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The map label for this gene is asnB [H]

Identifier: 120609653

GI number: 120609653

Start: 1046576

End: 1048483

Strand: Direct

Name: asnB [H]

Synonym: Aave_0959

Alternate gene names: 120609653

Gene position: 1046576-1048483 (Clockwise)

Preceding gene: 120609652

Following gene: 120609654

Centisome position: 19.55

GC content: 53.98

Gene sequence:

>1908_bases
ATGTGTGGAATTTTTGGCCTGATTGGCTGGCCCAATGTAAGCGAGGCGGTGGAGCGTGTCTCCCGTGCCATGGATACCAT
GGTCCATCGCGGACCTGACGGAGACGGACTTTACGTCCACGAAGAGGGCGGGGCCGTTTTCGGGCACCGACGGCTCGCGA
TTATCGATCCGGCCAACGGCAAACAGCCGATGACCACGTCGGATGGTAGATTCACGGTTGTTTTCAACGGTGCAATATAT
AATTATCTAGAACTCCGTCGAGAGCTGGTCGCGAAGGGGCATGCCATTGCCTCTTACTCAGATACCGAGGTGCTGCTGGC
CGCCTACAGGGAATGGGGCGAACAGTGTGTCGACCGTTTTCAAGGCATGTTTGCATTCGCCATCTGGGATCTTTCTCGCC
GAGAGGTGTTCTGCGCCCGGGACCGCGTAGGCATAAAGCCGTTCTACTATATCGATAGCGATGAATTCTTCTCTCTCTCA
TCGGAGATCAAGGCGTTTGTCGGGGCGGGATTGCTGGACGTTTCCTTGAACCAGGCTGCGCTGAAAGATTATCTCGCCTT
TCAGTTCTGTCTGGGCGACAAGACACTCTTTTCGGGCGTGAAGAAGCTCGAGCCTGGATTCTGCCTGAAAATCAGCGTCC
GGGACGGGCGCCGAGAGGTGGTCAAGAGGCAATATTGGGATGTCACTTATGATATCGACCAGGTCCACGACGAGTCCTGG
TTCGTCGATCAACTTGCTGCCTTGATCGAGGATTCTACGCGGATGCATATTCGCTCCGATGTGCCTTTGGGGGCGCATCT
TTCCGGCGGGCTGGACTCGAGCACAGTCGTCTGCTTGGTGGCGAAGATGCTGCAGGGCGAAAGCCTTCATACCTTCACCG
GTACATTTCCCGATGGGCCGCAGTATGACGAGACAGAGCATGCCAAGGCGGTTTCCGCTTTTGCCGGCACGAACTATCAC
GAAATATCCATTCAGGGAGCAGATTTCTCCACTCTGTTCCCCAAATTGATGTATTTCATGGATGAGCCTGCCGCCGGGCC
GGGACTCATTCCACAGTACCATGTTTCGAAACTGGCCTCCCAGCAGGTGAAAGTCGTGCTGGGTGGGCAAGGTGGAGACG
AGCTCTTCATCGGATATGCCCGGTATCTGGTGGCGTATTTGGAGGCCTGCCTGCAGGGCGCGATCGATGAAAGTTTCCGC
GACGGTCCGTATGCTGTCACATTGGAATCCATCGTTCCGAACCTGCCAGTATTGAAGGCTTACAAGCCCATGCTGCGGAA
AAACTGGTCGCAAGGTCTTTTCGGTCCGGCGGCGGAGCGTTATTTTCAGTTGATCGACCGTGCGGAAGAGTCTGTAGGAT
TTTACTCCGCAGACGTCTTTGGTGGCGCGTACTCTGCTTACGACGAGTTCAATGCCATTTTCAATCGACCAGGTGTCGAT
TCGCTCATTAATAAAATGACCTATTTCGACCTGAAGGCGTCCTTGCCAGCCTTGCTTCATGTCGAAGATAGAACGAGCAT
GTCTGCTTCGATCGAGTCGCGGGTTCCCTTGCTTGATCATAGAATTGTCGAATTCATGGCGAGAATACCGCCCAATAAGA
AATTCGCTGGAGGCCGGCTCAAGCATCTTTTCAAGTCGGCCACGAGAAACATCGTTCCCAAGAGTGTCATGCAGCGCAAG
GATAAAATGGGGTTCCCGACTCCGTTGAATAATTGGACGCAGAGTGTTGCCAAGGAATATGTTGATGATTTGATTTTTTC
TCGAAAAACCAGGGAGCGCGGAATATTCAATGTCGACCAGATCGACGCAGGCCAGGGCAGGCAGGGCGAATATGGCCGGG
CGCTTTGGGGCATGATGTGCCTGGAGCAGTGGCATCGTAGTTTCAAGGACGGTGATGCCTTCCGGTGA

Upstream 100 bases:

>100_bases
AAGGAAGGCGCCGAATTGGCAGGAAGGGAACTGAACTGGGATGTGGAAGGCCGGAAGCTGATGGATATATACGCATCCCT
GTAAGGCGTGAGGGTTTAAT

Downstream 100 bases:

>100_bases
TCGCAATCCCGATAAGGTTTTGGTGCTCACTGCCGATCACGAAGTGGATCGCAGAATATTGCAGCAGATATCCCTGCTGG
AAAGCGCTGGCTGGGATGTC

Product: asparagine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 635; Mature: 635

Protein sequence:

>635_residues
MCGIFGLIGWPNVSEAVERVSRAMDTMVHRGPDGDGLYVHEEGGAVFGHRRLAIIDPANGKQPMTTSDGRFTVVFNGAIY
NYLELRRELVAKGHAIASYSDTEVLLAAYREWGEQCVDRFQGMFAFAIWDLSRREVFCARDRVGIKPFYYIDSDEFFSLS
SEIKAFVGAGLLDVSLNQAALKDYLAFQFCLGDKTLFSGVKKLEPGFCLKISVRDGRREVVKRQYWDVTYDIDQVHDESW
FVDQLAALIEDSTRMHIRSDVPLGAHLSGGLDSSTVVCLVAKMLQGESLHTFTGTFPDGPQYDETEHAKAVSAFAGTNYH
EISIQGADFSTLFPKLMYFMDEPAAGPGLIPQYHVSKLASQQVKVVLGGQGGDELFIGYARYLVAYLEACLQGAIDESFR
DGPYAVTLESIVPNLPVLKAYKPMLRKNWSQGLFGPAAERYFQLIDRAEESVGFYSADVFGGAYSAYDEFNAIFNRPGVD
SLINKMTYFDLKASLPALLHVEDRTSMSASIESRVPLLDHRIVEFMARIPPNKKFAGGRLKHLFKSATRNIVPKSVMQRK
DKMGFPTPLNNWTQSVAKEYVDDLIFSRKTRERGIFNVDQIDAGQGRQGEYGRALWGMMCLEQWHRSFKDGDAFR

Sequences:

>Translated_635_residues
MCGIFGLIGWPNVSEAVERVSRAMDTMVHRGPDGDGLYVHEEGGAVFGHRRLAIIDPANGKQPMTTSDGRFTVVFNGAIY
NYLELRRELVAKGHAIASYSDTEVLLAAYREWGEQCVDRFQGMFAFAIWDLSRREVFCARDRVGIKPFYYIDSDEFFSLS
SEIKAFVGAGLLDVSLNQAALKDYLAFQFCLGDKTLFSGVKKLEPGFCLKISVRDGRREVVKRQYWDVTYDIDQVHDESW
FVDQLAALIEDSTRMHIRSDVPLGAHLSGGLDSSTVVCLVAKMLQGESLHTFTGTFPDGPQYDETEHAKAVSAFAGTNYH
EISIQGADFSTLFPKLMYFMDEPAAGPGLIPQYHVSKLASQQVKVVLGGQGGDELFIGYARYLVAYLEACLQGAIDESFR
DGPYAVTLESIVPNLPVLKAYKPMLRKNWSQGLFGPAAERYFQLIDRAEESVGFYSADVFGGAYSAYDEFNAIFNRPGVD
SLINKMTYFDLKASLPALLHVEDRTSMSASIESRVPLLDHRIVEFMARIPPNKKFAGGRLKHLFKSATRNIVPKSVMQRK
DKMGFPTPLNNWTQSVAKEYVDDLIFSRKTRERGIFNVDQIDAGQGRQGEYGRALWGMMCLEQWHRSFKDGDAFR
>Mature_635_residues
MCGIFGLIGWPNVSEAVERVSRAMDTMVHRGPDGDGLYVHEEGGAVFGHRRLAIIDPANGKQPMTTSDGRFTVVFNGAIY
NYLELRRELVAKGHAIASYSDTEVLLAAYREWGEQCVDRFQGMFAFAIWDLSRREVFCARDRVGIKPFYYIDSDEFFSLS
SEIKAFVGAGLLDVSLNQAALKDYLAFQFCLGDKTLFSGVKKLEPGFCLKISVRDGRREVVKRQYWDVTYDIDQVHDESW
FVDQLAALIEDSTRMHIRSDVPLGAHLSGGLDSSTVVCLVAKMLQGESLHTFTGTFPDGPQYDETEHAKAVSAFAGTNYH
EISIQGADFSTLFPKLMYFMDEPAAGPGLIPQYHVSKLASQQVKVVLGGQGGDELFIGYARYLVAYLEACLQGAIDESFR
DGPYAVTLESIVPNLPVLKAYKPMLRKNWSQGLFGPAAERYFQLIDRAEESVGFYSADVFGGAYSAYDEFNAIFNRPGVD
SLINKMTYFDLKASLPALLHVEDRTSMSASIESRVPLLDHRIVEFMARIPPNKKFAGGRLKHLFKSATRNIVPKSVMQRK
DKMGFPTPLNNWTQSVAKEYVDDLIFSRKTRERGIFNVDQIDAGQGRQGEYGRALWGMMCLEQWHRSFKDGDAFR

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=416, Percent_Identity=27.1634615384615, Blast_Score=105, Evalue=2e-22,
Organism=Homo sapiens, GI168229252, Length=416, Percent_Identity=27.1634615384615, Blast_Score=105, Evalue=2e-22,
Organism=Homo sapiens, GI168229250, Length=416, Percent_Identity=27.1634615384615, Blast_Score=105, Evalue=2e-22,
Organism=Homo sapiens, GI296010848, Length=390, Percent_Identity=26.4102564102564, Blast_Score=99, Evalue=9e-21,
Organism=Escherichia coli, GI1786889, Length=399, Percent_Identity=30.5764411027569, Blast_Score=150, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI71993933, Length=391, Percent_Identity=26.5984654731458, Blast_Score=112, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI25147557, Length=391, Percent_Identity=26.5984654731458, Blast_Score=112, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI17560178, Length=395, Percent_Identity=26.5822784810127, Blast_Score=105, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI25147560, Length=359, Percent_Identity=27.5766016713092, Blast_Score=96, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6325403, Length=419, Percent_Identity=26.2529832935561, Blast_Score=122, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6321563, Length=415, Percent_Identity=25.3012048192771, Blast_Score=121, Evalue=3e-28,
Organism=Drosophila melanogaster, GI45553209, Length=398, Percent_Identity=28.894472361809, Blast_Score=150, Evalue=4e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 71204; Mature: 71204

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIFGLIGWPNVSEAVERVSRAMDTMVHRGPDGDGLYVHEEGGAVFGHRRLAIIDPANG
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEECCCEEEEECCCCC
KQPMTTSDGRFTVVFNGAIYNYLELRRELVAKGHAIASYSDTEVLLAAYREWGEQCVDRF
CCCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHH
QGMFAFAIWDLSRREVFCARDRVGIKPFYYIDSDEFFSLSSEIKAFVGAGLLDVSLNQAA
HHHHHHHHHHCCCCCEEECCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCHHEECCCHHH
LKDYLAFQFCLGDKTLFSGVKKLEPGFCLKISVRDGRREVVKRQYWDVTYDIDQVHDESW
HHHHHHHHHHCCCHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHCCEEEEHHHHCCCHH
FVDQLAALIEDSTRMHIRSDVPLGAHLSGGLDSSTVVCLVAKMLQGESLHTFTGTFPDGP
HHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCC
QYDETEHAKAVSAFAGTNYHEISIQGADFSTLFPKLMYFMDEPAAGPGLIPQYHVSKLAS
CCCCHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHC
QQVKVVLGGQGGDELFIGYARYLVAYLEACLQGAIDESFRDGPYAVTLESIVPNLPVLKA
CCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHCCCCCHHHH
YKPMLRKNWSQGLFGPAAERYFQLIDRAEESVGFYSADVFGGAYSAYDEFNAIFNRPGVD
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEHHHCCCHHHHHHHHHHHHCCCCHH
SLINKMTYFDLKASLPALLHVEDRTSMSASIESRVPLLDHRIVEFMARIPPNKKFAGGRL
HHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHH
KHLFKSATRNIVPKSVMQRKDKMGFPTPLNNWTQSVAKEYVDDLIFSRKTRERGIFNVDQ
HHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
IDAGQGRQGEYGRALWGMMCLEQWHRSFKDGDAFR
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MCGIFGLIGWPNVSEAVERVSRAMDTMVHRGPDGDGLYVHEEGGAVFGHRRLAIIDPANG
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEECCCEEEEECCCCC
KQPMTTSDGRFTVVFNGAIYNYLELRRELVAKGHAIASYSDTEVLLAAYREWGEQCVDRF
CCCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHH
QGMFAFAIWDLSRREVFCARDRVGIKPFYYIDSDEFFSLSSEIKAFVGAGLLDVSLNQAA
HHHHHHHHHHCCCCCEEECCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCHHEECCCHHH
LKDYLAFQFCLGDKTLFSGVKKLEPGFCLKISVRDGRREVVKRQYWDVTYDIDQVHDESW
HHHHHHHHHHCCCHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHCCEEEEHHHHCCCHH
FVDQLAALIEDSTRMHIRSDVPLGAHLSGGLDSSTVVCLVAKMLQGESLHTFTGTFPDGP
HHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCC
QYDETEHAKAVSAFAGTNYHEISIQGADFSTLFPKLMYFMDEPAAGPGLIPQYHVSKLAS
CCCCHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHC
QQVKVVLGGQGGDELFIGYARYLVAYLEACLQGAIDESFRDGPYAVTLESIVPNLPVLKA
CCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHCCCCCHHHH
YKPMLRKNWSQGLFGPAAERYFQLIDRAEESVGFYSADVFGGAYSAYDEFNAIFNRPGVD
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEHHHCCCHHHHHHHHHHHHCCCCHH
SLINKMTYFDLKASLPALLHVEDRTSMSASIESRVPLLDHRIVEFMARIPPNKKFAGGRL
HHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHH
KHLFKSATRNIVPKSVMQRKDKMGFPTPLNNWTQSVAKEYVDDLIFSRKTRERGIFNVDQ
HHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
IDAGQGRQGEYGRALWGMMCLEQWHRSFKDGDAFR
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]