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The map label for this gene is dctD [H]

Identifier: 120608934

GI number: 120608934

Start: 257925

End: 259304

Strand: Direct

Name: dctD [H]

Synonym: Aave_0230

Alternate gene names: 120608934

Gene position: 257925-259304 (Clockwise)

Preceding gene: 120608933

Following gene: 120608936

Centisome position: 4.82

GC content: 73.55

Gene sequence:

>1380_bases
ATGACCGACGCGCCCCTCCCGGCCGCACCCGCCGGGACCACCCGCCCCGTCTATCTGATCGAGGACGACGCCATGGTGCG
CCGCGCCTATGGCCAGGCCCTCGAACTGGCCCACATCCCCGTGCGCGCGTTTCCCCACGGCCAGGCCGCGCTCGACGCCT
TCGGGCAGGACCCGCCCGCCGTCGTGGTGACCGACATCTGCATGCCGGGCCTGGACGGCATGGAACTGCTGCGTCTGCTG
CGCCAGCGGGATGCCGACCTGCCCGTGGTGCTGGTCACCGGCCACGGCGACGTGGCGATGGCGGTGGAAGCCATGCGCGA
CGGCGCCTACGACTTCATCGAAAAGCCGTTCTCGTCCGAGCGCCTGCTGCTGACCGTGCGCCGGGCGCTGGAGCGGCGCG
CGCTGTCCGACGAACTGCAGCGGCTGCGCGCGTGTGGCGGGGCCGACGCGCTTGCCGGGCTGGTAGGCCAGTCCGCCGGC
ATGGCGGAGGTGCGCCGGCTGGTGTCGGCGCTCGCCCCGCTGGACGTGGACGTGCTGCTCCACGGCGAGACTGGCGCCGG
CAAGGAAGTGGTGGCCCGCGCCCTGCACGCCGCCAGCGGCCGCACCGGCCCGTTCGTGGCCATCAACTGCGGAGCGCTGC
CCGAAGCCATCATCGAGAGCGAACTCTTCGGCCACGAGCCGGGCGCGTTCACGGGCGCAACGCGCCGGCGCATCGGCAAG
ATCGAGTACGCCAACGGGGGCACGCTGCTGCTCGACGAGATCGAATCCATGCCGCCCTCGCTGCAGCTGCGCCTGCTGCG
CGTGCTGCAGGAGCGCGAGATCGAACGCCTGGGCAGCAACCAGCCGGTGCCCGTCACCGCGCGCTTCGTGGCCGCCGCCA
AGGCGGACCTGAAGCAGCTCGCCGAGCGCGGTCAGTTCCGTGCCGACCTGTACTACCGCCTGCACGTGGTCGCGATCGAC
ATTCCGCCGCTGCGCGACCGGCCGCAGGACATTCCGCTGCTCATGGCCCACTTCCTCGCGCAGTCGGCCGCGCGCCACGG
CCGCCCGGTGCCCGGCTGGAGCGACCGCGACCTCGCGGGATGGCTGGCCCACGGCTGGCCCGGCAACGTGCGCGAGCTGC
GCAACGCCGCCGAGCGCCTGTGCCTGGGCCTGCCGGTGCAGCCGCTCGAGGCGGGGGGTGAATCCGCCCCGTCGCTCGCG
GGCCGGGTGGAGGCCTTCGAGCGCAAGCTGCTGCGCGACACCCTGGCCTTGACCCAGGGCAATGTGGCGCGCGCCGCCGA
ACTGCTGCAGATGCCCCGCAAGACGGTGTACGACAAGCTGCAGCGCCACGGGCTCGCGCCCGGCAGCGCCGGCATCGCGC
CGCGCGACGGCGAACGGTAG

Upstream 100 bases:

>100_bases
GGAAGACATTCCCGAGGGAGGCGGCGCCGGGTTCACCGTCACCCTTGCGCTCGCCCGGCCCGACGCCTGAATCCCCTCCG
CTCCAAGGACCTTTCTGCCG

Downstream 100 bases:

>100_bases
GCCGCAACGGCCGAGGCCGGGCCGGTCACGCGGCCTGGCGCAGCACGCGCCGGTACTGCACCGCCTCGGCCACGTGCGCT
GGCCCGACGGATGTCTCGCC

Product: Fis family two component sigma-54 specific transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 459; Mature: 458

Protein sequence:

>459_residues
MTDAPLPAAPAGTTRPVYLIEDDAMVRRAYGQALELAHIPVRAFPHGQAALDAFGQDPPAVVVTDICMPGLDGMELLRLL
RQRDADLPVVLVTGHGDVAMAVEAMRDGAYDFIEKPFSSERLLLTVRRALERRALSDELQRLRACGGADALAGLVGQSAG
MAEVRRLVSALAPLDVDVLLHGETGAGKEVVARALHAASGRTGPFVAINCGALPEAIIESELFGHEPGAFTGATRRRIGK
IEYANGGTLLLDEIESMPPSLQLRLLRVLQEREIERLGSNQPVPVTARFVAAAKADLKQLAERGQFRADLYYRLHVVAID
IPPLRDRPQDIPLLMAHFLAQSAARHGRPVPGWSDRDLAGWLAHGWPGNVRELRNAAERLCLGLPVQPLEAGGESAPSLA
GRVEAFERKLLRDTLALTQGNVARAAELLQMPRKTVYDKLQRHGLAPGSAGIAPRDGER

Sequences:

>Translated_459_residues
MTDAPLPAAPAGTTRPVYLIEDDAMVRRAYGQALELAHIPVRAFPHGQAALDAFGQDPPAVVVTDICMPGLDGMELLRLL
RQRDADLPVVLVTGHGDVAMAVEAMRDGAYDFIEKPFSSERLLLTVRRALERRALSDELQRLRACGGADALAGLVGQSAG
MAEVRRLVSALAPLDVDVLLHGETGAGKEVVARALHAASGRTGPFVAINCGALPEAIIESELFGHEPGAFTGATRRRIGK
IEYANGGTLLLDEIESMPPSLQLRLLRVLQEREIERLGSNQPVPVTARFVAAAKADLKQLAERGQFRADLYYRLHVVAID
IPPLRDRPQDIPLLMAHFLAQSAARHGRPVPGWSDRDLAGWLAHGWPGNVRELRNAAERLCLGLPVQPLEAGGESAPSLA
GRVEAFERKLLRDTLALTQGNVARAAELLQMPRKTVYDKLQRHGLAPGSAGIAPRDGER
>Mature_458_residues
TDAPLPAAPAGTTRPVYLIEDDAMVRRAYGQALELAHIPVRAFPHGQAALDAFGQDPPAVVVTDICMPGLDGMELLRLLR
QRDADLPVVLVTGHGDVAMAVEAMRDGAYDFIEKPFSSERLLLTVRRALERRALSDELQRLRACGGADALAGLVGQSAGM
AEVRRLVSALAPLDVDVLLHGETGAGKEVVARALHAASGRTGPFVAINCGALPEAIIESELFGHEPGAFTGATRRRIGKI
EYANGGTLLLDEIESMPPSLQLRLLRVLQEREIERLGSNQPVPVTARFVAAAKADLKQLAERGQFRADLYYRLHVVAIDI
PPLRDRPQDIPLLMAHFLAQSAARHGRPVPGWSDRDLAGWLAHGWPGNVRELRNAAERLCLGLPVQPLEAGGESAPSLAG
RVEAFERKLLRDTLALTQGNVARAAELLQMPRKTVYDKLQRHGLAPGSAGIAPRDGER

Specific function: Member of the two-component regulatory system dctB/dctD involved in the transport of C4-dicarboxylates. When activated by dctB acts in conjunction with sigma-54 to activate the transcription of dctA [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=453, Percent_Identity=39.0728476821192, Blast_Score=319, Evalue=3e-88,
Organism=Escherichia coli, GI1790437, Length=431, Percent_Identity=39.2111368909513, Blast_Score=287, Evalue=1e-78,
Organism=Escherichia coli, GI1788905, Length=411, Percent_Identity=40.3892944038929, Blast_Score=285, Evalue=5e-78,
Organism=Escherichia coli, GI1790299, Length=466, Percent_Identity=36.9098712446352, Blast_Score=280, Evalue=2e-76,
Organism=Escherichia coli, GI87082117, Length=257, Percent_Identity=50.1945525291829, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI1789087, Length=420, Percent_Identity=37.6190476190476, Blast_Score=226, Evalue=3e-60,
Organism=Escherichia coli, GI1789233, Length=234, Percent_Identity=47.4358974358974, Blast_Score=213, Evalue=3e-56,
Organism=Escherichia coli, GI87081872, Length=324, Percent_Identity=39.8148148148148, Blast_Score=211, Evalue=1e-55,
Organism=Escherichia coli, GI1786524, Length=318, Percent_Identity=40.8805031446541, Blast_Score=207, Evalue=1e-54,
Organism=Escherichia coli, GI87082152, Length=318, Percent_Identity=38.9937106918239, Blast_Score=201, Evalue=9e-53,
Organism=Escherichia coli, GI1787583, Length=321, Percent_Identity=37.3831775700935, Blast_Score=181, Evalue=9e-47,
Organism=Escherichia coli, GI87081858, Length=287, Percent_Identity=31.3588850174216, Blast_Score=133, Evalue=3e-32,
Organism=Escherichia coli, GI1789828, Length=213, Percent_Identity=36.150234741784, Blast_Score=121, Evalue=8e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 49529; Mature: 49397

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4 ; PS00758 ARGE_DAPE_CPG2_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDAPLPAAPAGTTRPVYLIEDDAMVRRAYGQALELAHIPVRAFPHGQAALDAFGQDPPA
CCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCC
VVVTDICMPGLDGMELLRLLRQRDADLPVVLVTGHGDVAMAVEAMRDGAYDFIEKPFSSE
EEEEHHHCCCCCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHCCCHHHHHCCCCCC
RLLLTVRRALERRALSDELQRLRACGGADALAGLVGQSAGMAEVRRLVSALAPLDVDVLL
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEE
HGETGAGKEVVARALHAASGRTGPFVAINCGALPEAIIESELFGHEPGAFTGATRRRIGK
ECCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCHHHHHHCC
IEYANGGTLLLDEIESMPPSLQLRLLRVLQEREIERLGSNQPVPVTARFVAAAKADLKQL
EEECCCCEEEHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
AERGQFRADLYYRLHVVAIDIPPLRDRPQDIPLLMAHFLAQSAARHGRPVPGWSDRDLAG
HHCCCCCEEEEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
WLAHGWPGNVRELRNAAERLCLGLPVQPLEAGGESAPSLAGRVEAFERKLLRDTLALTQG
HHHCCCCCCHHHHHHHHHHHHHCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
NVARAAELLQMPRKTVYDKLQRHGLAPGSAGIAPRDGER
CHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TDAPLPAAPAGTTRPVYLIEDDAMVRRAYGQALELAHIPVRAFPHGQAALDAFGQDPPA
CCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCC
VVVTDICMPGLDGMELLRLLRQRDADLPVVLVTGHGDVAMAVEAMRDGAYDFIEKPFSSE
EEEEHHHCCCCCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHCCCHHHHHCCCCCC
RLLLTVRRALERRALSDELQRLRACGGADALAGLVGQSAGMAEVRRLVSALAPLDVDVLL
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEE
HGETGAGKEVVARALHAASGRTGPFVAINCGALPEAIIESELFGHEPGAFTGATRRRIGK
ECCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCHHHHHHCC
IEYANGGTLLLDEIESMPPSLQLRLLRVLQEREIERLGSNQPVPVTARFVAAAKADLKQL
EEECCCCEEEHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
AERGQFRADLYYRLHVVAIDIPPLRDRPQDIPLLMAHFLAQSAARHGRPVPGWSDRDLAG
HHCCCCCEEEEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
WLAHGWPGNVRELRNAAERLCLGLPVQPLEAGGESAPSLAGRVEAFERKLLRDTLALTQG
HHHCCCCCCHHHHHHHHHHHHHCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
NVARAAELLQMPRKTVYDKLQRHGLAPGSAGIAPRDGER
CHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2134335; 2793824; 11481431 [H]