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The map label for this gene is purH [H]

Identifier: 120609590

GI number: 120609590

Start: 972692

End: 974293

Strand: Direct

Name: purH [H]

Synonym: Aave_0896

Alternate gene names: 120609590

Gene position: 972692-974293 (Clockwise)

Preceding gene: 120609589

Following gene: 120609594

Centisome position: 18.17

GC content: 67.6

Gene sequence:

>1602_bases
ATGAACGCACTTCTCTCCGTCTCCGACAAGACCGGCATCGTCGAATTCGCCCGGGCTCTGCACGCCCTGGGCATCCGCCT
GCTGTCCACCGGCGGCACCGCCAAGCTGCTGGCCGAGCAGGGTCTGCCCGTCACGGAAGTGGCCGAGGTGACCCAGTTCC
CCGAAATGCTCGACGGCCGCGTGAAGACGCTGCACCCCAAGGTGCACGGCGGCCTGCTCGCGCGCCGCGAGCTGCCGGAA
CACATGGCCGCACTGAAGGAGCACGGCATCGACACCATCGACCTGCTGGTGGTGAACCTCTACCCCTTCGAGGCCACCGT
GGCCAAGGCCGGCTGCACGCTGGCCGACGCCATCGAGAACATCGACATCGGCGGCCCCGCCATGGTGCGCAGCGCCGCCA
AGAACTGGAAAGACGTGGGCGTGGTCACCTCGGCCGACCAGTACGACGCCGTGCTGGGTGAACTGAAGGCCGCGGGCAAG
CTGTCCGACAAGCTGCGCTTCGCGCTGTCGGTGGCCGCGTTCAACCGCATCGCGCAGTACGACGGCGCCATCAGCGACTA
CCTCTCCTCCGTCACCTTCGAGGCCGAGAAGCTGTCCGAGGCCTACGTGCCCGAGCGCAATCCCTTCCCGGGCCAGAGCA
ACGGCCAGTTCGTCAAGCTGCAGGACCTGCGCTACGGCGAGAACAGCCACCAGCAGGCGGCCCTCTACCGCGACCTGTAC
CCCGCGCCCGGCTCCATCGTCACGGGCGAGCAGCTGCAGGGCAAGGAACTCAGCTACAACAACATCGCCGACGCCGATGC
CGCGTGGGAGTGCGTGAAGAGCTTCGACGCCGCCGCCTGCGTGATCGTCAAGCATGCCAACCCCTGCGGCGTGGCCGTGG
GCCTGGACGCCGCCGACGCCTACGGCAAGGCCTTCCAGACCGATCCCACCAGCGCCTTCGGCGGCATCATCGCCTTCAAC
CGCCCGGTGGACGCGGCCGCCGCGCAATTGGTCGTGAAGCAGTTCGTCGAGGTGCTGATGGCGCCCGAGTTCACGCCCGA
GGCGCTGGAGATCTTCAAGCCCAAGACCAACGTGCGCCTGCTGAAGATCGCGCTGCCGGAGCACAAGGGCACGACGGCCT
GGAGCCGCGGCCGCAACGCCATGGATGCCAAGCGCATCGGCTCCGGCATGCTGCTGCAGACGGCCGACAACCACGAGCTG
TCGCTGATGGACCTCAAGGTGGTCACCAAGAAGCAGCCCACGCTCGAGGAGATGGAAGACCTGCTGTTCGCCTGGAAGGT
CGCCAAGTACGTCAAGAGCAATGCCATCGTGTTCTGCAAGGGCGGCATGACGATGGGCGTGGGCGCGGGCCAGATGAGCC
GCCTGGATTCGGCGCGCATCGCCAGCATCAAGGCCGAGCACGCCAAGCTGTCGCTGCAGGGCACGGTGGTGGCGAGCGAC
GCCTTCTTCCCGTTCCGCGACGGCCTGGACGTGGTGGTGGATGCGGGCGCGACCTGCGTGATCCAGCCCGGCGGCTCCAT
GCGCGATCAGGAAGTGATCGACGCGGCCGACGAGCGCGGCGTGGCCATGGTGTTCAGCGGCGTGCGCCACTTCCGCCACT
AA

Upstream 100 bases:

>100_bases
CCGCCGAATGGCTGGGGCTGAACCGCAACACCCTGCGCAAGAAGCTCGTGGAGCACAAGCTGCTCTGACCGACCGAATCC
ACAACCTCCCCACCACCGCA

Downstream 100 bases:

>100_bases
GAGCGGCTAACACGACCCTTCTGGCGTCGTTGCCGCATCTTGTCGTACTACTCGTACTGCCTGCGATACTGCGCCTGGCC
AGAACCGCTTCGCTGGGTCG

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 533; Mature: 533

Protein sequence:

>533_residues
MNALLSVSDKTGIVEFARALHALGIRLLSTGGTAKLLAEQGLPVTEVAEVTQFPEMLDGRVKTLHPKVHGGLLARRELPE
HMAALKEHGIDTIDLLVVNLYPFEATVAKAGCTLADAIENIDIGGPAMVRSAAKNWKDVGVVTSADQYDAVLGELKAAGK
LSDKLRFALSVAAFNRIAQYDGAISDYLSSVTFEAEKLSEAYVPERNPFPGQSNGQFVKLQDLRYGENSHQQAALYRDLY
PAPGSIVTGEQLQGKELSYNNIADADAAWECVKSFDAAACVIVKHANPCGVAVGLDAADAYGKAFQTDPTSAFGGIIAFN
RPVDAAAAQLVVKQFVEVLMAPEFTPEALEIFKPKTNVRLLKIALPEHKGTTAWSRGRNAMDAKRIGSGMLLQTADNHEL
SLMDLKVVTKKQPTLEEMEDLLFAWKVAKYVKSNAIVFCKGGMTMGVGAGQMSRLDSARIASIKAEHAKLSLQGTVVASD
AFFPFRDGLDVVVDAGATCVIQPGGSMRDQEVIDAADERGVAMVFSGVRHFRH

Sequences:

>Translated_533_residues
MNALLSVSDKTGIVEFARALHALGIRLLSTGGTAKLLAEQGLPVTEVAEVTQFPEMLDGRVKTLHPKVHGGLLARRELPE
HMAALKEHGIDTIDLLVVNLYPFEATVAKAGCTLADAIENIDIGGPAMVRSAAKNWKDVGVVTSADQYDAVLGELKAAGK
LSDKLRFALSVAAFNRIAQYDGAISDYLSSVTFEAEKLSEAYVPERNPFPGQSNGQFVKLQDLRYGENSHQQAALYRDLY
PAPGSIVTGEQLQGKELSYNNIADADAAWECVKSFDAAACVIVKHANPCGVAVGLDAADAYGKAFQTDPTSAFGGIIAFN
RPVDAAAAQLVVKQFVEVLMAPEFTPEALEIFKPKTNVRLLKIALPEHKGTTAWSRGRNAMDAKRIGSGMLLQTADNHEL
SLMDLKVVTKKQPTLEEMEDLLFAWKVAKYVKSNAIVFCKGGMTMGVGAGQMSRLDSARIASIKAEHAKLSLQGTVVASD
AFFPFRDGLDVVVDAGATCVIQPGGSMRDQEVIDAADERGVAMVFSGVRHFRH
>Mature_533_residues
MNALLSVSDKTGIVEFARALHALGIRLLSTGGTAKLLAEQGLPVTEVAEVTQFPEMLDGRVKTLHPKVHGGLLARRELPE
HMAALKEHGIDTIDLLVVNLYPFEATVAKAGCTLADAIENIDIGGPAMVRSAAKNWKDVGVVTSADQYDAVLGELKAAGK
LSDKLRFALSVAAFNRIAQYDGAISDYLSSVTFEAEKLSEAYVPERNPFPGQSNGQFVKLQDLRYGENSHQQAALYRDLY
PAPGSIVTGEQLQGKELSYNNIADADAAWECVKSFDAAACVIVKHANPCGVAVGLDAADAYGKAFQTDPTSAFGGIIAFN
RPVDAAAAQLVVKQFVEVLMAPEFTPEALEIFKPKTNVRLLKIALPEHKGTTAWSRGRNAMDAKRIGSGMLLQTADNHEL
SLMDLKVVTKKQPTLEEMEDLLFAWKVAKYVKSNAIVFCKGGMTMGVGAGQMSRLDSARIASIKAEHAKLSLQGTVVASD
AFFPFRDGLDVVVDAGATCVIQPGGSMRDQEVIDAADERGVAMVFSGVRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=495, Percent_Identity=35.1515151515151, Blast_Score=249, Evalue=3e-66,
Organism=Escherichia coli, GI1790439, Length=535, Percent_Identity=57.0093457943925, Blast_Score=595, Evalue=1e-171,
Organism=Caenorhabditis elegans, GI71985564, Length=520, Percent_Identity=32.8846153846154, Blast_Score=236, Evalue=3e-62,
Organism=Caenorhabditis elegans, GI71985574, Length=390, Percent_Identity=25.1282051282051, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71985556, Length=130, Percent_Identity=34.6153846153846, Blast_Score=76, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6323056, Length=494, Percent_Identity=35.4251012145749, Blast_Score=266, Evalue=7e-72,
Organism=Saccharomyces cerevisiae, GI6323768, Length=499, Percent_Identity=34.2685370741483, Blast_Score=256, Evalue=4e-69,
Organism=Drosophila melanogaster, GI24649832, Length=494, Percent_Identity=36.8421052631579, Blast_Score=269, Evalue=5e-72,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 57246; Mature: 57246

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNALLSVSDKTGIVEFARALHALGIRLLSTGGTAKLLAEQGLPVTEVAEVTQFPEMLDGR
CCCEEECCCCCCHHHHHHHHHHHHHEEECCCCCHHHHHHCCCCHHHHHHHHHHHHHHCCC
VKTLHPKVHGGLLARRELPEHMAALKEHGIDTIDLLVVNLYPFEATVAKAGCTLADAIEN
CEEECCHHHCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCHHHHHHCCCHHHHHHHC
IDIGGPAMVRSAAKNWKDVGVVTSADQYDAVLGELKAAGKLSDKLRFALSVAAFNRIAQY
CCCCCHHHHHHHHHCCHHCCEEECCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
DGAISDYLSSVTFEAEKLSEAYVPERNPFPGQSNGQFVKLQDLRYGENSHQQAALYRDLY
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHCC
PAPGSIVTGEQLQGKELSYNNIADADAAWECVKSFDAAACVIVKHANPCGVAVGLDAADA
CCCCCEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCEEEECCCHHH
YGKAFQTDPTSAFGGIIAFNRPVDAAAAQLVVKQFVEVLMAPEFTPEALEIFKPKTNVRL
HCCCCCCCCCHHHCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCEEE
LKIALPEHKGTTAWSRGRNAMDAKRIGSGMLLQTADNHELSLMDLKVVTKKQPTLEEMED
EEEEECCCCCCCHHHCCCCCHHHHHCCCCEEEEECCCCCEEEEEEEEECCCCCCHHHHHH
LLFAWKVAKYVKSNAIVFCKGGMTMGVGAGQMSRLDSARIASIKAEHAKLSLQGTVVASD
HHHHHHHHHHHHCCEEEEEECCCEECCCCCHHHHCCCHHHHHHHHHHHEEEEEEEEEECC
AFFPFRDGLDVVVDAGATCVIQPGGSMRDQEVIDAADERGVAMVFSGVRHFRH
CCCCCCCCCEEEEECCCEEEECCCCCCCHHHHHHHHCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MNALLSVSDKTGIVEFARALHALGIRLLSTGGTAKLLAEQGLPVTEVAEVTQFPEMLDGR
CCCEEECCCCCCHHHHHHHHHHHHHEEECCCCCHHHHHHCCCCHHHHHHHHHHHHHHCCC
VKTLHPKVHGGLLARRELPEHMAALKEHGIDTIDLLVVNLYPFEATVAKAGCTLADAIEN
CEEECCHHHCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCHHHHHHCCCHHHHHHHC
IDIGGPAMVRSAAKNWKDVGVVTSADQYDAVLGELKAAGKLSDKLRFALSVAAFNRIAQY
CCCCCHHHHHHHHHCCHHCCEEECCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
DGAISDYLSSVTFEAEKLSEAYVPERNPFPGQSNGQFVKLQDLRYGENSHQQAALYRDLY
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHCC
PAPGSIVTGEQLQGKELSYNNIADADAAWECVKSFDAAACVIVKHANPCGVAVGLDAADA
CCCCCEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCEEEECCCHHH
YGKAFQTDPTSAFGGIIAFNRPVDAAAAQLVVKQFVEVLMAPEFTPEALEIFKPKTNVRL
HCCCCCCCCCHHHCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCEEE
LKIALPEHKGTTAWSRGRNAMDAKRIGSGMLLQTADNHELSLMDLKVVTKKQPTLEEMED
EEEEECCCCCCCHHHCCCCCHHHHHCCCCEEEEECCCCCEEEEEEEEECCCCCCHHHHHH
LLFAWKVAKYVKSNAIVFCKGGMTMGVGAGQMSRLDSARIASIKAEHAKLSLQGTVVASD
HHHHHHHHHHHHCCEEEEEECCCEECCCCCHHHHCCCHHHHHHHHHHHEEEEEEEEEECC
AFFPFRDGLDVVVDAGATCVIQPGGSMRDQEVIDAADERGVAMVFSGVRHFRH
CCCCCCCCCEEEEECCCEEEECCCCCCCHHHHHHHHCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA