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The map label for this gene is glcF [H]

Identifier: 120608947

GI number: 120608947

Start: 274115

End: 275374

Strand: Direct

Name: glcF [H]

Synonym: Aave_0244

Alternate gene names: 120608947

Gene position: 274115-275374 (Clockwise)

Preceding gene: 120608946

Following gene: 120608948

Centisome position: 5.12

GC content: 71.83

Gene sequence:

>1260_bases
ATGCAAACCCGACTCGCCCCCGAATTCCAGGACACGCCCGAAGGCCGCGAGGCCGAGGGCATCCTGCGCAAGTGCGTGCA
TTGCGGCTTCTGTACCGCCACGTGCCCGACCTACCAGTTGCTCGGCGACGAGCTGGACGGGCCGCGCGGGCGCATCTACC
TCATCAAGCAGGTGCTGGAAGGGGAGACGCCCACGCGCAAGACGCAGCTGCACCTGGACCGCTGCCTGACCTGCCGCAAC
TGCGAGACGACCTGCCCCAGCGGCGTGCAGTACGGGCGCCTGGTGGATATCGGCCGGCGCATCGTCGAGGACAAGGTGCC
GCGGCCGCTGCCCGAGCGCGCGCGCCGCTGGGCGTTGAAGGAGGGGCTGCCCTCGCCGCTGTTCAAGCCGGCGCTGAAGG
CCGGCCAGGCCGTGCGCGGGCTGCTGCCCGCCACGCTGCGCGCCAAGGTGCCGCGCCCCCAGGAGGCGGGCGCCTGGCCG
GTGCGCGAGCACGCGCGCAAGGTGCTCATGCTCGCGGGCTGCGTGCAGCCGGCGATGCTGCCCAACGTGAACCGCGCCAC
CGCGCGCGTGCTGGATGCCGCGGGCATCCAGACCGTGATCGCCGCCGGCGAGGGCTGTTGCGGCGCGGTGAAGTTCCACC
TCAACGACCAGGACGGCGGCCTCGCGCAGATGCGCACCAATATCGATGCCTGGTGGCCCCACGTGGAGGCGGGGGGCGTG
GAAGCGATCGTGCTCAATGCCTCGGGCTGCGGCGCCACGGTGAAGGAGTACGGCCACCTGCTGCGGCATGATCCGGCCTA
TGCCGCGCGGGCGGAGCGCGTCAGCGCGCTGGCCAGGGATCTTTCCGAACTGCTGCCGGACATGCTGCCCGCGCTCGCCG
AGCGCCTGGAAGGGCGCATCGATGCTCCGAAGGCGCTGCTGGCCTACCACCCGCCCTGCACGCTGCAGCACGGGCAGAAG
CTGCGCGGCGGCGTGGAGACCTATTTGGCTCGCCTGGGCTTTCGCCTGCGCGTGGCGCGCACCGAATCGCATCTGTGCTG
CGGATCGGCCGGCACCTATTCGCTGCTGCAACCGGAGATCGCCGGCCAGTTGCGCGACCGCAAGCTCGGCGCGCTGGACG
AGGCCTGCGCGGAGGAGCCGCCCGCGGCCATCCTCTCGGCCAATATCGGCTGCATCACGCACCTGCAGAGCGGCACGGCC
ACGCCGGTGCGGCACTGGATCGAGGTGCTGGACGAGGCGCTGCGGCCCGGTGCGGTGTGA

Upstream 100 bases:

>100_bases
ATCGGATTTCGGGCACCTGGCCGCGCTGTGGCGCACGCGCCGCCACCGCCATTGAGCGCCCTGCGGGCCGACACCACGAC
ATTCCCACGACACGACCACC

Downstream 100 bases:

>100_bases
CGTGCTTGGAGTTTTTTCCGCCGCAGGGGCCGGGCCGGCCGTGCGCCGGAACCTCGGTAAAACCGGTGCCATGGCCGAGG
CTGGCATGATGGCGGGTTTT

Product: glycolate oxidase iron-sulfur subunit

Products: 2-oxo acid; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MQTRLAPEFQDTPEGREAEGILRKCVHCGFCTATCPTYQLLGDELDGPRGRIYLIKQVLEGETPTRKTQLHLDRCLTCRN
CETTCPSGVQYGRLVDIGRRIVEDKVPRPLPERARRWALKEGLPSPLFKPALKAGQAVRGLLPATLRAKVPRPQEAGAWP
VREHARKVLMLAGCVQPAMLPNVNRATARVLDAAGIQTVIAAGEGCCGAVKFHLNDQDGGLAQMRTNIDAWWPHVEAGGV
EAIVLNASGCGATVKEYGHLLRHDPAYAARAERVSALARDLSELLPDMLPALAERLEGRIDAPKALLAYHPPCTLQHGQK
LRGGVETYLARLGFRLRVARTESHLCCGSAGTYSLLQPEIAGQLRDRKLGALDEACAEEPPAAILSANIGCITHLQSGTA
TPVRHWIEVLDEALRPGAV

Sequences:

>Translated_419_residues
MQTRLAPEFQDTPEGREAEGILRKCVHCGFCTATCPTYQLLGDELDGPRGRIYLIKQVLEGETPTRKTQLHLDRCLTCRN
CETTCPSGVQYGRLVDIGRRIVEDKVPRPLPERARRWALKEGLPSPLFKPALKAGQAVRGLLPATLRAKVPRPQEAGAWP
VREHARKVLMLAGCVQPAMLPNVNRATARVLDAAGIQTVIAAGEGCCGAVKFHLNDQDGGLAQMRTNIDAWWPHVEAGGV
EAIVLNASGCGATVKEYGHLLRHDPAYAARAERVSALARDLSELLPDMLPALAERLEGRIDAPKALLAYHPPCTLQHGQK
LRGGVETYLARLGFRLRVARTESHLCCGSAGTYSLLQPEIAGQLRDRKLGALDEACAEEPPAAILSANIGCITHLQSGTA
TPVRHWIEVLDEALRPGAV
>Mature_419_residues
MQTRLAPEFQDTPEGREAEGILRKCVHCGFCTATCPTYQLLGDELDGPRGRIYLIKQVLEGETPTRKTQLHLDRCLTCRN
CETTCPSGVQYGRLVDIGRRIVEDKVPRPLPERARRWALKEGLPSPLFKPALKAGQAVRGLLPATLRAKVPRPQEAGAWP
VREHARKVLMLAGCVQPAMLPNVNRATARVLDAAGIQTVIAAGEGCCGAVKFHLNDQDGGLAQMRTNIDAWWPHVEAGGV
EAIVLNASGCGATVKEYGHLLRHDPAYAARAERVSALARDLSELLPDMLPALAERLEGRIDAPKALLAYHPPCTLQHGQK
LRGGVETYLARLGFRLRVARTESHLCCGSAGTYSLLQPEIAGQLRDRKLGALDEACAEEPPAAILSANIGCITHLQSGTA
TPVRHWIEVLDEALRPGAV

Specific function: Unknown

COG id: COG0247

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI48994913, Length=417, Percent_Identity=58.273381294964, Blast_Score=452, Evalue=1e-128,
Organism=Escherichia coli, GI1786497, Length=252, Percent_Identity=25, Blast_Score=72, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR004017
- InterPro:   IPR012285
- InterPro:   IPR012257 [H]

Pfam domain/function: PF02754 CCG [H]

EC number: 1.1.3.15

Molecular weight: Translated: 45487; Mature: 45487

Theoretical pI: Translated: 8.21; Mature: 8.21

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.1 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
4.1 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTRLAPEFQDTPEGREAEGILRKCVHCGFCTATCPTYQLLGDELDGPRGRIYLIKQVLE
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHC
GETPTRKTQLHLDRCLTCRNCETTCPSGVQYGRLVDIGRRIVEDKVPRPLPERARRWALK
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
EGLPSPLFKPALKAGQAVRGLLPATLRAKVPRPQEAGAWPVREHARKVLMLAGCVQPAML
HCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHC
PNVNRATARVLDAAGIQTVIAAGEGCCGAVKFHLNDQDGGLAQMRTNIDAWWPHVEAGGV
CCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHCCHHHCCCCCCCCE
EAIVLNASGCGATVKEYGHLLRHDPAYAARAERVSALARDLSELLPDMLPALAERLEGRI
EEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DAPKALLAYHPPCTLQHGQKLRGGVETYLARLGFRLRVARTESHLCCGSAGTYSLLQPEI
CCCHHHEEECCCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCEEECCCCCHHHHCHHH
AGQLRDRKLGALDEACAEEPPAAILSANIGCITHLQSGTATPVRHWIEVLDEALRPGAV
HHHHHHHHCCHHHHHHHCCCCHHHHHCCCHHEEECCCCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MQTRLAPEFQDTPEGREAEGILRKCVHCGFCTATCPTYQLLGDELDGPRGRIYLIKQVLE
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHC
GETPTRKTQLHLDRCLTCRNCETTCPSGVQYGRLVDIGRRIVEDKVPRPLPERARRWALK
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
EGLPSPLFKPALKAGQAVRGLLPATLRAKVPRPQEAGAWPVREHARKVLMLAGCVQPAML
HCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHC
PNVNRATARVLDAAGIQTVIAAGEGCCGAVKFHLNDQDGGLAQMRTNIDAWWPHVEAGGV
CCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHCCHHHCCCCCCCCE
EAIVLNASGCGATVKEYGHLLRHDPAYAARAERVSALARDLSELLPDMLPALAERLEGRI
EEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DAPKALLAYHPPCTLQHGQKLRGGVETYLARLGFRLRVARTESHLCCGSAGTYSLLQPEI
CCCHHHEEECCCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCEEECCCCCHHHHCHHH
AGQLRDRKLGALDEACAEEPPAAILSANIGCITHLQSGTATPVRHWIEVLDEALRPGAV
HHHHHHHHCCHHHHHHHCCCCHHHHHCCCHHEEECCCCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-hydroxy acid; O2

Specific reaction: (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8606183; 9278503 [H]