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The map label for this gene is gcvT

Identifier: 120597561

GI number: 120597561

Start: 808924

End: 810018

Strand: Direct

Name: gcvT

Synonym: Sputw3181_0730

Alternate gene names: 120597561

Gene position: 808924-810018 (Clockwise)

Preceding gene: 120597560

Following gene: 120597562

Centisome position: 17.18

GC content: 50.87

Gene sequence:

>1095_bases
ATGGCTAATAAAACTATTCTGTTTAACAAGCACTTAGAATCAAACGCTAAGATGGTCGACTTCCACGGTTGGGATATGCC
CCTCAACTATGGCTCCCAAATCGAAGAACACCACGCAGTACGCCAAGATGCAGGTATGTTCGACGTTTCCCACATGACTG
TGGTTGATGTGATCGGAACCGACGCGTGTGCATTCCTGCGTAAGTTGCTCGCAAACGATGTGGCTAGACTTAAAGTGCCA
GGTAAAGCCTTATACAGTGGCATGTTAGATGAAAATGCCGGCATCATCGACGACCTCATTACTTACTACCTCACCGACAC
TTTCTACCGCGTTGTCGTCAACAGCGCGACCCGCGAAAAAGATTTAGCGTGGATCGCCAAGCAATCCCAAGGTTTTGATA
TCACTGTGACTGAGCGCCCTGAACTGGCGATGATCGCTGTTCAAGGCCCGAATGCAAAAGCCAAAGCCGCCGCCGTATTC
AGCGCAGACCAAAACGCCGCCATCGAAGGTATGAAGCCATTCTTCGGTAAGCAAGCTGGCTCGCTATTTATTGCGACCAC
AGGTTACACAGGTGAAGCGGGCTACGAAATTATCGTCCCAGAAGATGAAGCCCAAGCCTTGTGGCAAGCATTGTTAGATC
AAGGGGTTAAACCTTGTGGTTTAGGTGCCCGTGACACGCTACGTCTCGAAGCCGGTATGAACCTCTACGGCCTAGATATG
GACGAAACCATCAACCCATTGGCCGCCAACATGGGCTGGACCATCGCATGGGAACCAACCGACCGTGACTTTATCGGCCG
CAAGGCGCTTGAAGCCCTGCGCGATGCCGGAACCGACAAATTAGTCGGTCTAGTGATGGAAGAAAAGGGCGTGTTACGCC
ATGATATGCCAGTATTTTTCACCGATGCAGCGGGTGTTGAGCACCAAGGCGTGATCACCAGCGGGACTTTCTCGCCAACA
TTGGGCTATTCTATTGCGATGGCGCGTGTTCCTAACCAGATTGGGGATACGGCAGAAGTCGAAATGCGTAAAAAACGTGT
GGCTGTTAGAGTTGTGGCGCCAAACTTTGTGCGCAACGGTAAACAAGCATTTTAA

Upstream 100 bases:

>100_bases
AATTTGTGACGCAAGGTATAATTTCGCCGCATTTTAGACCCTAGTATCTACACCCTACACTAGGCAGAGACCTGAAAAAG
GCCGCACAAAAGGGATAACA

Downstream 100 bases:

>100_bases
AAAAACGGAACAAGGACCAAGAACAATGAGCAATATTCCGACAGAACTGAAATACGCCTCTTCACACGAATGGATCCGCA
AGGAAGAAGACGGTAGCTAC

Product: glycine cleavage system aminomethyltransferase T

Products: NA

Alternate protein names: Glycine cleavage system T protein [H]

Number of amino acids: Translated: 364; Mature: 363

Protein sequence:

>364_residues
MANKTILFNKHLESNAKMVDFHGWDMPLNYGSQIEEHHAVRQDAGMFDVSHMTVVDVIGTDACAFLRKLLANDVARLKVP
GKALYSGMLDENAGIIDDLITYYLTDTFYRVVVNSATREKDLAWIAKQSQGFDITVTERPELAMIAVQGPNAKAKAAAVF
SADQNAAIEGMKPFFGKQAGSLFIATTGYTGEAGYEIIVPEDEAQALWQALLDQGVKPCGLGARDTLRLEAGMNLYGLDM
DETINPLAANMGWTIAWEPTDRDFIGRKALEALRDAGTDKLVGLVMEEKGVLRHDMPVFFTDAAGVEHQGVITSGTFSPT
LGYSIAMARVPNQIGDTAEVEMRKKRVAVRVVAPNFVRNGKQAF

Sequences:

>Translated_364_residues
MANKTILFNKHLESNAKMVDFHGWDMPLNYGSQIEEHHAVRQDAGMFDVSHMTVVDVIGTDACAFLRKLLANDVARLKVP
GKALYSGMLDENAGIIDDLITYYLTDTFYRVVVNSATREKDLAWIAKQSQGFDITVTERPELAMIAVQGPNAKAKAAAVF
SADQNAAIEGMKPFFGKQAGSLFIATTGYTGEAGYEIIVPEDEAQALWQALLDQGVKPCGLGARDTLRLEAGMNLYGLDM
DETINPLAANMGWTIAWEPTDRDFIGRKALEALRDAGTDKLVGLVMEEKGVLRHDMPVFFTDAAGVEHQGVITSGTFSPT
LGYSIAMARVPNQIGDTAEVEMRKKRVAVRVVAPNFVRNGKQAF
>Mature_363_residues
ANKTILFNKHLESNAKMVDFHGWDMPLNYGSQIEEHHAVRQDAGMFDVSHMTVVDVIGTDACAFLRKLLANDVARLKVPG
KALYSGMLDENAGIIDDLITYYLTDTFYRVVVNSATREKDLAWIAKQSQGFDITVTERPELAMIAVQGPNAKAKAAAVFS
ADQNAAIEGMKPFFGKQAGSLFIATTGYTGEAGYEIIVPEDEAQALWQALLDQGVKPCGLGARDTLRLEAGMNLYGLDMD
ETINPLAANMGWTIAWEPTDRDFIGRKALEALRDAGTDKLVGLVMEEKGVLRHDMPVFFTDAAGVEHQGVITSGTFSPTL
GYSIAMARVPNQIGDTAEVEMRKKRVAVRVVAPNFVRNGKQAF

Specific function: The glycine cleavage system catalyzes the degradation of glycine [H]

COG id: COG0404

COG function: function code E; Glycine cleavage system T protein (aminomethyltransferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gcvT family [H]

Homologues:

Organism=Homo sapiens, GI44662838, Length=373, Percent_Identity=34.5844504021448, Blast_Score=171, Evalue=8e-43,
Organism=Homo sapiens, GI257796258, Length=346, Percent_Identity=34.6820809248555, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI257796254, Length=357, Percent_Identity=31.0924369747899, Blast_Score=135, Evalue=7e-32,
Organism=Homo sapiens, GI257796256, Length=307, Percent_Identity=33.8762214983713, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI24797151, Length=385, Percent_Identity=24.4155844155844, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI197927446, Length=311, Percent_Identity=27.0096463022508, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI21361378, Length=311, Percent_Identity=27.0096463022508, Blast_Score=85, Evalue=1e-16,
Organism=Escherichia coli, GI1789272, Length=361, Percent_Identity=68.9750692520776, Blast_Score=528, Evalue=1e-151,
Organism=Caenorhabditis elegans, GI17560118, Length=374, Percent_Identity=33.9572192513369, Blast_Score=155, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI71994045, Length=345, Percent_Identity=24.3478260869565, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71994052, Length=345, Percent_Identity=24.3478260869565, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6320222, Length=341, Percent_Identity=34.0175953079179, Blast_Score=155, Evalue=1e-38,
Organism=Drosophila melanogaster, GI20129441, Length=377, Percent_Identity=32.8912466843501, Blast_Score=169, Evalue=4e-42,
Organism=Drosophila melanogaster, GI28571104, Length=263, Percent_Identity=24.3346007604563, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI20130091, Length=396, Percent_Identity=22.7272727272727, Blast_Score=72, Evalue=9e-13,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013977
- InterPro:   IPR006222
- InterPro:   IPR006223
- InterPro:   IPR022903 [H]

Pfam domain/function: PF01571 GCV_T; PF08669 GCV_T_C [H]

EC number: =2.1.2.10 [H]

Molecular weight: Translated: 39797; Mature: 39666

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANKTILFNKHLESNAKMVDFHGWDMPLNYGSQIEEHHAVRQDAGMFDVSHMTVVDVIGT
CCCCEEEEECCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCEEECCEEEEEECCC
DACAFLRKLLANDVARLKVPGKALYSGMLDENAGIIDDLITYYLTDTFYRVVVNSATREK
CHHHHHHHHHHCCHHEEECCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHH
DLAWIAKQSQGFDITVTERPELAMIAVQGPNAKAKAAAVFSADQNAAIEGMKPFFGKQAG
HHHHEEECCCCCEEEEECCCCEEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCCCCC
SLFIATTGYTGEAGYEIIVPEDEAQALWQALLDQGVKPCGLGARDTLRLEAGMNLYGLDM
CEEEEECCCCCCCCCEEEECCHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCEEECCH
DETINPLAANMGWTIAWEPTDRDFIGRKALEALRDAGTDKLVGLVMEEKGVLRHDMPVFF
HHHCCHHHHCCCEEEEECCCCHHHHHHHHHHHHHHCCCCHHEEEEECCCCCHHCCCCEEE
TDAAGVEHQGVITSGTFSPTLGYSIAMARVPNQIGDTAEVEMRKKRVAVRVVAPNFVRNG
ECCCCCCCCCEEECCCCCCCCCCEEHHHHCCHHHCCCHHHHHHHHEEEEEEECCHHHCCC
KQAF
CCCC
>Mature Secondary Structure 
ANKTILFNKHLESNAKMVDFHGWDMPLNYGSQIEEHHAVRQDAGMFDVSHMTVVDVIGT
CCCEEEEECCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCEEECCEEEEEECCC
DACAFLRKLLANDVARLKVPGKALYSGMLDENAGIIDDLITYYLTDTFYRVVVNSATREK
CHHHHHHHHHHCCHHEEECCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHH
DLAWIAKQSQGFDITVTERPELAMIAVQGPNAKAKAAAVFSADQNAAIEGMKPFFGKQAG
HHHHEEECCCCCEEEEECCCCEEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCCCCC
SLFIATTGYTGEAGYEIIVPEDEAQALWQALLDQGVKPCGLGARDTLRLEAGMNLYGLDM
CEEEEECCCCCCCCCEEEECCHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCEEECCH
DETINPLAANMGWTIAWEPTDRDFIGRKALEALRDAGTDKLVGLVMEEKGVLRHDMPVFF
HHHCCHHHHCCCEEEEECCCCHHHHHHHHHHHHHHCCCCHHEEEEECCCCCHHCCCCEEE
TDAAGVEHQGVITSGTFSPTLGYSIAMARVPNQIGDTAEVEMRKKRVAVRVVAPNFVRNG
ECCCCCCCCCEEECCCCCCCCCCEEHHHHCCHHHCCCHHHHHHHHEEEEEEECCHHHCCC
KQAF
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA