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The map label for this gene is fliF [H]

Identifier: 119947167

GI number: 119947167

Start: 4383228

End: 4384976

Strand: Reverse

Name: fliF [H]

Synonym: Ping_3565

Alternate gene names: 119947167

Gene position: 4384976-4383228 (Counterclockwise)

Preceding gene: 119947168

Following gene: 119947166

Centisome position: 96.17

GC content: 41.51

Gene sequence:

>1749_bases
ATGGAACACTCAATGCCCGCCCGTAAAAATACACTACCGGGTAACTTAGCAGACAAAAGCGGCGAAGCAAACGGCCGATT
TGTAAATAGAAAAACAGGCAAGGGAGTTGCTGAAGATACCCTTTTTTCGGCATTAAGCGTCTGGGGAAAAATACTTTATT
TGTTAAAAAACTCACGTAAAAGTTTTGCTGTTATCACGGTATTTTTAACTGCACTAGTGACCATACTGGTTATTTTATTA
TGGTCATCCTCAAGCAACTACCGTCCGCTTTACGGTATACAGGAGCCCTATAATAACAGTGATATTCTGGCTGCATTAGA
AGCTGAAAACATAAAATATCAAATGCAGCCCGATACCGGTCTGATTTTAGTTGAAGATGGCGAAGTTGCAAAAATTAGAA
TGTTACTCGCGGCAAAGGGCGTAAAAGCAACACTCCCCGAAGGTTTGAGCGTGCTTGGAAAGCAATCTGCACTAGGTACA
AGTCAGTTTATTGAGAATGCGCGATATAATCTTGGTCTTGAGGGGGAACTGGCTAAAACTATTATGTCACTGAATGCGAT
TAATAATGCCCGAGTGCATTTAGCTATTCCTAAGGAAAATTTGTTCGTCGGCCGAGGCGATAATATTGCGACCGCAGCGG
TAATGCTGGATGTTAAATCCGGCGATAAACTGAGTGTTGCACAGATTACTTCTATTATGAATCTGGTCGCCGGCAGTGTG
AATAATCTCAATCCGGAATCTGTTTCAATTACCGATCAATCGGGTCGTTTACTCAGTGATGATGTTAATGATGGCGAATA
TTCATTAGCGTCGTCTAAACAGTTAGAGTATAAAGCAGGCATTGAACAGCAGATCAAGAAACAAGCAAAATCGATGTTAA
TCCCGATTCTTGGTGAGAATAACTTCCGTATTGAAAGTGCTGCGGATATCGATTTTTCGAAAAAACAAGAAACCATGGAA
AGGTTTGATGCTACGCCAGTTTTGCTCAGCGAAATGAGTATTGAGGATGACGATCAGTCAGATATTGCGATCGGGATTCC
CGGATCATTAAGTAGCACTCCACCGGTGGTTGATGGACAAGAAAGTACACCCAACAACAGTAAAAAGAAATTGCGCCGTG
AATTAAATCGAAAATATGCCGTTGGCAGTTCAGTCACTCATACGGAATATCAGCAGGGCAAGATTAATCATATTTCATTA
TCTGTGGTCATTAATAAAGATATTGCCCCGGATGGTGAGTGGACAGCTGATGAGTTGTCAAAAATAAATGCAATGATCTC
TAGCGCGGTAGGGCTTGATTTGAACAGGGGTGACAGCATTAATGTCACTAGTTTTACATTTTCACCCATTCAGGTCACCA
ATCCCGTTGCTGCAGCCTGGTATGAAGAAACCAGGTGGCAATCGTTACTGAAAATAATTATCAGTGGCTTAGTCGCAATG
ATGCTGATTATTGTGGTGTTAAGACCGTTGATTAAACATATTACTGTTAATAATTCAGCGAAGGATGCCGGACCATTACC
AGCAGAGCATCAAGCAATTGCGCAAAGTACGCAGGATAAAGAAAAAGAATTTGCACAGCGTTTAGCTGAATATGGAATTG
GTGAGACTGCCAGCGGTAATGATTCAATGATGCAGATGCTGCCGAGTCCAAACTCACCGCTTAGTGTTCAAATTCAGCAT
TTGCAGCTTATCTCCAAAGAACACCCAAATCGTGTCAGTGAAATATTGAAAAATTGGGTTAACGCATAA

Upstream 100 bases:

>100_bases
AAAAGCAAGCCTGTCCTTTACCGCTCTAATGCAGGTTCGAAATAAATTGTTATCAAGTTTTGATGAAGTTATGAAGATGT
CAGTTTAATAAGGTTATGTC

Downstream 100 bases:

>100_bases
GGGCTTATGATGGAAAATAAAGATACTACAATAACAGATTCTGATTTACCCAGCGTTTCTAACGAGCCACTTGCCCTTTC
AAAGGTAGAGCAGGCCGCTT

Product: flagellar MS-ring protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 582; Mature: 582

Protein sequence:

>582_residues
MEHSMPARKNTLPGNLADKSGEANGRFVNRKTGKGVAEDTLFSALSVWGKILYLLKNSRKSFAVITVFLTALVTILVILL
WSSSSNYRPLYGIQEPYNNSDILAALEAENIKYQMQPDTGLILVEDGEVAKIRMLLAAKGVKATLPEGLSVLGKQSALGT
SQFIENARYNLGLEGELAKTIMSLNAINNARVHLAIPKENLFVGRGDNIATAAVMLDVKSGDKLSVAQITSIMNLVAGSV
NNLNPESVSITDQSGRLLSDDVNDGEYSLASSKQLEYKAGIEQQIKKQAKSMLIPILGENNFRIESAADIDFSKKQETME
RFDATPVLLSEMSIEDDDQSDIAIGIPGSLSSTPPVVDGQESTPNNSKKKLRRELNRKYAVGSSVTHTEYQQGKINHISL
SVVINKDIAPDGEWTADELSKINAMISSAVGLDLNRGDSINVTSFTFSPIQVTNPVAAAWYEETRWQSLLKIIISGLVAM
MLIIVVLRPLIKHITVNNSAKDAGPLPAEHQAIAQSTQDKEKEFAQRLAEYGIGETASGNDSMMQMLPSPNSPLSVQIQH
LQLISKEHPNRVSEILKNWVNA

Sequences:

>Translated_582_residues
MEHSMPARKNTLPGNLADKSGEANGRFVNRKTGKGVAEDTLFSALSVWGKILYLLKNSRKSFAVITVFLTALVTILVILL
WSSSSNYRPLYGIQEPYNNSDILAALEAENIKYQMQPDTGLILVEDGEVAKIRMLLAAKGVKATLPEGLSVLGKQSALGT
SQFIENARYNLGLEGELAKTIMSLNAINNARVHLAIPKENLFVGRGDNIATAAVMLDVKSGDKLSVAQITSIMNLVAGSV
NNLNPESVSITDQSGRLLSDDVNDGEYSLASSKQLEYKAGIEQQIKKQAKSMLIPILGENNFRIESAADIDFSKKQETME
RFDATPVLLSEMSIEDDDQSDIAIGIPGSLSSTPPVVDGQESTPNNSKKKLRRELNRKYAVGSSVTHTEYQQGKINHISL
SVVINKDIAPDGEWTADELSKINAMISSAVGLDLNRGDSINVTSFTFSPIQVTNPVAAAWYEETRWQSLLKIIISGLVAM
MLIIVVLRPLIKHITVNNSAKDAGPLPAEHQAIAQSTQDKEKEFAQRLAEYGIGETASGNDSMMQMLPSPNSPLSVQIQH
LQLISKEHPNRVSEILKNWVNA
>Mature_582_residues
MEHSMPARKNTLPGNLADKSGEANGRFVNRKTGKGVAEDTLFSALSVWGKILYLLKNSRKSFAVITVFLTALVTILVILL
WSSSSNYRPLYGIQEPYNNSDILAALEAENIKYQMQPDTGLILVEDGEVAKIRMLLAAKGVKATLPEGLSVLGKQSALGT
SQFIENARYNLGLEGELAKTIMSLNAINNARVHLAIPKENLFVGRGDNIATAAVMLDVKSGDKLSVAQITSIMNLVAGSV
NNLNPESVSITDQSGRLLSDDVNDGEYSLASSKQLEYKAGIEQQIKKQAKSMLIPILGENNFRIESAADIDFSKKQETME
RFDATPVLLSEMSIEDDDQSDIAIGIPGSLSSTPPVVDGQESTPNNSKKKLRRELNRKYAVGSSVTHTEYQQGKINHISL
SVVINKDIAPDGEWTADELSKINAMISSAVGLDLNRGDSINVTSFTFSPIQVTNPVAAAWYEETRWQSLLKIIISGLVAM
MLIIVVLRPLIKHITVNNSAKDAGPLPAEHQAIAQSTQDKEKEFAQRLAEYGIGETASGNDSMMQMLPSPNSPLSVQIQH
LQLISKEHPNRVSEILKNWVNA

Specific function: The M ring may be actively involved in energy transduction [H]

COG id: COG1766

COG function: function code NU; Flagellar biosynthesis/type III secretory pathway lipoprotein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein. Bacterial flagellum basal body (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fliF family [H]

Homologues:

Organism=Escherichia coli, GI1788248, Length=419, Percent_Identity=30.7875894988067, Blast_Score=189, Evalue=5e-49,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013556
- InterPro:   IPR000067
- InterPro:   IPR006182 [H]

Pfam domain/function: PF01514 YscJ_FliF; PF08345 YscJ_FliF_C [H]

EC number: NA

Molecular weight: Translated: 63411; Mature: 63411

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEHSMPARKNTLPGNLADKSGEANGRFVNRKTGKGVAEDTLFSALSVWGKILYLLKNSRK
CCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
SFAVITVFLTALVTILVILLWSSSSNYRPLYGIQEPYNNSDILAALEAENIKYQMQPDTG
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEECCEEEEECCCCC
LILVEDGEVAKIRMLLAAKGVKATLPEGLSVLGKQSALGTSQFIENARYNLGLEGELAKT
EEEEECCCHHHHHHHHHHCCCCCCCCHHHHHHHCHHCCCHHHHHHCCCCCCCCCHHHHHH
IMSLNAINNARVHLAIPKENLFVGRGDNIATAAVMLDVKSGDKLSVAQITSIMNLVAGSV
HHHHHCCCCCEEEEEECCCCEEEECCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHCCC
NNLNPESVSITDQSGRLLSDDVNDGEYSLASSKQLEYKAGIEQQIKKQAKSMLIPILGEN
CCCCCCEEEEECCCCCEECCCCCCCCEECCCCCCCHHHCCHHHHHHHHHHHCEEEEECCC
NFRIESAADIDFSKKQETMERFDATPVLLSEMSIEDDDQSDIAIGIPGSLSSTPPVVDGQ
CEEEECCCCCCCHHHHHHHHHHCCCCEEEHHCCCCCCCCCCEEEECCCCCCCCCCCCCCC
ESTPNNSKKKLRRELNRKYAVGSSVTHTEYQQGKINHISLSVVINKDIAPDGEWTADELS
CCCCCHHHHHHHHHHHHHEECCCCCCCCHHHCCCEEEEEEEEEEECCCCCCCCCCHHHHH
KINAMISSAVGLDLNRGDSINVTSFTFSPIQVTNPVAAAWYEETRWQSLLKIIISGLVAM
HHHHHHHHHHCCCCCCCCCEEEEEEEECCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
MLIIVVLRPLIKHITVNNSAKDAGPLPAEHQAIAQSTQDKEKEFAQRLAEYGIGETASGN
HHHHHHHHHHHHHEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
DSMMQMLPSPNSPLSVQIQHLQLISKEHPNRVSEILKNWVNA
HHHHHHCCCCCCCCEEEEHHHHHHHHCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MEHSMPARKNTLPGNLADKSGEANGRFVNRKTGKGVAEDTLFSALSVWGKILYLLKNSRK
CCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
SFAVITVFLTALVTILVILLWSSSSNYRPLYGIQEPYNNSDILAALEAENIKYQMQPDTG
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEECCEEEEECCCCC
LILVEDGEVAKIRMLLAAKGVKATLPEGLSVLGKQSALGTSQFIENARYNLGLEGELAKT
EEEEECCCHHHHHHHHHHCCCCCCCCHHHHHHHCHHCCCHHHHHHCCCCCCCCCHHHHHH
IMSLNAINNARVHLAIPKENLFVGRGDNIATAAVMLDVKSGDKLSVAQITSIMNLVAGSV
HHHHHCCCCCEEEEEECCCCEEEECCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHCCC
NNLNPESVSITDQSGRLLSDDVNDGEYSLASSKQLEYKAGIEQQIKKQAKSMLIPILGEN
CCCCCCEEEEECCCCCEECCCCCCCCEECCCCCCCHHHCCHHHHHHHHHHHCEEEEECCC
NFRIESAADIDFSKKQETMERFDATPVLLSEMSIEDDDQSDIAIGIPGSLSSTPPVVDGQ
CEEEECCCCCCCHHHHHHHHHHCCCCEEEHHCCCCCCCCCCEEEECCCCCCCCCCCCCCC
ESTPNNSKKKLRRELNRKYAVGSSVTHTEYQQGKINHISLSVVINKDIAPDGEWTADELS
CCCCCHHHHHHHHHHHHHEECCCCCCCCHHHCCCEEEEEEEEEEECCCCCCCCCCHHHHH
KINAMISSAVGLDLNRGDSINVTSFTFSPIQVTNPVAAAWYEETRWQSLLKIIISGLVAM
HHHHHHHHHHCCCCCCCCCEEEEEEEECCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
MLIIVVLRPLIKHITVNNSAKDAGPLPAEHQAIAQSTQDKEKEFAQRLAEYGIGETASGN
HHHHHHHHHHHHHEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
DSMMQMLPSPNSPLSVQIQHLQLISKEHPNRVSEILKNWVNA
HHHHHHCCCCCCCCEEEEHHHHHHHHCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8675317; 10984043 [H]