTATCCCAGCGTCTATACCTTGCTGCCTGACCCATTGCGGCAGCGGCC
AGGACCGGGAAAGATCCTCGGCGTGCAGACCATCGGACGGTTGGACCAAGACACGACGGGACTGTTGCTGCTGAGCGATG
ACGGCAGTTTCATCCACCGCGTGAGCTCTCCGAAGAAACAAATACAAAAGACCTACAGGGTGCACGCCAAGTATGATCTT
TCTCAACAGCAGAAGGATCAGCTACTGAAGGGCGTCGTATTGAACGATGATCCGAAACCGGTGATCGCTGTGGACTGCGT
CCTCCAGGAAGGCCCGGTTTTCGATCTCACGGTCACCGAAGGAAAGTACCACCAGATCAAGCGGATGGTGGCTGCCGTGG
GTAACCGTGTGGAGCAGTTGCACCGCATCCGCATCGGTGGCTTGCATTTGCCCGATGATCTGCAGGCTGGTGATTGGCGG
TGGCTCCGTGAAGAGGATGTCCGCAGGATCTTTGAAGGTTGA

Upstream 100 bases:

>100_bases
CCGATGATAGTGCGGGTTCCCGTGTGAAAGTAGGTCATCGTCAGGCTCCTACAGCCCAAACCGCCCTCAGATCATCATTG
GTCTGAGGGCGTTTTGCTTT

Downstream 100 bases:

>100_bases
GCCTCGGGCGTGGCGCTTGGATGTGCAGTGAAGATGGTGGCGTGGTAACGGCTTGGATATGAATATGCGCAAGTGCCCTC
GGTGGCTTTTTTCGATCGTG

Product: ribosomal small subunit pseudouridine synthase A

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: 16S pseudouridine 516 synthase; 16S pseudouridylate 516 synthase; rRNA pseudouridylate synthase A; rRNA-uridine isomerase A [H]

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MEIQIVQLQDILYSQGFGTRRVCAGLIQHGHVQLREKGDAGGAWTCTDAGLDVDPEGLTFYVQGTAWQWHERAYVMMNKP
AAVECSQKPSTYPSVYTLLPDPLRQRPGPGKILGVQTIGRLDQDTTGLLLLSDDGSFIHRVSSPKKQIQKTYRVHAKYDL
SQQQKDQLLKGVVLNDDPKPVIAVDCVLQEGPVFDLTVTEGKYHQIKRMVAAVGNRVEQLHRIRIGGLHLPDDLQAGDWR
WLREEDVRRIFEG

Sequences:

>Translated_253_residues
MEIQIVQLQDILYSQGFGTRRVCAGLIQHGHVQLREKGDAGGAWTCTDAGLDVDPEGLTFYVQGTAWQWHERAYVMMNKP
AAVECSQKPSTYPSVYTLLPDPLRQRPGPGKILGVQTIGRLDQDTTGLLLLSDDGSFIHRVSSPKKQIQKTYRVHAKYDL
SQQQKDQLLKGVVLNDDPKPVIAVDCVLQEGPVFDLTVTEGKYHQIKRMVAAVGNRVEQLHRIRIGGLHLPDDLQAGDWR
WLREEDVRRIFEG
>Mature_253_residues
MEIQIVQLQDILYSQGFGTRRVCAGLIQHGHVQLREKGDAGGAWTCTDAGLDVDPEGLTFYVQGTAWQWHERAYVMMNKP
AAVECSQKPSTYPSVYTLLPDPLRQRPGPGKILGVQTIGRLDQDTTGLLLLSDDGSFIHRVSSPKKQIQKTYRVHAKYDL
SQQQKDQLLKGVVLNDDPKPVIAVDCVLQEGPVFDLTVTEGKYHQIKRMVAAVGNRVEQLHRIRIGGLHLPDDLQAGDWR
WLREEDVRRIFEG

Specific function: Responsible for synthesis of pseudouridine from uracil- 516 in 16S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=244, Percent_Identity=35.655737704918, Blast_Score=131, Evalue=3e-32,
Organism=Escherichia coli, GI1787524, Length=238, Percent_Identity=28.9915966386555, Blast_Score=94, Evalue=6e-21,
Organism=Escherichia coli, GI87081838, Length=146, Percent_Identity=31.5068493150685, Blast_Score=68, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70

Molecular weight: Translated: 28478; Mature: 28478

Theoretical pI: Translated: 7.23; Mature: 7.23

Prosite motif: PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIQIVQLQDILYSQGFGTRRVCAGLIQHGHVQLREKGDAGGAWTCTDAGLDVDPEGLTF
CEEEEEEHHHHHHHCCCCHHHHHHHHHHCCCEEEEECCCCCCEEEEECCCCCCCCCCEEE
YVQGTAWQWHERAYVMMNKPAAVECSQKPSTYPSVYTLLPDPLRQRPGPGKILGVQTIGR
EEECCEEEECCEEEEEECCCCEEEECCCCCCCCEEEEECCCHHHCCCCCCCEEEEHHHCC
LDQDTTGLLLLSDDGSFIHRVSSPKKQIQKTYRVHAKYDLSQQQKDQLLKGVVLNDDPKP
CCCCCCEEEEECCCCCEEECCCCHHHHHHHHHHHHEEECCCHHHHHHHHCCEEECCCCCC
VIAVDCVLQEGPVFDLTVTEGKYHQIKRMVAAVGNRVEQLHRIRIGGLHLPDDLQAGDWR
EEEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCEE
WLREEDVRRIFEG
EHHHHHHHHHHCC
>Mature Secondary Structure
MEIQIVQLQDILYSQGFGTRRVCAGLIQHGHVQLREKGDAGGAWTCTDAGLDVDPEGLTF
CEEEEEEHHHHHHHCCCCHHHHHHHHHHCCCEEEEECCCCCCEEEEECCCCCCCCCCEEE
YVQGTAWQWHERAYVMMNKPAAVECSQKPSTYPSVYTLLPDPLRQRPGPGKILGVQTIGR
EEECCEEEECCEEEEEECCCCEEEECCCCCCCCEEEEECCCHHHCCCCCCCEEEEHHHCC
LDQDTTGLLLLSDDGSFIHRVSSPKKQIQKTYRVHAKYDLSQQQKDQLLKGVVLNDDPKP
CCCCCCEEEEECCCCCEEECCCCHHHHHHHHHHHHEEECCCHHHHHHHHCCEEECCCCCC
VIAVDCVLQEGPVFDLTVTEGKYHQIKRMVAAVGNRVEQLHRIRIGGLHLPDDLQAGDWR
EEEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCEE
WLREEDVRRIFEG
EHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]