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The map label for this gene is purA

Identifier: 120610121

GI number: 120610121

Start: 1575516

End: 1576895

Strand: Direct

Name: purA

Synonym: Aave_1434

Alternate gene names: 120610121

Gene position: 1575516-1576895 (Clockwise)

Preceding gene: 120610120

Following gene: 120610122

Centisome position: 29.43

GC content: 65.29

Gene sequence:

>1380_bases
ATGCAAGCAGCGATCAAAGGGCGAAACGTCGTCGTGGTGGGCACCCAGTGGGGCGACGAAGGCAAGGGCAAGCTCGTGGA
CTGGCTCACCGAAAGCGCCCAGGGCGTGGTGCGCTTCCAGGGCGGCCACAACGCGGGCCACACCCTCGTCATCAACGGCG
TGAAGACCGCGCTGCACCTCATCCCCAGCGGCATCATGCGGCCCGGGGTCAAGTGCTACATCGGCAATGGCGTCGTCCTC
TCGGCGGCCAAGCTGTTCGAGGAAATCGAAGGGCTGGAGAAGGCCGGAGTGGAAGTGCGTTCCCGGCTGCGCATCAGCGA
GGCATGCCCGCTGATTCTGCCGTTCCACGCCGCACTGGATGTCGCCCGCGAGGCCGCGCGCGAACATGGCGGCAGCGAGA
AGATCGGTACGACCGGCCGTGGCATCGGCCCCGCCTATGAAGACAAGGTGGCGCGCCGCGCCCTCCGCGTCCAGGATCTC
AAGCATCCCGAGCGCTTCGCCGCGAAGCTCAAGGAACTGCTGGCGCTCCACAACCATGTGCTGAAGTCCTTCCTGCACTC
CGGATCCTTCCAGTTCGGCAGCGCACTGCAGCCCTATCTGAAGGATGGAGAGGTGCAGTTCGACGCGGTCTACCAGGAAG
CGATGCGCCATGCCGAGCTGCTCAAGCCCATGATCGCCGACGTATCCCGCGAACTGAACGAAGCCCACAAGGCGGGCGCC
AACCTTCTGTTCGAAGGTGCGCAAGGCACGCTGCTGGACGTGGACCATGGCACTTACCCCTATGTGACTTCCAGCAACTG
CGTTGCCGGCAACGCGGCGGCAGGATCCGGCGTGGGCCCAGGCCTGCTGCACTACGTGCTGGGTATCACGAAAGCCTATT
GCACCCGGGTGGGCGGCGGGCCGTTCCCGACCGAGCTGGAATGGGAAAAGCCGGGCACCCCGGGCTACCACATGAGCACC
ATCGGCGCCGAAAAAGGCGTGACCACCGGCCGCAGCCGGCGCTGCGGCTGGTTCGATGCCGCACTGCTCAAGAGAAGCGC
CCAGGTGAATGGCCTGACCGGCCTGTGCATCACCAAGCTGGACGTGCTGGACGGCCTGGAGGAACTGAAGCTCTGCGTCG
GCTACGAACTCGATGGCGAGCGCATCGACATCCTGCCCATGGGGGCCGAGGAAATCGCCCGCTGCGTTCCCGTGTATGAA
ACCCTGGCGGGCTGGAGTGATTCCACCGTCGGCGTCACCCAATACGACTCGCTTCCTGCCAATGCGCGCCGCTACCTGGA
GCGCATCGCCGAAGTGACCGCCGTGCCCATCGCCATGATCTCCACGAGTCCTGACCGGGACCACACGATCATGATGCAGC
ACCCCTACGCCGCGCAGTGA

Upstream 100 bases:

>100_bases
GCAGGAATTCCAGTGCGACCGCGAGCTGGTGCTCGAGCAGGGCCAGTGGATGGTGCGGCCTCTTCCATATTGATGAACAT
TGAATCCAGGCAGGTTTGAC

Downstream 100 bases:

>100_bases
GCGCGGTGCGCCATATCCGTTTTTCCTGAAAGCGCATTCCGGAGCCCCTCACCCATGTTGACTGAAGACGGCAAGCACCT
CTATGTGAGCTACGACGAGT

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MQAAIKGRNVVVVGTQWGDEGKGKLVDWLTESAQGVVRFQGGHNAGHTLVINGVKTALHLIPSGIMRPGVKCYIGNGVVL
SAAKLFEEIEGLEKAGVEVRSRLRISEACPLILPFHAALDVAREAAREHGGSEKIGTTGRGIGPAYEDKVARRALRVQDL
KHPERFAAKLKELLALHNHVLKSFLHSGSFQFGSALQPYLKDGEVQFDAVYQEAMRHAELLKPMIADVSRELNEAHKAGA
NLLFEGAQGTLLDVDHGTYPYVTSSNCVAGNAAAGSGVGPGLLHYVLGITKAYCTRVGGGPFPTELEWEKPGTPGYHMST
IGAEKGVTTGRSRRCGWFDAALLKRSAQVNGLTGLCITKLDVLDGLEELKLCVGYELDGERIDILPMGAEEIARCVPVYE
TLAGWSDSTVGVTQYDSLPANARRYLERIAEVTAVPIAMISTSPDRDHTIMMQHPYAAQ

Sequences:

>Translated_459_residues
MQAAIKGRNVVVVGTQWGDEGKGKLVDWLTESAQGVVRFQGGHNAGHTLVINGVKTALHLIPSGIMRPGVKCYIGNGVVL
SAAKLFEEIEGLEKAGVEVRSRLRISEACPLILPFHAALDVAREAAREHGGSEKIGTTGRGIGPAYEDKVARRALRVQDL
KHPERFAAKLKELLALHNHVLKSFLHSGSFQFGSALQPYLKDGEVQFDAVYQEAMRHAELLKPMIADVSRELNEAHKAGA
NLLFEGAQGTLLDVDHGTYPYVTSSNCVAGNAAAGSGVGPGLLHYVLGITKAYCTRVGGGPFPTELEWEKPGTPGYHMST
IGAEKGVTTGRSRRCGWFDAALLKRSAQVNGLTGLCITKLDVLDGLEELKLCVGYELDGERIDILPMGAEEIARCVPVYE
TLAGWSDSTVGVTQYDSLPANARRYLERIAEVTAVPIAMISTSPDRDHTIMMQHPYAAQ
>Mature_459_residues
MQAAIKGRNVVVVGTQWGDEGKGKLVDWLTESAQGVVRFQGGHNAGHTLVINGVKTALHLIPSGIMRPGVKCYIGNGVVL
SAAKLFEEIEGLEKAGVEVRSRLRISEACPLILPFHAALDVAREAAREHGGSEKIGTTGRGIGPAYEDKVARRALRVQDL
KHPERFAAKLKELLALHNHVLKSFLHSGSFQFGSALQPYLKDGEVQFDAVYQEAMRHAELLKPMIADVSRELNEAHKAGA
NLLFEGAQGTLLDVDHGTYPYVTSSNCVAGNAAAGSGVGPGLLHYVLGITKAYCTRVGGGPFPTELEWEKPGTPGYHMST
IGAEKGVTTGRSRRCGWFDAALLKRSAQVNGLTGLCITKLDVLDGLEELKLCVGYELDGERIDILPMGAEEIARCVPVYE
TLAGWSDSTVGVTQYDSLPANARRYLERIAEVTAVPIAMISTSPDRDHTIMMQHPYAAQ

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI22748717, Length=453, Percent_Identity=39.9558498896247, Blast_Score=316, Evalue=3e-86,
Organism=Homo sapiens, GI34577063, Length=450, Percent_Identity=40, Blast_Score=313, Evalue=1e-85,
Organism=Homo sapiens, GI40316944, Length=416, Percent_Identity=38.4615384615385, Blast_Score=278, Evalue=9e-75,
Organism=Escherichia coli, GI1790620, Length=453, Percent_Identity=56.5121412803532, Blast_Score=490, Evalue=1e-140,
Organism=Caenorhabditis elegans, GI25146553, Length=452, Percent_Identity=41.3716814159292, Blast_Score=315, Evalue=3e-86,
Organism=Caenorhabditis elegans, GI25146555, Length=452, Percent_Identity=41.3716814159292, Blast_Score=315, Evalue=4e-86,
Organism=Saccharomyces cerevisiae, GI6324109, Length=449, Percent_Identity=41.2026726057906, Blast_Score=335, Evalue=1e-92,
Organism=Drosophila melanogaster, GI21356033, Length=448, Percent_Identity=39.2857142857143, Blast_Score=303, Evalue=2e-82,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_ACIAC (A1TM37)

Other databases:

- EMBL:   CP000512
- RefSeq:   YP_969799.1
- ProteinModelPortal:   A1TM37
- SMR:   A1TM37
- STRING:   A1TM37
- GeneID:   4669237
- GenomeReviews:   CP000512_GR
- KEGG:   aav:Aave_1434
- NMPDR:   fig|397945.5.peg.1259
- eggNOG:   COG0104
- HOGENOM:   HBG658237
- OMA:   YVLGIIK
- PhylomeDB:   A1TM37
- ProtClustDB:   PRK01117
- BioCyc:   AAVE397945:AAVE_1434-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 49456; Mature: 49456

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 19-19 ACT_SITE 47-47 BINDING 138-138 BINDING 152-152 BINDING 248-248 BINDING 263-263 BINDING 331-331 BINDING 333-333

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAAIKGRNVVVVGTQWGDEGKGKLVDWLTESAQGVVRFQGGHNAGHTLVINGVKTALHL
CCCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCEEEEEHHHHHHHH
IPSGIMRPGVKCYIGNGVVLSAAKLFEEIEGLEKAGVEVRSRLRISEACPLILPFHAALD
HHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCEEEHHHHHHH
VAREAAREHGGSEKIGTTGRGIGPAYEDKVARRALRVQDLKHPERFAAKLKELLALHNHV
HHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
LKSFLHSGSFQFGSALQPYLKDGEVQFDAVYQEAMRHAELLKPMIADVSRELNEAHKAGA
HHHHHHCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NLLFEGAQGTLLDVDHGTYPYVTSSNCVAGNAAAGSGVGPGLLHYVLGITKAYCTRVGGG
CEEEECCCCCEEECCCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
PFPTELEWEKPGTPGYHMSTIGAEKGVTTGRSRRCGWFDAALLKRSAQVNGLTGLCITKL
CCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHH
DVLDGLEELKLCVGYELDGERIDILPMGAEEIARCVPVYETLAGWSDSTVGVTQYDSLPA
HHHCCHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCH
NARRYLERIAEVTAVPIAMISTSPDRDHTIMMQHPYAAQ
HHHHHHHHHHHHHHCCEEEEECCCCCCCEEEECCCCCCC
>Mature Secondary Structure
MQAAIKGRNVVVVGTQWGDEGKGKLVDWLTESAQGVVRFQGGHNAGHTLVINGVKTALHL
CCCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCEEEEEHHHHHHHH
IPSGIMRPGVKCYIGNGVVLSAAKLFEEIEGLEKAGVEVRSRLRISEACPLILPFHAALD
HHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCEEEHHHHHHH
VAREAAREHGGSEKIGTTGRGIGPAYEDKVARRALRVQDLKHPERFAAKLKELLALHNHV
HHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
LKSFLHSGSFQFGSALQPYLKDGEVQFDAVYQEAMRHAELLKPMIADVSRELNEAHKAGA
HHHHHHCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NLLFEGAQGTLLDVDHGTYPYVTSSNCVAGNAAAGSGVGPGLLHYVLGITKAYCTRVGGG
CEEEECCCCCEEECCCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
PFPTELEWEKPGTPGYHMSTIGAEKGVTTGRSRRCGWFDAALLKRSAQVNGLTGLCITKL
CCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHH
DVLDGLEELKLCVGYELDGERIDILPMGAEEIARCVPVYETLAGWSDSTVGVTQYDSLPA
HHHCCHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCH
NARRYLERIAEVTAVPIAMISTSPDRDHTIMMQHPYAAQ
HHHHHHHHHHHHHHCCEEEEECCCCCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA