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The map label for this gene is dctB [H]

Identifier: 120608933

GI number: 120608933

Start: 255957

End: 257894

Strand: Direct

Name: dctB [H]

Synonym: Aave_0229

Alternate gene names: 120608933

Gene position: 255957-257894 (Clockwise)

Preceding gene: 120608928

Following gene: 120608934

Centisome position: 4.78

GC content: 72.39

Gene sequence:

>1938_bases
GTGGCCTGCCCGATTGTGCGGTTTTCCGCAATCGACGCCTTGTCAACGGGAGTAGCCCTCGGCGGCCCCGCTTTCGCCAC
CCACCACAATCCATGCCGAACACGCTGGCTCCGCTTGAAACCCGACCGCTCTCTCCTGATCCCCGGCCTTGCGGTGCTGG
CCTGCGTGTGCGTCGGTGCCCTGGTGTACCGCGCCACGCTCGACACGCAGCTCTCCCTGCAGCAGCACGCGGCCGCGCAA
CGGCTCGATTTCGCCGCCCAGAGCCTGGAGTCCCTGCTGCACCGCAACGAGGCGCTGCCCGCCCTGCTGGCGCTGGACGG
TGGCCTGGGCCGCGCGCTGCGCGATTCCCGGCCGGACAACCTGGCGACCGCCAATGCCTTCCTGGGGCAGGCGGCCGGCC
TGGCCGATGTGGAGGCGGCCTACCTGATGGATGCCGGCGGCCAGACGGTGGCGGCCAGCAACTGGCGCCTGCCGAGCAGC
TTCGTGGGGCAGAACTACCGGTTCCGCCCCTACTTCGAGGCGGCGATGCAGGGGCGGACGGGGCGCTTCTACGGCGTGGG
CGCGACCACGGGCAAGCCCGGATATTTCATCGCGGCACCCCTGCCGGCCGCTGCGCTCGCCCGGGGTGTCGTGGCCGTCA
AGATTTCGCTCGACGCCTTCGAGGCCTCGCTCGCCGCGAGCGGCGATACCGTCCTGCTGGCCGACCGCGATGGCGTCGTG
TTCGTGAGCACGGAGGCGCAGTGGCGCTACCGCACGCTGGCACCGTTGGGCACCCAGGCCCTGGCCCGGCTGGAGAACGC
CCAGCAGTACGGCCGCCATGCACTGCGGCCGATGGACCATGGCCTGGGCGCCTGGCCGGACGGCGGGGCTCCGATCCGCC
TGCAGCATGCCGGCCTGGAGCGCGAATTCAGCGTGGCCACGCGCGGCGTCGGGCCGCTGGGCTGGCAGATGCTGCTGCTG
GCGGACCGGTACCCGGTGCGCCAGGAGGCCCTGGTGGCCGGGCTGGCCGGCGGGCTCTGCGCCGCGCTGCTGCTGGGCAT
GCTGTTCTACCAGCGGCTGGACCAGCAGGGCCAGGCGGAGCGCCGCGCGAGCGCCGCGCGCCTGCAGGCCGCGCACGACA
TGCTGGAGACCCAGATCGCGCAGCGCACCACCGACCTGACGGCCGCCAACGCGGCCCTGCAGCTCCGGGTGAGCGAACTG
CAGCACGCGGAAACCATTCTGCGCGGCACGCGCGATGCGGCCGTGCAGGCCGGCAAGCTGGCGGTGCTCGGCCAGATGGC
CGCCGGCATGAGCCATGAACTGAACCAGCCCCTGGCCGCGCTCCAGACGCTCTCGGACAACGCCGTCGCGCTCGACCGGC
AGGGCCGCCGCGAGGACGTGGCCGAAAACCTGCAGCTCATCGGCCAGCTCGCCGCCCGCATGGGCCGGATCGTGCGCCAG
CTCAAGAGCTTCGTGCGCAAGGAAACGCCCGTGCTCCAGCCCTGCCGCGTGCGCGATGCGCTGGACCATGCCTTGCTGCT
GCTGGCGCAGCGTTGCACGGCCGTGCAGGCGCGCATCGACGTGCAGCCTTTCGACGCCGGGCTGCGCGTGATGGCGGATC
CCACCCGGCTGGAGCAGGTGCTGGTGAACCTGCTGCGCAATGGCCTCGACGCGGTGCAGGACCGGCCCGTGCGCGAGGTG
CGCTTCGCCCCGGCCTCCGATGACGGATGGGTGCGCCTGCGCGTGTCCGATACGGGGGGCGGCATCGCGCCCGAGGTCCG
CGGCAGGCTCTTCGAGCCGTTCTTCACGACCAAGGCGGGCGAGGGGCTCGGGCTGGGGCTCGCGGTTTCGCGCATCATCG
TGGAAGGCATGGGCGGCACCCTCACCGTGGAAGACATTCCCGAGGGAGGCGGCGCCGGGTTCACCGTCACCCTTGCGCTC
GCCCGGCCCGACGCCTGA

Upstream 100 bases:

>100_bases
ATGGATGAGCCGGACGGGCTCGCCATTGCTGGAAAGCGGGGGACTGCGCAGGGCGCGCCGCGGGCAGTCCGCGCACGTAC
CTCGCGGTGGTTTTCCGCAC

Downstream 100 bases:

>100_bases
ATCCCCTCCGCTCCAAGGACCTTTCTGCCGATGACCGACGCGCCCCTCCCGGCCGCACCCGCCGGGACCACCCGCCCCGT
CTATCTGATCGAGGACGACG

Product: histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 645; Mature: 644

Protein sequence:

>645_residues
MACPIVRFSAIDALSTGVALGGPAFATHHNPCRTRWLRLKPDRSLLIPGLAVLACVCVGALVYRATLDTQLSLQQHAAAQ
RLDFAAQSLESLLHRNEALPALLALDGGLGRALRDSRPDNLATANAFLGQAAGLADVEAAYLMDAGGQTVAASNWRLPSS
FVGQNYRFRPYFEAAMQGRTGRFYGVGATTGKPGYFIAAPLPAAALARGVVAVKISLDAFEASLAASGDTVLLADRDGVV
FVSTEAQWRYRTLAPLGTQALARLENAQQYGRHALRPMDHGLGAWPDGGAPIRLQHAGLEREFSVATRGVGPLGWQMLLL
ADRYPVRQEALVAGLAGGLCAALLLGMLFYQRLDQQGQAERRASAARLQAAHDMLETQIAQRTTDLTAANAALQLRVSEL
QHAETILRGTRDAAVQAGKLAVLGQMAAGMSHELNQPLAALQTLSDNAVALDRQGRREDVAENLQLIGQLAARMGRIVRQ
LKSFVRKETPVLQPCRVRDALDHALLLLAQRCTAVQARIDVQPFDAGLRVMADPTRLEQVLVNLLRNGLDAVQDRPVREV
RFAPASDDGWVRLRVSDTGGGIAPEVRGRLFEPFFTTKAGEGLGLGLAVSRIIVEGMGGTLTVEDIPEGGGAGFTVTLAL
ARPDA

Sequences:

>Translated_645_residues
MACPIVRFSAIDALSTGVALGGPAFATHHNPCRTRWLRLKPDRSLLIPGLAVLACVCVGALVYRATLDTQLSLQQHAAAQ
RLDFAAQSLESLLHRNEALPALLALDGGLGRALRDSRPDNLATANAFLGQAAGLADVEAAYLMDAGGQTVAASNWRLPSS
FVGQNYRFRPYFEAAMQGRTGRFYGVGATTGKPGYFIAAPLPAAALARGVVAVKISLDAFEASLAASGDTVLLADRDGVV
FVSTEAQWRYRTLAPLGTQALARLENAQQYGRHALRPMDHGLGAWPDGGAPIRLQHAGLEREFSVATRGVGPLGWQMLLL
ADRYPVRQEALVAGLAGGLCAALLLGMLFYQRLDQQGQAERRASAARLQAAHDMLETQIAQRTTDLTAANAALQLRVSEL
QHAETILRGTRDAAVQAGKLAVLGQMAAGMSHELNQPLAALQTLSDNAVALDRQGRREDVAENLQLIGQLAARMGRIVRQ
LKSFVRKETPVLQPCRVRDALDHALLLLAQRCTAVQARIDVQPFDAGLRVMADPTRLEQVLVNLLRNGLDAVQDRPVREV
RFAPASDDGWVRLRVSDTGGGIAPEVRGRLFEPFFTTKAGEGLGLGLAVSRIIVEGMGGTLTVEDIPEGGGAGFTVTLAL
ARPDA
>Mature_644_residues
ACPIVRFSAIDALSTGVALGGPAFATHHNPCRTRWLRLKPDRSLLIPGLAVLACVCVGALVYRATLDTQLSLQQHAAAQR
LDFAAQSLESLLHRNEALPALLALDGGLGRALRDSRPDNLATANAFLGQAAGLADVEAAYLMDAGGQTVAASNWRLPSSF
VGQNYRFRPYFEAAMQGRTGRFYGVGATTGKPGYFIAAPLPAAALARGVVAVKISLDAFEASLAASGDTVLLADRDGVVF
VSTEAQWRYRTLAPLGTQALARLENAQQYGRHALRPMDHGLGAWPDGGAPIRLQHAGLEREFSVATRGVGPLGWQMLLLA
DRYPVRQEALVAGLAGGLCAALLLGMLFYQRLDQQGQAERRASAARLQAAHDMLETQIAQRTTDLTAANAALQLRVSELQ
HAETILRGTRDAAVQAGKLAVLGQMAAGMSHELNQPLAALQTLSDNAVALDRQGRREDVAENLQLIGQLAARMGRIVRQL
KSFVRKETPVLQPCRVRDALDHALLLLAQRCTAVQARIDVQPFDAGLRVMADPTRLEQVLVNLLRNGLDAVQDRPVREVR
FAPASDDGWVRLRVSDTGGGIAPEVRGRLFEPFFTTKAGEGLGLGLAVSRIIVEGMGGTLTVEDIPEGGGAGFTVTLALA
RPDA

Specific function: Member of the two-component regulatory system dctB/dctD involved in the transport of C4-dicarboxylates. DctB functions as a membrane-associated protein kinase that phosphorylates dctD in response to environmental signals [H]

COG id: COG4191

COG function: function code T; Signal transduction histidine kinase regulating C4-dicarboxylate transport system

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=229, Percent_Identity=34.9344978165939, Blast_Score=115, Evalue=1e-26,
Organism=Escherichia coli, GI1788549, Length=236, Percent_Identity=31.7796610169492, Blast_Score=99, Evalue=8e-22,
Organism=Escherichia coli, GI1789149, Length=290, Percent_Identity=25.8620689655172, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI145693157, Length=125, Percent_Identity=35.2, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI1787894, Length=226, Percent_Identity=29.2035398230088, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI87081816, Length=273, Percent_Identity=28.9377289377289, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1788713, Length=243, Percent_Identity=25.9259259259259, Blast_Score=65, Evalue=2e-11,
Organism=Escherichia coli, GI1790346, Length=218, Percent_Identity=28.8990825688073, Blast_Score=63, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR017055
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 68863; Mature: 68732

Theoretical pI: Translated: 8.66; Mature: 8.66

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MACPIVRFSAIDALSTGVALGGPAFATHHNPCRTRWLRLKPDRSLLIPGLAVLACVCVGA
CCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHCCEEEECCCCCEECCHHHHHHHHHHHH
LVYRATLDTQLSLQQHAAAQRLDFAAQSLESLLHRNEALPALLALDGGLGRALRDSRPDN
HHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHCCCCCC
LATANAFLGQAAGLADVEAAYLMDAGGQTVAASNWRLPSSFVGQNYRFRPYFEAAMQGRT
HHHHHHHHHHHCCCCHHHHHHHCCCCCCEEEECCCCCCHHHHCCCCCCCHHHHHHHCCCC
GRFYGVGATTGKPGYFIAAPLPAAALARGVVAVKISLDAFEASLAASGDTVLLADRDGVV
CCEEEECCCCCCCCEEEECCCCHHHHHCCEEEEEEEHHHHHHHHCCCCCEEEEECCCCEE
FVSTEAQWRYRTLAPLGTQALARLENAQQYGRHALRPMDHGLGAWPDGGAPIRLQHAGLE
EEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCEEEECCCCC
REFSVATRGVGPLGWQMLLLADRYPVRQEALVAGLAGGLCAALLLGMLFYQRLDQQGQAE
CHHHHHHCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
RRASAARLQAAHDMLETQIAQRTTDLTAANAALQLRVSELQHAETILRGTRDAAVQAGKL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHCCHHHHHHCCHH
AVLGQMAAGMSHELNQPLAALQTLSDNAVALDRQGRREDVAENLQLIGQLAARMGRIVRQ
HHHHHHHHCHHHHHCCHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LKSFVRKETPVLQPCRVRDALDHALLLLAQRCTAVQARIDVQPFDAGLRVMADPTRLEQV
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCEEEECHHHHHHH
LVNLLRNGLDAVQDRPVREVRFAPASDDGWVRLRVSDTGGGIAPEVRGRLFEPFFTTKAG
HHHHHHHHHHHHHCCCCHHEEECCCCCCCEEEEEEECCCCCCCHHHHHHHCCCHHHCCCC
EGLGLGLAVSRIIVEGMGGTLTVEDIPEGGGAGFTVTLALARPDA
CCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEEEEECCCC
>Mature Secondary Structure 
ACPIVRFSAIDALSTGVALGGPAFATHHNPCRTRWLRLKPDRSLLIPGLAVLACVCVGA
CCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHCCEEEECCCCCEECCHHHHHHHHHHHH
LVYRATLDTQLSLQQHAAAQRLDFAAQSLESLLHRNEALPALLALDGGLGRALRDSRPDN
HHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHCCCCCC
LATANAFLGQAAGLADVEAAYLMDAGGQTVAASNWRLPSSFVGQNYRFRPYFEAAMQGRT
HHHHHHHHHHHCCCCHHHHHHHCCCCCCEEEECCCCCCHHHHCCCCCCCHHHHHHHCCCC
GRFYGVGATTGKPGYFIAAPLPAAALARGVVAVKISLDAFEASLAASGDTVLLADRDGVV
CCEEEECCCCCCCCEEEECCCCHHHHHCCEEEEEEEHHHHHHHHCCCCCEEEEECCCCEE
FVSTEAQWRYRTLAPLGTQALARLENAQQYGRHALRPMDHGLGAWPDGGAPIRLQHAGLE
EEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCEEEECCCCC
REFSVATRGVGPLGWQMLLLADRYPVRQEALVAGLAGGLCAALLLGMLFYQRLDQQGQAE
CHHHHHHCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
RRASAARLQAAHDMLETQIAQRTTDLTAANAALQLRVSELQHAETILRGTRDAAVQAGKL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHCCHHHHHHCCHH
AVLGQMAAGMSHELNQPLAALQTLSDNAVALDRQGRREDVAENLQLIGQLAARMGRIVRQ
HHHHHHHHCHHHHHCCHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LKSFVRKETPVLQPCRVRDALDHALLLLAQRCTAVQARIDVQPFDAGLRVMADPTRLEQV
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCEEEECHHHHHHH
LVNLLRNGLDAVQDRPVREVRFAPASDDGWVRLRVSDTGGGIAPEVRGRLFEPFFTTKAG
HHHHHHHHHHHHHCCCCHHEEECCCCCCCEEEEEEECCCCCCCHHHHHHHCCCHHHCCCC
EGLGLGLAVSRIIVEGMGGTLTVEDIPEGGGAGFTVTLALARPDA
CCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2134335; 2793824; 11481431; 2551890; 2695394 [H]