BacMap

Definition	Acidovorax citrulli AAC00-1 chromosome, complete genome.
Accession	NC_008752
Length	5,352,772

Click here to switch to the map view.

The map label for this gene is braG [H]

Identifier: 120609968

GI number: 120609968

Start: 1406617

End: 1407330

Strand: Direct

Name: braG [H]

Synonym: Aave_1281

Alternate gene names: 120609968

Gene position: 1406617-1407330 (Clockwise)

Preceding gene: 120609967

Following gene: 120609969

Centisome position: 26.28

GC content: 69.61

Gene sequence:

>714_bases
ATGGCATCGTCCCCCGCTCTCGAAATCCGCGGCCTGGAGGCCTGGTACGGCGAATCGCACGTGCTGCACGGCGTGGACAT
GGTCGTGCAGCCCGGCGAGGTGGTCACGCTGCTGGGCCGCAACGGCGCGGGCCGCACCTCCACGCTGCGCGCGGTGATGG
GCCTGACCGGCTCGCGCAAGGGCTCGGTGAAGATCAACGGGCAGGAAACCATCCAGATGCCCACGCACCGCATCGCGCAC
CTGGGCGTGGGCTACTGCCCCGAGGAGCGCGGCATCTTCTCCAGCCTGTCGTGCGAGGAGAACCTGCTGCTGCCGCCCGT
GCTCAAGACGGGCCGCGCCGGCATGCCGCTGGACGAGATCTATGCGATGTTCCCCAATCTCGCGGAGCGCCGCCACAGCC
AGGGCACGCGCCTGTCGGGCGGGGAGCAGCAGATGCTGGCCGTGGCGCGCATCCTGCGCACGGGCGCGCAGCTGCTGCTG
CTCGACGAGATCTCCGAAGGGCTCGCGCCCGTCATCGTGCAGGCCCTGGCGCGCATGATCACCACGCTGCGCCAGAAGGG
CTACACGGTGGTGATGGTGGAGCAGAACTTCCGCTTCGCCGCGCCGCTCGCCGACCGCTTCTACGTGATGGAGCACGGCA
GCATCGCCCTGCAGTTCGGCGCCGCCGAGCTGGAGCAGCGCATGCCCGTGCTGAACGAATTGCTGGGCGTGTGA

Upstream 100 bases:

>100_bases
ACGGCGTCTGCTGGCCTGGCCTGCTCCGCGGCCTTCGGAGCGGAAAGGCCGTGCTGCTTTCAACGGCGGCTGGCCGCAAT
CTGCGCATTGGATCACTGAG

Downstream 100 bases:

>100_bases
GCCGCCGGCCTTTCGCCCGCCCGTGACGCGCCGAACCCGAACCGACCGAACGAACGACCACCCAGCCTCAGGAGACGACG
ACATGAAGAAACACCCACGC

Product: ABC transporter-like protein

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 237; Mature: 236

Protein sequence:

>237_residues
MASSPALEIRGLEAWYGESHVLHGVDMVVQPGEVVTLLGRNGAGRTSTLRAVMGLTGSRKGSVKINGQETIQMPTHRIAH
LGVGYCPEERGIFSSLSCEENLLLPPVLKTGRAGMPLDEIYAMFPNLAERRHSQGTRLSGGEQQMLAVARILRTGAQLLL
LDEISEGLAPVIVQALARMITTLRQKGYTVVMVEQNFRFAAPLADRFYVMEHGSIALQFGAAELEQRMPVLNELLGV

Sequences:

>Translated_237_residues
MASSPALEIRGLEAWYGESHVLHGVDMVVQPGEVVTLLGRNGAGRTSTLRAVMGLTGSRKGSVKINGQETIQMPTHRIAH
LGVGYCPEERGIFSSLSCEENLLLPPVLKTGRAGMPLDEIYAMFPNLAERRHSQGTRLSGGEQQMLAVARILRTGAQLLL
LDEISEGLAPVIVQALARMITTLRQKGYTVVMVEQNFRFAAPLADRFYVMEHGSIALQFGAAELEQRMPVLNELLGV
>Mature_236_residues
ASSPALEIRGLEAWYGESHVLHGVDMVVQPGEVVTLLGRNGAGRTSTLRAVMGLTGSRKGSVKINGQETIQMPTHRIAHL
GVGYCPEERGIFSSLSCEENLLLPPVLKTGRAGMPLDEIYAMFPNLAERRHSQGTRLSGGEQQMLAVARILRTGAQLLLL
DEISEGLAPVIVQALARMITTLRQKGYTVVMVEQNFRFAAPLADRFYVMEHGSIALQFGAAELEQRMPVLNELLGV

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0410

COG function: function code E; ABC-type branched-chain amino acid transport systems, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI45446740, Length=196, Percent_Identity=29.5918367346939, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI47078218, Length=196, Percent_Identity=29.5918367346939, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI21536376, Length=200, Percent_Identity=28.5, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI27881501, Length=214, Percent_Identity=25.7009345794392, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI30795238, Length=214, Percent_Identity=25.7009345794392, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI31657092, Length=224, Percent_Identity=24.5535714285714, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI27436953, Length=195, Percent_Identity=28.7179487179487, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI150417984, Length=217, Percent_Identity=29.0322580645161, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI105990541, Length=193, Percent_Identity=26.9430051813472, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI116734710, Length=200, Percent_Identity=29.5, Blast_Score=72, Evalue=3e-13,
Organism=Homo sapiens, GI42741659, Length=196, Percent_Identity=26.530612244898, Blast_Score=68, Evalue=7e-12,
Organism=Homo sapiens, GI9961250, Length=230, Percent_Identity=25.2173913043478, Blast_Score=68, Evalue=8e-12,
Organism=Homo sapiens, GI4505771, Length=230, Percent_Identity=25.2173913043478, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI9961252, Length=161, Percent_Identity=27.9503105590062, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI153792144, Length=198, Percent_Identity=25.2525252525253, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI27262624, Length=216, Percent_Identity=25, Blast_Score=65, Evalue=4e-11,
Organism=Homo sapiens, GI27262626, Length=216, Percent_Identity=25, Blast_Score=65, Evalue=4e-11,
Organism=Escherichia coli, GI87082268, Length=212, Percent_Identity=39.1509433962264, Blast_Score=147, Evalue=6e-37,
Organism=Escherichia coli, GI1789593, Length=238, Percent_Identity=31.0924369747899, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1789672, Length=223, Percent_Identity=28.2511210762332, Blast_Score=101, Evalue=4e-23,
Organism=Escherichia coli, GI1788225, Length=232, Percent_Identity=32.7586206896552, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787029, Length=216, Percent_Identity=28.7037037037037, Blast_Score=95, Evalue=3e-21,
Organism=Escherichia coli, GI1787792, Length=224, Percent_Identity=29.9107142857143, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI1789864, Length=235, Percent_Identity=28.0851063829787, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI1788450, Length=219, Percent_Identity=30.1369863013699, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1790525, Length=221, Percent_Identity=26.6968325791855, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI48995001, Length=224, Percent_Identity=30.8035714285714, Blast_Score=87, Evalue=8e-19,
Organism=Escherichia coli, GI1788897, Length=232, Percent_Identity=27.1551724137931, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1787089, Length=228, Percent_Identity=28.9473684210526, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI1788761, Length=220, Percent_Identity=27.7272727272727, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1786253, Length=231, Percent_Identity=30.3030303030303, Blast_Score=77, Evalue=9e-16,
Organism=Escherichia coli, GI1787370, Length=220, Percent_Identity=27.2727272727273, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI87081791, Length=226, Percent_Identity=26.9911504424779, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1790190, Length=226, Percent_Identity=23.4513274336283, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1788165, Length=215, Percent_Identity=28.8372093023256, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1787105, Length=222, Percent_Identity=25.6756756756757, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1786703, Length=225, Percent_Identity=28, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI87081709, Length=234, Percent_Identity=28.2051282051282, Blast_Score=71, Evalue=5e-14,
Organism=Escherichia coli, GI1787712, Length=215, Percent_Identity=26.046511627907, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI1786398, Length=212, Percent_Identity=25, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI1786872, Length=214, Percent_Identity=28.5046728971963, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1788644, Length=232, Percent_Identity=28.0172413793103, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788210, Length=228, Percent_Identity=25.8771929824561, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1789991, Length=236, Percent_Identity=26.6949152542373, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1786319, Length=217, Percent_Identity=26.2672811059908, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1789873, Length=212, Percent_Identity=22.1698113207547, Blast_Score=68, Evalue=4e-13,
Organism=Escherichia coli, GI87081782, Length=206, Percent_Identity=28.6407766990291, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1788472, Length=219, Percent_Identity=24.6575342465753, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI48994943, Length=216, Percent_Identity=24.537037037037, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787112, Length=238, Percent_Identity=28.9915966386555, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17510237, Length=232, Percent_Identity=29.7413793103448, Blast_Score=84, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI115533608, Length=227, Percent_Identity=28.6343612334802, Blast_Score=81, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI71996809, Length=225, Percent_Identity=25.7777777777778, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI193211015, Length=225, Percent_Identity=25.7777777777778, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI193211017, Length=225, Percent_Identity=25.7777777777778, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71983510, Length=207, Percent_Identity=25.1207729468599, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71983516, Length=207, Percent_Identity=25.1207729468599, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71983523, Length=207, Percent_Identity=25.1207729468599, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17565586, Length=216, Percent_Identity=26.8518518518519, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24666092, Length=212, Percent_Identity=29.7169811320755, Blast_Score=94, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24648314, Length=226, Percent_Identity=27.4336283185841, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI221512771, Length=230, Percent_Identity=24.3478260869565, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24643674, Length=217, Percent_Identity=26.7281105990783, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI28573571, Length=229, Percent_Identity=24.4541484716157, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI28573573, Length=229, Percent_Identity=24.4541484716157, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI28574150, Length=217, Percent_Identity=26.2672811059908, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI116007328, Length=217, Percent_Identity=26.2672811059908, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI85725262, Length=201, Percent_Identity=26.3681592039801, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI45550390, Length=223, Percent_Identity=24.6636771300448, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 25804; Mature: 25673

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: PS00211 ABC_TRANSPORTER_1 ; PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASSPALEIRGLEAWYGESHVLHGVDMVVQPGEVVTLLGRNGAGRTSTLRAVMGLTGSRK
CCCCCCEEEECCHHHCCCCEEEECCCEEECCCCEEEEEECCCCCHHHHHHHHHHCCCCCC
GSVKINGQETIQMPTHRIAHLGVGYCPEERGIFSSLSCEENLLLPPVLKTGRAGMPLDEI
CCEEECCCCEEECCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCHHHH
YAMFPNLAERRHSQGTRLSGGEQQMLAVARILRTGAQLLLLDEISEGLAPVIVQALARMI
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEHHHHHCCHHHHHHHHHHHHH
TTLRQKGYTVVMVEQNFRFAAPLADRFYVMEHGSIALQFGAAELEQRMPVLNELLGV
HHHHHCCCEEEEEECCCEEECCCCCEEEEEECCCEEEEECHHHHHHHCHHHHHHHCC
>Mature Secondary Structure 
ASSPALEIRGLEAWYGESHVLHGVDMVVQPGEVVTLLGRNGAGRTSTLRAVMGLTGSRK
CCCCCEEEECCHHHCCCCEEEECCCEEECCCCEEEEEECCCCCHHHHHHHHHHCCCCCC
GSVKINGQETIQMPTHRIAHLGVGYCPEERGIFSSLSCEENLLLPPVLKTGRAGMPLDEI
CCEEECCCCEEECCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCHHHH
YAMFPNLAERRHSQGTRLSGGEQQMLAVARILRTGAQLLLLDEISEGLAPVIVQALARMI
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEHHHHHCCHHHHHHHHHHHHH
TTLRQKGYTVVMVEQNFRFAAPLADRFYVMEHGSIALQFGAAELEQRMPVLNELLGV
HHHHHCCCEEEEEECCCEEECCCCCEEEEEECCCEEEEECHHHHHHHCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]