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The map label for this gene is hisZ

Identifier: 120610120

GI number: 120610120

Start: 1574334

End: 1575488

Strand: Direct

Name: hisZ

Synonym: Aave_1433

Alternate gene names: 120610120

Gene position: 1574334-1575488 (Clockwise)

Preceding gene: 120610119

Following gene: 120610121

Centisome position: 29.41

GC content: 67.36

Gene sequence:

>1155_bases
ATGTCCGCTTGGGTCCTGCCGGATCACATTGCCGATGTCCTGCCTTCGGAGGCCCGGCACATCGAAGAACTGCGTCGAGG
GCTCATCGACACCGCCCGGGGCTATGGCTATGAACTGGTCATGCCCCCGATGTTGGAGCACCTGGAATCCCTGCTGTCCG
GAACGGGGGAGGCGCTCGACCTCCAGACGTTCAAGCTGGTGGATCAGTTGTCCGGCCGCTCCATCGGCCTGCGTGCCGAC
ATGACCCAGCAGGTCGCACGGATCGATGCCCACCTGCTGAACCGCTCCGGTGTGACACGTCTCTGCTATTGCGGCCCCGT
GCTGCACACCCGGCCGGACCGTCCCCGCGCGACCCGCGAACCCCTGCAATTCGGTGCCGAAATCTATGGCCATTCCGGCA
TGGAGGCCGACCTGGAATCCGTGCTGCTGGCACTCGACTGCCTGCACCTCGCAGGCGTCGAGGGCGTCAGCGTCGATCTT
TCCGACGCCCGCGTCGTCCGCACCCTGCTGGAGCCCATCGCCGCGGATTCCGCCACGGTCCGGCGAATCTACGCGGCGCT
CGCTGCCAAGGATGCCTCCGAGCTGTCGCAGGCGTCGGCGGGGCTGCCTTCCGGAACCCGGCGTGCGTTGTCCGCCGTGC
TGGAGCTCTATGGCGACGCCAGCGTCCTGGACGAGGCCCGGACCGCGCTCAGCGACGTGGCCGGTGTCGAAGAGGTGCTC
GCCAACCTGAAATCCATCGCCGCGCATCTCGAGGGCCGCACGGTGTCGTTCGACCTCGCCGATCTGCGCGGCTATTCCTA
TTACAGCGGCGCCCGCTTCACCATCTACGCCCGCAACGCGACCGATGCCGTCGTGCGGGGGGGGCGCTACGACGAAGTGG
GGGCGGCCTTCGGCAGGACCCGGCCGGCCGCGGGCTTCAGCCTCGACATCAAGCAACTGGTCGGCATTGTTCCCCCACGG
ACGCTGAAAGCGGCGATCAGGGCCCCCTGGGCTGCCACGCCGGACGCCCATCGCTTCATTGCACAACTCCGCAGCGCGGG
TGAAACCGTGGTCTGCGTGTTGCCGGGCCACGAAAGCCAGGTGCAGGAATTCCAGTGCGACCGCGAGCTGGTGCTCGAGC
AGGGCCAGTGGATGGTGCGGCCTCTTCCATATTGA

Upstream 100 bases:

>100_bases
GCAGGCTGGCACGCCTGTGCAGCATTCCGCTCGTCCGCTGCGAGACGGCGGCACCGGCAGTGCCCGGTAGAATTGCATTT
TTAACAGACACCCCCTTCCC

Downstream 100 bases:

>100_bases
TGAACATTGAATCCAGGCAGGTTTGACATGCAAGCAGCGATCAAAGGGCGAAACGTCGTCGTGGTGGGCACCCAGTGGGG
CGACGAAGGCAAGGGCAAGC

Product: class II tRNA synthetase

Products: AMP; diphosphate +L-histidyl-tRNAHis [C]

Alternate protein names: NA

Number of amino acids: Translated: 384; Mature: 383

Protein sequence:

>384_residues
MSAWVLPDHIADVLPSEARHIEELRRGLIDTARGYGYELVMPPMLEHLESLLSGTGEALDLQTFKLVDQLSGRSIGLRAD
MTQQVARIDAHLLNRSGVTRLCYCGPVLHTRPDRPRATREPLQFGAEIYGHSGMEADLESVLLALDCLHLAGVEGVSVDL
SDARVVRTLLEPIAADSATVRRIYAALAAKDASELSQASAGLPSGTRRALSAVLELYGDASVLDEARTALSDVAGVEEVL
ANLKSIAAHLEGRTVSFDLADLRGYSYYSGARFTIYARNATDAVVRGGRYDEVGAAFGRTRPAAGFSLDIKQLVGIVPPR
TLKAAIRAPWAATPDAHRFIAQLRSAGETVVCVLPGHESQVQEFQCDRELVLEQGQWMVRPLPY

Sequences:

>Translated_384_residues
MSAWVLPDHIADVLPSEARHIEELRRGLIDTARGYGYELVMPPMLEHLESLLSGTGEALDLQTFKLVDQLSGRSIGLRAD
MTQQVARIDAHLLNRSGVTRLCYCGPVLHTRPDRPRATREPLQFGAEIYGHSGMEADLESVLLALDCLHLAGVEGVSVDL
SDARVVRTLLEPIAADSATVRRIYAALAAKDASELSQASAGLPSGTRRALSAVLELYGDASVLDEARTALSDVAGVEEVL
ANLKSIAAHLEGRTVSFDLADLRGYSYYSGARFTIYARNATDAVVRGGRYDEVGAAFGRTRPAAGFSLDIKQLVGIVPPR
TLKAAIRAPWAATPDAHRFIAQLRSAGETVVCVLPGHESQVQEFQCDRELVLEQGQWMVRPLPY
>Mature_383_residues
SAWVLPDHIADVLPSEARHIEELRRGLIDTARGYGYELVMPPMLEHLESLLSGTGEALDLQTFKLVDQLSGRSIGLRADM
TQQVARIDAHLLNRSGVTRLCYCGPVLHTRPDRPRATREPLQFGAEIYGHSGMEADLESVLLALDCLHLAGVEGVSVDLS
DARVVRTLLEPIAADSATVRRIYAALAAKDASELSQASAGLPSGTRRALSAVLELYGDASVLDEARTALSDVAGVEEVLA
NLKSIAAHLEGRTVSFDLADLRGYSYYSGARFTIYARNATDAVVRGGRYDEVGAAFGRTRPAAGFSLDIKQLVGIVPPRT
LKAAIRAPWAATPDAHRFIAQLRSAGETVVCVLPGHESQVQEFQCDRELVLEQGQWMVRPLPY

Specific function: Required for the first step of histidine biosynthesis. May allow the feedback regulation of ATP phosphoribosyltransferase activity by histidine

COG id: COG3705

COG function: function code E; ATP phosphoribosyltransferase involved in histidine biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. HisZ subfamily

Homologues:

Organism=Escherichia coli, GI1788861, Length=324, Percent_Identity=24.3827160493827, Blast_Score=61, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HISZ_ACIAC (A1TM36)

Other databases:

- EMBL:   CP000512
- RefSeq:   YP_969798.1
- STRING:   A1TM36
- GeneID:   4669141
- GenomeReviews:   CP000512_GR
- KEGG:   aav:Aave_1433
- NMPDR:   fig|397945.5.peg.1258
- eggNOG:   COG3705
- HOGENOM:   HBG616575
- OMA:   DLRGYHY
- BioCyc:   AAVE397945:AAVE_1433-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00125
- InterPro:   IPR004517
- InterPro:   IPR004516
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00443

Pfam domain/function: NA

EC number: 6.1.1.21 [C]

Molecular weight: Translated: 41636; Mature: 41505

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAWVLPDHIADVLPSEARHIEELRRGLIDTARGYGYELVMPPMLEHLESLLSGTGEALD
CCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHCCCCCEEH
LQTFKLVDQLSGRSIGLRADMTQQVARIDAHLLNRSGVTRLCYCGPVLHTRPDRPRATRE
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCHHCCCCCCCCCHHH
PLQFGAEIYGHSGMEADLESVLLALDCLHLAGVEGVSVDLSDARVVRTLLEPIAADSATV
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCEEECHHHHHHHHHHHHHCCCHHHH
RRIYAALAAKDASELSQASAGLPSGTRRALSAVLELYGDASVLDEARTALSDVAGVEEVL
HHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
ANLKSIAAHLEGRTVSFDLADLRGYSYYSGARFTIYARNATDAVVRGGRYDEVGAAFGRT
HHHHHHHHHHCCCEEEEEHHHCCCCCEECCCEEEEEECCCCHHHHCCCCCHHHHHHHCCC
RPAAGFSLDIKQLVGIVPPRTLKAAIRAPWAATPDAHRFIAQLRSAGETVVCVLPGHESQ
CCCCCCCCCHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEEEECCCHHH
VQEFQCDRELVLEQGQWMVRPLPY
HHHHHCCHHHHHHCCCEEECCCCC
>Mature Secondary Structure 
SAWVLPDHIADVLPSEARHIEELRRGLIDTARGYGYELVMPPMLEHLESLLSGTGEALD
CCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHCCCCCEEH
LQTFKLVDQLSGRSIGLRADMTQQVARIDAHLLNRSGVTRLCYCGPVLHTRPDRPRATRE
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCHHCCCCCCCCCHHH
PLQFGAEIYGHSGMEADLESVLLALDCLHLAGVEGVSVDLSDARVVRTLLEPIAADSATV
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCEEECHHHHHHHHHHHHHCCCHHHH
RRIYAALAAKDASELSQASAGLPSGTRRALSAVLELYGDASVLDEARTALSDVAGVEEVL
HHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
ANLKSIAAHLEGRTVSFDLADLRGYSYYSGARFTIYARNATDAVVRGGRYDEVGAAFGRT
HHHHHHHHHHCCCEEEEEHHHCCCCCEECCCEEEEEECCCCHHHHCCCCCHHHHHHHCCC
RPAAGFSLDIKQLVGIVPPRTLKAAIRAPWAATPDAHRFIAQLRSAGETVVCVLPGHESQ
CCCCCCCCCHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEEEECCCHHH
VQEFQCDRELVLEQGQWMVRPLPY
HHHHHCCHHHHHHCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+ [C]

Kcat value (1/min): 8520 [C]

Specific activity: 121.8

Km value (mM): 0.06 {His}} 0.046 {His}} 0.03 {His}} 0.02 {His}} 0.017 {His}} 0.011 {His}} 0.008 {His}} 0.0014 {tRNAHis}} 0.00056 {tRNAHis}} 0.0016 {U73tRNAHisGUG}} 0.00031 {U73tRNAHisGUG}} 0.89 {ATP}} 0.74 {ATP}} 0.655 {ATP}} 0.5

Substrates: ATP; @HIST01.txt*L-histidine!; tRNAHis [C]

Specific reaction: ATP + @HIST01.txt*L-histidine! + tRNAHis = AMP + diphosphate +L-histidyl-tRNAHis [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 1, 2, 4-Triazole-3-alanine; D-Histidine; Diphosphate; L-Histidinol; Mg2+; N-Acetyl histidine; NEM; p-Chloromercuribenzoate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA