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The map label for this gene is pulE [H]

Identifier: 120609610

GI number: 120609610

Start: 997913

End: 999274

Strand: Reverse

Name: pulE [H]

Synonym: Aave_0916

Alternate gene names: 120609610

Gene position: 999274-997913 (Counterclockwise)

Preceding gene: 120609611

Following gene: 120609607

Centisome position: 18.67

GC content: 70.12

Gene sequence:

>1362_bases
ATGCGCCACCCGCTTCCCTACGCCTTCGCGCGCAGTTCGCAACTGCTGCTGGAGGACGACGGCCACACCTGCGTGCTCTG
GCACGGCCCCGCACCGGATGCCGGGGCACTGTCCGAGGTCATGCGCAAGCATGCCGTGCGGGAGCTGATGCCGCTGGACG
CGCACGCCATCGCGCAGCGCATCAGCGCGGCCTATTCCCAAGGGGAATCCAGCGCGGCGACGGTGGTGAGCGAGGTCGAA
TCCGACGCCGACCTCTCGCGCATGATGCAGGAGCTGCCGGCCGTGGAGGACCTGCTGGAATCGGCGGGCGACGCGCCCAT
CATCCGCATGCTCAACGCCCTGCTCACGCAGGCCGCGCGCGACGGCGCGAGCGACATCCACATCGAGCCCTACGAGCGGC
ATTCGAGCGTGCGCTTCCGCGTGGACGGCACGCTGCGCGAAGTGGTGCAGCCGAACCGCGCGCTGCATGCGGCGCTGATC
TCCCGCCTGAAGATCATGGCGGACCTGGACATCTCCGAAAAGCGCCTGCCCCAGGACGGCCGCATCAGCCTGCGGCTGGG
CACGCGCGCGATCGACGTGCGCGTCTCGACCCTGCCGAGCGCCCATGGCGAGCGGGCGGTGCTGCGCCTGCTGGACAAGA
GCGAGAGCAAGCTGAGCCTGGAGGCCGTGGGCATGCAGGGCGACACGCTGCGCCGCTTCGACGGGCTGATCCACCAGCCG
CATGGCATCGTGCTCGTGACAGGGCCCACGGGCTCGGGCAAGACCACCACGCTGTATGCGGCGCTGGGCCGGCTCGACGC
CACCAGCAGCAACATCATGACGGTGGAGGACCCGATCGAGTACGAACTGCCGGGCGTGGGCCAGACCCAGGTCAACAGCA
AGATCGACCTCACCTTCGCCAAGGCGCTGCGCGCCATCCTGCGCCAGGACCCGGACGTGATCATGATCGGCGAGATCCGC
GATTTCGAGACCGCGCAGATCGCCATCCAGGCCTCGCTGACGGGCCACCTGGTGCTGGCCACGCTGCACACCAACGACGC
GGCCAGCGCCGTCACCCGCCTGACCGACATGGGCGTGGAGCCGTTCCTGCTCTCCAGCTCCCTGCTGGGCGTGCTGGCGC
AGCGGCTGGTGCGCAAGCTCGATCCGACCGAGCCCAGTGGCTACCGCGGCCGCACGGGCGTGTTCGAGCTCCTGGTGGTG
GACGACACGCTGCGCGCGCAGATCCACGGCCAGGCGGCCGAGGCGGAACTGCGCGCCACCGCGATCGCCGGCGGCATGAC
GCTCATGCGCGAGGACGGTGAGCGGCTGGTGCGTGCCGGCATCACGAGCCCCGAGGAAGTGCTGCGCGTCACGCGCGAAT
AG

Upstream 100 bases:

>100_bases
GCGCCGGCACGGGCCCCCGCACGCGCCCCGGCGCCGGCCGATGCCGGGGCCTACGACCCGCAGTAACCGGACATTCCCGT
CCTCCCACTCCCCCCACGCC

Downstream 100 bases:

>100_bases
CGCGGCTTCCGGCGCGCCCGCCGCTCAGCCGGCCGGCAGCAGTTCCAGCACCGTCTCCGGCGGCCGGCACAGGCGCGCGC
CGCGCGGGGTGACCACGATG

Product: general secretory pathway protein E

Products: NA

Alternate protein names: Pullulanase secretion protein pulE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MRHPLPYAFARSSQLLLEDDGHTCVLWHGPAPDAGALSEVMRKHAVRELMPLDAHAIAQRISAAYSQGESSAATVVSEVE
SDADLSRMMQELPAVEDLLESAGDAPIIRMLNALLTQAARDGASDIHIEPYERHSSVRFRVDGTLREVVQPNRALHAALI
SRLKIMADLDISEKRLPQDGRISLRLGTRAIDVRVSTLPSAHGERAVLRLLDKSESKLSLEAVGMQGDTLRRFDGLIHQP
HGIVLVTGPTGSGKTTTLYAALGRLDATSSNIMTVEDPIEYELPGVGQTQVNSKIDLTFAKALRAILRQDPDVIMIGEIR
DFETAQIAIQASLTGHLVLATLHTNDAASAVTRLTDMGVEPFLLSSSLLGVLAQRLVRKLDPTEPSGYRGRTGVFELLVV
DDTLRAQIHGQAAEAELRATAIAGGMTLMREDGERLVRAGITSPEEVLRVTRE

Sequences:

>Translated_453_residues
MRHPLPYAFARSSQLLLEDDGHTCVLWHGPAPDAGALSEVMRKHAVRELMPLDAHAIAQRISAAYSQGESSAATVVSEVE
SDADLSRMMQELPAVEDLLESAGDAPIIRMLNALLTQAARDGASDIHIEPYERHSSVRFRVDGTLREVVQPNRALHAALI
SRLKIMADLDISEKRLPQDGRISLRLGTRAIDVRVSTLPSAHGERAVLRLLDKSESKLSLEAVGMQGDTLRRFDGLIHQP
HGIVLVTGPTGSGKTTTLYAALGRLDATSSNIMTVEDPIEYELPGVGQTQVNSKIDLTFAKALRAILRQDPDVIMIGEIR
DFETAQIAIQASLTGHLVLATLHTNDAASAVTRLTDMGVEPFLLSSSLLGVLAQRLVRKLDPTEPSGYRGRTGVFELLVV
DDTLRAQIHGQAAEAELRATAIAGGMTLMREDGERLVRAGITSPEEVLRVTRE
>Mature_453_residues
MRHPLPYAFARSSQLLLEDDGHTCVLWHGPAPDAGALSEVMRKHAVRELMPLDAHAIAQRISAAYSQGESSAATVVSEVE
SDADLSRMMQELPAVEDLLESAGDAPIIRMLNALLTQAARDGASDIHIEPYERHSSVRFRVDGTLREVVQPNRALHAALI
SRLKIMADLDISEKRLPQDGRISLRLGTRAIDVRVSTLPSAHGERAVLRLLDKSESKLSLEAVGMQGDTLRRFDGLIHQP
HGIVLVTGPTGSGKTTTLYAALGRLDATSSNIMTVEDPIEYELPGVGQTQVNSKIDLTFAKALRAILRQDPDVIMIGEIR
DFETAQIAIQASLTGHLVLATLHTNDAASAVTRLTDMGVEPFLLSSSLLGVLAQRLVRKLDPTEPSGYRGRTGVFELLVV
DDTLRAQIHGQAAEAELRATAIAGGMTLMREDGERLVRAGITSPEEVLRVTRE

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of pullulanase [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=425, Percent_Identity=53.1764705882353, Blast_Score=436, Evalue=1e-123,
Organism=Escherichia coli, GI1786296, Length=410, Percent_Identity=42.6829268292683, Blast_Score=292, Evalue=3e-80,
Organism=Escherichia coli, GI87082188, Length=175, Percent_Identity=45.7142857142857, Blast_Score=124, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 49155; Mature: 49155

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: PS00211 ABC_TRANSPORTER_1 ; PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHPLPYAFARSSQLLLEDDGHTCVLWHGPAPDAGALSEVMRKHAVRELMPLDAHAIAQR
CCCCCCCHHCCCCEEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHH
ISAAYSQGESSAATVVSEVESDADLSRMMQELPAVEDLLESAGDAPIIRMLNALLTQAAR
HHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHH
DGASDIHIEPYERHSSVRFRVDGTLREVVQPNRALHAALISRLKIMADLDISEKRLPQDG
CCCCCCCCCCCCCCCCEEEEECCHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHCCCCC
RISLRLGTRAIDVRVSTLPSAHGERAVLRLLDKSESKLSLEAVGMQGDTLRRFDGLIHQP
EEEEEECCEEEEEEEECCCCCCCHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHCCC
HGIVLVTGPTGSGKTTTLYAALGRLDATSSNIMTVEDPIEYELPGVGQTQVNSKIDLTFA
CCEEEEECCCCCCCCEEHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCEEEHHHH
KALRAILRQDPDVIMIGEIRDFETAQIAIQASLTGHLVLATLHTNDAASAVTRLTDMGVE
HHHHHHHHCCCCEEEEECCCCCCEEEEEEEEECCCEEEEEEEECCHHHHHHHHHHHCCCC
PFLLSSSLLGVLAQRLVRKLDPTEPSGYRGRTGVFELLVVDDTLRAQIHGQAAEAELRAT
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCHHHHHHCCCHHHHHHHHH
AIAGGMTLMREDGERLVRAGITSPEEVLRVTRE
HHHCCHHHHHHHHHHHHHHCCCCHHHHHHHCCC
>Mature Secondary Structure
MRHPLPYAFARSSQLLLEDDGHTCVLWHGPAPDAGALSEVMRKHAVRELMPLDAHAIAQR
CCCCCCCHHCCCCEEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHH
ISAAYSQGESSAATVVSEVESDADLSRMMQELPAVEDLLESAGDAPIIRMLNALLTQAAR
HHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHH
DGASDIHIEPYERHSSVRFRVDGTLREVVQPNRALHAALISRLKIMADLDISEKRLPQDG
CCCCCCCCCCCCCCCCEEEEECCHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHCCCCC
RISLRLGTRAIDVRVSTLPSAHGERAVLRLLDKSESKLSLEAVGMQGDTLRRFDGLIHQP
EEEEEECCEEEEEEEECCCCCCCHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHCCC
HGIVLVTGPTGSGKTTTLYAALGRLDATSSNIMTVEDPIEYELPGVGQTQVNSKIDLTFA
CCEEEEECCCCCCCCEEHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCEEEHHHH
KALRAILRQDPDVIMIGEIRDFETAQIAIQASLTGHLVLATLHTNDAASAVTRLTDMGVE
HHHHHHHHCCCCEEEEECCCCCCEEEEEEEEECCCEEEEEEEECCHHHHHHHHHHHCCCC
PFLLSSSLLGVLAQRLVRKLDPTEPSGYRGRTGVFELLVVDDTLRAQIHGQAAEAELRAT
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCHHHHHHCCCHHHHHHHHH
AIAGGMTLMREDGERLVRAGITSPEEVLRVTRE
HHHCCHHHHHHHHHHHHHHCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1738317; 2677007 [H]