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The map label for this gene is rpoA2

Identifier: 119947101

GI number: 119947101

Start: 4330000

End: 4330986

Strand: Reverse

Name: rpoA2

Synonym: Ping_3499

Alternate gene names: 119947101

Gene position: 4330986-4330000 (Counterclockwise)

Preceding gene: 119947102

Following gene: 119947100

Centisome position: 94.99

GC content: 42.25

Gene sequence:

>987_bases
ATGCAGGGTTCTGTAATTGAATTTCTAAAACCAAATTTAGTTGGTATCGAACAAATAAATGCAACTAGAGCAAAAGTGAC
TTTAGGACCGTTAGAACGCGGTTTTGGTCATACTTTAGGTAATGCGCTACGTCGTATTCTGTTATCCTCTATGCCCGGCA
CAGCGGTAACAGAAGTCGAAATTGATGGTGTACAACATGAGTACAGCACTAAAGAGGGCGTGCAGGAAGATATCCTTGAG
ATACTTCTTAACCTTAAAGGTCTAGCGGTTAAACTTGAAGGTAAAGACAATGTTCTAGTCAGCCTAACTAAATCTGGGGC
CGGTCCTGTAACTGCAGGTGACATCACTCATGGTAGTGATGTAGAGATAGTCAACCCAGAACATGTGATCTGTCATTTAA
CAGGCAATGCTGAAATCAGCATGCGTATTAAAATTGAATCAGGTCGCGGTTATGTTCCAGCTTCATCACGTATTCATACT
GAAGAAGATGAGCGTCCAATTGGTCGTTTACTAGTAGACGCGACTTTCAGCCCTGTTGAGCGTATCGCTTATAGTGTTGA
ATCTGCTCGTGTTGAACAGCGTACCGATTTAGACAAACTTGTTATCGATATGGAAACCGATGGGACACTTGATCCGGAAG
AAGCGATTCGTCGTGCAGCCACTATTTTGGCTGAACAATTAGACGCATTCGTTGATTTACGTAAAGTCAGTGAGCCAGTC
GCTAAGGAAGAAAAACCTGAGTTTGATCCTATTCTCTTACGCCCAGTAGATGATCTAGAATTAACTGTTCGTTCTGCTAA
CTGTTTGAAAGCAGAAACGATTCACTATATCGGTGACTTGGTACAAAGAACAGAAGTAGAGCTTCTTAAAACGCCTAATT
TAGGTAAGAAGTCACTTACTGAAATCAAAGATGTTCTTGCATCACGTGGACTTTCTCTGGGTATGCGCCTGGAAAATTGG
CCACCAGCAAGCCTCAGCGAAGATTAA

Upstream 100 bases:

>100_bases
ACTCGTCAACCTGAACGTTCAGATTTATCTGCTGAGATTAACGAACAGTTAATCATTGAACTTTACTCTAAGTAAAGTTA
ACCGATAAGAGAGGGCACAA

Downstream 100 bases:

>100_bases
TCAGTCATCCGAATCATGAAAAATAAGGATTAGGTTATGCGCCATCGTCAAAGTGGACGTCAACTTAACCGAAACAGTAG
CCACCGTCAGGCTATGTTTC

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha 2; RNA polymerase subunit alpha 2; Transcriptase subunit alpha 2

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MQGSVIEFLKPNLVGIEQINATRAKVTLGPLERGFGHTLGNALRRILLSSMPGTAVTEVEIDGVQHEYSTKEGVQEDILE
ILLNLKGLAVKLEGKDNVLVSLTKSGAGPVTAGDITHGSDVEIVNPEHVICHLTGNAEISMRIKIESGRGYVPASSRIHT
EEDERPIGRLLVDATFSPVERIAYSVESARVEQRTDLDKLVIDMETDGTLDPEEAIRRAATILAEQLDAFVDLRKVSEPV
AKEEKPEFDPILLRPVDDLELTVRSANCLKAETIHYIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENW
PPASLSED

Sequences:

>Translated_328_residues
MQGSVIEFLKPNLVGIEQINATRAKVTLGPLERGFGHTLGNALRRILLSSMPGTAVTEVEIDGVQHEYSTKEGVQEDILE
ILLNLKGLAVKLEGKDNVLVSLTKSGAGPVTAGDITHGSDVEIVNPEHVICHLTGNAEISMRIKIESGRGYVPASSRIHT
EEDERPIGRLLVDATFSPVERIAYSVESARVEQRTDLDKLVIDMETDGTLDPEEAIRRAATILAEQLDAFVDLRKVSEPV
AKEEKPEFDPILLRPVDDLELTVRSANCLKAETIHYIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENW
PPASLSED
>Mature_328_residues
MQGSVIEFLKPNLVGIEQINATRAKVTLGPLERGFGHTLGNALRRILLSSMPGTAVTEVEIDGVQHEYSTKEGVQEDILE
ILLNLKGLAVKLEGKDNVLVSLTKSGAGPVTAGDITHGSDVEIVNPEHVICHLTGNAEISMRIKIESGRGYVPASSRIHT
EEDERPIGRLLVDATFSPVERIAYSVESARVEQRTDLDKLVIDMETDGTLDPEEAIRRAATILAEQLDAFVDLRKVSEPV
AKEEKPEFDPILLRPVDDLELTVRSANCLKAETIHYIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENW
PPASLSED

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family

Homologues:

Organism=Escherichia coli, GI1789690, Length=329, Percent_Identity=84.8024316109423, Blast_Score=567, Evalue=1e-163,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): RPOA2_PSYIN (A1T0B7)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_944781.1
- ProteinModelPortal:   A1T0B7
- STRING:   A1T0B7
- GeneID:   4625533
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_3499
- eggNOG:   COG0202
- HOGENOM:   HBG430844
- OMA:   FGTTLGN
- PhylomeDB:   A1T0B7
- ProtClustDB:   PRK05182
- BioCyc:   PING357804:PING_3499-MONOMER
- HAMAP:   MF_00059
- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- Gene3D:   G3DSA:2.170.120.12
- ProDom:   PD001179
- SMART:   SM00662
- TIGRFAMs:   TIGR02027

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L; SSF47789 RNAP_alpha_C; SSF56553 RNAP_insert; SSF55257 RNAP_RBP11-like

EC number: =2.7.7.6

Molecular weight: Translated: 35920; Mature: 35920

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQGSVIEFLKPNLVGIEQINATRAKVTLGPLERGFGHTLGNALRRILLSSMPGTAVTEVE
CCCCCHHHHCCCEEEHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
IDGVQHEYSTKEGVQEDILEILLNLKGLAVKLEGKDNVLVSLTKSGAGPVTAGDITHGSD
ECCCCCHHCCCCCHHHHHHHHHHCCCCEEEEEECCCCEEEEEECCCCCCCEECCCCCCCC
VEIVNPEHVICHLTGNAEISMRIKIESGRGYVPASSRIHTEEDERPIGRLLVDATFSPVE
EEEECCCEEEEEEECCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHEEECCCCCHHH
RIAYSVESARVEQRTDLDKLVIDMETDGTLDPEEAIRRAATILAEQLDAFVDLRKVSEPV
HHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKEEKPEFDPILLRPVDDLELTVRSANCLKAETIHYIGDLVQRTEVELLKTPNLGKKSLT
HHCCCCCCCCEEECCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
EIKDVLASRGLSLGMRLENWPPASLSED
HHHHHHHHCCCEECCEECCCCCCCCCCC
>Mature Secondary Structure
MQGSVIEFLKPNLVGIEQINATRAKVTLGPLERGFGHTLGNALRRILLSSMPGTAVTEVE
CCCCCHHHHCCCEEEHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
IDGVQHEYSTKEGVQEDILEILLNLKGLAVKLEGKDNVLVSLTKSGAGPVTAGDITHGSD
ECCCCCHHCCCCCHHHHHHHHHHCCCCEEEEEECCCCEEEEEECCCCCCCEECCCCCCCC
VEIVNPEHVICHLTGNAEISMRIKIESGRGYVPASSRIHTEEDERPIGRLLVDATFSPVE
EEEECCCEEEEEEECCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHEEECCCCCHHH
RIAYSVESARVEQRTDLDKLVIDMETDGTLDPEEAIRRAATILAEQLDAFVDLRKVSEPV
HHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKEEKPEFDPILLRPVDDLELTVRSANCLKAETIHYIGDLVQRTEVELLKTPNLGKKSLT
HHCCCCCCCCEEECCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
EIKDVLASRGLSLGMRLENWPPASLSED
HHHHHHHHCCCEECCEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA